• Journal of Veterinary Science
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• 제21권1호
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• pp.9.1-9.15
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• 2020

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

• Animal Bioscience
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• 제35권5호
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• pp.698-710
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• 2022

Objective: The objective of this study was to investigate the effects of different degrees of maternal dietary energy restriction on lipid deposition in embryonic tissues during the medium laying period (37 to 39 weeks) in Arbor Acres (AA) broiler breeders. Methods: A single factor design was adopted, and 400 AA broiler breeders (20 weeks of age) with a similar weight were randomly allocated into four groups. The birds in the control group were fed a corn-soybean meal based diet, and those in trial groups were fed diets with 80%, 70%, and 50% energy levels of the basal diet. Incubated eggs from the medium laying period were collected. Samples of developing embryos at various stages were prepared for composition analysis. Results: The embryo weight in the 80% energy group was higher than those of the other groups on embryonic day (E) 13, but at 21 E, they were significantly decreased with decreasing energy intake of the broiler breeders (p<0.05). Additionally, the levels of crude fat in tissues in the restriction groups were significantly decreased (p<0.05). The long axis and area of adipocytes in breast muscle, thigh muscle and the liver were significantly decreased (p<0.05) at 21 E in the 80%, 70%, and 50% energy groups. Conclusion: The effects of the 80% maternal dietary energy restriction energy affects egg production performance, egg quality, and nutrient deposition in egg weights, which then directly impacts on the developmental process of embryos, especially on fat utilization and deposition.

• Bulletin of the Korean Chemical Society
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• 제27권10호
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• pp.1677-1680
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• 2006

• Bulletin of the Korean Chemical Society
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• 제29권2호
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• pp.511-514
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• 2008

• 한국산림과학회지
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• 제97권5호
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• pp.501-507
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• 2008

The data of crown width with 4 directions, DBH, tree height, and coordinate for sample trees were collected from 30 permanent sample plots in secondary fore st of the Maoershan Experimental Forestry Farm, Northeast China. In this paper, the competition of individual trees in stand were discussed for secondary forest by using iterative Hegyi competition index and crown overlap index that represented the competitive and cooperative interactions among neighboring trees. Active competitors of subject tree in the competition zone were selected to calculate the iterative competition index. Using the results of crown classification based on the equal crown projection area, a new distance dependent competition index called crown overlap index (COI) was developed for secondary forest. The COI performed well in describing the crown competition rather than crown competition factor (CCF). The individual-based competition index discussed in this paper will provide more precise for developing individual tree growth models for secondary forest and it can also use to adjust the stand structure for spatial optimal management.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2006년도 학술발표회 논문집
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• pp.18-21
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• 2006

Though there are several definitions for the Northeast Asia, in this paper Northeast Asia is defined as the geographical sphere including five countries. Those five countries are China, the Democratic People's Republic of Korea, Japan, Mongolia and the Republic of Korea. For the announcement of regional preparatory process at the $4^{th}$ World Water Forum (4WWF), basic characteristics and main water-related issues were surveyed in the Northeast Asian countries. This paper is mainly composed of basic characteristics of each country, main water challenges, implemented strategies, successful experiences and local actions, etc. The five countries provided one's country report on the Government basis in 2004 to Korea Water Forum (KWF) as the coordinator of Northeast Asia for the 4WWF. KWF summarized the report of each country very briefly as follows.

• 무역상무연구
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• 제33권
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• pp.277-308
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• 2007

Northeast Asia Nations introduced each single window system for simplifying the customs clearance procedures in order to prevent the rekeying the customs and trade-related data. A single window is a means for enabling users to complete all the necessary import/export procedures in a single input and by single transmission by linking the related system. WCO recommends CDM, UCR, ACI that completed by WCO's own projects for assist to establish the single window for customs clearance. Northeast Asia Nations such as Korea, China, Taiwan, Japan undertook trade process reengineering for introducing single window for customs clearance. Korea and Japan introduced a e-customs systems as a good single window system. The purpose of this paper is to contribute to construction of the efficient single window system for customs clearance by studying e-customs cases in Northeast Asia Nations.

• 통상정보연구
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• 제8권3호
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• pp.167-189
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• 2006

In this year(2006), the Korean government has announced the integrated logistics promotion act, to improve the international competitiveness of Korean logistics companies and to develop the north-east asia logistics hubs. Therefore, in this study, emphasized to goes forward better and presents integrated logistics industry expansive assignment through domestic logistics companies' problem and integrated logistics industry investigation of certification system as plan for successful fixing of integrated logistics industry for Northeast Asia logistics hub while strengthen international competitiveness of domestic professional logistics companies. So that the South Korea becomes Northeast Asia logistics hub country, to expand lacking logistics infra at good time is important, also, reform backward logistics system of consignor companies and advance actively to the Northeast Asia logistics market is very important to improve the professional logistics companies' upbringing to lead the Northeast Asia logistics market.

• 통상정보연구
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• 제11권2호
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• pp.127-145
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• 2009

With increase in oil demand owing to economic growth in Northeast Asia, the establishment of an oil hub in Korea is recently considered by policy and academic circles. This paper aims to suggest strategic schemes on the establishment of Korea's oil hub in Northeast Asia by comparing Korea's conditions as an oil logistics hub with Singapore which has been an oil hub in East Asia until recent year. Quantitative and qualitative analysis methods were employed in order to examine the competitiveness of Korea's oil hub. This research concludes that Korea is very competitive as an oil logistics hub in Northeast Asia, but so much improvements for realizing the project are necessary such as expansion of storage facilities for oil, institutional instruments for facilitating oil logistics and trading and preparation for incentive schemes on foreign investments in oil and its related industries.

• Journal of Forest and Environmental Science
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• 제28권2호
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• pp.68-74
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• 2012

A total of 45 Pinus sylvestnis var. mongolica trees from 9 plots in northeast China were destructively sampled to develop aboveground prediction equations for inventory application. Sampling plots covered a range of stand ages (12-47-years-old) and densities (450-3,840/ha). The distribution of aboveground biomass of whole-trees and tree component (stems, branches and leaves) of individual trees were studied and 4 equations were developed as functions of diameter at breast height (DBH), total height (HT). All the equations have good estimation effect with high prediction precision over 90%. Forest biomass was estimated based on the individual biomass prediction equations. It was found forest biomass of all organs increased with the increasing of stand age and density. And the period of 45-50 years was the suitable harvest time for Pinus sylvesris plantation.