• Analytical Science and Technology
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• v.16 no.4
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• pp.325-332
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• 2003

To analyze co-planar PCBs in food, the isotopic dilution method by high resolution gas chromatography/high resolution mass spectrometry was applied. Among 209 PCB congeners, 12 congeners (#77, #81, #126, #169, #105, #114, #118, #123, #156, #157, #167 and #189) were chosen as target compounds that were toxic congeners re-assessed by WHO in 1998. Milk and milk products including cheese and butter were collected as food samples. Samples were homogenized, spiked with the known amount of the standard mixture and extracted. After extraction, extracts were cleaned up by sulfuric acid impregnated silica gel, purified on silica gel and alumina column chromatography and then analyzed by HRGC/HRMS. As results, the overall recoveries were ranged from 83% to 106% and the limit of detection was about 0.1 pg/g at signal/noise>3. Levels of targets in the selected food samples were 0.001~0.107 pgWHO-TEQ/g.

• Journal of the Korean Society of Food Culture
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• v.35 no.4
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• pp.400-405
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• 2020

Evodiae Fructus is the dried unripe fruit of Evodia rutaecarpa, and has traditionally been used for treating stomachache and diarrhea. Evodiamine and rutaecarpine, the major biologically active compounds of Evodiae Fructus, are reported to have anti-oxidative and anti-inflammatory effects, as well as inhibit proliferation and metastasis of various cancer cells. The current study investigates the anti-oxidative and anti-cancer effects of the Evodiae Fructus extract, considering varying concentrations of methanol extraction (40, 80, and 95%). High contents of total phenolic compounds were determined in the order of extracts 80, 95, and 40%. Evaluating contents of the 95, 80, and 40% extracts revealed 36.77, 7.29, and 1.86 ㎍/mg evodiamine, respectively, and 53.02, 17.16, and 3.79 ㎍/mg rutaecarpine, respectively, with the highest content of both compounds obtained in the 95% extract. DPPH radical scavenging activity was observed to be inversely proportional to the contents of total phenolic compounds, with decreasing SC₅₀ values obtained in the order 80, 95, and 40% extract. The 95 and 80% extracts exerted toxicity to AGS gastric cancer cells, but the 40% extract was non-toxic. Evodiamine is a known anti-cancer agent, and could be responsible for the observed toxicity. Cleavage of PARP, and Caspase-3, -7, -8 and -9 was observed in the 95% extract-treated AGS cells, indicating that cell toxicity exerted by the 95% extract could be attributed to apoptosis.

• Parasites, Hosts and Diseases
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• v.55 no.4
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• pp.409-416
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• 2017

The high prevalence of pediculosis capitis, commonly known as head lice (Pediculus humanus capitis) infestation, has led to the preparation of a community-based pediculicidal ointment, which is made of common household items and the extract of Tinospora crispa stem. The present study aimed to evaluate the safety, efficacy, and physicochemical characteristics of the T. crispa pediculicidal ointment. The physicochemical properties of the ointment were characterized, and safety was determined using acute dermal irritation test (OECD 404), while the efficacy was assessed using an in vitro pediculicidal assay. Furthermore, the chemical compounds present in T. crispa were identified using liquid-liquid extraction followed by ultra-performance liquid chromatography quadruple time-of-flight mass spectrometric (UPLC-qTOF/MS) analysis. The community-based ointment formulation was light yellow in color, homogeneous, smooth, with distinct aromatic odor and pH of $6.92{\pm}0.09$. It has spreadability value of $15.04{\pm}0.98g{\cdot}cm/sec$ and has thixotropic behavior. It was also found to be non-irritant, with a primary irritation index value of 0.15. Moreover, it was comparable to the pediculicidal activity of the positive control $Kwell^{(R)}$, a commercially available 1% permethrin shampoo (P>0.05), and was significantly different to the activity of the negative control ointment, a mixture of palm oil and candle wax (P<0.05). These findings suggested that the community-based T. crispa pediculicidal ointment is safe and effective, having acceptable physicochemical characteristics. Its activity can be attributed to the presence of compounds moupinamide and physalin I.

• Journal of Digital Contents Society
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• v.18 no.6
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• pp.1033-1040
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• 2017

Artists use artistic features of paintings to provide various emotions in paintings. These features may be simply color and texture, but they can move on to form a composition or a symmetry. Through these features, people can feel various emotions when enjoying paintings. Even though they are using these features, there are paintings that are not readily accessible to non-extractable experts. This is because the analysis of features is not intuitive. In this paper, we want to produce content that matches paintings and music. This helps user to understand painting easily with paintings and matched music.

• Microbiology and Biotechnology Letters
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• v.43 no.3
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• pp.177-186
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• 2015

With a view to supporting the provisions of the current Korean food code for the detection of Benzo[a]pyrene, various analytical methods of detection in foods were evaluated and established in terms of linearity, limits of detection/quantitation, efficiency, and accuracy, amongst others. It was observed that to improve the technologies involved in the application of these methods, complicated and combined preparation processes of foods, including extraction, separation and purification, have been the main focus of efforts at optimization. Recently, on-site quick reaction for the detection of hazardous substances in the environment and food materials aims at developing simplified examination processes, such as lable-free and non-invasive technological analysis, to reduce the costs and time involved in the examination. Herein, current benzo[a]pyrene detection methods are reviewed in addition to new Raman spectroscopy-based trials established to pursue improve the speed, simplicity and suitability of testing.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.61.2-61.2
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• 2015

