• Asian-Australasian Journal of Animal Sciences
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• 제22권5호
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• pp.643-650
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• 2009

Two metabolism trials were conducted with 24 wether lambs to investigate the effects of feeding crab meal and other protein supplements on N utilization, digestibility and Ca and P balance in sheep. The lambs (avg. BW, 25 kg) were randomly allotted to eight diets in each of two trials. The supplements were: i) none, negative control (NC); ii) soybean meal (SBM), control; iii) supplement based on industrial byproducts of both plant and animal origin (IPA); iv) experimental supplement based on byproducts of animal origin (ESA); v) hydrolyzed supplement No 4. (HESA); vi) commercial supplement based on animal protein (CS), $Pro-Lak^{(R)}$ vii) crab meal (CM); and viii) urea (U). The supplements supplied 33% of the total dietary N (CP, 9.8%; DM basis). Lambs fed the NC diet had lower (p<0.05) DM and OM digestibility. Lower (p<0.05) apparent absorption of N was recorded for the lambs fed the HESA and NC diets. Sheep fed CM had lower Ca absorption compared to SBM. Highest (p<0.05) P absorption was observed for lambs fed CS and CM and lowest for U and NC diets. Sheep fed CM had higher (p<0.05) total VFA concentration (65.7 ${\mu}mol/ml$), compared to those fed ESA, CS, and NC diets (47.3, 49.8, and 49.5 ${\mu}mol/ml$, respectively). Highest (p<0.05) ruminal $NH_3$ N (29.6 mg/dl) was observed in lambs fed the U diet, while those fed the NC diet had the lowest (p<0.05) average value (7.66 mg/dl). Lambs fed the U diet had the highest (p<0.05) blood urea N (10.67 mg/dl). The present study showed that N utilization of diets supplemented with experimental supplements based on feather meal and blood meal; commercial supplement based on animal protein, $Prolak^{(R)}$ supplement based on plant protein and blood meal; and crab meal are comparable with that of soybean meal.

• 한국식품영양과학회지
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• 제22권4호
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• pp.367-373
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• 1993

본 연구는 15~16세의 여자 고등학생 7명을 대상으로 4주간 평상시와 같은 생활양식과 식생활 환경하에서 에너지 섭취량(GE)과 체내 에너지 보류량(BE)의 변동을 측정하므로써 (에너지 평형법) 에너지 소비량을 산출하였다. 에너지 섭취량과 대변으로의 에너지 손실량(FE)은 열량계로 측정하였고 소변으로의 에너지 손실량 (UE)은 질소 배설량으로부터 환산하였다. 체내 지방조직량 (FM)은 피부두께를 측정하여 산출한 신체 밀도법에 의하였다. 총 에너지 섭취량에 대한 당질, 단백질 및 지방질의 구성비는 각각 70.1$\pm$1.8%, 12.2$\pm$0.7% 및 17.7$\pm$2.0% 이었다. 총 에너지 섭취량에 대한 대변으로의 에너지 손실율은 2.8%이었다. 대변과 소변으로의 에너지 손실량을 감하여 산출한 1일 1인당 평균 대사에너지량(ME)은 2022 $\pm$50㎉ 이었다. 28일동안의 체성분 변동량으로부터 산출한 체내 총 에너지변동량(BE)은 평균 2400$\pm$950㎉ 가 증가하였다. 1일 1인당 평균 에너지 소비량은 1958$\pm$87㎉로서 체중 kg당 39$\pm$2㎉ 이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권1호
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• pp.46-54
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• 2014

