• Title/Summary/Keyword: Non-injection

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Prevention of Oak Wilt by Tree Injection of Culture Suspension of an Antifungal Microorganism, Streptomyces blastmyceticus against Oak Wilt Fungus, Raffaelea quercus-mongolicae

  • Lee, Jin Heung;Hong, A Reum;Yun, Ji Ho;Seo, Sang Tae;Lee, Jong Kyu
    • Journal of Forest and Environmental Science
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    • v.34 no.5
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    • pp.376-381
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    • 2018
  • For the control of oak wilt caused by Raffaelea quercus-mongolicae, an antifungal microorganism, Streptomyces blastmyceticus, was used as a potential agent. Culture suspension of S. blastmyceticus was injected into Quercus mongolicae in the research forest of Kangwon National University by $ChemJet^{(R)}$ trunk injection and Macro-infusion at root flare injection. $Alamo^{(R)}$ (a.i., propiconazole 14.5%), a fungicide currently used for the control of oak wilt in USA, was also treated by both methods to compare the efficacy. For preventive efficacy, culture suspension of the pathogen was inoculated at 1 month after injection of either agent. Tested trees were cut at 3 months after treatment, stained with 1% Fuchsin acid, and then non-conductive area (NCA) and re-isolation frequency (RIF) of oak wilt fungus were compared among treatments. While NCA was the highest as 47.3% in pathogen only treatment, it was the lowest as 16.0% in sterilized water treatment by Macro-infusion. NCAs of Alamo treatment by Macro-infusion and ChemJet injection were 25.3% and 32.1%, respectively. NCA of S.blastmyceticus treatment by ChemJet injection was 32.3%, similar with Alamo treatment's by ChemJet injection. All treatments by either injection method showed significantly lower NCA compared to the pathogen only treatment. These results indicate that S. blastmyceticus injection shows the preventive efficacy against oak wilt disease by suppressing the growth of pathogen injected. NCA of Macro-infusion injection of sterilized water was lower as 16.0%, compared to 21.3% of ChemJet injection. It means that Macro-infusion is more effective in translocation of sterilized water than ChemJet injection by even distribution. RIF from wood discs of treated trees showed high in pathogen only treatment, but relatively low in S. blastmyceticus treatment. RIF results were correlated with NCA results. From the above results, it was confirmed that S. blastmyceticus showed preventive efficacy against oak wilt disease by ChemJet trunk or Macro-infusion at root flare injection.

Effects of Reduced Glutathione and Ginseng Extract on Non-Protein Sulfhydryl, and Non-Protein Disulfide of Mouse Liver and Blood Following Whole Body X-Irradiation (Reduced glutathione 및 인삼추출액(人蔘抽出液)이 X-선전신조사(線全身照射)를 입은 마우스 간조직(肝組織) 및 혈중(血中) NP-SH 및 NP-SS에 미치는 영향(影響))

  • Oh, Jang-Suk
    • The Korean Journal of Physiology
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    • v.6 no.2
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    • pp.57-63
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    • 1972
  • In an attempt to better understand the radioprotective effect of reduced glutathione(GSH), and to observe a possible radioprotective effect of Ginseng extract, whole body X-irradiation of 1,200 r was administered to the mouse either independently or immediately following the injection of GSH or Ginseng extract to the mouse intraperitoneally. The non-protein sulfhydryl (NP-SH) and non-protein disulfide (NP-SS) levels of the liver, and NP-SH level of NP-SH of the blood of the mouse were measured at 30, 60 and 120 minutes, and results were compared with the normal. The results thus obtained are summarized as follows; 1) The normal values of NP-SH and NP-SS of the mouse liver were $5.90{\pm}0.46\;{\mu}\;mol/gm\;wet\;wt.,\;and\;3.02{\pm}0.42\;{\mu}\;mol/ml$ wet wt., respectively, and the normal value of NP-SH of NP-SH of the mouse blood was $3.98{\pm}1.29\;{\mu}\;mol/ml$ 2) The injection of both GSH and Ginseng extract produced the highest values of NP-SH in the liver at 30 minutes, but a gradual decrease to the normal was observed thereafter. When X-irradiation alone was applied, the liver NP-SH value was lower than the normal at 60 minutes post-irradiation and thereafter. When Ginseng extract was injected immediately prior to X-irradiation, the liver NP-SH was lower than the normal throughout the experiment with the lowest value at 60 minutes. However, the combination of GSH and X-irradiation produced higher than the normal values throughout the entire experiment. 3) The liver NP-SS value was most significantly elevated at 30 minutes after the injection of GSH, hut the recovery to the normal was observed thereafter. The injection of Ginseng extract produced slightly higher liver NP-SS values at 30 and 60 minutes, but the value at 120 minutes was similar to the normal. The single application of X-irradiation resulted in the lower then normal liver NP-SS values throughout the entire experiment. When GSH was injected price to X-irradiation, the liver NP-SS values were higher than the normal at 30 and 60 minutes followed b the recovery to the normal at 120 minutes. The combination of Ginseng extract and X-irradiation showed generally lower liver NP-SS values throughout the experiment. 4) The blood NP-SH showed the higher than the normal values in all the experimental groups except when GSH was injected prior to X-irradiation alone produced e significantly elevated blood NP-SS value at 30 minutes post-irradiation.

