• Journal of the Korean Society for Marine Environment & Energy
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• v.15 no.4
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• pp.314-322
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• 2012

In order to elucidate temporal variations of temperature, salinity, pH, dissolved oxygen (DO), suspended particulate matter (SPM), dissolved inorganic nutrients, and chlorophyll a, we performed 25-hour continuous monitoring in the Seomjin River Estuary in March (dry season) and July (rainy season) 2006. We also investigated spatial variations of marine environmental parameters across a saline gradient. In the Seomjin River Estuary, continuous monitoring results revealed that salinity variations were mainly affected by tidal cycle in the dry season and by river discharge in the rainy season. In the dry season, the spatio-temporal distribution of dissolved inorganic nutrient (nitrate, nitrite, and silicate) concentrations showed a good correlation with tidal cycle. While nutrient concentrations in rainy season showed not much variance in time. There were 6 and 4 times higher dissolved inorganic nitrogen and phosphorus concentrations in the rainy season than those in the dry season, respectively. Silicate concentration was 43 times higher in the rainy season than that in the dry season. Chlorophyll a concentration was higher in the dry season than that in the rainy season showing high nutrient concentrations. The results of this study, spatio-temporal variations of marine environmental factors are determined by both tidal cycle and river discharge. It seems that chlorophyll a concentration is related to the river discharge than dissolved inorganic nutrient distribution.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1562-1570
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• 2014

The effects of Lactobacillus mucosae (L. mucosae), a potential direct fed microbial previously isolated from the rumen of Korean native goat, on the rumen fermentation profile of brewers grain were evaluated. Fermentation was conducted in serum bottles each containing 1% dry matter (DM) of the test substrate and either no L. mucosae (control), 1% 24 h broth culture of L. mucosae (T1), or 1% inoculation with the cell-free culture supernatant (T2). Each serum bottle was filled anaerobically with 100 mL of buffered rumen fluid and sealed prior to incubation for 0, 6, 12, 24, and 48 h from which fermentation parameters were monitored and the microbial diversity was evaluated. The results revealed that T1 had higher total gas production (65.00 mL) than the control (61.33 mL) and T2 (62.00 mL) (p<0.05) at 48 h. Consequently, T1 had significantly lower pH values (p<0.05) than the other groups at 48 h. Ammonia nitrogen ($NH_3$-N), individual and total volatile fatty acids (VFA) concentration and acetate:propionate ratio were higher in T1 and T2 than the control, but T1 and T2 were comparable for these parameters. Total methane ($CH_4$) production and carbon dioxide ($CO_2$) were highest in T1. The percent DM and organic matter digestibilities were comparable between all groups at all times of incubation. The total bacterial population was significantly higher in T1 (p<0.05) at 24 h, but then decreased to levels comparable to the control and T2 at 48 h. The denaturing gradient gel electrophoresis profile of the total bacterial 16s rRNA showed higher similarity between T1 and T2 at 24 h and between the control and T1 at 48 h. Overall, these results suggest that addition of L. mucosae and cell-free supernatant during the in vitro fermentation of dried brewers grain increases the VFA production, but has no effect on digestibility. The addition of L. mucosae can also increase the total bacterial population, but has no significant effect on the total microbial diversity. However, inoculation of the bacterium may increase $CH_4$ and $CO_2$ in vitro.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.2
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• pp.207-212
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• 2008

This study focused on shorten the period of the struvite crystal birth and development by adding seed materials. For this purpose, three different seed materials were selected: sand, anthracite and struvite. The experiments has been conducted to evaluate the effect of the particle size of the selected seed material on the struvite crystallization, and to study the mixing effect which can be expressed by the value of $G{\cdot}t_d$(the multiple of mean velocity gradient(G) and mixing time($t_d$)). It was observed in this study that the removal efficiency of ammonia nitrogen increased by 9%, 11%, and 20% for sand, anthracite, and struvite added as the seed material, respectivley. This indicated that the struvite crystallization efficiency had a close correlation with the specific surface area of the seed particle. It was found that when struvite was selected as the seed material, the struvite crystallization proceeded at lower $G{\cdot}t_d$ value as compared with other seed materials. This observation implied that the secondary crystal birth would be dominated in this reaction. It was concluded in this study that the particle size was not significant factor on the struvite crystallization, while the $G{\cdot}t_d$ value was a considerably important factor in terms of the theory of the struvite crystal birth.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.3
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• pp.309-319
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• 2011

