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The treatment of Non-obstructive Azoospermia (비폐쇄성 무정자증의 치료)

  • Seo, Ju-Tae;Park, Yong-Seog;Kim, Jong-Hyun;Lee, You-Sik;Jun, Jin-Hyun;Lee, Ho-Joon;Son, Il-Pyo;Kang, Inn-Soo;Jun, Jong-Young
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.1
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    • pp.95-99
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    • 1997
  • Irreparable obstructive azoospermic patients can be treated successfully with microsurgical epididymal sperm aspiration(MESA) or testicular sperm extraction (TESE) by intracytoplasmic sperm injection(ICSI). Obstructive azoospermic patients generally have normal spermatogenesis. The aim of this study was to see if any spermatozoa could be retrieved from non-obstructive azoospermia and to assess the efficacy of ICSI with TESE in germinal failure. 42 non-obstructive azoospermic patients revealed no spermatozoa at all in their ejaculates, even after centrifuge. The histology of 42 patients revealed 15 Sertoli cell only Syndrome, 4 maturation arrest and 23 severe hypospermatogenesis. All patients underwent extensive multiple testicular biopsy for sperm retrieval. These patients were scheduled for ICSI using testicular spermatozoa. In 25 out of 42 non-obstructive azoospermic patients, spermatozoa were recovered from multiple testicular biopsy specimen and 11 ongoing pregnancies were achieved. There are usually some tiny foci of spermatogenesis which allow TESE with ICSI in non-obstructive azoospermia. Also these patients may have sufficient sperm in the testes for ICSI, despite extremely high FSH level and small testes.

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A Novel Multiplex-PCR Assay to Detect Three Non-Halal Meats Contained in Meatball using Mitochondrial 12S rRNA Gene

  • Cahyadi, Muhammad;Wibowo, Tommy;Pramono, Ahmad;Abdurrahman, Zakaria Husein
    • Food Science of Animal Resources
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    • v.40 no.4
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    • pp.628-635
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    • 2020
  • The objective of this study was to detect three non-halal meat products consisted of dog, pork, and rat species in meatball using novel multiplex-PCR with 12S rRNA gene as target sites. A total of 33 self-made meatballs were used, and they were grouped into eleven types of meatball based on meat species origin contained in the meatballs. Each type consisted of three meatballs. Extraction of genomic DNA from the meatballs was used as a DNA template for simplex-, duplex-, and multiplex-PCR processes. The result of simplex-PCR, duplex-PCR, and multiplex-PCR showed that the 12S rRNA primer gene successfully amplified DNA for each species bovine, dog, pig, and rat, which are respectively indicated by 155, 244, 357, and 491 bp of DNA bands. In addition, multiplex-PCR with 12S rRNA gene primers can be uniquely and accurately used for detection bovine, dog, pig, and rat species on beef meatball in one reaction.

Design of the Non-Resonant SWG Antenna with Double Slots in the Narrow Wall of Rectangular Waveguide (구형 도파관의 협벽에 이중 슬롯을 가진 비공진형 슬롯 도파관 안테나의 설계)

  • Hur, Moon-Man
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.22 no.1
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    • pp.106-113
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    • 2011
  • In this paper, the non-resonant SWG(Slotted Waveguide) antenna with double slots in narrow wall of rectangular waveguide is designed. Because energy radiated from each slot depends upon inclination angle of slot of the designed antenna, inclination angle of each slot is controlled to satisfy the amplitude distribution for required sidelobe level. Instead of the conventional extraction method of slot conductance, this amplitude distribution is made by the proposed method, which employs far-field radiation pattern calculated by Fourier transform of aperture field distribution on slot. The non-resonant double SWG antenna is designed by the proposed method and is manufactured. The antenna performances are measured and compared with the simulated results.