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A Experimental Study of the Kinetic Characteristics of Methane Hydrate (메탄 하이드레이트 동적특성에 대한 실험적 연구)

  • Kim, Nam-Jin;Chun, Won-Gee
    • Journal of the Korean Solar Energy Society
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    • v.26 no.2
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    • pp.19-25
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    • 2006
  • Methane hydrate, non-polluting new energy resource, satisfies requirement and considered as a precious resource that can prevent the global warming. Fortunately, there are abundant resources of methane hydrate distribute in the earth widely. Therefore, developing the techniques that can utilize these gases effectively is highly desired. The work in this paper here is to develop a skill which can transport and store methane hydrate. As a first step, the equilibrium experiment was carried out by increasing temperatures in the cell at fixed pressures. The influence of gas consumption rates under variable degree of subcooling, stirring and water injection has been investigated formation to clarify kinetic characteristics of the hydrate. The results of present investigation showed that the enhancements of the hydrate formation in terms of the gas/water ratio are closely related to operational pressure, temperature, degrees of subcooling, and water injection.

Experimental Study on Supersonic Combustion Phenomena in the Cavity Duct by the Supersonic Inflow Conditions (초음속 유입 유동 조건에 따른 공동을 포함한 덕트 내 초음속 연소 현상에 관한 실험적 연구)

  • Jeong, Eun-Ju;Jeung, In-Seuck;O'Byrne, Sean;Houwing, A.F.P.
    • 한국연소학회:학술대회논문집
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    • 2006.10a
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    • pp.209-219
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    • 2006
  • The supersonic combustion experiments are carried out using T3 free-piston shock tunnel. Different shock tube fill pressures have various inflow conditions. $15^{\circ}$ inclined hydrogen fuel injection is located before the cavity. Oblique shock is generated at the trailing edge of the cavity and reflects off the top and bottom wall. For non-reacting flow, static pressures in low equivalence ratio are similar to those in no fuel injection. As equivalence ratio is increased, static pressures are increased in the duct. In the similar equivalence ratio, static pressures are increased when total enthalpy is decreased. For reacting flow, the flame is occurred near the cavity. The combustion is weak locally in the middle of the duct. The up and down pressure distribution in the duct means that the supersonic combustion is generated.

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Deformation Analysis of Injection Molded Articles due to In-mold Residual Stress and Cooling after Ejection (사출 성형품의 금형내 잔류응력과 이형후 냉각에 의한 후변형 해석)

  • Yang, Sang-Sik;Kwon, Tai-Hun
    • Proceedings of the KSME Conference
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    • 2001.06c
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    • pp.251-256
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    • 2001
  • Deformation analysis of injection molded articles whose geometry is considered as the assembly of the thin flat plates has been conducted. For the in-mold analysis, thermo-viscoelastic stress calculation of rheologically simple amorphous polymer and in-mold deformation calculation considering the in-plane mold constraint has been done. Free volume theory has been used for the non-equilibrium density state by the fast cooling. At ejection, the redistribution of stress together with instantaneous deformation has been considered. During out-of-mold cooling after ejection, thermoelastic model based on the effective temperature has been adopted for the calculation of deformation. Two typical mold geometries are used to test the numerical simulation.

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Local Irritation of DA-3002, an Authentic Recombinant Human Growth Hormone, in Rabbits (토끼에 대한 천연형인성장호르몬 DA-3002의 국소자극성)

  • 김옥진;안병옥;인순복;김원배;양중익
    • Biomolecules & Therapeutics
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    • v.3 no.1
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    • pp.63-71
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    • 1995
  • The local irritation studies of DA-3002, an authentic recombinant human growth hormone (rhGH), were carried out in rabbits after the following treatment ; application into the conjunctival sac of the eye (single), single subcutaneous and intramuscular injection, 7-day repeated subcutaneous and intramuscular injection. The results obtained were as follows. In the result of ocular irritation test, 0.16% solution of DA-3002 could be considered as a non-irritating material. In single subcutaneous and intramuscular irritation test, the irritancy of 0.16% DA-3002 solution was not so much different from that of saline. The local irritation of DA-3002 by 7-day repeated injection was negligible and similar to that of saline by both subcutaneous and intramuscular routes. These results suggest that DA-3002 has no irritating activity when injected through subcutaneous or intramuscular route for clinical practice as 0.16% solution.

