• Biomedical Science Letters
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• v.24 no.2
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• pp.108-115
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• 2018

In the present study, results of the identification of Gram-positive bacilli (GPB) were analyzed by using the MALDI-TOF MS technique to score each 2-year blood culture at a university hospital. In addition, 16S rRNA sequence analyses and MALDI-TOF MS results are compared to targeting strains that had been isolated two or more times within the same patient, to evaluate the usefulness of MALDI-TOF MS in GPB identification. According to the cut-off (${\geq}1.7$) criteria, there were 410 (57.5%) reliable strains and 303 (42.5%) non-identified strains among the GPB identification results of 713 strains, using a microflex MALDI Biotyper (Bruker Daltonik GmbH, Bremen, Germany). The isolation appeared most often in the following order: Corynebacterium striatum, Bacillus cereus, Bacillus subtilis, Paenibacillus urinalis, and Listeria monocytogenes. Nearly three-fourths, 66 out of 89 (74.2%) of the strains for Corynebacterium striatum; 44 out of 60 (73.3%) strains for Bacillus cereus; and all (25 out of 25, 100%) Listeria monocytogenes strains were identified by their high scores of 2.0 or higher. Most (293 strains out of 303) non-identified strains were strains isolated only once and not significant as infectious bacilli. A total of 43 out of 50 (86.0%) strains matched and were able to be identified based on the 16 rRNA sequencing comparison results of strains that were isolated twice or more within the same patient and significant as infection bacilli. Non-matching among 5 out of 7 strains was not identified, even with MALDI-TOF MS. In conclusion, GPB can be identified in blood cultures using MALDI-TOF MS. This can be done accurately with ease, rapidly, and at a low cost. It is also thought to be helpful in GPB diagnosis and treatment.

• Nuclear Engineering and Technology
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• v.2 no.2
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• pp.73-84
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• 1970

The metabolism of sterol precurosor in leaves of Salanum andigena grafted between photoinduced and noninduced plant was investigated with the use of (2-$^{14}$ C) mevalonic acid. By the technique of the preparative gas-liquid chromatography, radioactive compounds of squalene, 4,4’-dimethylsterols and 4-demethylsterol were isolated and determined quantitatively. When labeled mevalonic acid n as applied to leaves radioactivity was extensively incorporated into non-saponifiable materials of lipid fraction and aqueous fraction (ethanol-water fraction). Radioactivity of 14C derived from (2-$^{14}$ C) mevalonic acid was transmissible from photoinduced plant to non-induced plant across the graft union, as tuberization hormone was, and incorporated into the sterols of the non-induced plant. Inhibitors of sterol biosynthesis, SK & F 7997 and nicotinic acid, are effective suppressors of tuber growing, if applied to leaves during photoinduction period. The experimental results suggest that certain substance containing isoprene unit, or sterol-like compound may participate in tuber growing.

• Computers and Concrete
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• v.21 no.5
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• pp.495-504
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• 2018

The possibility of earthquakes in vulnerable regions indicates that efficient technique is required for seismic protection of buildings. During the recent decades, the concept is moving towards the insertion of base isolation on seismic prone buildings. So, investigation of structural behavior is a burning topic for buildings to be isolated in base level by bearing device. This study deals with the incorporation of base isolation system and focuses the changes of structural responses for different types of Lead Rubber Bearing (LRB) isolators. A number of sixteen model buildings have been simulated selecting twelve types of bearing systems as well as conventional fixed-base (FB) scheme. The superstructures of the high-rise buildings are represented by finite element assemblage adopting multi-degree of freedoms. Static and dynamic analyses are carried out for FB and base isolated (BI) buildings. The dynamic analysis in finite element package has been performed by the nonlinear time history analysis (THA) based on the site-specific seismic excitation and compared employing eminent earthquakes. The influence of the model type and the alteration in superstructure behavior of the isolated buildings have been duly assessed. The results of the 3D multistory structures show that the lateral forces, displacement, inertia and story accelerations of the superstructure of the seismic prone buildings are significantly reduced due to bearing insertion. The nonlinear dynamic analysis shows 12 to 40% lessening in base shear when LRB is incorporated leading to substantial allowance of horizontal displacement. It is revealed that the LRB isolators might be potential options to diminish the respective floor accelerations, inertia, displacements and base shear whatever the condition coincides. The isolators with lower force intercept but higher isolation period is found to be better for decreasing base shear, floor acceleration and inertia force leading to reduction of structural and non-structural damage. However, LRB with lower isolator period seems to be more effective in dropping displacement at bearing interface aimed at reducing horizontal shift of building structure.

• The Journal of Asian Finance, Economics and Business
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• v.6 no.2
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• pp.173-181
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• 2019

The paper aims to evaluate the combination of strategies that influence the success of New Technology Based Firms (NTBFs) located in isolated areas with resource constraints. We utilize the Kauffman Firm Survey to construct a subset of 224 firms operating in primary metal manufacturing industries and who are located in non-thriving areas. We focus on the impact of technological strategy, in the form of Inward Technology Licensing (ITL), combined with financial strategy, in the form of increased financial slack. Using a negative binomial-specification technique to model these relations, we find that ITL positively impacts internal innovation in the firm and this relation is strengthened by the presence of greater financial slack. This positive impact of financial slack supports the behavioral theory of the firm rather than agency theory in that financial slack enables further innovation rather than stifling it. This research confirms the importance of resource acquisition, suggesting that entrepreneurs may utilize external sources of knowledge in an effort to build a favorable situation when facing the challenges of location. Finally, by presenting evidence showing the compatibility of a financial strategy with a knowledge strategy (ITL), this study emphasizes the importance to entrepreneurs of choosing the proper combination of varying strategies.