We experimentally investigated lifted propane jet flames diluted with nitrogen to obtain flame-stability maps based on heat-loss-induced self-excitation. We found that heat-loss-induced self-excitations are caused by conductive heat loss from premixed flame branches to trailing diffusion flames as well as soot radiation. The conductive-heat-loss-induced self-excitation at frequencies less than 0.1 Hz is explained well by a suggested mechanism, whereas the oscillation of the soot region induces a self-excitation of lift-off height of the order of 0.1 Hz. The suggested mechanism is also verified from additive experiments in a room at constant temperature and humidity. The heat-loss-induced self-excitation is explained by the Strouhal numbers as a function of the relevant parameters.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.1
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• pp.86-96
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• 2002

The distributions of suspended particulate matter (SPM), chlorophyll a, particulate organic carbon (POC) and nitrogen (PON) and particulate biogenic silica (PBSi) along the salinity gradient were investigated in the Seomjin River estuary from March 1999 to April 2001. Sampling sites were set based on the surface salinity during each cruise rather than geographic locations. Concentrations of SPM were less than 20 mg/L, suggesting relatively low input of terrestrial SPM despite large freshwater discharge through Seomiin River, Chlorophyll a peaks occurred at 5$\~$ 15 psu salinity zone (10$\~$20 km from Nan Cho Island) in November 1999, at 15$\~$25 psu (10$\~$20 km) salinity zone in April 2000 and at 1$\~$15 psu salinity zone (15$\~$20 km) in October 2000 (ca. 8$\~$58%\mu$g/L). Concentrations of POC, PON and PBSi were also high at the same zone. Relatively low ratios of POC to chlorophyll a in mid-salinity zone where POM peak occurred suggests high contribution of living phytoplankton to the total POC. On the other hand, relatively high ratios of POC to chlorophyll $\alpha$ in very low salinity zone and the mouth of estuary indicated relatively high portions of detrital POC. Consequently, the low concentrations of SPM in this estuary and the high concentrations of chlorophyll $\alpha$ and the low ratios of POC to chlorophyll $\alpha$ in the mid-salinity zone suggest that production of living phytoplankton is primary factor in controlling distribution of POM along the salinity gradients in the Seomjin River estuary.

• Korean Journal of Agricultural Science
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• v.20 no.2
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• pp.145-152
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• 1993

Vegetational data obtained from 15 quadrats of Mt. Daeam area were analysed by applying two multivariate methods : two-way indicator species analysis(TWINSPAN) for classification and detrended canonical correspondence analysis(DCCA) for ordination. The dominant tree species were Quercus mongolica, Acer pseudo-sieboldianum, Betula costata, and Acer mono in descending order. The pH ranges were 5.0 to 5.5 in the 15 sites near bog and 5.3 to 5.4 in the 3 sites within bog. The forest communities in Mt. Daeam were classified into Quercus mongolica-Acer mono, Quercus mongolica-Prunus sargentii, Quercus mongolica-Abies nephrolepis-Acer tschonoskii var, rubripes, and Quercus mongolica-Abies nephrolepis-Betula costata according to the TWINSPAN. The relationships between the distribution of dominant species of forest vegetation and soil in the forest of Mt. Daeam were investigated by analyzing soil nutrition gradient. Quercus mongolica-Prunus sargentii forests were distributed in the good sites in nutrition levels of total nitrogen, cation exchange capacity, $Mg^{+{+}}$ and $Ca^{+{+}}$ but Quercus mongolica-Abies nephrolepis-Betula costata forests in the poor sites in the nutrition levels.

• Journal of the Korean earth science society
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• v.22 no.6
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• pp.480-490
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• 2001

The soil-air exchange processes of Hg were investigated from the Hari area of Kang Hwa Island during the late March 2001. In the course of our study, we determined the concentration gradients of Hg and combined these gradient data with micrometeorological components to derive its fluxes. Results of our study indicate that the concentration levels of Hg in the study area are notably lower than those typically found in urbanized areas of Korea. However, the computed fluxes were seen to be significantly larger for relatively remote areas, reaching over 200 ng/m$^2$/hr. Comparison of environmental conditions shows that the concentrations of most pollutant species including NO$_X$ and PM were significantly higher during emission, while meteorological conditions were characterized by high temperature and low humidity. Results of correlation analysis also indicate that such pollutants as hydrocarbons, nitrogen oxides, and PM generally exhibit strong positive correlations with Hg-related parameters during emission events, while such relationships were reversed during dry deposition events. The results of our present study suggest the possibility that the concentrations and fluxes of total gaseous Hg observed during deposition events can be influenced by the processes that are also important for the fine, rather than coarse, size fraction of particles.

