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A Study on the Growth Inhibition of Human Colon Cancer Cells by Eucommial Lea Extract (두충의 인체 암세포 증식억제 효과 연구)

  • 황우익
    • The Korean Journal of Food And Nutrition
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    • v.5 no.1
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    • pp.13-22
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    • 1992
  • This study was divised to observe the inhibitory effect of growth rate of human colon cancer cells by Eucommial leaf extract, in vitro. Three species of human colon cancer cells, HRT-18, HCT-48 and HT-29, were used for the experiment. Each extract of Eucommial leaf was prepared by extraction with water, 95% alcohol, acetone, chloroform and petroleum ether, and then the inhibitory effect of each extract on the growth rate of cells was compared with control group and each other. The experimental results obtained are summarized as follows; 1. Inhibitory effects on growth rate of human colon cancer fells were strongest in the petroleum ether extract and next in the chloroform extract. 2. Inhibitory effects on the growth rate of the cancer cells by extracts of water, 95% alcohol and acetone were weaker than that of petroleum ether and chloroform. 3. Inhibitory effect of each extract on the cancer cell growth was shown most strong activity in HT-29, and was in order of HRT 18 and HCT-48. In view of the results, it could be suggested that inhibitory effects of non-polar solvent's extracts against the cancer cell growth were more stronger than that of polar solvents and the effects were indicated difference according to the species of the cells.

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A Study of Smoke Exhaust Facility Operation of Subway Platform with Installation of Platform Screen Door (승강장 스크린도어 설치에 따른 배연설비운영에 대한 연구)

  • Rie, Dong-Ho;Ko, Jae-Woong;Kim, Ha-Young
    • Journal of the Korean Society of Safety
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    • v.21 no.1 s.73
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    • pp.35-40
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    • 2006
  • A study on fire phenomena in a subway transit mass station has been carried out as a part of disaster prevention plan at the subway station. The ventilation facilities installed in both the platform and the trackway are designed to convert into a smoke exhaust system in emergency situation, creating such an environment as necessary for evacuation. 3 dimensional Numerical Simulations based on the CFD are carried out using a simulation tool, Fire Dynamic Simulator. Additionally, four different vent modes are made and performances are compared with the original design mode and each other to find better operation of vents at both the platform and the trackway in case of fire. From the result, an vent operational characteristics under the condition of installed PSD is clarified for the effective smoke and heat removal from the platform area compared with non installed PSD.

Immunofluorescence Microscopy and Biochemical Characterization of Two Nuclear Envelope Proteins of Amoeba proteus by Using a Monoclonal Antibody (단항체를 이용한 아메바(Amoeba proteus) 의 2종 핵막 단백질에 대한 면역형광현미경적 및 생화학적 특성 조사)

  • 안태인;유시욱조양래
    • The Korean Journal of Zoology
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    • v.34 no.1
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    • pp.44-53
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    • 1991
  • Distribution of the antigens during the cell cycle of amoebae was followed by immunof-luorescence microscopy using a monoclonal antibody against the nucleus as a probe. While the cells were in the interphase, the antigen was localized on the nucleus membrane. But it was dispersed all over the cytoplasm during mitosis and cytokinesis. The molecular weights of the immunoreacted antigens were 210 KD and 190 KD as determined by SDS PAGE and western blotting of the purified nuclei. The antigens were not soluble in non-ionic detergent, but were released from the nucleus by incubation with 0.05 M sodium carbonate, pH 10.6 or with 8 M urea at serial chemical extraction. Thus the antigens appeared to be peripheral proteins of the nurBeus envelope. The isoelectic point of both antigens was 7.64 as determined by 2 D PAGE and transfer blotting. Considering the peiipherd association with the nucleus membrane and the dispersed distribution during mitosis, the antigens could be lamin like proteins. Hourever, it appears also possible that they are the component molecules of the unusually structured aurous lamina of amoeba nucleus since they have the large molecular weight and the basic pl.

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Customized Information Analysis System Using National Defense News Data (국방 기사 데이터를 이용한 맞춤형 정보 분석 시스템)

  • Choi, Jung-Whoan;Lim, Chea-O
    • The Journal of the Korea Contents Association
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    • v.10 no.12
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    • pp.457-465
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    • 2010
  • Customized information analysis system is a software system that can help to extract useful information from non-structured natural language data, process the information to customized form, and provide future forecast and reasoning information. To implement the information analysis system, we need natural language processing technology to analyze natural language, information extraction technology to detect necessary entity and its relationship from text, and data mining technology to discover new and unknown information from extracting data. This paper suggest virtual customized information analysis system processing national defense news data and introduce base technologies for information analysis.

Adaptive Segment-length Thresholding for Map Contour Extraction (등고선 추출을 위한 적응적 길이 임계화)

  • 박천주;오명관;전병민
    • The Journal of the Korea Contents Association
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    • v.3 no.4
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    • pp.23-28
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    • 2003
  • This paper describes, in order to extract contour from topographic map image, an adaptive segment-length thresholding using a threshold depended on target image. First of all, after recognizing the primary symbols and detecting two edges from the projection histogram of the elevation value area, the threshold value is determined by the distance between the edges. Then, the subdivision is peformed by searching a branch point and erasing its neighboring Hack pixels. And contour components are extracted by segment-length thresholding. The experimental result shows that the final image contains non-contour component of 2.41% and contour one of 97.59%.

