• Title/Summary/Keyword: Nitrogen fixation

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Characteristics of Growth Response and Nitrogen Fixation of Meiilotus suaveolens Seedlings treated with Al (알루미늄 독성에 의한 전동싸리 유식물의 질소고정과 생장반응의 특성)

  • 박태규;송승달
    • Journal of Environmental Science International
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    • v.7 no.2
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    • pp.141-148
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    • 1998
  • Effects of alumlnlum(AA), soil pH and calcium(Cal on growth response and heavy metal accumulation and regulation of nitrogen fixation In Melilotus suaveolens seedlings, a biennial legume plant dominating in the disturbed area, were quantitatively analyzed during the growing periods. Accumulation of metals In each organ of M. suaveojens was Increased UC the lowering of pH. Al contents In leaf and root treat- ed with 30ppm Al at pH 4.2 on the 28th day after treatment were 8 and 11 folds higher than those of control, while the contents with 100ppm AA were 21 and 24 folds as compared to control. The significent inhibition in Al contents was induced by 100ppm AA and Ca at pH 6.5. Increased metals inhibited higher at pH 4.2 and the growth inhibition due to Al was reversed by the addition of Ca, suggesting that growth correlates to the pH value. Chlorophyll contents in leaves increased during growth stave were inhibited by Al treatments. The biomass was decreased UD the lowering of pH and the increase of concentrations. 100ppm Ca treatment resulted in 5.1-5.9% increase of the biomass as compared to that of 100ppm Al. Specific nitrogen fuation activities In nodules In the 100ppm Al at pH 4.2 and pH 6.5 were reduced to 35. 2% and 52.2% of control, on the 28th day after treatment, while the combination of Al and Ca induced reduced by 10% as compared to control with the treatment of 100ppm AA at pH 4.2 due to the inhibition bination of 100ppm Al and Ca and lowering of pH.

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Studies on Symbiotic Nitrogen Fixation in Soybeans -III. Effects of Soil Improvement on N2 Fixation and Yield of Soybean Inoculated with Rhizobium japonicum in Newly Reclaimed Upland Soil (대두근류균(大豆根瘤菌)의 질소고정(窒素固定)에 관한 연구 -III. 신개간지(新開墾地) 토양에서 근류균(根瘤菌)의 접종(接種) 및 개량제시용이 질소고정(窒素固定)과 대두수량에 미치는 효과)

  • Ryu, Jin-Chang;Lee, Sang-Kyu;Lee, Hyuk-Ho;Hong, Chong-Woon;Cho, Moo-Je
    • Korean Journal of Soil Science and Fertilizer
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    • v.16 no.2
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    • pp.188-194
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    • 1983
  • A Pot experiment was conducted to find out the effects of NPK fertilizers and soil amendments on the symbiotic nitrogen fixation and yield of soybean inoculated with Rhizobium japonicum in newly reclaimed upland soil with very low fertility. The results are summarized as follows; 1. Application of PK fertilizer and soil amendments such as lime, and rice straw in combination with micronutrients (Zn, B, Mo) increased the amounts of symbiotic $N_2$ fixation in soybean. 2. The inoculation of Rhizobium japonicum with application of soil amendments increased the yield of soybean by 4% to 10% due to enhanced $N_2$ fixation. 3. In case of improvement of newly reclaimed hilly soil in order to obtain the high yield of soybean, simutaneous application of soil amendments with inoculation of R. janconican should the highest yield.

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Studies on the fluctuation of aerobic free-living nitrogen fixation bacteria in soil beneath the plant covers (식피별 비공생성 호기성 질소고정세균의 변동에 관하여)

