• Environmental Engineering Research
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• v.19 no.1
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• pp.91-99
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• 2014

Ordinary heterotrophic organism (OHO) active biomass plays key roles in biological wastewater treatment processes. However, due to the lack of measurement techniques, the OHO active biomass exists hypothetically within the design and simulation of biological wastewater treatment processes. This research was purposed to develop a quick and easy quantifying technique for the OHO active biomass applying a modified batch aerobic growth test. Two nitrification-denitrification activated sludge systems, with 10- and 20-day sludge ages, were operated to provide well-cultured mixed liquor to the batch tests. A steady state design model was firstly applied to quantify the "theoretical" OHO active biomass concentration of the two parent systems. The mixed liquor from the parent systems was then inoculated to a batch growth test and a batch digestion test to estimate the "measured" OHO active biomass concentration in the mixed liquor. The measured OHO active biomass concentrations with the batch growth test and the batch digestion test were compared to the theoretical concentrations of the parent system. The measured concentrations with the batch growth test were generally smaller than the theoretical concentrations. However, the measured concentrations with the batch aerobic digestion tests showed a good correlation to the theoretical concentrations. Thus, a different microbial growth condition (i.e., a higher food/biomass ratio) in the batch growth test, compared to the parent system or the batch digestion test, was found to cause underestimation of the OHO active biomass concentrations.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.450-455
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• 2005

To treat wastewater efficiently by a one-step process of nitrogen removal, a new strain of $N_2-producing$ bacteria from $NH{_4}{^+}$ under an aerobic condition was isolated and identified. By 16S-rDNA analysis, the isolate was identified as Enterobacter asburiae with 96% similarity. The isolate shows that the capacity of $N_2$ production under an oxic condition was approximately three times higher than that under an anoxic condition. The optimal conditions (pH, temperature and C/N ratio) of the immobilized isolate for $N_2$ production were found to be 7.0, $30^{\circ}C$ and 5, respectively. Under all the optimum reaction conditions, the removal efficiency of $COD_{Cr}$ and TN reached 56.1 and 60.9%, respectively. The removal rates of $COD_{Cr}$ and TN were highest for the first 2.5 hrs (with the removal $COD_{Cr}$ ratios of 32.1), and afterwards the rates decreased as reaction proceeded. For application of the immobilized isolate to a practical process of ammonium removal, a continuous bioreactor system exhibited a satisfactory performance at HRT of 12.1 hr, in which the effluent concentrations of $NH{_4}{^+}-N$ was measured to be 15.4 mg/L with its removal efficiency of 56.0%. The maximum removal rate of $NH{_4}{^+}-N$ reached 1.6 mg $NH{_4}{^+}-N/L/hr$ at HRT of 12.1 hr (with N loading rate of 0.08 $Kg-N/m^3-carrier/d)$. As a result, the application of the immobilized isolate appears a viable alternative to the nitrification-denitrification processes.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.6
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• pp.791-799
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• 2005

The phased isolation intra-clarifier ditch system used in this study is a simplified novel process enhancing simultaneous removal of biological nitrogen and phosphorus in municipal wastewater in terms of elimination of additional pre-anaerobic reactor, external clarifier, recycle of sludge, and nitrified effluent recirculation by employing intrachannel clarifier. Laboratory-scale phased isolation ditch system was used to assess the treatability on municipal wastewater. When the system was operated at the HRTs of 6~12hours, SRTs of 9~31days, and cycle times of 2~8hours, the system showed removals of BOD, TN, and TP as high as 88~97%, 70~84%, and 65~90%, respectively. The rainfall in Korea is generally concentrated in summer because of site-specific characteristics. Especially, the wet season has set in on June to August. In combined sewers, seasonal variations are primarily a function of the amount of stormwater that enters the system. In order to investigate the effect of hydraulic shock loading on system performance, the laboratory-scale system was operated at an HRT of 6hours (two times of influent flowrate) during two cycles (8hours). The system performance slightly decreased by increasing of influent flowrate and decreasing of system HRT. Nitrification efficiency and TN removal were slightly decreased by increasing of influent flowrate (decreasing of system HRT), whereas, the denitrification was not affected by hydraulic shock loading. However, the higher system performance could be achieved again after four cycles. Thus, the phased isolation technology for enhanced biological nutrient removal in medium- and small-scale wastewater treatment plants suffering fluctuation of influent quality and flowrate.

