• 제목/요약/키워드: Nitrogen analysis

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The effect of nitrogen-fixing microorganisms on plant promotion in cabbage

  • Moon, Je-Hun;Jadamba, Chuluuntsetseg;Yoo, Soo-Cheul
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.190-190
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    • 2017
  • Chemical fertilizers have been used to increase crop production and contributed to escaping food shortages. However, excessive use of chemical fertilizers over a long period caused many problems such as environmental pollution and the hampered production potential of the land. Thus, it is necessary to develop eco-friendly bio-fertilizers that can replace the use of chemical fertilizers. Here, we tested the effect of some nitrogen-fixing microorganims on the plant growth promotion. Seventy free-living nitrogen fixing microorganisms were isolated from rhizosphere of crop cultivation fields, streamside soils and sludge in Ansung, Korea. Of them, three strains (NF2-4-1, Yeast; EMM409, Mesorhizobium; Gsoil662, Burkholderia) were selected to be most efficient in the capacity of N-fixing nitrogen based on colony forming cell assay in N-free media. To investigate the ability to promote plant growth, these strains were inoculated into the soil and cabbage were grown for 4 weeks in the grown chamber. Fresh weight, dry weight, and leaf area were measured from 4-week-old plants. Phenotypic analysis revealed that the growth of the plants inoculated with NF2-4-1 and EMM409 strains were significantly promoted compared to the mock-treated control plants, while Gsoil662-inoculated plants did not show statically significant promotion. These results indicate that these nitrogen-fixing microorganims can be used to develop plant growth promoting bio-fertilizers. Further analysis on nitrogen fixing level in soil by these strains will be tested.

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Long-term Seasonal and Interannual Variability of Epilimnetic Nutrients (N, P), Chlorophyll-a, and Suspended Solids at the Dam Site of Yongdam Reservoir and Empirical Models

  • An, Kwang-Guk
    • 생태와환경
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    • 제44권2호
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    • pp.214-225
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    • 2011
  • The objectives of the study were to evaluate seasonal patterns of epilimnetic water quality, and determine interannual eutrophication patterns at the dam site of Yong-dam Reservoir using long-term data during 2002~2009. Ionic dilutions, based on specific conductivity, occurred in the summer period in response to the intense monsoon rain and inflow, and suspended solid analysis indicated that the reservoir was clear except for the monsoon. Seasonality of nitrogen contents varied depending on the types of nitrogen and responded to ionic dilution; Ammonia-nitrogen ($NH_4$-N) peaked at dry season but nitrate-nitrogen ($NO_3$-N) peaked in the monsoon when the ionic dilution occurred. The maxima of $NO_3$-N seemed to be related with external summer N-loading from the watershed and active nitrogen fixation of bluregreens in the summer. $NO_3$-N was major determinant (>50%) of the total nitrogen pool and relative proportion of $NH_4$-N was minor. Long-term annual $NO_3$-N and TDN showed continuous increasing trends from 2004 to 2009, whereas TP and TDP showed decreasing trends along with chlorophyll-a (CHL) values. Empirical model analysis of log-transformed nutrients and N : P ratios on the CHL showed that the reservoir CHL had a stronger linear function with TP ($R^2$=0.89, p<0.001) than TN ($R^2$=0.35, p=0.120). Overall results suggest that eutrophication progress, based on TP and CHL, is slow down over the study period and this was mainly due to reduced phosphorns, which is considered as primary nutrient by the empirical model.

심랭식공기분리공정에서 질소증류탑의 엑서지 해석 (Exergy Analysis of Nitrogen Distillation Column in the Cryogenic Air Separation Process)

  • 용평순;이성철
    • 한국초전도ㆍ저온공학회논문지
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    • 제4권1호
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    • pp.145-150
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    • 2002
  • The distillation column is one of large energy consumable units in the cryogenic air separation process and the accurate energy analysis of this unit is necessary for choice of energy saving process. In this work, the energy method was adopted for energy analysis of a cryogenic nitrogen distillation column. In order to designing the energy saving distillation column, the exergy distribution of feed air, exergy efficiency and exergy loss for process condition was investigated and the optimal process condition to minimize the exergy loss was found. The result from this work can be used as a guideline for the choice of the process design conditions and efficiency improvement of cryogenic distillation column.

