• Title/Summary/Keyword: Nitrogen transformation

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Effects of Carcinogens and Partial Hepatectomy on the Nitrogen Utilizing and the Xenobiotic Metabolizing Enzymes in the Hepatic Tissues of Rats (발암원과 부분간절제술 처리에 의한 백서 간 조직중 질소이용계 및 이물질 대사계 효소의 변화)

  • 박상철;김응국;곽상준;이건욱;김수태
    • Environmental Mutagens and Carcinogens
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    • v.8 no.1
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    • pp.22-34
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    • 1988
  • The biochemical changes of the hepatic tissues, induced by the carcinogen treatment such as diethylnitrosamine and acetamidofluorene in combination with the partial hepatectomy after Solt and Farber, were determined for the characterization of the induction of the proliferative capacity and the environmental adaptability of the carcinogenic tissues during the malignant transformation process. For the study of the proliferative capacity of the tissues, the activities of the enzymes, related with the nitrogen trapping mechanism, such as glutamine synthetase and gamma-glutamyltranspeptidase, were monitroed, while the cintents of cytochrome P450's and their isozymic patterns as well as the activities of the glutathione S-transferase were determined in the function of time after the hepatocarcinogenic stimuli.

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Sliding Wear Mechanism of the High-Nitrogen Austenitic 18Cr-l8Mn-2Mo-0.9N Steel (고질소 Fe-l8Cr-l8Mn-2Mo-0.9N 강의 미끄럼 마멸 기구)

  • Kim, S.D.;Kim, S.J.;Kim, Y.S.
    • Transactions of Materials Processing
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    • v.15 no.2 s.83
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    • pp.112-117
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    • 2006
  • Sliding wear mechanism of a high nitrogen austenitic 18Cr-18Mn-2Mo-0.9N steel has been investigated. Dry sliding wear tests of the steel were carried out at various loads of IN-10N under a constant sliding speed condition of 0.15m/s against AISI 52100 bearing steel balls. Solution ($1050^{\circ}C$) and isothermal aging ($900^{\circ}C$) heat treatments were performed on the steel and the effect of the heat treatments on the wear was investigated. Wear rates of the solution-treated steel specimen remained low until 5N load, and then increased abruptly at loads above 5N. The rates of isothermally aged specimens were low and increased gradually with the applied load. Worn surfaces, their cross sections, and wear debris of the steel specimens were examined with a scanning electron microscopy. Phases of the heat-treated specimen and the wear debris were identified using XRD. The transformed phase underneath a sliding track was investigated and analyzed using a TEM. Effects of the phase transformation during the wear and $Cr_{2}N$ precipitates formed during the isothermal aging on the wear of the austenitic steel were discussed.

Sliding wear mechanism of the high-nitrogen austenitic 18Cr-18Mn02Mo-0.9N steel (고질소 Fe-18Cr-18Mn-2Mo-0.9N강의 미끄럼 마멸 기구)

  • Kim S. D.;Kim S. J.;Kim Y. S.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2005.10a
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    • pp.105-108
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    • 2005
  • Sliding wear mechanism of a high nitrogen austenitic 18Cr-18Mn-2Mo-0.9N steel has been investigated. Dry sliding wear tests of the steel were carried out at various loads of 1N-10N under a constant sliding speed condition of 0.15m/s against AISI 52100 bearing steel balls. Solution $(1050^{\circ}C)$ and isothermal aging $(900^{\circ}C)$ heat treatments were performed on the steel and the effect of the heat treatments on the wear were investigated. Wear rates of the solution-treated steel specimen remained low until 5N, and then increased abruptly at loads above 5N. The rates of isothermally aged specimens were low and increased gradually with the applied load. Worn surfaces, their cross sections, and wear debris of the steel specimens were examined with a scanning electron microscopy. Phases of the heat-treated specimen and the wear debris were identified using XRD. Phases transformed underneath the sliding track during the wear were investigated and analyzed using TEM. Effects of the phase transformation during the wear and $Cr_2N$ precipitates formed during the isothermal aging on the wear of the austenitic steel were discussed.