This study was conducted to investigate the effects of eucalyptus (E. Camaldulensis) crude oils (EuO) supplementation on voluntary feed intake and rumen fermentation characteristics in swamp buffaloes. Four rumen fistulated swamp buffaloes, body weight (BW) of $420{\pm}15.0$ kg, were randomly assigned according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. The dietary treatments were untreated rice straw (RS) without EuO (T1) and with EuO (T2) supplementation, and 3% urea-treated rice straw (UTRS) without EuO (T3) and with EuO (T4) supplementation. The EuO was supplemented at 2 mL/h/d in respective treatment. Experimental animals were kept in individual pens and concentrate mixture was offered at 3 g/kg BW while roughage was fed ad libitum. Total dry matter and roughage intake, and apparent digestibilites of organic matter and neutral detergent fiber were improved (p<0.01) by UTRS. There was no effect of EuO supplementation on feed intake and nutrient digestibility. Ruminal pH and temperature were not (p>0.05) affected by either roughage sources or EuO supplementation. However, buffaloes fed UTRS had higher ruminal ammonia nitrogen and blood urea nitrogen as compared with RS. Total volatile fatty acid and butyrate proportion were similar among treatments, whereas acetate was decreased and propionate molar proportion was increased by EuO supplementation. Feeding UTRS resulted in lower acetate and higher propionate concentration compared to RS. Moreover, supplementation of EuO reduced methane production especially in UTRS treatment. Protozoa populations were reduced by EuO supplementation while fungi zoospores remained the same. Total, amylolytic and cellulolytic bacterial populations were increased (p<0.01) by UTRS; However, EuO supplementation did not affect viable bacteria. Nitrogen intake and in feces were found higher in buffaloes fed UTRS. A positive nitrogen balance (absorption and retention) was in buffaloes fed UTRS. Supplementation of EuO did not affect nitrogen utilization. Both allantoin excretion and absorption and microbial nitrogen supply were increased by UTRS whereas efficiency of microbial protein synthesis was similar in all treatments. Findings of present study suggested that EuO could be used as a feed additive to modify the rumen fermentation in reducing methane production both in RS and UTRS. Feeding UTRS could improve feed intake and efficiency of rumen fermentation in swamp buffaloes. However, more research is warranted to determine the effect of EuO supplementation in production animals.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1580-1586
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• 2007

The objective of this study was to compare the bioefficacy of L-lysine sulfate relative to L-lysine${\cdot}$HCl for 10 to 20 kg pigs. Two experiments were conducted to determine the bioefficacy of the two sources of lysine using daily gain, feed conversion, plasma urea nitrogen and nitrogen retention as the response criteria. In experiment 1, 168 crossbred barrows ($Landrace{\times}Large$ White), weaned at $28{\pm}3$ d ($9.07{\pm}0.78$kg body weight), were allotted to one of seven dietary treatments in a $2{\times}3$ (two lysine $sources{\times}three $ lysine levels) factorial arrangement of treatments with an added negative control treatment group. The basal diet was based on corn, peanut meal and soybean meal and provided 0.67% lysine. The basal diet was supplemented with 0.1, 0.2 or 0.3% lysine equivalents supplied from either L-lysine sulfate or L-lysine${\cdot}$HCl. Each treatment was fed to six pens of pigs with four pigs per pen. The trial lasted 21 days. The relative bioefficacy value of lysine in L-lysine sulfate using daily gain, feed conversion and plasma urea nitrogen as response criteria was 1.01, 1.05 and 1.04 of the lysine in L-lysine${\cdot}$HCl, respectively. In experiment 2, 42 crossbred ($Landrace{\times}Large$ White) pigs ($16.03{\pm}1.58$ kg body weight) were housed in stainless steel metabolism cages for 10 d and fed the seven diets used in the nitrogen-balance trial. The relative bioefficacy value of L-lysine sulfate was estimated to be 0.95 as effective as L-lysine${\cdot}$HCl for nitrogen retention on an equimolar basis. The t-test analysis revealed that bioefficacy of lysine in L-lysine sulfate was not significantly different from lysine in L-lysine${\cdot}$HCl, which was set at 1.00. In conclusion, L-lysine sulfate can be used instead of L-lysine${\cdot}$HCl to fortify lysine-deficient diets fed to 10 to 20 kg pigs.

• 한국토양비료학회지
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• 제27권2호
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• pp.98-104
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• 1994

