• Journal of Periodontal and Implant Science
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• 제43권6호
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• pp.269-275
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• 2013

Purpose: In this study, the effect of calcium sodium phosphosilicate (NovaMin) desensitizing agent, which is a powder-based system, and hydroxyethyl methacrylate and glutaraldehyde (Gluma desensitizer), which is liquid-based system, on dentinal tubule occlusion was analyzed by scanning electron microscope. The effects of the above two along with one control group were compared to determine the more effective method of sealing the dentinal tubules after initial application. Methods: Twenty specimens were allocated to each of 3 groups: Control, Gluma desensitizer, and NovaMin. Two additional samples were also prepared and treated with Gluma and NovaMin; these samples were longitudinally fractured. The specimens were prepared from extracted sound human premolars and were stored in 10% formalin at room temperature. The teeth were cleaned of gross debris and then sectioned to provide one to two dentin specimens. The dentin specimens were etched with 6% citric acid for 2 minutes and rinsed in distilled water. Control discs were dried, and the test discs were treated with the desensitizing agents as per the manufacturer's instructions. The discs as well as longitudinal sections were later analyzed under the scanning electron microscope. The proportions of completely occluded, partially occluded, and open tubules within each group were calculated. The ratios of completely and partially occluded tubules to the total tubules for all the groups was determined, and the data was statistically analyzed using nonparametric tests and statistical significance was calculated. Results: NovaMin showed more completely occluded tubules ($0.545{\pm}0.051$) while Gluma desensitizer showed more partially occluded tubules ($0.532{\pm}0.075$). The differences among all the groups were statistically significant ($P{\leq}0.05$). Conclusion: Both materials were effective in occluding dentinal tubules but NovaMin appeared more promising in occluding tubules completely after initial application.

• Archives of Pharmacal Research
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• 제25권5호
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• pp.613-616
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• 2002

A high-performance liquid chromatographic method has been developed for the separation and quantification of chrysin and synthetic chrysin derivatives (12 chrysin alkyl and 7 chrysin acyl derivatives). The chromatography was performed using a $Nova-Pak^{\circledR}C_{18}$ column. A RP-HPLC was performed by using a binary mixture (MeOH-10 mM H$_3$PO$_4$) as a mobile phase, and the column temperature was maintained at room temperature. A flow rate was 1.0 ml/min, and the effluent was monitored at a wavelenth of 280 nm. The retention times for chrysin acyl and alkyl derivatives were within 10 minutes and 20 minutes, respectively. The absolute recovery of samples were all over 96%. The detection limits were 0.1~18 ng at S/N = 3 ratio.

• 원예과학기술지
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• 제29권6호
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• pp.561-567
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• 2011

상록활엽관목으로서 꽃이 화려하고 관상가치가 높으나 멸종위기 수종인 자생만병초와 원예도입종 만병초 품종을 공시하여 내한성의 기작을 잎 운동과 광합성 활성 등의 생리적 반응을 조사하여 구명하고자 하였으며 다음과 같이 요약된다. 월동 후의 엽소 피해 정도로 측정한 내한성 정도는 자생종인 만병초와 홍만병초에서 도입종보다 크게 높았으며, 도입종 중에서도 'Nova Zembla'에서 가장 낮았고, 'Cynosure'와 'Parker's Pink'는 이보다 다소 높았다. 저온에 의한 엽각의 변화는 자생종과 'Parker's Pink' 품종에서 크게 일어났으며, 'Nova Zembla'와 'Cunningham's White'에서의 변화는 적었다. 저온에 의한 잎말림 현상도 자생종에서 크게 일어났고 도입종은 이들보다 작았으며, 도입종 중에서는 'Parker's Pink'가 다소 컸고 나머지 품종들은 작았다. 자생종의 내한성은 저온에 의한 엽각변화와 잎말림현상이 관여하는 것으로 판단되며, 내한성이 약한 'Parker's Pink'에 있어서는 잎운동 활성과의 관련성이 적어 다른 요인이 관여하고 있는 것으로 생각된다. 내한성이 강한 만병초에서 월동 전 광합성 활성이 높았고 월동 후 광합성활성의 회복이 자생종 및 도입종의 'Cynosure'에서 비교적 빨랐으며, 내한성이 가장 약했던 'Nova Zembla'에서 월동 전 광합성 활성과 월동 후 광합성활성의 회복속도가 가장 낮았다. 기공전도도는 광합성활성과 유사한 경향을 보였으며, 내한성이 강한 자생종에서 월동 후 뿌리로부터 잎으로의 수분이동이 원활하여 엽소현상이 적었고, 월동 후 기공전도도가 가장 낮았고 수분이동에 크게 제한을 받은 'Nova Zembla'에서 엽소현상이 크게 나타난 것으로 생각된다. 이상의 결과로부터 만병초 종에 있어서 월동중의 잎 운동과 월동후의 수분이동 및 광합성에 의해 축적되는 수분스트레스 관련 물질들이 종합적으로 관여하여 엽소현상으로서 나타나는 내한성을 결정하는 것으로 생각된다.