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Characterization of Density-of-States in Polymer-based Organic Thin Film Transistors and Implementation into TCAD Simulator

  • Kim, Jaehyeong;Jang, Jaeman;Bae, Minkyung;Lee, Jaewook;Kim, Woojoon;Hur, Inseok;Jeong, Hyun Kwang;Kim, Dong Myong;Kim, Dae Hwan
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.13 no.1
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    • pp.43-47
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    • 2013
  • In this work, we report extraction of the density-of-states (DOS) in polymer-based organic thin film transistors through the multi-frequency C-V spectroscopy. Extracted DOS is implemented into a TCAD simulator and obtained a consistent output curves with non-linear characteristics considering the contact resistance effect. We employed a Schottky contact model for the source and drain to fully reproduce a strong nonlinearity with proper physical mechanisms in the output characteristics even under a very small drain biases. For experimental verification of the model and extracted DOS, 2 different OTFTs (P3HT and PQT-12) are employed. By controlling the Schottky contact model parameters in the TCAD simulator, we accurately reproduced the nonlinearity in the output characteristics of OTFT.

Versatilities of Calix[4]pyrrole Based Anion Receptors

  • Lee, Chang-Hee
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.768-778
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    • 2011
  • Calixpyrroles and related macrocycles are non-planer synthetic anion receptors that have attracted considerable attentions in recent years. Although the synthesis of calix[4]pyrrole (known as meso-octamethylporphyrinogen) was reported more than 100 years ago, the anion binding properties were first discovered in 1996. The simple calix[4]pyrroles can be synthesized in single step in high yield by condensation of pyrrole with acetone. The compounds showed preferential binding for halide anions including fluoride, phosphate, carboxylate, and chloride in organic media. Efforts to improve the anion affinity of calix[4]pyrrole and to enhance its selectivity have led to the synthesis of a variety of new calixpyrrole derivatives. Among the various modifications, introduction of straps on one side of the calix[4]pyrroles are the most effective. Incorporation of aromatic rings other than pyrroles also exhibited interesting binding behaviour. Introduction of signalling units as part of the strapping element enable to detect the anions on chromogenic or fluorogenic fashion. Finding of the anion transport properties across the membrane and cytotoxic effects of the calix[4]pyrroles open new window for calixpyrrole-related research. The polymer-incorporated systems have also been employed as anion complexants in solvent-solvent extraction. These old, yet easy-to-make macrocycles have well advanced more recently with the discovery of the ion-pair complexation properties. In this review, the synthetic developments and anion binding properties of calixpyrroles for the last decades will be discussed and will cover the advances in calixpyrrole chemistry.

Review on the induced seismic event for artificial reservoir (인공저류층 생성을 위한 유도진동에 관한 사전연구)

  • Jeon, Jong-Ug;Myoung, Woo-Ho;Kim, Young-Deug
    • Journal of the Korean Society for Geothermal and Hydrothermal Energy
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    • v.8 no.2
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    • pp.55-60
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    • 2012
  • In many cases, geothemal wells will not be opened up a geothermal reservoir under such conditions that an extraction of geothermal energy is economically viable without any further measures. Geothermal wells often have to be stimulated, in order to increase productivity. For the non-volcanic area, such as Korea, the hydraulic stimulation is necessary to complete geothermal power plant. The analysis of induced seismic event showed that the thermal resource might have a much wider extent and a much higher generation potential than previously assumed. In order to record compressional and shear waves emitted during fracture stimulation, three-component geophones are placed in a seismometer. The recorded data from one seismometer is the convolution of the source magnitude, the transmission media, and the sensitivity of the instrument.

Signal analysis of surface discharge and electromagnetic wave for insulator by kaolin contamination (카올린으로 오손된 애자의 표면방전 및 방사전자파의 신호 분석)

  • Park, Jae-Jun
    • The Journal of Information Technology
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    • v.7 no.3
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    • pp.113-118
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    • 2004
  • Recently, diagnosis techniques have been investigated to detect a partial discharge associated with a dielectric material defect in a high voltage electrical apparatus. However, the properties of detection technique of PD aren't completely understood because the physical process of PD. Therefore, this paper analyzes the process on Surface Discharge of Polymer Insulator using Wavelet transform. Wavelet transform provides a direct quantitative measure of spectral content in the time frequency domain. As it is important to develop a non-contact method for detecting the Contamination Degree, this research analyzes the electromagnetic waves emitted from PD using Wavelet transform. This result experimentally shows the process of PD as a two-dimensional distribution in the time-frequency domain. The method is shown to be useful for detecting prediction of contamination degree.

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MiRPI: Portable Software to Identify Conserved miRNAs, Targets and to Calculate Precursor Statistics

  • Vignesh, Dhandapani;Parameswari, Paul;Im, Su-Bin;Kim, Hae-Jin;Lim, Yong-Pyo
    • Genomics & Informatics
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    • v.9 no.1
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    • pp.39-43
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    • 2011
  • MicroRNAs (miRNAs) are recently discovered small RNA molecules usually resulting in translational repression and gene silencing. Despite the fact that specific cloning of small RNA's is a method in practice, computational identification of miRNA's has been a major focus recent days, since is a rapid process following AB initio and sequence alignment methods. Here we developed new software called MiRPI that aims to identify the highly conserved miRNAs without any mismatches from given fasta formatted gene sequences by using non-repeated miRNA dataset of the user's interest. The new window embedded with the software is used to identify the targets for inputted mature miRNAs in the mRNA sequences. Also MiRPI is designed to measure the precursor miRNA statistics, majorly focusing the Adjusted Minimum Folding free Energy (AMFE) and Minimum Folding free Energy Index (MFEI), the most important parameters in miRNA confirmation. MiRPI is developed by PERL (Practical Extraction and Report Language) and Tk (Tool kit widgets) scripting languages. It is user friendly, portable offline software that works in all windows OS, sized to 3 MB.