• 한국해양학회지
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• v.25 no.1
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• pp.8-20
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• 1990

A series of pore water data were obtained during the different time over one year period between October 1987 and October 1988, from a site on a muddy intertidal flat, located in the Kyeong-gi Bay, west coast of Korea, The results have revealed that the tidal flat is an environment of active nutrient the subface supplied by the overlying seawater is almost completely removed from the pore water at depth of about 10 cm below the sediment surface. The nutrients such as ammonium and phosphate are produced through this process and subsequently accumulated in the pore water forming steep gradients near the sediment surface. Below the main sulfate redirection zone, a secondary peak of dissolved sulfate was often observed. Greal seasonal variation of the pore water nutrient profiles was observed, which was particularly clear in their maximum concentration as well as in their concentration gradient. The rate constants of sulfate reduction and nutrient regeneration, estimated by using a diagenetic model (Berner, 1980), differ by an order of magnitude between the summer and winter seasons. The difference in sediment temperature may account for most of the calculated variation. The C:N:P ratio, calculated from the pore water nutrient gradients also exhibits a slight seasonal difference. The organic matter being decomposed by sulfate reduction appears to be depleted in depleted in nitrogen, compared to the average marine organic matter.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.sup3
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• pp.179-183
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• 2016

Breast cancer is the most prevalent type of cancer among women around the world, and mortality is primarily caused by micro-metastatic disease. The complex mechanisms of breast cancer invasion and metastasis are intrinsically related to the malignant cell type so that early detection of micro-metastases can help prolongation of survival for patient. The aim of the present research work was evaluation of the expression status of mammoglobin protein as a candidate molecular marker in the negative sentinel lymph node (SLN). Fifty tumor specimens, and 50 normal adjacent breast tissue samples from the same patients were selected on the basis of having more than 10% tumor content for RNA extraction from SLNs. Tumor samples and normal adjacent breast tissue were archived in the form of frozen fresh tissue in liquid nitrogen. Real-time PCR was performed on a Bioner life express gradient thermal cycler system. Mammoglobin gene overexpression in breast cancer metastasis was investigated. Single marker results were mammaglobin 66.7% and CK19 50.0%, with 58.3% for the two in combination. Due to improved outcome with at least 3 genes (83.3%), it seems, triple marker evaluation will be most likely useful for detecting micro-metastases instead of studying separate genes.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.2
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• pp.132-136
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• 2011

The Temporal variability in the food chain structure of bacteria in the sedimentary organic matter was investigated using stable isotope and fatty acid. Potential organic matter sources (Land plant, Marine POM, benthic microalgae, Riverine POM), sedimentary organic matter and bacteria were sampled in Gamo largoon and Nanakita estuary. The main objective of the present study was to determine food sources of bacteria along with temporal variability. Land plant (${\delta}^{13}C$ = -26.6‰ and ${\delta}^{15}N$ = 3.6‰) and Riverine POM (${\delta}^{13}C$ = -25.5‰ and ${\delta}^{15}N$ = 8.9‰) were isotopically distinct from benthic microalgae (${\delta}^{13}C$ = -16.3‰ and ${\delta}^{15}N$ = 6.2‰) and Marine POM (${\delta}^{13}C$ = -20.3‰ and ${\delta}^{15}N$ = 10.3‰). ${\delta}^{13}C$ values of sedimentary organic matter showed a distinct gradient in the range of -20.7‰ to -191‰. The stable carbon and nitrogen isotope values of bacteria in the study were -20.8‰ to -18.6‰ for ${\delta}^{13}C$ and 6.5‰ to 8.6‰ for ${\delta}^{15}N$. From this results based on stable isotope measurements showed that in the bacteria was found to be dominated by Marine POM and Benthicmicoralge during 0 to 20 day. Whereas, terrestrial plant and riverine POM showed little in fluence to bacteria during the experiment.