• 한국응용곤충학회지
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• 제46권2호
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• pp.201-212
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• 2007

한국산 Tipula속에 속하는 Tipula latemarginata Alexander (애아이노각다귀), T. nova Walker (애잠자리각다귀), T. aino Alexander (아이노각다귀)3 종의 생태를 조사하기 위하여 2001년 1월부터 2005년 12월까지 봉화군 내성천 유역의 3개 지점에서 야외 조사와 함께 실험실에서 사육 실험을 병행하였다. 유충은 계류, 하천, 논, 습한 땅 또는 물이 새어나오는 옹벽 등에서 서식했으며, 부화 직후의 1령기 유충은 물 속에서 연한 조류 (algae)를 섭식하나 성장하면서 여러 종류의 식물의 잎, 줄기, 뿌리, 딱딱한 낙엽 조각 등을 먹는 초식성으로 조사되었다. 탈피 시 유충은 몸을 좌우상하로 계속 움직이며, 탈피에 소요되는 시간은 2시간 이내였고, 탈피 후 4시간 정도 지나면서 왕성한 섭식 활동을 보였다. 유충은 용화 시기가 되면 물에서 흙으로 상륙하여 용이 되었으며, 4령기에서 $1{\sim}2$ 일 이내의 짧은 전용 단계를 거쳤다. 용은 우화 시, 두흉부는 흙 위로 내 놓고 몸 아래 부분은 흙 속에 넣은 상태로 우화하였으며, 용소 (pupal case) 에서 성충으로 우화되는데 약 20 분에서 1시간 정도 걸렸다. 우화 후 약 $5{\sim}7$분 후에 교미를 하였으며, 약 40 분 정도 교미 상태가 지속되었다. 암컷 성충은 물기가 많은 질퍽한 흙이나, 조류 충(algal bed) 위에 산란하였으며, 몸통은 지면에 수직으로, 날개는 $120^{\circ}$각도로 편 상태에서 3 쌍의 다리로 지면 위를 걸어 다니면서 산란했다. 산란은 보통 우화 후 $1{\sim}4$일 사이에 일어났으며, 암컷 성충 1개체 당 평균 $501{\sim}760$ 의 알을 보유하는 것으로 조사되었다. 성충은 일반적으로 먹이를 먹지 않고 가끔 불만 먹었으며, 사육한 개체의 경우, 우화 후 처음에는 물을 잘 먹지 않았으나, 수명이 다 되어갈수록 물을 자주 먹는 것으로 나타났다. 성충은 여름철에 물만 주고 사육하면, 그 수명이 $4{\sim}9$ 일 정도였으며, 수컷의 수명이 암컷보다 조금 더 길었다. T. nova는 3% 설탕물로 사육하면 물로 사육한 경우보다 수명이 3배 이상 긴 것으로 조사되었다. 성비는 T. latemarginata의 경우, 야생 상태에서 수컷 대 암컷의 비가 2.6 : 1 로 수컷이 훨씬 많았다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.389.1-389.1
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• 2002

A high-performance liquid chromatographic method was developed to determine the quantities of curcumin and glycyrrhizin in two different oriental herbal preparations of Kamijadowhan (KMD. NKMD). Two compounds were separated in less than 10 min with a Nova-Pak $C_{18}$ column ($3.9{\times}150$ mm ($5{\mu}$ particle size) by linear gradient elution using 0.03% (v/v) phosphoric acid-acetonitrile (60:40. v/v% at 0 min; 40:60 v/v% at 6 mini as the mobile phase at a flow-rate of 0.8 ml $min^{-1}$. (omitted)

• 대기
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• 제26권4호
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• pp.617-633
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• 2016