In the past decades, green energy, such as solar energy, wind power, hydropower, biomass energy, geothermal energy, and so on, has been widely investigated and developed to solve energy shortage. Recently, organic solar cells have attracted much attention, because they have many advantages, including low-cost, flexibility, light weight, and easy fabrication [1-3]. Organic solar cells are as a potential candidate of the next generation solar cells. In this abstract, to improve the power conversion efficiency and the stability, the inverted polymer solar cells with various structures were developed [4-6]. The novel cell structures included the P3HT:PCBM inverted polymer solar cells with AZO nanorods array, with pentacene-doped active layer, and with extra P3HT interfacial layer and PCBM interfacial layer. These three difference structures could respectively improve the performance of the P3HT:PCBM inverted polymer solar cells. For the inverted polymer solar cells with AZO nanorods array as the electronic transportation layer, by using the nanorod structure, the improvement of carrier collection and carrier extraction capabilities could be expected due to an increase in contact area between the nanorod array and the active layer. For the inverted polymer solar cells with pentacene-doped active layer, the hole-electron mobility in the active layer could be balanced by doping pentacene contents. The active layer with the balanced hole-electron mobility could reduce the carrier recombination in the active layers to enhance the photocurrent of the resulting inverted polymer solar cells. For the inverted polymer solar cells with extra P3HT and PCBM interfacial layers, the extra PCBM and P3HT interfacial layers could respectively improve the electron transport and hole transport. The extra PCBM interfacial layer served another function was that led more P3HT moving to the top side of the absorption layer, which reduced the non-continuous pathways of P3HT. It indicated that the recombination centers could be further reduced in the absorption layer. The extra P3HT interfacial layer could let the hole be more easily transported to the MoO3 hole transport layer. The high performance of the novel P3HT:PCBM inverted polymer solar cells with various structures were obtained.

• Journal of Korea Spatial Information System Society
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• v.7 no.1 s.13
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• pp.13-24
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• 2005

Objects, which change their positions over time such as cars, are called moving objects. Trajectories of a moving object have large volumes because trajectories are accumulated. Efficient indexing techniques for searching these large volumes of trajectories are needed in the moving object databases. Especially the TB-tree which supports bundling trajectories is suitable for processing combined queries which have 2 steps: first step is selecting trajectories (range search), next is selecting the parts of each trajectory (trajectory search). But the TB-tree has unnecessary disk accesses cause of lack of spatial discrimination in range queries. In this paper, we propose and implement the splitting polity which can reduce dead spaces of non-leaf node in order to process range queries efficiently. The policy has better performance about range queries than the TB-tree as well as the advantages of the TB-tree, such as highly space utilization and efficient trajectory extraction. This paper shows that the newly proposed split policy has better performance in processing the range queries than that of the TB-tree by experimental evaluation.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.44 no.2 s.314
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• pp.82-92
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• 2007

This paper describes a novel global shape (GS) feature based on radial basis function network (RBFN) and the extraction method of the proposed feature for 3D face recognition. RBFN is the weighted sum of RBfs, it well present the non-linearity of a facial shape using the linear combination of RBFs. It is the proposed facial feature that the weights of RBFN learned by the horizontal profiles of a face. RBFN based feature expresses the locality of the facial shape even if it is GS feature, and it reduces the feature complexity like existing global methods. And it also get the smoothing effect of the facial shape. Through the experiments, we get 94.7% using the proposed feature and hidden markov model (HMM) to match the features for 100 gallery set with those for 300 test set.

• Journal of the Korean Institute of Intelligent Systems
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• v.18 no.5
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• pp.685-691
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• 2008

The selection of classification boundaries in fuzzy rule- based classification systems is an important and difficult problem. So various methods based on learning processes such as neural network, genetic algorithm, and so on have been proposed for it. In a previous study, we pointed out the limitation of the methods and discussed a method for fuzzy partitioning in the overlapped region on feature space in order to overcome the time-consuming when the additional parameters for tuning fuzzy membership functions are necessary. In this paper, we propose a method to determine three types of classification boundaries(i.e., non-overlapping, overlapping, and a boundary point) on the basis of statistical information of the given dataset without learning by extending the method described in the study. Finally, we show the effectiveness of the proposed method through experimental results applied to pattern classification problems using the modified IRIS and standard IRIS datasets.

• Journal of Oral Medicine and Pain
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• v.25 no.1
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• pp.29-39
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• 2000

Lysyl Oxidase from fetal bovine aorta was purified to homogenity using extraction, Sephacryl S200HR chromatography, Hydropore AX ion-exchange high performance liquid column chromatography, Cibacron blue affinity chromatography, and Sephacryl S-300 HR chromatography in the presence of protease inhibitor. The purified enzyme was active toward lathyritic collagen as well as elastin and was sensitive to aminonitriles such as BAPN. Upon Sephacryl S-300 HR chromatography, the enzyme was eluted as a peak with a $K_{av}$ value of 0.45 (65% of $V_t$ ) and it eluted from high performance liquid ion-exchange column (Hydropore |AX) at single position (ionic strength, I = 0.1~0.15). Once purified, it showed one band upon SDS-PAGE. It migrated to a band the mobility of which corresponded to a Mr of 33,500 upon reduction while it migrated to a 24,500 Mr position under the non-reducing condition. In constrast to other reports, it is concluded that fetal bovine aorta contains only one type of lysy oxidase.