Optimization of main factors using response surface method for the enhanced production of hGM-CSF from transgenic Nicotiana tabacum cell suspension cultures

  • Lee, Ki-Yong;Lee, Sang-Yoon;Kim, Dong-Il
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
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    • pp.351-355
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    • 2003
  • Screening 실험을 통하여 결정된 주요인자인 sucrose, nitrogen, 배양온도로 인자의 최적수준을 결정하는 표면반응법중 하나인 Box-Behnken design을 수행하였다. 각 인자의 상관관계를 통하여 sucrose는 90 g/L, nitrogen은 41 mM, 온도는 $22^{\circ}C$가 최적수준으로 결정이 되었으며 확인실험을 통하여 대조구보다 세포생장이 증가하였을 뿐만 아니라 hGM-CSF의 생산도 약 2배 정도 증가되었음을 확인하였다. 이것은 고농도의 sucrose로 인해 배지내로의 hGM-CSF의 분비가 촉진되었기 때문이며 또한 저온으로 인해 분비된 hGM-CSF를 분해하는 protease 활성이 감소되었기 때문인 것으로 사료된다.

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77K 이하 극저온 상에서의 부분방전 특성 분석 (Analysis of Partial Discharge Characteristics at Cryogenic Temperature below 77K)

  • 이상화;김복렬;신우주;이방욱;구자윤
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2011년도 제42회 하계학술대회
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    • pp.1562-1563
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    • 2011
  • Partial discharge measurement is one of the effective diagnostic techniques to predict abnormal high voltage dielectric insulation conditions of the electric equipments. Recently partial discharge diagnostic techniques were also utilized to evaluate the cryogenic dielectric insulation of high temperature superconducting electric equipment in liquid nitrogen. Generally, liquid nitrogen at 77 K is used used as the cryogenic and dielectric media for many high temperature superconducting high voltage applications. When a quench in the superconductor occurs, bubbles are generated which can affect the dielectric properties of the liquid nitrogen. So in order to reduce the bubble formation, subcooled nitrogen was also employed for this purpose. In this work, investigation of partial discharge characteristics of subcooled liquid nitrogen were conducted in order to clarify the retardation of partial discharge initiation voltage according to the different subcooling temperature of liquid nitrogen. And also the relation of partial discharge phenomena and the activities of bubbles were analyzed. It was observed that PD inception voltages shows rather different characteristics according to the decrease of subcooling temperature and the activities of bubbles were strongly influenced by temperature of the subcooled liquid nitrogen.

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대기 입자상물질중 탄소(C)등의 열적특성에 관한 조사연구 (A Study on Thermal Characteristic Carbon and other Elements in Suspended Particulates)

  • 황경철
    • 한국환경보건학회지
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    • 제16권2호
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    • pp.31-39
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    • 1990
  • An analytical method for particulate carbon and other elements by using elemental analyzer was investigated. Carbon, hydrogen, and nitrogen was determined as CO$_{2}$, H$_{2}$, and N$_{2}$, respectively. Organic was determined after scparation from elemental carbon(Cae) by volatilization and thermal decomposition in a heated helium flow. With organic materials examined in this reprot, more than 90% of carbon was detected as above 600$^{\circ}$C. But it is considered that a few percents of some compounds were charred above 550$^{\circ}$C. A small amount of Cae was oxidized in the inert atmosphere above 850$^{\circ}$C, but the reason was not explained clearly. Based on the thermal chracteristics of Cao it was found that the optimum temperature of heating in the helium flow of an elemental analyzer for Cao analysis is 630$^{\circ}$C. Carbon in a sample after removing Cao was assumed as Cae and the gramatom ratio of hydrogen to carbon in the sample was 0.4 and less. Rescovery of nitrogen derived from some ammonium salts and nitrates was 100% by two-step measurement with elemental analyzer. By the analytical method investigated in this report, carbon and other elements in suspended particulates(S.P) collected at an urban area in Seoul were measured. There was a good correlation between total nitrogen in SP measured by elemental analyzer and nitrogen estimated form ammonium ions and nitrate ions in SP. The nitrogen from these ions accounts for 80% of the total nitrogen. It is further suggested that the residue(20%) of the total nitrogen is attributed to the other nitrogeneous compounds.

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천연 제올라이트를 이용한 고농도 질소 회수 (Recovery of nitrogen from high strength waste stream by using natural zeolite (Clinoptilolite))

  • 최오경;이관형;;이재우
    • 상하수도학회지
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    • 제30권1호
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    • pp.105-111
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    • 2016
  • This paper presents the applicability of natural zeolite (Clinoptilolite) for recovery of ammonium nitrogen from high-strength wastewater stream. Isotherm experiments showed the ammonium exchange Clinoptilolite followed Freundlich isotherm and its maximum exchange capacity was $18.13mg\;NH_4{^+}-N/g$ zeolite. The X-ray photoelectron spectroscopy (XPS) analysis indicated that a significant amount of nitrogen was adsorbed to the Clinoptilolite. Optimal flowrate for recovery of high concentration ammonium nitrogen was determined at 16 BV/d (=19.2 L/min) throughout the lab-scale column studies operated under various flowrate conditions. This study also provided a method to determine the recovery rate of final product of nitrogen fertilizer based on the model application to the lab-scale continuous data.