  • 이태우;심재국
    • Korean Journal of Microbiology
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    • v.21 no.2
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    • pp.71-78
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    • 1983
  • The number of aerobic free-living nitrogen fixation bacteria and factors in soil at different stands covered with Pinus rigida, Quercus acutissima and Zoysia japonica in Cheongju area were investigated from Feb. to Sept 1981. 1. The numbers of $N_2-fixation$ bacteria, according to the seasonal changes, increased gradually from winter to spring and summer. But the growth pattern revealed some differences in accordance with plant cover stands : the numbers increased abruptly in May at Pinus, May-June at Quercus and Apr. May at Zoysia stand. The pick of numbers represented in Aug. Sept, at Pinus, Jul-Aug. at Quercus and May-Jun. at Zoysia stand, respectively. 2. The interrelationship between the monthly changes of enviotnmental factors and numbers of $N_2-fixation$ bacteria at different stands, mainly depends upon the soil temperature than other soil factors (r=0.71-0.84). The numbers of $N_2-fixation$ bacteria may increase 5-7 times according to increase $10^{\circ}C$ of soil temperature, and optimal range was $20{\sim}30^{\circ}C$ for growth. Equation of the interrelation between soil temperature and numbers could be stated as follows : log y=ax+b. 3. In the case of high soil temperature, the bacterial numbers presented high level in drought periods. Therefore, the $N_2-fixation$ bacterial species in these soil seem to consist of resistant to desication. 4. The influence of soil organic matter for growth of $N_2-fixation$ bacteria indicated low conrelation. The reason may seen the content of organic matter in these soil existed abundantly above the quantities of limitation for growth. 5. In artifical gradients, the $N_2-fixation$ bacteria were predominated at $20{\sim}30^{\circ}C$ same as natural condition, pH7-8, and 20-30% of soil water contents. 6. The vertical distribution of bacteria marked decreasing trends from surface to lower layers, and the decreasing degree was shown well in Zoysia, Quercus and Pinus stand in order. But in the trees, the numbers increased at 30cm layer estimated the region of root than 20cm layer. 7. Both catalase megative and positive group of $N_2-fixation$ bacteria in soil increased according to the rise of the soil temperature. Catalase positive group was revealed as dominant group in winter, and catalase negative group revealed in summer. The change of dominant pattern was shown during Feb. to Apr.

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Effect of mixed sowing treatment of green manure crops on the change of soil nitrogen amount and yield production of corn

  • Shim, Kang Bo;Kim, Min Tae;Kim, Sung Gook;Jung, Kun Ho;Kim, Chung Guk;Lee, Jae Un;Kwon, Young Up
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.318-318
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    • 2017
  • This study was conducted to find optimum mixed sowing ratio of green manure crops to reduce the use of chemical fertilizer as well as to increase the crop yield potential which will foster the utilization of green manure crops in the upland field in view of environment friendly agriculture. According to the study, the mixed ratio, 50:50, of hairy vetch and green barley showed highest nitrogen production yield in the soil due to the relatively higher organic nitrogen supply from the hairy vetch plant as well as nitrogen fixation from the air rather than other mixed ratio. In the 50:50 mixed ratio of hairy vetch and green barely total nitrogen amount in the soil showed 17.2kg per 10a, but in the other treatment ratio such as 75:25, 25:75. total nitrogen fixation amount were 16.7, 16.9 respectively. We also conducted the experiment to compare the effect of the mixed sowing treatment of green manure crops on the production of corn cultivated as a succeeding plant of hairy vetch. According to the result, the mixed ratio, 50:50, of hairy vetch and green barley treatment showed highest yield potential of corn as 153kg per 10a in seed weight which is due to the relatively higher organic nitrogen supply from the hairy vetch plant as well as nitrogen fixation from the air rather than other mixed ratio. In the mixed sowing treatment of hairy vetch 100 and barley 0 ratio, the corn production showed 148kg per 10a which is 5kg lower than that of hairy vetch 50 and barley 50 ratio, but showed statistically no difference between those two treatment. Otherwise, different treatments, such as hairy vetch 75 and barley 25, 25 and 75, 0 and 100 showed statistically different each other. Therefore, it was concluded that green manure crops, such as hairy vetch, green barley and rye were very effective crops to increase the soil fertility and gave the positive effect to the crops to give vegetative and propagative growth condition and, in turn, increased the yield potential. We have to make policy to enhance the utility of green manure crops in the upland crops as well as faddy field for the soil fertility and crop yield production which will guarantee prominent quality of environment friendly agriculture products.

