• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.38-38
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• 2003

Blocking the first mitotic cleavage of the zygote is a key tool for chromosome-set manipulations in fish. We developed an improved method for inducing tetraploidy by blocking the mitosis with a combination of heat shock at 40.5$^{\circ}C$ for 1, 2 or 3 min followed by cold shock at $1.5^{\circ}C$ for 30, 45 or 60 min. When applied during the first cleavage metaphase of mud loach (Misgurnus mizolepis) zygotes, the optimal combination was heat for 2 min followed by cold for 45 min. At 1 month, the frequency of 4N survivors and the yield from total eggs fertilized was 55.7% and 14.4%, respectively, compared to heat shock alone with 20.0% efficiency and 3.6% yield. The effectiveness of the procedure was confirmed by diploid mitotic gynogenesis using transgenic markers. The overall yield of homozygous diploids, 34.0%, was better than that for single heat shock, 17.3%. The tetraploids and homozygous diploids had higher early mortality than normal diploid controls. However at 1 month, the viability of the tetraploids was the same as normal diploids. For gynogenetic diploids, the survival was similar to normal diploids after 3 months. The high efficiency of this new protocol extends the opportunity to study polyploidy in basic and applied research.

• Journal of environmental and Sanitary engineering
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• v.13 no.2
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• pp.47-56
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• 1998

Effects of sulfate on the anaerobic substrate utilization were evaluated using UASB (Upflow Anaerobic Sludge Blanket) reactor. Effect of sulfate on the organic removal rate was dependent on the relative amount of microorganisms in the reactor, the operational condition, and the characteristics of sludge. When the sulfate shock was applied to 0.0 - 3.0g SO$_{4}$$^{2-}$/d, more than 95% of COD removal efficiency was achieved. Therefore, if F/M ratio was kept to low sufficiently with recirculation, it is shown that operation of the reactor was not affected significantly, though sulfate shock load was doubled compared to the normal operation. Provided that it is shocked by high strength of sulfate or temporary shock load is applied frequently the efficiency of reactor may be disadvantageous as well as the wash-out of sludge will be increased by decreasing the size to the accumulated frequency of granular sludge and the size with maximum frequency.

• Journal of the Korean Society of Propulsion Engineers
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• v.17 no.4
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• pp.63-72
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• 2013

A Mach 5 nozzle and a diffuser of the Scramjet Engine Test Facility (SETF) were made for a hydrocarbon-fueled scramjet engine. SETF, attached with a diffuser guide, started with a model of 60% blockage, though the model engine could not start by over expansion of the facility nozzle. The model was moved into the nozzle to escape the shock generated from the nozzle exit, both SETF and the engine could start. The pitot rake experiments (blockage of 2.3%) were done for measuring the core flow in the test section. From the pitot experiments, the core flow was expanded by an under expansion. It means that the core flow in the test section was related with a model blockage. SETF and the engine with a blockage of 33% work normally. From a series of experiments, SETF started with a normal shock efficiency of 58%, regardless of a blockage ratio.

• Mass Spectrometry Letters
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• v.7 no.1
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• pp.1-11
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• 2016

Various efforts have been developed to improve sample preparation steps, which strongly depend on hands-on processes for accurate and sensitive quantitative proteome analysis. In this study, we carried out heating the sample prior to trypsin digestion using an instrument to improve the tryptic digestion process. The heat shock generated by the system efficiently denatured proteins in the sample and increased the reproducibility in quantitative proteomics based on peptide abundance measurements. To demonstrate the effectiveness of the protocol, three cell lines (A human lung cancer cell line (A549), a human embryonic kidney cell line (HEK293T), and a human colorectal cancer cell line (HCT-116)) were selected and the effect of heat shock was compared to that of normal tryptic digestion processes. The tryptic digests were desalted and analysed by LC-MS/MS, the results showed 57 and 36% increase in the number of identified unique peptides and proteins, respectively, than conventional digestion. Heat shock treated samples showed higher numbers of shorter peptides and peptides with low inter-sample variation among triplicate runs. Quantitative LC-MS/MS analysis of heat shock treated sample yielded peptides with smaller relative error percentage for the triplicate run when the peak areas were compared. Exposure of heat-shock to proteomic samples prior to proteolysis in conventional digestion process can increase the digestion efficiency of trypsin resulting in production of increased number of peptides eventually leading to higher proteome coverage.