• Ocean and Polar Research
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• v.25 no.4
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• pp.493-501
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• 2003

Performance of a laboratory scale closed seawater recirculating aquaculture system was evaluated. Twenty-kg of korean rockfish (130 fish) with an average body weight of 153.8g was stocked. Over a 107-day culture period, fish reached final density of $51.7kg/m^3$ (initial density, $33.3kg/m^3$) on the basis of the culture tank volume. On a daily basis, added water amounted to 3.4% of the total water volume in the system. Total ammonia nitrogen (TAN) concentrations were below 1mg/l and nitrite nitrogen $(NO_2-N)$ concentrations were within the range of 1-3mg/l on most sampling days. TAN was removed from bead and sand filters and it was removed or produced in the sedimentation basin. Basically, $NO_2-N$ was removed in the bead and sand filters, while it was either removed or produced in the sedimentation basin. Nitrate nitrogen $(NO_3-N)$ was produced in the bead filters and removed from the sand filter and sedimentation basin. The foam fractionator performed well in the recirculating system. The maximal daily removal values for total suspended solids (755) and protein were 10.9g and 1.4g, respectively. Whole water quality parameters were within the levels commonly recommended for fish culture on most of the sampling days. However, further studies are needed to evaluate the commercial feasibility of this system because of the smallscale system used in present experiment. At least, the present study still provides some basic information for further studies of this kind of system.

• Journal of the Korea Organic Resources Recycling Association
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• v.13 no.4
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• pp.128-135
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• 2005

This study was done to improve the effectiveness of nitrification and denitrification using the aeration-anoxic combination method using CFSTR(continuous-flow stirred-tank reactor) attached with an anoxic reactor filled with a media. In order to calculate the concentration of nitric acid within the aeration tank proportional to the anoxic rate within the reactor, supernatant within the inflow and precipitation tanks were influxed into the anoxic reactor. The rate of nitrogen removal was calculated using the concentration of inflow and flow of returned supernatant. From the results of this experiment, the carbon source needed in the anoxic reactor came from the inflow so that anoxification was achieved completely using the inflow source without the introduction of an external carbon source. However, as the ratio of nitric acid becomes large in inflow and nitric acid flow, the carbon source within the input source decreases so that the concentration of carbon source is important.

• Journal of Soil and Groundwater Environment
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• v.10 no.3
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• pp.9-15
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• 2005

Soil aquifer treatment is a water reuse technology that secondary or tertiary treated wastewater is infiltrated into the aquifer in which physical and biochemical reactions occur. Major consideration in SAT is the removal and transport of DOC and nitrogen species. In this study, reaction mechanism in SAT was examined considering nitrification, denitrification and organic oxidation. In addition, SAT modeling system was developed as the reaction mechanism was applied to groundwater flow and transport model. In verification of the reaction module by 1-dimensional unsaturated soil column test, the experimental data of all of the species, ammonium, nitrate, DOC and DO, were well matched with the simulation results. In sensitivity analysis, ammonium partition coefficient, dissolved oxygen inhibition constant and biomass decay rate affect ammonium, DOC and DO concentration of effluent, respectively.

• Journal of Korean Society on Water Environment
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• v.20 no.6
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• pp.563-570
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• 2004

Phased isolation intrachannel clarifier ditch process developed in this study is an enhanced biological nutrient removal process employing two ditches with intrachannel clarifiers. Bench-scale phased isolation ditch process was used to evaluate the system performance on municipal wastewater and detailed assessment of internal behavior in a ditch and each reactions. When the system was operated at the HRTs of 6~12hours, SRTs of 9~31 days, and cycle times of 4hours, the system showed removals of BOD, TN, and TP as high as 88~97%, 73~78%, and 65~90%, respectively. The internal behavior were well matched on each reactions such as nitrification, denitrification, and phosphorus release and uptake. As the SRT became longer, TN removal increased gradually, whereas TP removal decreased contrarily. However, the system was capable of producing an effluent TP concentration 1mg/L or less even at longer SRTs except the case of solids discharge by malfunction of intra-clarifier occurred by its geometrical limit. The system performance slightly decreased by hydraulic shock loading(increasing of influent flowrate and decreasing of system HRT). However, the higher system performance could be achieved again after four cycles. Thus, the system reliability could be successfully achieved short-term hydraulic shock loading that occurred in medium- and small-sized wastewater treatment plants suffering fluctuation of influent quality and flowrate during wet season.