1989~'93년(年)까지 5년(年)동안 답전수환시(畓田輸換時) 작부체계(作付體系)에 따른 토양(土壤) 층위별(層位別) 양분분포(養分分布)와 양분수지(養分收支)를 검토(檢討)한 결과(結果)는 아래와 같았다. 1. 질산태질소(窒酸態室素), 치환성가리(置換性加里) 및 유효인산(有效燐酸) 함량(含量)과 EC는 표층(表層) 0~20cm에서 약간 높았을뿐 토층하부(土層下部)로 이동집적(移動集積)이 적었고, 작부체계(作付體系)에 따른 표토중(表土中) 함량은 전이환(田轉換) 감자-배추>2년 수환(輸換) 감자-배추>전전환(田轉換) 대두(大豆)>수도연작구(水稻連作區) 순(順)으로 높아 처리별(處理別) 시비량을 반영(反影)하였다. 2. 치환성(置換性) 석회(石灰)와 고토(苦土) 함량 및 pH는 심토(深土)로 갈수록 높아졌고, 이러한 경향은 특히, 수도연작구(水稻連作區)에서 현저(顯著)하였다. 작부체계(作付體系)에 따라서는 전(全) 토층(土層)에서 전전환(田轉換) 대두(大豆)>전전환(田轉換) 감자배추>2년(年) 윤환(輪換) 감자-배추>수도연작구(水稻連作區) 순(順)이었고 처리별 식물체 흡수량(吸收量) 차이가 크게 영향하였다. 3. 전전환(田轉換) 감자-배추구(區)에서 질소(窒素), 인산(燐酸), 가리(加里)의 양분수지(養分收支)는 투입량(投入量)보다 탈취량(奪取量)(흡수량)이 각각(各各) 21.5, 26.8, 9.2kg/10a 많았으나 투입량(投入量)중 화학비료량(化學肥料量)은 질소(室素)와 가리(加里)의 경우 탈취량(奪取量)에 비해 적었던 반면 인산(燐酸)은 많았다. 4. 전전환(田轉換) 대두구(大豆區)의 양분수지(養分收支)는 질소(窒素), 인산(燐酸), 가리(加里) 각각(各各) -12.8, 4.1, -1.0kg/10a로 질소(窒素)와 가리(加里)의 투입량(投入量)이 탈취량(奪取量)보다 적었으나 질소(窒素)는 근류균(根瘤菌)의 질소고정(窒素固定)으로 결핍되지 않았던 반면 가리(加里)는 생육중기(生育中期)에 결핍증상(缺乏症狀)을 나타내었다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1487-1494
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• 2003

Studies were conducted with intact White Leghorn roosters to determine the true metabolisable energy (TME) and the true amino acid availability (TAAA) in five dehulled and hulled soybean meals produced in China. 60 roosters, kept in individual cages, were fasted for 48 h and then tube-fed 50 g of one of experimental feedstuffs and their excreta was then collected for the subsequent 48 h period. Two separate collection periods were used with each meal being fed to 12 roosters. The birds were given a 15-day recovery period between collection periods. An additional 12 roosters were either fasted or fed a protein-free diet in order to estimate the extent of endogenous losses of energy and amino acids in excreta. The average values per bird for nitrogen loss, endogenous energy losses (EEL) and endogenous energy losses corrected to zero-nitrogen balance ($EEL_n$) were found to be 0.74 g, 47.0 kJ and 21.5 kJ, respectively. It was found that the TME and TAAA values of dehulled soybean meal were higher than those of hulled soybean meal. The TME and nitrogen-corrected TME metabolisable energy values of dehulled soybean meal were 10.58 and 10.74 MJ/kg, respectively, while the corresponding values for hulled soybean meal were 10.03 and 10.27 MJ/kg, respectively. The average indispensable and dispensable amino acid availability of dehulled soybean meal was 92.1 and 93.5%, compared with 89.3 and 91.4% for hulled soybean meal. Dehulled soybean meal would therefore appear to be superior to hulled soybean meal as a source of protein and energy for use in poultry rations.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1610-1616
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• 2006

Influence of different levels of corn steep liquor (CSL) on chemical composition of urea treated wheat straw (UTWS), ruminal characteristics, digestion kinetics, nitrogen (N) utilization, and nutrient digestibility by ruminally-cannulated buffalo bulls was studied in a $4{\times}4$ Latin Square Design. The CSL was used to ensile 5% UTWS at the rate of 0, 3, 6, and 9% on a dry matter (DM) basis. Total N and neutral detergent fibre (NDF) were increased with increasing level of CSL. Increased NDF content was attributable to increased neutral detergent insoluble nitrogen. Four diets were formulated to contain 20% concentrate and 80% UTWS ensiled with 0, (control), 3 (CSL3), 6 (CSL6), and 9% CSL (CSL9). All diets were mixed daily and fed at 1.5% of body weight twice daily. Ruminal NH3 concentration decreased with level of CSL used to ensile UTWS at 3 and 6 h post prandial, however, at 9 h post parandial it was similar across all diets and at 12 h post prandial was higher with diets containing UTWS ensiled with CSL. Concentrations of total ruminal volatile fatty acid and acetate were increased with the CSL level used to ensile UTWS. Increased rate of disappearance and reduction in lag time of DM and NDF was recorded with diets containing UTWS ensiled with CSL. Dry matter and NDF digestibilities were higher with CSL diets than on the control diet. Buffalo bulls retained more N with diets containing UTWS ensiled with CSL. The present results indicated that UTWS could be ensiled with CSL to improve its nutritive value and N utilization by ruminants.