Effects of Fertilizer on Growth, Carbon and Nitrogen Responses of Foliage in a Red Pine Stand

  • Kim, Choonsig;Ju, Nam-Gyu;Lee, Hye-Yeon;Lee, Kwang-Soo
    • 한국토양비료학회지
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    • 제46권1호
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    • pp.1-7
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    • 2013
  • This study was to examine growth, carbon and nitrogen responses in foliage following forest fertilization in a red pine stand. Two types of fertilizer (N:P:K=113:150:37 kg $ha^{-1}$; P:K=150:37 kg $ha^{-1}$) were applied on late April 2011. Growth, carbon and nitrogen responses of foliage were monitored 3 times (July, September, November) after fertilization. Morphological growth responses (dry mass, leaf area, specific leaf area) with foliage age were not significantly (P > 0.05) affected by fertilizer application, while needle dry mass and leaf area of July were significantly lower in current-year-old than in one-year-old or two-year-old needles of September or November. Carbon concentration and content in foliage was little affected by fertilizer application compared with sampling month or needle age, while the NPK fertilizer produced high nitrogen concentration and content of foliage. The results indicate that nitrogen concentration and content in foliage may serve as an indicator of the nitrogen status by fertilization in a red pine stand.

Influence of Thermodynamic Properties upon Transcritical Nitrogen Injection

  • Tani, Hiroumi;Teramoto, Susumu;Nagashima, Toshio
    • 한국추진공학회:학술대회논문집
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    • 한국추진공학회 2008년 영문 학술대회
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    • pp.320-329
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    • 2008
  • The influence of thermodynamic transition associated with transcritical nitrogen injection upon the flow structure was investigated to explore numerical simulation of the injectant dynamics of oxygen/hydrogen coaxial jet in liquid rocket engines. Single and coaxial nitrogen jets were treated by comparing the transcritical and perfect-gaseous conditions, wherein the numerical model was accommodative to the real-fluid thermodynamics and transport properties at supercritical pressures. The model was in the first place validated by comparing the results of transcritical nitrogen injection between calculations and available experiments. For a single jet under the transcritical condition, the nitrogen kept a relatively high density up to its pseudo-critical temperature inside the mixing layer, since it remains less expanding until heated up to its pseudo-critical temperature. Numerical analysis revealed that cryogenic jets exhibit strong dependence of specific enthalpy profile upon the associated density profile that are both dominated by turbulent thermal diffusion. In the numerical model, therefore, exact evaluation of turbulent heat fluxes becomes very important for simulating turbulent cryogenic jets under supercritical pressures. Concerning the coaxial jets due to transcritical/gaseous nitrogen injections, the density profile inside the mixing layer was again affected by the thermodynamic transition of nitrogen. However, hydrodynamic instability modes of the inner jet did not show significant differences by this thermodynamic transition, so that further study is needed for the mixing process downstream of the near injection position.

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Effects of Different Nitrogen Application Levels on Yield and Protein Content of Rice Varieties in Korea

  • Jinseok Lee;Jong-Seo Choi;Shingu Kang;Dae-Woo Lee;Woonho Yang;Seuk-Ki Lee;Su-Hyeon Sin;Min-Tae Kim
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.123-123
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    • 2022
  • In order to evaluate the effect of nitrogen application levels on yield and protein content of rice varieties, a field experiment was conducted at National Institute of Crop Science of Korea in 2020. Five levels(0, 3, 5, 7, and 9 kg·10a-1) of nitrogen fertilizer were treated to six Korean rice varieties. The nitrogen uptake amount, soil nitrogen content before and after rice cultivation, milled rice yield, and protein content in head rice were analyzed. As the treatment of nitrogen fertilizer increased, the nitrogen uptake amount of plants increased significantly. However, changes in nitrogen content in the soil before and after rice cultivation were different for each cultivar. The amount of nitrogen change in the soil decreased as the amount of nitrogen application increased in the three cultivars of Haepum, Gopum, and Odae, and the other three cultivars showed the opposite trend. As a result of correlation analysis of nitrogen application amount, nitrogen uptake amount, milled rice yield, and protein content of head rice, the five varieties except for Haepum showed a high correlation between these factors. The amount of nitrogen application and nitrogen uptake of plant showed a positive correlation about the milled rice yield and protein content of head rice. In particular, the protein content in head rice appeared to be more affected by nitrogen uptake amount than nitrogen application amount. As a result of this study, the yield and protein content of rice had positive correlations with the level of nitrogen fertilizer, and had a high correlation with the nitrogen absorption of plants.

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