• 미생물학회지
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• 제14권1호
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• pp.36-38
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• 1976

The insecticides, Dithane-M45 and copper sulfate, were introduced in this experiments to elucidate their effects on the nodulation of soybean plant(Glycine max Meer) by Rhizobium japonicum. The nodulation activity was inhibited in accordance with increase of their concentration.

• The Plant Pathology Journal
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• 제20권1호
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• pp.13-17
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• 2004

An interaction between Legumes and Rhizobia establishes a symbiotic new organ, the nodule that supports atmospheric nitrogen fIxation. The specific communications between the microbes and legume plants are necessary for both nodulation and nitrogen fixation. Through genetic and biochemical analyses several genes playing pivotal roles in nodulation had been identified to be a receptor kinase like CALVATAl involved signal transduction for development. This emphasizes peptides as signals to be transmitted for a short or long distance transport for nodulation. In addition, a quorum sensing in rhizobia has become a focus as counterpart signal. In an attempt to reveal proteins factors and signaling molecules acting on nodulation, proteome analyses of nodule and the proteins in apoplast upon communication between Legumes and Rhizobia were performed.

• 한국작물학회지
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• 제37권1호
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• pp.16-21
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• 1992

초다 근류착생성 콩 nts 382 및 nts 246와 wild-type인 Bragg의 초기생육, 뿌리혹 형성 및 질소고정능력을 조사하였으며, 그 결과는 다음과 같이 요약된다. 1. nts 계통은 뿌리혹건물중이 현저히 높아서 파종후 31일째에는 Bragg에 비하여 2.5내지 3.5배에 달하였다. 2. nts 계통은 과도한 뿌리혹 형성 때문에 지상부 광합성산물을 많이 소모하여 지상부의 생육이 저해되었다.

• Molecules and Cells
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• 제27권2호
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• pp.129-134
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• 2009

Legume plants develop root nodules to recruit nitrogen-fixing bacteria called rhizobia. This symbiotic relationship allows the host plants to grow even under nitrogen limiting environment. Since nodule development is an energetically expensive process, the number of nodules should be tightly controlled by the host plants. For this purpose, legume plants utilize a long-distance signaling known as autoregulation of nodulation (AON). AON signaling in legumes has been extensively studied over decades but the underlying molecular mechanism had been largely unclear until recently. With the advent of the model legumes, L. japonicus and M. truncatula, we have been seeing a great progress including isolation of the AON-associated receptor kinase. Here, we summarize recent studies on AON and discuss an updated view of the long-distance control of nodulation.

• Journal of Forest and Environmental Science
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• 제28권4호
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• pp.212-219
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• 2012

An experiment was set to understand the seedlings growth and nodulation capabilities of five potentially important leguminous agroforestry tree species of Bangladesh in response to application of organic fertilizer. Study was carried out in nursery bed on the seedlings of Acacia mangium Willd., Acacia hybrid, Acacia auriculiformis A. Cunn. ex Benth, Albizia lebbeck (L.) Benth. and Leucaena leucocephala (Lam.) de Wit. after sowing their seeds in polybags filled with different medium. Farmyard manure or decomposed cow dung was applied as the source of organic fertilizer with other potting media. The growth and nodulation performance of seedlings of the selected species were measured three moths after the first seed was emerged. The different soil: organic manure ratios' used during the experiment were, 1:1, 2:1 and 3:1. The effects of organic fertilizer on growth and nodulation were compared to that of seedlings grown in control. The results revealed a positive correlation between seedling growth and the different doses of organic fertilization (i.e. seedling growth was enhanced significantly with different treatments containing higher proportion of organic fertilizer). In few cases a negative effect was observed after applying higher levels of organic manure. It was also apparent that the nodulation in terms of nodule number and size was inhibited significantly by the application of organic fertilizer.

• 한국작물학회지
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• 제46권5호
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• pp.416-419
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• 2001

Hypernodulation soybean mutant, SS2-2, is characterized with greater nodulation and nitrogen fixing ability in the root nodule than its wild type, Shinpaldalkong 2. The present study was performed to identify a genetic locus conferring hypernodulation in soybean mutant SS2-2 and to determine whether the gene controlling the hypernodulation of SS2-2 is allelic to that controlling the supernodulation of nts382 mutant. Hybridization studies between SS2-2 and Taekwangkong revealed that the recessive gene was responsible for the hypernodulation character in soybean mutant SS2-2. Allelism was also tested by crossing supernodulating mutant nts382 and hypernodulating mutant SS2-2 that both hypernodulation and supernodulation genes were likely controlled by an identical locus. Molecular marker mapping of hypernodulation gene in SS2-2 using SSR markers confirmed that the gene conferring hypernodulation was located at the same loci with the gene conferring supernodulation. It is interesting to note that the same gene controlled the super- and hyper-nodulation characters, although SS2-2 and nts 382 exhibited differences in the amount of nodulation in the root system. Further genetic studies should be needed to clarify the genetic regulation of super- and hyper-nodulation in soybean.