• 한국토양비료학회지
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• 제46권6호
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• pp.593-598
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• 2013

The livestock manure is a good source of major plant nutrients such as nitrogen, phosphorus and potassium and micronutrient that plants require. As aspect of establishment of nutrient management policy in nation scale, the use of livestock manure for agricultural purpose is a key factor for improving national nutrient balance. In this study, we evaluated the tendency of livestock manure production and amount of chemical fertilizer used in Korea from 1990 to 2011. The number of pig and chicken has been increased about two times in 2011 compared with 1990 and therefore livestock manure continually increased with the lapse of year, but cattle and dairy was not changed significantly. The chemical fertilizer consumption of nitrogen, phosphorus and potassium per hectare arable land was decreased by 54.6, 66.0 and 63.2% in 2011 compared with 1990, respectively. In contrast, the potential input of nitrogen, phosphorus and potassium to arable land through livestock manure was increased by 220, 210 and 210% during this period, respectively. Therefore we need a reasonable strategy for nutrient management such as combination of both manure and chemical fertilizers in near future.

• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.113-128
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• 1999

Protozoa can represent as half of the total rumen microbial biomass. Around 10 genera are generally present on the same time in the rumen. Based on nutritional aspects they can be divided in large entodiniomorphs, small entodiniomorphs and isotrichs. Their feeding behaviour and their enzymatic activities differ considerably. Many comparisons between defaunated and refaunated animals were carried out during the last two decades to explain the global role of protozoa at the ruminal or animal levels. It is now generally considered that a presence of an abundant protozoal population in the rumen has a negative effect on the amino acid (AA) supply to ruminants and contribute to generate more methane but, nevertheless, protozoa must not be considered as parasites. They are useful for numerous reasons. They stabilise rumen pH when animal are fed diets rich in available starch and decrease the redox potential of rumen digesta. Because cellulolytic bacteria are very sensitive to these two parameters, protozoa indirectly stimulate the bacterial cellulolytic activity and supply their own activity to the rumen microbial ecosystem. They could also supply some peptides in the rumen medium which can stimulate the growth of the rumen microbiota, but this aspect has never been considered in the past. Their high contribution to ammonia production has bad consequences on the urinary nitrogen excretion but means also that less dietary soluble nitrogen is necessary when protozoa are present. Changes in the molar percentages of VFA and gases from rumen fermentations are not so large that they could alter significantly the use of energy by animals. The answer of animals to elimination of protozoa (defaunation) depends on the balance between energy and protein needs of animals and the supply of nutrients supplied through the diet. Defaunation is useful in case of diets short in protein nitrogen but not limited in energy supply for animals having high needs of proteins.

• Asian-Australasian Journal of Animal Sciences
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• 제28권2호
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• pp.215-222
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• 2015

The objective of this research was to evaluate the growth performance, the apparent ileal digestibility of nitrogen and energy, the retention of nutrients and the apparent metabolizable energy corrected to zero nitrogen retention (AMEn) in broiler chickens supplemented with increasing doses of a worm leachate (WL) as a source of humic substances (HS) in the drinking water. In Exp. 1, 140 male broilers were penned individually and assigned to four WL levels (0%, 10%, 20%, and 30%) mixed in the drinking water from 21 to 49 days of age. Water was offered in plastic bottles tied to the cage. In Exp. 2, 600 male broilers from 21 to 49 days of age housed in floor pens were assigned to three levels of WL (0%, 10%, and 20%) mixed in the drinking water. The WL was mixed with tap water in plastic containers connected by plastic tubing to bell drinkers. The results of both experiments were subjected to analysis of variance and polynomial contrasts. In Exp. 1, the daily water consumption was similar among treatments but the consumption of humic, fulvic, and total humic acids increased linearly (p<0.01) as the WL increased in the drinking water. The feed conversion (p<0.01) and the ileal digestibility of energy, the excretion of dry matter and energy, the retention of dry matter, ash and nitrogen and the AMEn showed quadratic responses (p<0.05) relative to the WL levels in drinking water. In Exp. 2, the increasing level of WL in the drinking water had quadratic effects on the final body weight, daily weight gain and feed conversion ratio (p<0.05). The addition of WL as a source of HS in the drinking water had beneficial effects on the growth performance, ileal digestibility of energy, the retention of nutrients as well on the AMEn in broiler chickens; the best results were observed when the WL was mixed at levels of 20% to 30% in the drinking water.