• Journal of Korean Society of Water and Wastewater
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• v.28 no.6
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• pp.691-698
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• 2014

This study investigated the effect of a co-culture of Scenedesmus dimorphus and nitrifiers using artificial wastewater on the removal of ammonium, nitrate and phosphate in the advanced treatment. To test the synergistic effect of the co-culture, we compared the co-culture treatment with the cultures using S. dimorphus-only and nitrifiers-only treatment as controls. After 6 days of incubation, nitrate was removed only in the co-culture treatment and total amount of N removal was 1.3 times and 1.6 times higher in the co-culture treatment compared to those in the S. dimorphus- and nitrifiers-only treatments, respectively. In case of total amount of P, co-culture treatment removed 1.2 times and 12 times more P than the S. dimorphus -and nitrifiers-only conditions, respectively. This indicates that the co-culture improved removal rates for ammonium, nitrate, and phosphate. This further implies that there was no need for denitrification of nitrate and luxury uptake of P processes because nitrate and phosphate can be removed from the uptake by S. dimorphus. In addition, co-culture condition maintained high DO above 7 mg/L without artificial aeration, which is enough for nitrification, implying that co-culture has a potential to decrease or remove aeration cost in the wastewater treatment plants.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.1
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• pp.37-44
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• 2005

Nitrate($NO_3-N$) and total nitrogen(TN) removal by a reed wetland with open water(Wetland 1) was compared with that of a reed wetland without open water(Wetland 2) from March to October 2002. The two wetlands were 25mL by 6mW. An open water area, 3mL by 6mW was designed at the middle of Wetland 1. Reeds(Phragmites australis) were transplanted into the wetlands in June 2000. Water of Sinyang Stream flowing into the Kohung Estuarine Lake located in the southern part of Korea was pumped into a primary treatment pond, whose effluent was discharged into the secondary pond. Effluent from the secondary pond was funneled into the wetlands. Inflow into the wetlands averaged about 20.0$m^3$/day and their hydraulic retention time was approximately 1.5 days. Average $NO_3-N$ removal by Wetland 1 was 117.61mg/$m^2{\cdot}day$ and that by Wetland 2 was 106.39mg/$m^2{\cdot}day$. $NO_3-N$ removal efficiency of Wetland 1 and 2 was 37% and 34%, respectively. TN removal by Wetlands 1 and 2 averaged 226.80 and 214.54mg/$m^2{\cdot}day$, respectively. TN abatement efficiency of Wetland 1 was 43% and that of Wetland 2 was 40%. $NO_3-N$ removal efficiency of Wetland 1 was significantly higher(p=0.038) than Wetland 2. TN removal efficiency of Wetland 1 was also significantly higher(p=0.044) than Wetland 2. The wetland with open water was more efficient for removal of $NO_3-N$ and TN than one without.

• Journal of Korean Society on Water Environment
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• v.22 no.1
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• pp.74-82
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• 2006

In this study, development of M2-Dephanox and M3-Dephanox process has been tried to enhance the nitrogen removal of M-Dephanox process on the basis of previous study about M-Dephanox. The results showed that T-N removal efficiency of M3-Dephanox process was 8.9% or 11.3% higher than M-Dephanox or M2-Dephanox processes, respectively. This result is due to the lower $NO_3{^-}-N$ concentration in the effluent of M3-Dephanox than of M-Dephanox and M2-Dephanox processes. This results were recurrenced by PASS simulator. As result of simulation by PASS program, effluent $NO_3{^-}-N$ concentration of M3-Dephanox process was 1.4 mg/L and 3.6 mg/L lower than M-Dephanox and M2-Dephanox processes. In the study about optimization of M3-Dephanox processes by PASS program, SRT greatly affected T-N removal of M3-Dephanox process, whereas, the recycle rate and recirculation rate did little affect T-N removal efficiency of M3-Dephanox. In the study about optimization of reactors following the nitrification reactor of M3-Dephanox process, it was shown that the best optimum volume ratio of denitrification reactor, intermittently aerated reactor and anoxic reactor for the T-N removal were 29.1(%) : 32.7(%) : 38.2(%). T-N removal efficiency at this volume ratio was similar to T-N removal efficiency at the volume ratio of 36.3(%) : 36.3(%) : 27.4(%) designed for the lab-scale M3-Dephanox.