Jangbogo station is located in Terra Nova Bay over the East Antarctica, which is often affected by individual storms moving along nearby storm tracks and a katabatic flow from the continental interior towards the coast. A numerical simulation for two strong wind events of maximum instantaneous wind speed ($41.17m\;s^{-1}$) and daily mean wind speed ($23.92m\;s^{-1}$) at Jangbogo station are conducted using the polar-optimized version of Weather Research and Forecasting model (Polar WRF). Verifying model results from 3 km grid resolution simulation against AWS observation at Jangbogo station, the case of maximum instantaneous wind speed is relatively simulated well with high skill in wind with a bias of $-3.3m\;s^{-1}$ and standard deviation of $5.4m\;s^{-1}$. The case of maximum daily mean wind speed showed comparatively lower accuracy for the simulation of wind speed with a bias of -7.0 m/s and standard deviation of $8.6m\;s^{-1}$. From the analysis, it is revealed that the each case has different origins for strong wind. The highest maximum instantaneous wind case is caused by the approach of the strong synoptic low pressure system moving toward Terra Nova Bay from North and the other daily wind maximum speed case is mainly caused by the katabatic flow from the interiors of Terra Nova Bay towards the coast. Our evaluation suggests that the Polar WRF can be used as a useful dynamic downscaling tool for the simulation and investigation of high wind events at Jangbogo station. However, additional efforts in utilizing the high resolution terrain is required to reduce the simulation error of high wind mainly caused by katabatic flow, which is received a lot of influence of the surrounding terrain.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집 Vol.14 No.1
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• pp.176-180
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• 2001

There are several factors causing re-work in CMP process such as improper polish time calculation by operator. removal rate decline of the polisher, unstable in-suit pad conditioning, slurry supply module problem and wafer carrier rotation inconsistancy. And conclusively those fundimental reason for the re-work rate increasement is mainly from the cycle time delay between wafer polish and post measurement. Therefore, Wafer thickness measurement in wet condition could be able to remove those improper process conditions which may happen during the process in comparison with the conventional dried wafer measurement system and it can be able to reduce the CMP process cycle time. CMP scrap reduction by overpolish, re-work rate reduction, thickness control efficiency also can be easily achieved. CMP Equipment manufacturer also trying to develop integrated system which has multi-head & platen, cleaner, pre & post thickness measure and even control the polish time from the calculated removal rate of each polishing head by software. CMP re-work problem such as over & under polish by target thickness may result in the cycle time delay. By reducing those inefficient factors during the process and establish of the automatic process control, CLC system need to be adopted to maximize the process performance. Wafer to Wafer Polish Time Feed Back Control by measuring the wafer right after the polish shorten the polish time calculation for the next wafer and it lead to the perfact Post CMP target thickness control capability. By Monitoring all of the processed the wafer, CMP process will also be stabilize itself.

• 한국식품영양과학회지
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• 제22권6호
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• pp.815-822
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• 1993

This experiment was carried out to determine the separation condition of 1-dimethylaminonaphthalene-5-sulfony(Dansyl) derivatives of amino acids by reverse-phase high performance liquid chromatography with Nova-Pak C18 column. Determined solvent system was solvent A(200mA phosphate buffer pH 6.8 15%, acetonitrile 11%, water 74%) and solvent B(acetonitrile 65%, methanol 28%, water 7%). Linear gradient of solvent B was applied from 12% to 80% for 50min. Complete separation of 20 amino acids including asparagine and glutamine which constitute protein was achieved within 50min. As the detection limit was the range of picomole, the resolution power was excellent. Reproducibility of the retention time was less than mean $\pm$0.05min. According to the above optimum chromatographic conditions, the amino acid composition of some food and human blood was examined. The most affluent amino acid was alanine in human blood, aspartic acid and glutamic acid in soy sauce, alanine and threonine in soy milk and proline in milk and yoghurt.

• 대한수의학회지
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• 제43권3호
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• pp.429-433
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• 2003

This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the Nova-Pak $C_{18}$ reverse phase column. The mobile phase consisted of 0.1% acetic add in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.

• 분석과학
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• 제11권5호
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• pp.404-408
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• 1998

Using a reversed-phase high performance liquid chromatography, the separation of 20(S)-, 20(R)-prosapogenin stereo-isomers of ginsenoside-$Rg_2$ and of ginsenoside-$Rg_3$ in ginseng saponins has been carried out with binary solvent system. The optimum conditions for the isomer separation are as following: Nova-$Pak^{(R)}C_{18}$ (Waters, $3.9{\times}150mm$) column, $CH_3CN/CH_3CN$ (100:8, v/v) binary solvent system and the flow rate was 1.7 mL/min. The stereoisomers were separated with change of the mixture ratio of the solvent system, the solvent elution by gradient program, and then detected at 203 nm of UV detector. The simultaneous separation of mixture that were the $Rg_2$, $Rg_3$ isomers was easily performed in nonpolar solvent for $Rg_2$, polar solvent for $Rg_3$ at the same optimum conditions.