• Journal of KSNVE
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• 제9권1호
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• pp.113-120
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• 1999

A perforated tube nozzle as an exhaust noise suppressor of a high-speed civil transport(HSCT) is proposed. The experimental results for the near and far field sound. the visualization of jet structures and the static pressure distributions in the jet passing through a perforated tube are presented and discussed in comparison with those for a simple tube. It is shown that the perforated tube has an excellent performance to greatly reduce the shock-associated noise and that also the turbulent mixing noise is reduced in the range of a limited jet pressure ratio. This considerable noise reduction is due to the pressure relief caused by the through-flow through the perforated holes. Such a pressure relief results in the transformation of normal shock waves into weak Mach waves of X -type and increases the thrust force of the perforated tube nozzle.

• BMB Reports
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• 제38권6호
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• pp.695-702
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• 2005

The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichia coli ${\sigma}^{32}$-type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SS1 approximately 10min after increasing the temperature from 30 to $42^{\circ}C$. The groESL operon was also induced by hydrogen peroxide or salt shock.

• Advances in materials Research
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• 제6권2호
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• pp.155-168
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• 2017

The terrorist attacks and dangers by bomb blast have turned into an emerging issue throughout the world and the protection of the people and structures against terrorist acts depends on the prediction of the response of structures under blast and shock load. In this paper, behavior of aluminum and unidirectionally reinforced E-Glass Epoxy composite plates with and without focal circular holes subjected to shock loading has been identified. For isotropic and orthotropic plates (with and without holes) the classical normal mode approach has been utilized as a part of the processing of theoretical results. To obtain the accurate results, convergence of the results was considered and a number of modes were selected for plate with and without hole individually. Using a shock tube as a loading device, tests have been conducted to composite plates to verify the theoretical results. Moreover, peak dynamic strains, investigated by experiments are also compared with the theoretical values and deviation of the results are discussed accordingly. The strain-time histories are likewise indicated for a specific gauge area for aluminum and composite plates. Comparison of dynamic-amplification factors between the isotropic and the orthotropic plates with and without hole has been discussed.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• 제12권7호
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• pp.510-519
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• 2002

The present study addresses a computational work of the weak shock wave propagating inside an automobile exhaust muffler. Several different types of the silencer systems are employed to investigate the magnitude of the shock wave during propagating through them. The Initial shock wave Mach number $M_s$ is varied between 1.01 and 1.30, and a normal shock wave is given at the inlet of the silencer systems. The second order total variation diminishing scheme Is employed to solve the two dimensional, compressible, unsteady Euler equations. The present computational results are compared with the previous experimental ones available. The present computations predict the experimental results with a quite good accuracy. Of the four silencer systems applied. the most desirable silencer system to reduce the peak pressure at the exalt of the exhaust pipe is discussed from the Point of view of the engineering design of the silencer systems.

• Journal of Embryo Transfer
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• 제33권2호
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• pp.75-84
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• 2018

The cryopreservation has been extensively applied in many cells including spermatozoa (semen) during past several decades. Especially, the canine spermatozoa cryopreservation has contributed on generation of progeny of rare/genetically valuable dog breeds, genome resource banking and transportation of male germplasm at a distant place. However, severe and irreversible damages to the spermatozoa during cryopreservation procedures such as the thermal shock (cold shock), formation of intracellular ice crystals, osmotic shock, stress of cryoprotectants and generator of reactive oxygen species (ROS) have been addressed. According as a number of researches have been conducted to overcome these problems and to advance cryopreservation technique, several analytical methods have been employed to evaluate the quality of the fresh or cryopreserved canine spermatozoa in regards to the motility, morphology, integrity of membrane and DNA, mitochondrial activity, ROS generation, binding affinity to oocytes, in vitro fertilization potential and fertility potential by artificial insemination. Because the study designs with certain application of analytical methods are selective and varied depending on each experimental objective and laboratory condition, it is necessary to establish the normal reference data of the fresh or cryopreserved canine spermatozoa for each analytical method to monitor experimental procedure, to translate raw data and to discuss results. Here, we reviewed the recent articles to introduce various analytical methods for the canine spermatozoa as well as to establish the normal reference data for each analytical method in the fresh or cryopreserved canine spermatozoa, based on the results of the previous articles. We hope that this review contributes to the advancement of cryobiology in canine spermatozoa.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• 제17권2호
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• pp.167-181
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• 2019

Currently, the development of evaluation technology for vibration and shock load characteristics and spent nuclear fuel structural integrity under normal conditions of transport is being conducted in the Republic of Korea. This is the first such research conducted in the Republic of Korea and, thus, previous international studies need to be investigated and will be referred to in the ongoing project. Before 2000, several studies related to measurement of vibration and shock loads on spent nuclear fuel were conducted in the US. US national research institutes conducted uniaxial fuel assembly shaker tests, concrete block tests, and multi-axis fuel assembly tests between 2009 and 2016. In 2017, multi-modal transportation tests including road, sea, and rail transport were also performed by research institutes from the US, Spain and the Republic of Korea. Therefore, test preparation procedures, acceleration and strain measurement results, and finite-element and multi-body dynamics analysis were investigated. Based on the measured strain data, the preliminary conclusion was obtained that the measured strain was too small to cause damage to spent nuclear fuel rods. However, this conclusion is a preliminary conclusion that only reviews part of the results; a detailed review is being conducted in the US. The investigation of international studies on spent nuclear fuel structural integrity tests considering vibration and shock loads under normal conditions of transport in the US will be useful data for the project being conducted in the Republic of Korea.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제21권1호
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• pp.65-68
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• 1999