Output-error state-space identification of vibrating structures using evolution strategies: a benchmark study

  • Dertimanis, Vasilis K.
    • Smart Structures and Systems
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    • v.14 no.1
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    • pp.17-37
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    • 2014
  • In this study, four widely accepted and used variants of Evolution Strategies (ES) are adapted and applied to the output-error state-space identification problem. The selection of ES is justified by prior strong indication of superior performance to similar problems, over alternatives like Genetic Algorithms (GA) or Evolutionary Programming (EP). The ES variants that are being tested are (i) the (1+1)-ES, (ii) the $({\mu}/{\rho}+{\lambda})-{\sigma}$-SA-ES, (iii) the $({\mu}_I,{\lambda})-{\sigma}$-SA-ES, and (iv) the (${\mu}_w,{\lambda}$)-CMA-ES. The study is based on a six-degree-of-freedom (DOF) structural model of a shear building that is characterized by light damping (up to 5%). The envisaged analysis is taking place through Monte Carlo experiments under two different excitation types (stationary / non-stationary) and the applied ES are assessed in terms of (i) accurate modal parameters extraction, (ii) statistical consistency, (iii) performance under noise-corrupted data, and (iv) performance under non-stationary data. The results of this suggest that ES are indeed competitive alternatives in the non-linear state-space estimation problem and deserve further attention.

Classification of General Sound with Non-negativity Constraints (비음수 제약을 통한 일반 소리 분류)

  • 조용춘;최승진;방승양
    • Journal of KIISE:Software and Applications
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    • v.31 no.10
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    • pp.1412-1417
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    • 2004
  • Sparse coding or independent component analysis (ICA) which is a holistic representation, was successfully applied to elucidate early auditor${\gamma}$ processing and to the task of sound classification. In contrast, parts-based representation is an alternative way o) understanding object recognition in brain. In this thesis we employ the non-negative matrix factorization (NMF) which learns parts-based representation in the task of sound classification. Methods of feature extraction from the spectro-temporal sounds using the NMF in the absence or presence of noise, are explained. Experimental results show that NMF-based features improve the performance of sound classification over ICA-based features.

Experiments on Extraction of Non-Parametric Warping Functions for Speaker Normalization (화자 정규화를 위한 비정형 워핑함수 도출에 관한 실험)

  • Shin, Ok-Keun
    • The Journal of the Acoustical Society of Korea
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    • v.24 no.5
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    • pp.255-261
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    • 2005
  • In this paper. experiments are conducted to extract a set of non-Parametric warping functions to examine the characteristics of the warping among speakers' utterances. For this Purpose. we made use of MFCC and LP spectra of vowels in choosing reference spectrum of each vowel as well as representative spectra of each speaker. These spectra are compared by DTW to give the warping functions of each speaker. The set of warping functions are then defined by clustering the warping functions of all the speakers. Noting that male and female warping functions have shapes similar to Piecewise linear function and Power function respectively, a new hybrid set of warping functions is defined. The effectiveness of the extracted warping functions are evaluated by conducting phone level recognition experiments, and improvements in accuracy rate are observed in both warping functions.

Development of a New Non-invasive Fetal Hypoxia Diagnosis System (새로운 비관혈적 태아 저산소증 진단 방법개발에 관한 연구)

  • Lee, Jeon;Lee, Kyoung-Joung
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.55 no.11
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    • pp.495-501
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    • 2006
  • Diagnostics of unborn baby is mainly aimed at prediction and detection of occurrence of intrauterine hypoxia. Consequences resulting from fetal hypoxia appear in its heart activity. In this study, we have developed a new non-invasive system for fetal hypoxia diagnosis which provides systolic time interval(STI) parameters on the basis of analysis of electrical and mechanical heart activity together. For this we have worked on 1) the proper lead system for the acquisition of abdominal ECG, 2) the independent component analysis based signal processing and fetal ECG separation, 3) the development of a hardware which consists of an abdominal ECG amplifying module and an ultrasound module and 4) the detection of characteristic points of FECG and Doppler signal and the extraction of diagnostic parameters. The developed system was evaluated by the clinical experiments in which 33 subjects were participated. The acquired STI by the system were distributed within the ranges from the well-established invasive results of other researchers. From this, we can conclude that the developed non-invasive fetal hypoxia diagnosis system is useful.

