• Nuclear Engineering and Technology
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• 제56권2호
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• pp.536-545
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• 2024

Radioactive iodine is a representative fission product to be quantified for the safety assessment of nuclear facilities. In integral severe accident analysis codes, the iodine behavior is usually described by a multi-physical model of iodine chemistry in aqueous phase under radiation field and mass transfer through gas-liquid interface. The focus of studies on iodine source term evaluations using the combination approach is usually put on the chemical aspect, but each contribution to the iodine amount released to the environment has not been decomposed so far. In this study, we attempted the decomposition by revising the two-film theory of molecular-iodine mass transfer. The model involves an effective overall mass transfer coefficient to consider the iodine chemistry. The decomposition was performed by regarding the coefficient as a product of two functions of pH and the overall mass transfer coefficient for molecular iodine. The procedure was applied to the EPICUR experiment and suppression chamber in BWR.

• 한국가축번식학회지
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• 제20권3호
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• pp.307-313
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• 1996

This study was conducted to investigate the effect of S-phase synthronized nuclear transfer on the development of nuclear transplant bovine embryos. A blastomere derived from the 16~32 cell stage bovine embryos was transferred into an enucleated metaphase II(MII) oocytes or activated S-phase eggs. From the MII-phase and S-phase nuclear transfer, 6.3%(4/63) and 13.8%(9/65) of nuclear transplant embryos developed to the blastocyst stage, respectively. In the S-phase nuclear transfer, maximal proportion of embryos developed to the blastocyst stage(16.6%) was obtained after the recipient cell was activated 8 h prior to receving a donor nucleus. MII-phase nuclear transplant embryos showed the PCC state of their nuclear at 1.5~2 h after fusion, whereas, S-phase nuclear transplant embryos did not undergo PCC. The result of this study suggests that if blastomeres of unknown cell-cycle-stage are used, S-phase nuclear transplantation through the activation of enucleated oocytes prior to fusion enhances development of nuclear transplant embryos. This result also suggests that the interval time from oocyte activation to cell fusion may affect the development of nuclear transplant embryos.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.6-6
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• 2002

This study was conducted to investigate the effect of recipient activation time on the nuclear remodeling, chromatin structure, pronuclear formation and in vitro development of bovine nuclear transfer embryos derived from adult ear skin cells. Somatic cells were transferred to enucleated oocytes after quiescent treatments by serum starvation or culture to confluency. Nuclear transfer embryos were activated with a combination of Ca/sup 2+/-ionophore and cycloheximide at 1, 1.5, 2, 2.5, 3, and 5 h after electrofusion. (omitted)

• 한국수정란이식학회지
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• 제9권2호
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• pp.161-166
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• 1994

This experiment was carried out to produce cloned aniraals by nuclear transplantation in rabbits. The ovulated oocytes were collected from the oviducts between 14 and 15 hours after hGG injection. The denuded oocytes were used as nuclear recipient cytoplasm following enucleation by micromanipulation. The blastomeres separated from the 8-cell embryos were used as nuclear donor. The nucleated oocytes receiving a blastomere in the perivitelline space were electrically fused in the 0.28 M mannitol solution at 1.5 kV /cm, 60$\mu$sec for three times. The nuclear transplant embryos which were used and developed to 2- to 4-cell stage in vitro were transferred into the oviducts of synchronized recipient does. A total of 64 nuclear transplant embryos were transferred to 7 recipient does and produced three offspring(4.7%) from a foster mother 31 days after embryo transfer.

• Nuclear Engineering and Technology
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• 제55권8호
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• pp.2997-3009
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• 2023

The Passive containment Air-cooling System (PAS) can effectively remove the decay heat of the modular small nuclear reactor after an accident. The details of natural convection around the dome, which is a key part of PAS, were investigated numerically in the present study. The thermal dynamics around the dome were studied through the temperature, pressure and velocity contours and the streamlines. Additionally, the formation of the buoyant plume at the top of the dome was investigated. The results show that with the increase of Ra, the lift-off point moves toward the bottom of the dome, and the eddy under the buoyant plume grows larger gradually, which enhances the heat transfer. And the heat transfer along the dome surface with different truncation angles was investigated. As the angle increases, the heat transfer coefficient becomes stronger as well. Consequently, a newly developed heat transfer correlation considering the influence of truncation angle for the dome is proposed based on the simulated results. This study could provide a better understanding of natural convection around the dome of PAS and the proposed correlation could also offer more predictive value in the improvement of nuclear safety.