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Influence of oxytetracycline on the fate of Nitrogen species in a recirculating aquaculture system

  • Medriano, Carl A.D.;Yoon, Hyojik;Chandran, Kartik;Khanal, Samir.K.;Lee, Jaewoo;Cho, Yunchul;Kim, Sungpyo
    • Membrane and Water Treatment
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    • v.9 no.2
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    • pp.123-128
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    • 2018
  • Common aquaculture practices include the use of certain pharmaceuticals such as antibiotics in avoiding diseases and promoting a healthier growth of the culture. The aim of this study is to monitor and assess the influence of different low oxytetracycline concentrations on the transformation of nitrogen compounds under aeration condition in a lab-scale recirculating aquaculture system (RAS). Over $1mg\;L^{-1}$ dose of oxytetracycline to aquaculture had induced ammonia($NH_4-N$), nitrate($NO_3-N$), soluble COD accumulation in RAS. In addition, nitrous oxide ($N_2O$) emission from RAS was significantly reduced during the oxytetracycline dose periods. After ceasing the dose of oxytetracycline, ammonia oxidation and nitrous oxide re-emission were observed. This observation indicated that low concentrations of oxytetracycline could affect the nitrogen species in RAS. Also, the emission mechanisms of $N_2O$ may not be only dependent on nitrification process but also dependent on denitrification process in our RAS system.

Interspecies Transfer and Regulation of Pseudomonas stutzeri A1501 Nitrogen Fixation Island in Escherichia coli

  • Han, Yunlei;Lu, Na;Chen, Qinghua;Zhan, Yuhua;Liu, Wei Liu;Lu, Wei;Zhu, Baoli;Lin, Min;Yang, Zhirong;Yan, Yongliang
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1339-1348
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    • 2015
  • Until now, considerable effort has been made to engineer novel nitrogen-fixing organisms through the transfer of nif genes from various diazotrophs to non-nitrogen fixers; however, regulatory coupling of the heterologous nif genes with the regulatory system of the new host is still not well understood. In this work, a 49 kb nitrogen fixation island from P. stutzeri A1501 was transferred into E. coli using a novel and efficient transformation strategy, and a series of recombinant nitrogen-fixing E. coli strains were obtained. We found that the nitrogenase activity of the recombinant E. coli strain EN-01, similar to the parent strain P. stutzeri A1501, was dependent on external ammonia concentration, oxygen tension, and temperature. We further found that there existed a regulatory coupling between the E. coli general nitrogen regulatory system and the heterologous P. stutzeri nif island in the recombinant E. coli strain. We also provided evidence that the E. coli general nitrogen regulator GlnG protein was involved in the activation of the nif-specific regulator NifA via a direct interaction with the NifA promoter. To the best of our knowledge, this work plays a groundbreaking role in increasing understanding of the regulatory coupling of the heterologous nitrogen fixation system with the regulatory system of the recipient host. Furthermore, it will shed light on the structure and functional integrity of the nif island and will be useful for the construction of novel and more robust nitrogen-fixing organisms through biosynthetic engineering.

Influences of Cooling Atmosphere on the Whiteness in White Portland Cement (백 시멘트의 백색도에 미치는 냉각분위기의 영향)

  • 한기성;최상흘
    • Journal of the Korean Ceramic Society
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    • v.15 no.2
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    • pp.85-88
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    • 1978
  • Influence of cooling atmosphere on the whiteness in white portland cement was studied. The effective composition of gaseous medium of cooling atmosphere is desirable less than 0.5% of $O_2$ and 0.5~1.5% of CO. When theclinker is quenched in nitrogen atmosphere, the color of clinker becomes bright pink. In a weakly reducing gaseous medium or vacuum, the absence of oxygen brings defects in crystal and transformation in the coordination of iron, and as a result, whiteness is raised.

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WC-Co coating by cold spray deposition (Cold spray를 이용한 WC-Co 코팅)

  • 김형준;황순영;권영각
    • Proceedings of the KWS Conference
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    • 2004.05a
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    • pp.311-313
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    • 2004
  • WC-12-17%Co powders with nano- and micro-structures were deposited by cold spray process using nitrogen and helium gases. The results show that there is no detrimental phase transformation and/or decarburization of WC by cold spray deposition as expected. It is also observed that nano-sized WC in the feedstock powder is maintained in the cold spray deposition. It is demonstrated that it is possible to fabricate the nano-structured WC-Co coating with low porosity and very high hardness (-2050 HV) by cold spray deposition with reasonable powder preheating.