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Effects of Core Pin Shape on the Filling Imbalances of PA6 Molding (러너 코어핀 형상이 PA6 성형품의 충전불균형도에 미치는 영향)

  • Jeong Y.D.;Kang C.M.;Je D.K.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.706-709
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    • 2005
  • Despite geometrical balanced runner system, filling imbalances between cavity to cavity have always been observed in multi-cavity injection mold. These filling imbalances are results from non-symmetrical shear rate distribution within melt as it flows through the runner system. It has been possible to decrease filling imbalance by optimizing processing conditions, but it has not completely eliminated this phenomenon during the injection molding processing. This paper presents a solution of these filling imbalances by using runner core pin which creates a symmetrical shear distribution within runner and the effects on filling imbalance when modifying a shape of runner core pin. As a result of using runner core pin, a remarkable improvement in reducing filling imbalance was confirmed. In addition we investigated how filling imbalances were affected by shape of runner core pin.

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Intracranial Chronic Subdural Hematoma Presenting with Intractable Headache after Cervical Epidural Steroid Injection

  • Kim, Myungsoo;Park, Ki-Su
    • Journal of Korean Neurosurgical Society
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    • v.58 no.2
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    • pp.144-146
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    • 2015
  • Postdural punctural headache (PDPH) following spinal anesthesia is due to intracranial hypotension caused by cerebrospinal fluid (CSF) leakage, and it is occasionally accompanied by an intracranial hematoma. To the best of our knowledge, an intracranial chronic subdural hematoma (CSDH) presenting with an intractable headache after a cervical epidural steroid injection (ESI) has not been reported. A 39-year-old woman without any history of trauma underwent a cervical ESI for a herniated nucleus pulposus at the C5-6 level. One month later, she presented with a severe headache that was not relieved by analgesic medication, which changed in character from being positional to non-positional during the preceding month. Brain magnetic resonance imaging revealed a CSDH along the left convexity. Emergency burr-hole drainage was performed and the headache abated. This report indicates that an intracranial CSDH should be considered a possible complication after ESI. In addition, the event of an intractable and changing PDPH after ESI suggests further evaluation for diagnosis of an intracranial hematoma.

LCD 제조용 스퍼터링 장비의 비접촉식 유리평판 이송장치에 대한 수치적 연구

  • Gang, U-Jin;Im, Ik-Tae;Kim, U-Seung
    • Proceedings of the Korean Society Of Semiconductor Equipment Technology
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    • 2007.06a
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    • pp.149-156
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    • 2007
  • Non-contact transportation of the large-sized glass plate using air-cushion is considered for sputtering system of LCD panel. The Argon gas from second gas injection holes is injected to levitate and transport the glass plate. Low maximum pressure and uniform pressure distribution on the bottom surface of the glass plate must be maintained for stable levitation and transportation of the glass plate. Therefore, the analysis of fluid flow between the glass plate and the air-pad is numerically performed for varying space between the injection holes in this study. The pressure uniformity on the bottom surface of the glass plate is evaluated for overall glass plate. The distance between the injection holes must be designed below 90 mm for obtaining the low maximum pressure and uniform pressure distribution.

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An Analysis on the KSTAR neutral beam injection line (KSTAR 중성입자빔 수송라인 해석)

  • 임기학;김진춘;권경훈;조승연
    • Journal of the Korean Vacuum Society
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    • v.8 no.4B
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    • pp.556-564
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    • 1999
  • The analysis on heat fluxed on and transmission efficiencies by the collimators of neutral beam injection lines in KSTAR tokamak device has been carried out. And a mathematical model describing non-Gaussian beam distribution profile has been established. A neutral beam injection device is composed of 3 separate ion sources and corresponding beam transport lines, which deal with 7.8 MW of beam power, respectively. The divergence angles of ion beam are $1.2^{\circ}$and $0.5^{\circ}$, in vertical and horizontal directions, respectively. The maximum normal heat load on source exit scraper is 9.1 kW/$\textrm{cm}^2$ and net beam transmission efficiency is ~28%. The effect of misalignment of ion source and scrapers on the scraper heat load and beam transmission also has been analyzed.

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