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Study on Simulation of Runoff and Nitrogen for Application of LM3V Model in South Korea (LM3V 지면모델의 국내 적용성 평가를 위한 유출량 및 질소 모의 연구)

  • Jung, Chung Gil;Kim, Seong Joon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.59 no.4
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    • pp.1-15
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    • 2017
  • Eutrophication of surface waters is of concern worldwide, because it can result in many undesirable water-quality and ecological problems, such as hypoxic 'dead' zones and harmful algal blooms, both associated with considerable economic costs. In this study, we used LSM (Land Surface Model) to simulate nitrogen in five major rivers in the Southern Korean Peninsula. The main objective of this research was to enhance nitrogen data for input of LM3V model in South Korea. Input data for nitrogen fluxes were categorized into three sections including agriculture fertilizer, livestock manure, atmosphere deposition, biological fixation, and sewage pollutants were used as the nitrogen input. For using LM3V model, the nitrogen input data were regenerated by considering states of agriculture and industry in South Korea at a $1/8^{\circ}$ resolution. Then, we simulated stream/river flows and N loads throughout the entire drainage networks in South Korea at a $1/8^{\circ}$ resolution. By using the same parameters for the entire country ($100,210km^2$), composed of 5 river basins with varying climate and land use, the model simulates spatial (11 sites) and temporal (1999~2010) patterns of flows and nitrate-N loads are resonable by comparing observed flow and nitrate-N loads. The r (Pearson's linear correlation) for water temperature, flow and nitrate-N at river were 080~0.93, 0.62~0.92 and 0.5~0.9 respectively. Based on enhanced N input data and model results, we find that LM3V model as land surface model can be applied in South Korea with interaction of atmosphere and land conditions.

Interactive Effects of UV-B and Pesticides on Photosynthesis and Nitrogen Fixation of Anabaena doliolum

  • Chandrai, Lar;Vandana, Pandey
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.423-430
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    • 2002
  • The effects of UV-B and commercial grade pesticides (butachlor and carbofuran), individually and in combination, were studied on a variety of physiological processes of rice field cyanobacterium Anabaena doliolum. Butachlor was found to be $2-12\%$ more toxic than carbofuran and $4-24\%$ than UV-B on the growth, photosynthesis, lipid peroxidation, membrane permeability, and nitrogenase activity of the test cyanobacterium. Of the three photosynthesis inhibitors, the butachlor-induced inhibition of whole chain was approximately 3 and $21\%$ higher than carbofuran and UV-B, respectively. Although the interaction of the stress factors caused a significant inhibition (P<0.01), it was still less than the additive effect on the parameters investigated, except for PSI.

Nitrogen Fixation and In Situ Dry Matter and Fibre Constituents Disappearance of Wheat Straw Treated with Urea and Boric Acid in Murrah Buffaloes

  • Dass, R.S.;Mehra, U.R.;Verma, A.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.8
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    • pp.1133-1136
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    • 2000
  • Wheat straw was treated with 4 per cent urea at a moisture level of 50 per cent alongwith different levels of boric acid viz. 1, 2, 3 and 4 per cent, under laboratory conditions to know the impact of boric acid on ammonia-N fixation in the straw. Murrah buffaloes were used for determining the disappearance of dry matter, CP and fibre constituents by nylon bag technique. Ammoniation increased CP content of wheat straw, which increased further due to addition of boric acid. Low level of boric acid (1%) had no adverse effect on fibre constituents disappearance but at higher levels there was a depressioon in the disappearance of fibre coonstituents. It can be concluded that low level of boric acid was sufficient to trap the excess ammonia released during urea ammoniation of wheat straw without affecting other constituents and their disappearance in the rumen of buffaloes.

Estimation of Material Budget in Okutama Forest Area from Satellite Images

  • Ito, Hisao;Ogawa, Susumu
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.476-478
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    • 2003
  • Capability of material fixation in forest was noticed since COP3, but now it was difficult to understand material fixation in wide area. In this study, we attempted to estimate and test the amount of carbon, nitrogen, and phosphorus fixed by forest from satellite images. First, we classified into tree species and estimated the number of trees in the forest by species, area, and digital numbers. We inspected to apply it in wide area. Next, we compared the amount of carbon, nitrogen, and phosphorus with NDVI and each band of satellite images.

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