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Changes of Soil Properties in Black Locust(Robinia pseudoacacia L.) Stand and Adjacent Stand (아까시나무(Robinia pseudoacacia L.)조림지와 인접 임분의 토양특성 변화)

  • Jung, Sung-Chul;Huh, Tae-Chul;Joo, Sung-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.21
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    • pp.39-47
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    • 2003
  • The object of this study was to analyze influence which Black locust afforestation affected to soil, to research change of soil environment of Black locust planted stand and adjacent stands through comparative analyzing physichemical property of soil of them. It was investigated that the soil texture of Black locust planted stand and the adjacent stands was mostly SL. and LS. According to this result I could know that transformation of soil texture by vegetation easily happened because transformation soil texture results from the parent rock. I could ascertain that difference existed between Black locust planted stand and the other stands in soil pH and Carbon content of planted stand of Black locust pH, contents of Available $P_2O_5$ Carbon, Total Nitrogen. In other words, pH and Carbon content of Black locust planted stand were lower than that of Oak forest, higher than that of Pine forest. These results showed that Black locust was which fix nitrogen. I could infer that Black locust afforestation made forest soil fertilized. In soil of Black locust planted stand and the adjacent stands, statistical value appeared highly in pH and existed in content of Carbon, Total nitrogen, too. And high interrelationship appeared in the order of pH, C/N Ratio, CEC, Total nitrogen content was in each stands. I suppose that the trespassing extent of Black locust to Oak forest and Pine forest which are adjacent to planted stand of Black locust is different because of low pH, lackness of Available $P_2O_5$ Carbon, and what not.

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Identification of Octopine Type Ti Plasmid in Agrobacterium tumefaciens KU12 (Agrobacterium tumefaciens KU12내에 존재하는 Octopine Type Ti Plasmid의 확인)

  • 이용욱;음진성;심웅섭
    • Korean Journal of Microbiology
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    • v.31 no.4
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    • pp.292-299
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    • 1993
  • Agrobacterium tumefaciens KU12 isolated from Korea is able to induce tumors on various plants and catabolize octopine as a sole carbon and nitrogen source. A, tumefaciens KU12 contains three plasmids. Their sizes are 45.5 kb. 240 kb. and > 240 kb. respectively. For the purpose of identification of octopine type Ti plasmid, avirulent A, tumefacients A136 is transformed with plasmids isolated from KU12 by direct transformation. Transformants containing Ti plasmid were grown on AB medium containing octopine as a sole nitrogen source. The isolated strain, named KU911, contains only 240 kb plasmid. As a result of induction of crown gall and Southern hybridization with other octopine Ti plasmid pTiAch5, 240 kb plasmid named pTiKU12 was Ti plasmid.

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A Study on the Characteristics of Martensitic Transformation Behaviors in In-X(X=Pb,Sn) Alloys (In-X(X=Pb,Sn) 합금의 마르텐사이트변태거동 특성에 관한 연구)

  • Han, Chang-Suk;Han, Seung-Oh
    • Journal of the Korean Society for Heat Treatment
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    • v.23 no.5
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    • pp.233-238
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    • 2010
  • The phase transformations and the shape memory effect in In-rich Pb alloys and In rich-Sn alloys have been studied by means of X-ray diffractometry supplemented by metallographic observations. The alloys containing 12~15 at.%Pb transform from the ${\alpha}_2$ (fct) phase to the ${\alpha}_1$ (fct) phase by way of an intermediate phase (m phase) on cooling. The results of X-ray diffraction show that the metastable intermediate phase is observed both on cooling and heating, and has a face-centered orthorhombic (fco) structure. It is concluded that the ${\alpha}_1{\rightleftarrows}{\alpha}_2$ transformation is expressed by the ${\alpha}_1{\rightleftarrows}m{\rightleftarrows}{\alpha}_2$ transformation both on usual cooling and heating with the rate more than $8{\times}10^{-3}$ K/s. The $m{\rightleftarrows}{\alpha}_2$ transformation takes place with a mechanism involving macroscopic shear and are of diffusionless (martensitic) type. The temperature hysteresis in the two transformations is 10~13 K between the heating and cooling transformations. The alloys containing 0~11 at.%Sn are -phase solid solutions with a face centered tetragonal structure (c/a > 1) at room temperature, the axial ratio increasing continuously with tin content. The In-(11~15) at.%Sn alloys are mixtures of ${\alpha}$ and ${\beta}$ phases, the ${\beta}$ phase having a f. c. tetragonal structure (c/a < 1). The alloys containing more than 15 at.%Sn are ${\beta}$-phase solid solutions. The In-(12.9~15.0) at.%Sn alloys show a shape memory effect only when quenched to the temperature of liquid nitrogen, although their effect becomes weak and finally disappears after keeping at room temperature for a long time. The ${\beta}{\rightarrow}{\alpha}^{\prime}$ phase transformation is of the diffusionless (martensitic) type, and takes place between 330 K at 12.9 at.%Sn and 150 K at 14.5 at.%Sn. The hysteresis of transformation temperatures on heating and cooling is considerably large (29~40 K), depending on the composition. Both In-Pb and In-Sn alloys showed distinct the shape memory effects.