• Nuclear Engineering and Technology
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• 제42권5호
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• pp.590-599
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• 2010

Recently, efficient operation and practical management of power plants have become important issues in the nuclear industry. In particular, typical aging parameters such as stress and cumulative usage factor should be determined accurately for continued operation of a nuclear power plant beyond design life. However, most of the major components have been designed via conservative codes based on a 2-D concept, which do not take into account exact boundary conditions and asymmetric geometries. The present paper aims to suggest an effective fatigue evaluation methodology that uses a prototype of the integrated model and its transfer functions. The validity of the integrated 3-D Finite Element (FE) model was proven by comparing the analysis results of individual FE models. Also, mechanical and thermal transfer functions, known as Green's functions, were developed for the integrated model with the standard step input. Finally, the stresses estimated from the transfer functions were compared with those obtained from detailed 3-D FE analyses results at critical locations of the major components. The usefulness of the proposed fatigue evaluation methodology can be maximized by combining it with an on-line monitoring system, and this combination, will enhance the continued operations of old nuclear power plants.

• Nuclear Engineering and Technology
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• 제49권7호
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• pp.1405-1413
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• 2017

To investigate the heat loads imposed on a reactor vessel through the natural convection of core melts in severe accidents, mass transfer experiments were performed based on the heat transfer/mass transfer analogy, using two- (2-D) and three-dimensional (3-D) facilities of various heights. The modified Rayleigh numbers ranged from $10^{12}$ to $10^{15}$, with a fixed Prandtl number of 2,014. The measured Nusselt numbers showed a trend similar to those of existing studies, but the absolute values showed discrepancies owing to the high Prandtl number of this system. The measured angle-dependent Nusselt numbers were analyzed for 2-D and 3-D geometries, and a multiplier was developed that enables the extrapolation of 2-D data into 3-D data. The definition of $Ra^{\prime}_H$ was specified for 2-D geometries, so that results could be extrapolated for 3-D geometries; also, heat transfer correlations were developed.

• 한국가축번식학회지
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• 제23권4호
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• pp.393-398
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• 1999

Somatic cell nuclear transfer is an efficient technique for the multiplication of elite livestock, engineering of transgenic animals, cell therapy and xenotransplantation, and analyzing the interactions between nucleus and cytoplasm, for various agricultural, biomedical and research purposes. Since the first somatic cell clone lamb was born, tremendous progress has been made toward developing technology for animal cloning. Viable farm animals and mice have now been produced by nuclear transfer using various fetal and adult somatic cells as nuclei donors. Transgenic clones were also produced from nuclear transfer of transfected somatic cells. In the future, somatic cell nuclear transfer will provide more numerous opportunities, both in basic and appled research as well as immediate uses in the generations of superior clone and transgenic animals. However, further technology refinement and improved understanding of the process are essential for commercial and basic research applications.

• 대한수의학회지
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• 제38권3호
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• pp.652-658
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• 1998

The efficiency of nuclear transfer using donor embryos originated from ovum pickup(OPU) and activated recipient cytoplasts were examined for induction of twinning in Korean native cattle(KNC). After aspiration of follicle by OPU, regardless of the vacuum applied, we obtained same result in proportions of recovered cumulus-oocyte complex (COCs) with compact cumulus. Under electric stimulation(1.0kV/cm DC for $40{\mu}s$), most of activated oocytes proceed to anaphase II/telophase II within 3h(84.7%). In the treatment of oocyte activation, the preactivation which was performed before fusion had significant effect on the developmental rates to morula/blastocyst stage(9.4 vs 4.0%). In embryo transfer of nuclear transferred embryos, we obtained 2 twins from KNC recipients and 1 twin from a Holstein recipient. Our results showed that it is possible to obtain twins using nuclear transfer technique in KNC.

• Nuclear Engineering and Technology
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• 제46권2호
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• pp.195-206
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• 2014

Heat transfer characteristics in a narrow rectangular channel are experimentally investigated for upward and downward flows. The experimental data obtained are compared with existing data and predictions by many correlations. Based on the observations, there are differences from others: (1) there are no different heat transfer characteristics between upward and downward flows, (2) most of the existing correlations under-estimate heat transfer characteristics, and (3) existing correlations do not predict the high heat transfer in the entrance region for a wide range of Re. In addition, there are a few heat transfer correlations applicable to narrow rectangular channels. Therefore, a new set of correlations is proposed with and without consideration of the entrance region. Without consideration of the entrance region, heat transfer characteristics are expressed as a function of Re and Pr for turbulent flows, and as a function of Gz for laminar flows. The correlation proposed for turbulent and laminar flows has errors of ${\pm}18.25$ and ${\pm}13.62%$, respectively. With consideration of the entrance region, the heat transfer characteristics are expressed as a function of Re, Pr, and $z^*$ for both laminar and turbulent flows. The correlation for turbulent and laminar flows has errors of ${\pm}19.5$ and ${\pm}22.0%$, respectively.