• 한국진공학회지
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• 제19권1호
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• pp.72-80
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• 2010

KSTAR ICRF 시스템에서 안테나 전류띠 간 커플링에 의한 전류띠의 전압, 전류 분포 변화와 전류띠 간 전력 전달에 의한 공명현상 그리고 전송선상의 이상 전압 분포 등을 예측하거나 분석하는 것은 그것의 안정적이고 신뢰성 있는 운전을 위해 매우 중요하다. 본 연구에서는 이러한 전류띠 간 커플링에 의한 현상들을 이해하기 위해 ICRF 안테나에서 측정한 S-parameter를 커플링이 고려된 8포트 전송선회로 모델에 적용하여 전류띠의 전송선 회로 모델을 완성하였다. 완성된 전송선 회로모델의 자체유도계수, 상호유도계수, 전기용량성 등은 전류띠의 유한한 길이로 인하여 2D 모델의 값보다 작은 것으로 나타났다. 커플링이 고려된 전류띠의 전송선 회로모델은 공명루프와 결합되어 있는 KSTAR ICRF 시스템의 운전에 활용될 것이다.

• Nuclear Engineering and Technology
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• 제55권11호
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• pp.4287-4294
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• 2023

In order to meet the energy demand, energy production must be done continuously. Hydrogen seems to be the best alternative for this energy production, because it is both an environmentally friendly and renewable energy source. In this study, the hydrogen fuel production of the peaceful nuclear explosives (PACER) fusion blanket as the energy source integrated with Fe-Cl thermochemical water splitting cycle have been investigated. Firstly, neutronic analyzes of the PACER fusion blanket were performed. Necessary neutronic studies were performed in the Monte Carlo calculation method. Molten salt fuel has been considered mole-fractions of heavy metal salt (ThF₄, UF₄ and ThF₄+UF₄) by 2, 6 and 12 mol. % with Flibe as the main constituent. Secondly, potential of the hydrogen fuel production as a result of the neutronic evaluations of the PACER fusion blanket integrated with Fe-Cl thermochemical cycle have been performed. In these calculations, tritium breeding (TBR), energy multiplication factor (M), thermal power ratio (1 - 𝜓), total thermal power (P_hpf) and mass flow rate of hydrogen (ṁ_H2) have been computed. As a results, the amount of the hydrogen production (ṁ_H2) have been obtained in the range of 232.24x10⁶ kg/year and 345.79 x10⁶ kg/year for the all mole-fractions of heavy metal salts using in the blanket.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.274-279
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• 2015

Receptor activator of nuclear factor-kappa B ligand (RANKL) is a critical factor in osteoclastogenesis. It makes osteoclasts differentiate and multinucleate in bone remodeling. In the present study, RANKL was expressed as a soluble maltose binding protein (MBP)-fusion protein using the Escherichia coli maltose binding domain tag system (pMAL) expression vector system. The host cell E. coli DH5α was cultured and induced by isopropyl β-_D-1-thiogalactopyranoside for rRANKL expression. Cells were disrupted by sonication to collect soluble MBP-fused rRANKL. The MBP-fusion rRANKL was purified with MBP Trap affinity chromatography and treated with Tobacco Etch Virus nuclear inclusion endopeptidase (TEV protease) to remove the MBP fusion protein. Dialysis was then carried out to remove binding maltose from the cleaved rRANKL solution. The cleaved rRANKL was purified with a second MBP Trap affinity chromatography to separate unsevered MBP-fusion rRANKL and cleaved MBP fusion protein. The purified rRANKL was shown to have biological activity by performing in vitro cell tests. In conclusion, biologically active rRANKL was successfully purified by a simple two-step chromatography purification process with one column.

• 한국응용곤충학회지
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• 제38권2호
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• pp.139-144
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• 1999

Autographa californica 핵다각체병 바이러스(AcNPV)의 다각체 단백질과 초록색 형광 단백질의 융합단백질의 특성을 분석하였다. 초록색 형광 단백질 유전자는 AcNPV의 완전한 다각체 단백질 유전자의 앞쪽과 뒤쪽에 융합하여 다각체 단백질 유전자의 프로모터 조절하에 도입하였다. 이렇게 작성된 재조합 바이러스를 각각 Ac-GFPPOL 또는 Ac-POLGFP이라고 명명하였다. 이들 재조합 바이러스에 의해 감염된 곤충세포주에서는 56kDa의 융합단백질이 발현되었다. 한편, 흥미롭게도 재조합 바이러스 Ac-POLGFP에 의해 감염된 세포주에서는 초록색 형광이 핵내에서만 다각체 유사 granular particle 형태로 관찰되었다. 반면에 Ac-GFPPOP에 의해 감염된 세포도주에서는 대부분 핵내에 존재하였지만, 세포질과 핵 모두에서 초록색 형광을 관찰할 수 있었다. 그러나 발현된 융합단백질은 분명히 다각체단백질을 포함하고 있음에도 다각체는 형성하지 않았다. 이러한 결과들은 융합단백질에서 다각체단백질의 위치와 관련이 있는 것으로 보여진다.