• 한국작물학회지
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• 제48권5호
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• pp.355-360
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• 2003

The responses of six mungbean [Vigna radiata (L.) Wilczek] varieties to Rhizobium inoculation on number and dry weight of nodules, nitrogenase activity of root nodule bacteria, dry weight of shoot and root, nitrogen content, and uptake by shoot were investigated. The mungbean varieties were BARI Mung-2, BARI Mung-3, BARI Mung-4, BARI Mung-5, BINA Moog-2, and BU Mung-1. Two-third seeds of each variety were inoculated with Rhizobium inoculant and the remaining one-third seeds were kept uninoculated. Rhizobium strains TAL 169 and TAL 441 were used for inoculation of seeds. Inoculation of seeds with Rhizobium strains significantly increased nodulation, nitrogenases activity, dry matter production, nitrogen content, and uptake by shoot of the crop compared to uninoculated control. There was positive correlation among the number and dry weight of nodules, nitrogenase activity, dry weight of shoot and root, nitrogen content, and uptake by shoot of the crop. It was concluded that BARI Mung-4 in association with Rhizobium strain TAL 169 performed best in recording nodulation, nitrogenase activity, dry matter production, and nitrogen uptake by shoot of mungbean.

• 한국작물학회지
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• 제10권
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• pp.79-83
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• 1971

뉴지랜드의 황갈색 고원 토양에 있어 Lucerne의 근류형성 및 생육에 미치는 근류접종, pelleting 및 석회시용의 효과를 시험코저 5개 수준의 종자 처리(블접종, 접종, 접종 석회 pelleting, 접종인산 백운석 50/50 pelleting, 접종활석 pelleting과 석회시용(25kg/10are)을 비교하고 그들의 상호작용을 관찰하였던바 다음과 같은 결과를 얻었다. 1. 산성토양에서 Lucerne의 효과적 근류 형성에는 석회시용과 종자의 근류균 접종 및 pelleting이 필요하다. 2. 접종 종자를 적당한 재료로 싸면 근류헝성을 좋게한다. 3. Lucerne의 종자 pelleting은 석회시용 요구량을 감소시키는 수단이 될 수 있다. 4. 창갈색 고원 토양에서는 석회분말이 좋은 pelleting재료가 된다. 5. 종자에 접종하지 않거나 pelleting을 하지 않은 종자의 파종시는 석회시용이 효과적이다. 6. pelleting 종자 사용은 석회시용에 소요되는 품과 경비를 절감시킬 것이다.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.570-579
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• 2024

Root-nodule nitrogen-fixing bacteria are known for being specific to particular legumes. This study isolated the endophytic root-nodule bacteria from the nodules of legumes and examined them to determine whether they could be used to promote the formation of nodules in other legumes. Forty-six isolates were collected from five leguminous plants and screened for housekeeping (16S rRNA), nitrogen fixation (nifH), and nodulation (nodC) genes. Based on the 16S rRNA gene sequencing and phylogenetic analysis, the bacterial isolates WC15, WC16, WC24, and GM5 were identified as Rhizobium, Sphingomonas, Methylobacterium, and Bradyrhizobium, respectively. The four isolates were found to have the nifH gene, and the study confirmed that one isolate (GM5) had both the nifH and nodC genes. The Salkowski method was used to measure the isolated bacteria for their capacity to produce phytohormone indole acetic acid (IAA). Additional experiments were performed to examine the effect of the isolated bacteria on root morphology and nodulation. Among the four tested isolates, both WC24 and GM5 induced nodulation in Glycine max. The gene expression studies revealed that GM5 had a higher expression of the nifH gene. The existence and expression of the nitrogen-fixing genes implied that the tested strain had the ability to fix the atmospheric nitrogen. These findings demonstrated that a nitrogen-fixing bacterium, Methylobacterium (WC24), isolated from a Trifolium repens, induced the formation of root nodules in non-host leguminous plants (Glycine max). This suggested the potential application of these rhizobia as biofertilizer. Further studies are required to verify the N₂-fixing efficiency of the isolates.

• 미생물학회지
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• 제35권2호
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• pp.169-172
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• 1999

한천 사면배지 대신 한지를 사용하여 시험관내 뿌리혹생성 실험방법을 개선하였다. 개선된 이 방법은 기존의 한천-시험관 법에 비해 전체 과정이 간단하며, 특히 굵고 단단한 유근을 갖는 콩과식물에서도 효과적으로 사용할 수 있다. 이 방법을 사용하여 Bradyrhizobium sp. CN9135을 접종한 차풀에서 접종후 7일째부터 조사된 식물개체의 6%에서 뿌리혹이 생기기 시작하여 14일 째에 조사된 모든 식물개체에서 뿌리혹이 생성되었다.