• Journal of the Korean Society of Propulsion Engineers
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• v.12 no.2
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• pp.24-32
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• 2008

A non-reacting experimental study on a normal injection into a Mach 1.92 crossflow which flows over various geometries(flat plate, small cavity, large cavity) was carried out to investigate the effect of the momentum flux ratio(J). The aft ramp of the cavity advances the increase of the penetration height and the strong two-dimensional shock from recompression region mainly affects the shock structure and mixing layer at the downstream flow. As flow runs downward, the transverse penetration height increases with increasing J(J = 0.9, 1.7, 3.4). However, above some critical ratio, jet penetration height growth with increasing J is not appeared in flow-field. Large scale cavity has a good mixing efficiency but it increases the drag loss in the combustor.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.1664_1665
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• 2009

Many years ago, most of thermal power plants built in this country were of subcritical pressure, of medium or small size, of constant pressure operations and of drum type steam generators with circulation type boilers. But, nowadays almost all of them were of high efficiency, of supercritical pressure, of big capacity, of sliding pressure operations, and of once through type steam generator. It has such advantage as the reduction of startup duration, but it control system and operation method are very complicated. It has a big difference in operation method of turbine and boiler. The feedforward control needs to be introduced to prevent such problems as thermal shock during the transit from normal operation into bypass operation. This paper introduces the turbine control and turbine bypass control during startup of thermal power plants.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.45 no.11
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• pp.914-921
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• 2017

The experimental research on a high-altitude environment simulation of space launch vehicle is important for securing independent technologies with launching space vehicles and completing missions. This study selected an altitude of 65 km for the experiment environment where it exceeded Mach number of 6 after the launch of Korean Space Launch Vehicle(KSLV-II). Shock tunnel was used to replicate the flight condition. After flow establishment, in order to confirm aerodynamic characteristics and normal and oblique shockwaves, the flow verification was carried out by measuring stagnation pressure and heat flux of a forebody model, and shockwave stand-off distance of a hemispherical model. In addition, a shock-free technique to recover free-stream condition has been developed and verified. From the results of the three verification tests, it was confirmed that the flow was replicated with the error of about ${\pm}3%$. The error between the slope angle of inclined shockwave of the scaled down transition section model using the shock-free shape and the slope angle of the horizontal plate model, and between the theoretical and the experimental value of the static pressure of the model were confirmed to be 2% and 1%, respectively. As a result, the efficiency of the shockwave cancellation technique has been verified.

• Current Research on Agriculture and Life Sciences
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• v.17
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• pp.39-43
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• 1999

To investigate the function of chloroplast-localized small HSP in rice, the cDNA, Oshsp21, was introduced into rice plants via Agrobacterium-mediated gene transfer system. Calli induced from rice immature embryos were co-cultivated with a A. tumafaciens EHA101 that contained a plasmid, pIHSP21. The efficiency of plant regeneration from the calli co-cultivated with the Agrobacterium was about 30%. PCR and Southern blot analyses using genomic DNA revealed that gene for the chloroplast small HSP was introduced into the genome of rice. Expression of transgene was investigated by northern blot analysis. Results indicate that the transgene, Oshsp21, was constitutively expressed at normal growth temperature.

• Journal of the Korean Society of Propulsion Engineers
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• v.19 no.6
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• pp.81-90
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• 2015

In order to know the characteristics of the hypersonic air-breahting engine, high altitude and Mach number ground test is necessary. Therefore, high pressure and high temperature condition should be simulated to do ground test of the hypersonic air-breathing engine. In this paper, the hypersonic air-breathing engine ground test facility of the Korea Aerospace Research Institute was introduced and the composition and characteristics were described.

• Journal of Advanced Marine Engineering and Technology
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• v.10 no.3
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• pp.94-106
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• 1986

Since the oil shock of '70s the engine makers have developed new types of diesel engine with low fuel consumption. There is an obvious tendency towards the use of poorer quality fuels, such as the residual oil from chemical processes of refinery. The shaft driving generators is also widely adopted on behalf of the auxiliary diesel engines, which are driving on the expensive diesel oil and have high fuel oil consumption rates, and some mania propulsion diesel engines are equipped with reduction gear systems to get better propulsive efficiency by slower propeller revolutions. The propulsion shafting system equipped with the shaft driving generator or the geared diesel engine shafting system has flexible couplings, and it requires extensive investigations of the torsional vibration and torque fluctuation in order to ensure the acceptable operation range in service. The characteristics of misfiring must be especially examined for the high viscosity fuels to be used. Both torsional vibration and fluctuating torque resulted from misfiring, should be examined for thier effects on the flexible coupling and propulsion shafting system. This paper is to investigate and solve the above mentioned problems which must be predicted on the design-stage of marine propulsion shafting system. A computer program is developed to calculate the indicated diagram, fluctating torque and torsional vibration for both normal and misfiring conditions.