• Nuclear Engineering and Technology
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• 제29권4호
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• pp.348-359
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• 1997

This paper reviews cooling aspects of the diverter system in Tokamak fusion devices with primary emphasis on the critical heat flux (CHF) issues for oater-cooled designs. General characteristics of four (4) coolant options for diverter cooling gases, oater, liquid metal, and organic liquid - are discussed first, focusing on the comparison of advantages and disadvantages of those options. Then results of recent studies on the high-heat-flux CHF of water at subcooled high-velocity conditions are reviewed to provide a general idea on the feasibility of the water-cooled diverter concept for future Tokamak fusion reactors. Water is assessed to be the most viable and practical coolant option for diverters of future experimental Tokamaks.

• Nuclear Engineering and Technology
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• 제29권3호
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• pp.181-185
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• 1997

The ion sources for induction linac driven heavy ion fusion were fabricated and their omittance characteristics were investigated. For to kinds of ion sources, i. e. a carbon vacuum arc ion source and a cusp field rf ion source, the emittance was measured with a double slit beam scanner. The required normalized omittance of an ion source for heavy ion fusion is 10$^{-7}$ - 5$\times$10$^{-7}$ $\pi$ m-rod, and the measured emittances of the ion beams from carbon vacuum arc ion source and cusp field rf ion source (Ne$^{+}$) were 2$\times$10$^{-6}$ $\pi$ m-rad and 4$\times$10$^{-7}$ $\pi$ m-rad, respectively.y.

• Nuclear Engineering and Technology
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• 제3권1호
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• pp.33-36
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• 1971

액체에 대한 Significant Structure 이론을 토대로 하여 융해의 기본적 원리를 성공적으로 논의하였다. 융해의 이론을 실증하는 보기로서 융점에서의 부지의 팽창을 용융염등 몇가지 액체에 대해 계산하였다. 계산결과는 실험치와 이론치 사이에 좋은 일치를 보여주고 있다. 용융염의 연구는 원 자료에 쓰는 고온도 액체의 성질과 구조를 이해하는데 도움을 줄 것이 다.

• Nuclear Engineering and Technology
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• 제53권9호
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• pp.2899-2908
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• 2021

The laser transport system of the high power laser facility is mainly composed of large-aperture laser transport mirrors (TMs). Obtaining the high-resolution online damage images during the operation, which is of great significance for operating safely of the mirrors and the facility. Based on wavefront coding, pan-tilt scanning and image stitching technologies, an online laser-damage images detection system is designed, and it can achieve high-precision detection of surface characteristics of large-aperture laser transport mirrors. The preliminary simulation proves that the system can solve the depth of field matching problem caused by pan-tilt tilt imaging and achieve higher resolution.

• 대한금속재료학회지
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• 제49권8호
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• pp.589-595
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• 2011

Laminated glass fiber-reinforced plastic (GFRP) composites were applied to an insulating structure of a magnet system for a nuclear fusion device. Decreased inter-laminar strength by a strong repulsive force between coils which is induced a problem of structural integrity in laminated GFRPs. Therefore, it is important to investigate the inter-laminar characteristics of laminated GFRP composites in order to assure more reliable design and better structural integrity. Three types of the laminated GFRP composites using a high voltage insulating materials were fabricated according to each molding process. To evaluate the grade of the fabricated composites, mechanical tests, such as hardness, tensile and compressive tests,were carried out. The autoclave molding composites satisfied almost of the mechanical properties reguested at the G10 class standard, but the vacuum impregnation (VPI) and Prepreg composites did not.

• BMB Reports
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• 제44권8호
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• pp.529-534
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• 2011

Ribosomal protein S3 (rpS3) is a multifunctional protein involved in translation, DNA repair, and apoptosis. The relationship between rpS3 and cyclin-dependent kinases (Cdks) involved in cell cycle regulation is not yet known. Here, we show that rpS3 is phosphorylated by Cdk1 in G2/M phase. Co-immunoprecipitation and GST pull-down assays revealed that Cdk1 interacted with rpS3. An in vitro kinase assay showed that Cdk1 phosphorylated rpS3 protein. Phosphorylation of rpS3 increased in nocodazole-arrested mitotic cells; however, treatment with Cdk1 inhibitor or Cdk1 siRNA significantly attenuated this phosphorylation event. The phosphorylation of a mutant form of rpS3, T221A, was significantly reduced compared with wild-type rpS3. Decreased phosphorylation and nuclear accumulation of T221A was much more pronounced in G2/M phase. These results suggest that the phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase and, moreover, that this event is important for nuclear accumulation of rpS3.