Land cover classification of a non-accessible area using multi-sensor images and GIS data (다중센서와 GIS 자료를 이용한 접근불능지역의 토지피복 분류)

  • Kim, Yong-Min;Park, Wan-Yong;Eo, Yang-Dam;Kim, Yong-Il
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.28 no.5
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    • pp.493-504
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    • 2010
  • This study proposes a classification method based on an automated training extraction procedure that may be used with very high resolution (VHR) images of non-accessible areas. The proposed method overcomes the problem of scale difference between VHR images and geographic information system (GIS) data through filtering and use of a Landsat image. In order to automate maximum likelihood classification (MLC), GIS data were used as an input to the MLC of a Landsat image, and a binary edge and a normalized difference vegetation index (NDVI) were used to increase the purity of the training samples. We identified the thresholds of an NDVI and binary edge appropriate to obtain pure samples of each class. The proposed method was then applied to QuickBird and SPOT-5 images. In order to validate the method, visual interpretation and quantitative assessment of the results were compared with products of a manual method. The results showed that the proposed method could classify VHR images and efficiently update GIS data.

Understanding Bacterial Biofilm Stimulation Using Different Methods - a Criterion for Selecting Epiphytes by Plants

  • Bhushan, Shashi;Gogoi, Mandakini;Bora, Abhispa;Ghosh, Sourav;Barman, Sinchini;Biswas, Tethi;Sudarshan, Mathummal;Thakur, Ashoke Ranjan;Mukherjee, Indranil;Dey, Subrata Kumar;Chaudhuri, Shaon Ray
    • Microbiology and Biotechnology Letters
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    • v.47 no.2
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    • pp.303-309
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    • 2019
  • Earlier studies by our group revealed that gallic acid in phytochemicals stimulated biofilm production in epiphytes, while caffeic acid in phytochemicals inhibited biofilm production in non-epiphytes. It is well documented that antimicrobial secretion by some epiphytic bacteria inhibits non-epiphytic bacterial growth on leaf surfaces. These selection criteria help plants choose their microbial inhabitants. Calcium and iron in phytochemicals also stimulate biofilm formation and thus, may be selection criteria adopted by plants with respect to their native epiphytic population. Furthermore, the processing of leaves during phytochemical extraction impacts the composition of the extract, and therefore its ability to affect bacterial biofilm formation. Computation of the Hurst exponent using biofilm thickness data obtained from the Ellipsometry of Brewster Angle Microscopic (BAM) images is an efficient tool for understanding the impact of phytochemicals on epiphytic and non-epiphytic populations when compared to fluorescent microscopy, scanning electron microscopy, and staining techniques. To the best of our knowledge, this is the first report that uses the Hurst exponent to elucidate the mechanism involved in plant microbe interaction.

Extraction of optimal time-varying mean of non-stationary wind speeds based on empirical mode decomposition

  • Cai, Kang;Li, Xiao;Zhi, Lun-hai;Han, Xu-liang
    • Structural Engineering and Mechanics
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    • v.77 no.3
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    • pp.355-368
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    • 2021
  • The time-varying mean (TVM) component of non-stationary wind speeds is commonly extracted utilizing empirical mode decomposition (EMD) in practice, whereas the accuracy of the extracted TVM is difficult to be quantified. To deal with this problem, this paper proposes an approach to identify and extract the optimal TVM from several TVM results obtained by the EMD. It is suggested that the optimal TVM of a 10-min time history of wind speeds should meet both the following conditions: (1) the probability density function (PDF) of fluctuating wind component agrees well with the modified Gaussian function (MGF). At this stage, a coefficient p is newly defined as an evaluation index to quantify the correlation between PDF and MGF. The smaller the p is, the better the derived TVM is; (2) the number of local maxima of obtained optimal TVM within a 10-min time interval is less than 6. The proposed approach is validated by a numerical example, and it is also adopted to extract the optimal TVM from the field measurement records of wind speeds collected during a sandstorm event.