Multiple facial trauma patient should be carefully treated because of severe bleeding on extraoral and intraoral wound, possibilty of airway obstruction and hypovolemic shock. Hypovolemic shock may be divided to hemorrhagic shock and non-hemorrhagic shock. Also hemorrhagic shock is divided to mild, moderate and severe shock according to the degree of blood volume depletion. Mild shock occurs in blood loss of less than 20% of blood volume and moderate shock does in blood loss of 20-40% of blood volume. And Severe shock occurs in blood loss of more than 40% of blood volume. The goal of emergency care of trauma patient is that respiration and perfusion should be recovered to satisfactory level and that normal vital sign is maintained. We reported the case of multiple facial trauma patient with severe bleeding and hopovolemic shock and metabolic acidosis who was treated with adequate supply of fluid transfusion, intubation, tracheostomy and emergency operation.

• The Bulletin of The Korean Astronomical Society
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• 제39권1호
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• pp.37.2-37.2
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• 2014

Gas in disk galaxies interacts nonlinearly with a underlying stellar spiral potential to form galactic spiral shocks. Numerical simulations typically show that these shocks are unstable to the wiggle instability, forming non-axisymmetric structures with high vorticity. While previous studies suggested that the wiggle instability may arise from the Kelvin-Helmholtz instability or orbit crowding of gas elements near the shock, its physical nature remains uncertain. It was even argued that the wiggle instability is of numerical origin, caused by the inability of a numerical code to resolve a shock that is inclined to numerical grids. In this work, we perform a normal-mode linear stability analysis of galactic spiral shocks as a boundary-value problem. We find that the wiggle instability originates physically from the potential vorticity generation at a distorted shock front. As the gas follows galaxy rotation, it periodically passes through multiple shocks, successively increasing its potential vorticity. This sets up a normal-mode that grows exponentially, with a growth rate comparable to the orbital angular frequency. We show that the results of our linear stability analysis are in good agreement with the those of local hydrodynamic simulations of the wiggle instability.

• Animal cells and systems
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• 제14권4호
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• pp.267-274
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• 2010

The decrease in sperm quality under heat stress causes a great loss in animal husbandry production. In order to reveal the mechanism underlying the sperm quality decrease caused by heat stress, we first established a mild heat-treated mouse model. Then, the sperm quality was identified. Further, the testicular proteome profile was mapped and compared with the control using 2D electrophoresis and mass spectrometry. Finally, the differential expressed proteins involved in the heat stress response were identified by real-time PCR and Western blotting. The results showed that heat stress caused a significant reduction in mouse sperm quality (P<0.05). Further, 52 protein spots on the 2D gel were found to differ between the heat-shocked tissues and the control. Of these spots, some repair proteins which might provide some explanation for the influence on sperm quality were found. We then focused on Bag-1, Hsp40, Hsp60 and Hsp70, which were found to be differently expressed after heat shock (P<0.05). Further analysis in this heat-shocked model suggests numerous potential mechanisms for heat shock-induced spermatogenic disorders.

• Development and Reproduction
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• 제13권3호
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• pp.199-205
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• 2009

HSP70 has widely been induced in in vivo hyperthermia conditions in various organisms to study gene regulation and recently neuroprotectve roles of the induced gene expression under varying conditions. We investigated different responses among various tissues in zebrafish under heat shock to evaluate whether spatial and temporal expression pattern of zebrafish (z) hsp70 in transcriptional and translational level under heat shock stress in different brain regions. Heat shock groups were given for 1 h at $37^{\circ}C$ after recovery by transferring the treated animals back to $28^{\circ}C$ for 1, 2 and 24 h for recovery, respectively. Control (CTRL) group was kept at $28^{\circ}C$. At the end of treatments, five animals were collected and used for isolation of total RNAs and peptides from the corresponding tissues. Expression of zhsp70 mRNA showed different patterns in recovery periods in the tissues including the brain, eye, intestines, muscles, heart and testis by RT-PCR. Unlike the RT-PCR analysis, Northern blot analysis demonstrated nearly 30-fold increase in zhsp70 at 1 h heat shock, suggesting that RT-PCR may not be appropriate in unmasking regulation of the time-dependent zhsp70 expression. In the experiment involving different brain regions, the cerebellum showed gradual activation at 1 h to R1h and decreases in R2h and R24h, while the medulla oblongata and optic tectum showed gradual increase at R1h and decrease at R24h, indicating that different brain tissues respond specifically to heat shock in inducing zhsp70 and recovering from the heat shock status. Western blot analysis also demonstrated that the intracellular levels of zHSP70 in three different brain regions including the cerebellum, medulla oblongata and optic tectum are differently induced and recovered to normal state. These results clearly demonstrate that different regions of the body and the brain tissues are responding differently to heat shock in the aspects of its level of expression and speed of recovery.