• The Journal of the Acoustical Society of Korea
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• v.15 no.4
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• pp.43-49
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• 1996

This research proposes a system for speaker independent Korean continuous speech recognition with 247 DDD area names using keyword spotting technique. The applied recognition algorithm is the Dynamic Programming Neural Network(DPNN) based on the integration of DP and multi-layer perceptron as model that solves time axis distortion and spectral pattern variation in the speech. To improve performance, we classify word model into keyword model and non-keyword model. We make an experiment on postprocessing procedure for the evaluation of system performance. Experiment results are as follows. The recognition rate of the isolated word is 93.45% in speaker dependent case. The recognition rate of the isolated word is 84.05% in speaker independent case. The recognition rate of simple dialogic sentence in keyword spotting experiment is 77.34% as speaker dependent, and 70.63% as speaker independent.

• Nuclear Engineering and Technology
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• v.55 no.12
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• pp.4570-4575
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• 2023

Noise and Radio-frequency interference or RFI causes a significant restriction on the Free induction Decay or FID signal detection of the Nuclear Quadrupole Resonance procedure. Therefore, using this method in non-isolated environments such as industry and ports requires extraordinary measures. For this purpose, noise reduction algorithms and increasing signal-to-noise-and-interference ratio or SNIR have been used. In this research, sodium nitrite has been used as a sample and algorithms have been tested in a non-isolated environment. The resonant frequencies for the 150 g of test sample were measured at 303 K at about 1 MHz and 3.4 MHz. The main novelty in this study was, (1) using two types of antennas in the receiver to improve adaptive noise and interference cancellation, (2) using a separate helical antenna in the transmitter to eliminate the duplexer, (3) estimating the noise before sending the pulse to calculate the weighting factors and reduce the noise by adaptive noise cancellation, (3) reject the interference by blanking algorithm, (4) pulse integration in the frequency domain to increase the SNR, and (5) increasing the detection speed by new pulse integration technique. By interference rejection and noise cancellation, the SNIR is improved to 9.24 dB at 1 MHz and to 7.28 dB at 3.4 MHz, and by pulse integration 44.8 dB FID signal amplification is achieved, and the FID signals are detected at 1.057 MHz and 3.402 MHz at room temperature.

• Proceedings of the Korean Environmental Health Society Conference
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• 2004.06a
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• pp.210-212
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• 2004

This study was conducted to determine the fluoride intakes in 120 preschool children aged $3{\sim}6$ residing in Jumunjin(community water fluoridation area) and Kangnung(non-fluoridation area). The parents duplicated all the diets that their children ingested in a day. The acid-diffusible fluoride in the diet was isolated by the acid-diffusion technique and measured with a fluoride electrode. The mean daily fluoride intakes form all kinds of diet by children residing in Jumunjin and Kangnung were $0.445{\pm}0.354mg/day$ and $0.131{\pm}0.097mg/day$, respectively. It is concluded from this investigtion that the amount of fluoride intake of children living in Jumunjin(fluoridated areas) did not exceed the upper intake level designated by the Institute of medicine of the US National Academy of Science to avoid the risk of dental fluorosis(2.2mg/day in 4- to 8-year-olds).

• Maxillofacial Plastic and Reconstructive Surgery
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• v.31 no.3
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• pp.198-206
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• 2009

Purpose: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal's mandibular defects. Materials and Methods: We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4 weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated. $1{\times}10^7\;cells/100\;{\mu}l$ of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold. In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4 weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the SKP-grafted group compared to the control group. Conclusion: These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold can serve as a useful alternative to bone grafting technique.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.521-524
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• 2001

A method using PCR was developed for the monitoring of glyphosate tolerant soybean (GTS) produced by the DNA recombination technique. We designed 3 pairs of specific oligonucleotide primers based on the gene sequences inserted in soybean and in lectin and ferritin genes as internal standards. Template DNAs were isolated from soybeans by the modified hexadecyl trimethyl ammonium bromide (CTAB)method and used for PCR with different primer sets. PCR, used with specific primer sets for GTS detection, showed the amplified DNA fragments with GTS template DNA but no product showed with non-GTS template. PCR amplified products were confirmed by DNA sequencing and were detected for up to 0.05% of GTS template DNA.

• Proceedings of the KSR Conference
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• 2011.05a
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• pp.1161-1166
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• 2011

A camera is mounted vertically downwards viewing the people heads from the top. This configuration is successful in people counting technique especially when only a few isolated people pass through a counting region in a non-crowded situation. Thus, this paper describes object counting which detects and count moving people using mixture of gaussian and motion vector. This method is intended to estimates the number of people in outdoor environment. This method use single gaussian background modeling which is more robust an noise and has adaptiveness. The experimental results that is based on mixture of gaussian and motion vector is also helpful to design intelligent surveillance.