• Asian Journal of Turfgrass Science
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• v.5 no.1
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• pp.41-45
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• 1991

서양잔디중 난지형 잔디에 속하는 세인트 어거스틴그래스에서 암모늄태 질소와 질산태 질소질 비료의 시비가 브라운팻치 병 발생에 미치는 영향을 알아보고자 본 실험이 수행되었다. 포장에서 재배되고 있는 세인트 어거스틴그래스를 작경 10cm의 플러그를 굴취하여 플라스틱 포트에 이식한 후 뿌리의 활착을 위하여 온실내에서 한달간 재배하였고, 50kg N/ha의 성분비율에 맞추어서 균의 접종 1일전에 시비를 하였다. 잔디포장에서 분리한 Rhizoctonia solani균을 agar위에서 배양시킨 후 균이 배양되어 있는 agar block 을 떼어서 태운 perennial ryegrass에 접종시켰다. 감염된 perennial ryegrass를 다시금 세인트 어거스틴그래스위에다 치상시켜서 잔디로의 접종을 꾀하였다. 그 결과 암모늄태 질소비료나 질산태 질소비료 그리고 무시비상태의 잔디에서의 병발생간에는 유의차가 인정되지 않았으나 질산화 억제제를 처리하여 암모늄상태의 질소 성분이 질산태로 전환되는 것을 억제시킨 구에서는 타처리구에 비하여 6~9배의 발생율을 보였다. 따라서 잔디의 시비와 병발생을 연관시켜 볼 때 암모늄태 질소비료 와 질산태 질소비료 공히 큰 차이는 없으나, 다만 질산태로 변한 후의 질소양분의 토양용탈로 인한 소모를 억제시키기 위하여 사용되는 질산화 억제제는 상당량의 암모늄태 질소성분을 상당 기간동안 유지시키기 때문에 브라운팻치 병발생에 영향을 주므로 그 사용시기에 있어서 신중을 기하여야 하겠다.

• Journal of Korean Society on Water Environment
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• v.23 no.2
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• pp.188-192
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• 2007

The biochemical oxygen demand (BOD) test is widely used to determine the pollution strength of water, to evaluate the performance of wastewater treatment plants and to judge compliance with discharge permits. However, nitrification is a cause of significant errors in measuring BOD, particularly when a large population of nitrifying organisms is existing in water such as effluents from biological treatment plants. In order to investigate the amount of nitrogenous oxygen demand (NOD), BOD with and without inhibitor was measured as samples in the biological treatment plants. About 81% of effluent BOD from the biological treatment plant used in this experiment was comprised of NOD. In the case of influents, the NOD accounted for about 9% of BOD. The inhibited 5-day BOD (Carbonaceous BOD) test must be considered in evaluating the performance of wastewater treatment plant and judging compliance with discharge permit limitations.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.3
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• pp.265-273
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• 1984

To investigate nitrogen supplying effect of some slowrelease N-fertilizers on barley in upland soil sulfurcoated urea(SCU), ureaform and melamine (1,3,5-Triazine-2,4,6-triamine) were treated and compared to urea. In addition, thiourea as a nitrification inhibitor was also tested. Effects of variable soil condition such as moisture content, pH and temperature on nitrogen supplying ability of the fertilizers and on growth of barley were studied through incubation test and pot culture and the obtained results were summarized as follows: The releasing rate of ammonia from urea, SCU, ureaform and melamine were resulted as 27-59%, 25-39%, 9-34% and 0.7-4.3% at maximum conversion rate, respectively. Nitrification rate of the tested fertilizers was higher at pH 6.54 markedly than at pH 4.73. Addition of thourea depressed the formation of $NO_3$ during four weeks of incubation period. Mixed application of ureaform with small amount of urea contributed to nitrogen supply till latter growth stage of barely Basal application of melamine showed lowest nitrogen supplying ability and injurious response on barley growth.

• Journal of Korean Society of Water and Wastewater
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• v.21 no.6
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• pp.727-735
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• 2007

The use of water by cities is increasing owing to industrialization, the concentration of population, and the enhancement of the standard of living. Accordingly, the amount of waste water is also increasing, and the degree of pollution of the water system is rising. In order to solve this problem, it is necessary to remove organisms and suspended particles as well as the products of eutrophication such as nitrates and phosphates. This study developed a high-end treatment engineering solution with maximum efficiency and lower costs by researching and developing a advanced treatment engineering solution with the use of Biosorption. As a result, the study conducted a test with a $50m^3/day$ Pilot Scale Plant by developing treatment engineering so that only the secondary treatment satisfies the standard of water quality and which provided optimal treatment efficiency along with convenient maintenance and management. The removal of organisms, which has to be pursued first for realizing nitrification during the test period, was made in such a way that there would be no oxidation by microorganisms in the reactor while preparing oxygen as an inhibitor for the growth of microorganism in the course of moving toward the primary settling pond. The study introduced microorganisms in the endogeneous respiration stage to perform adhesion, absorption, and filtering by bringing them into contact with the inflowing water with the use of a sludge returning from the secondary settling pond. Also a test was conducted to determine how effective the microorganisms are as an inner source of carbon. The HRT(Hydraulic Retention Time) in the nitrification tank (aerobic tank) could be reduced to two hours or below, and the stable treatment efficiency of the process using the organisms absorbed in the NAR reactor as a source of carbon could be proven. Also, given that the anaerobic condition of the pre-treatment tank becomes basic in the area of phosphate discharge, it was found that there was excellent efficiency for the removal of phosphate when the pre-treatment tank induced the discharge of phosphate and the polishing reactor induced the uptake of phosphate. The removal efficiency was shown to be about 94.4% for $BOD_5$. 90.7% for $COD_{Cr}$ 84.3% for $COD_{Mn}$, 96.0% for SS, 77.3% for TN, and 96.0% for TP.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.412-420
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• 2005

In order to investigate factors affecting the denitrification in the F-STEP PROCESS using a loess ball as support media and Pseudomonas DWC 17-8, calcining temperature, loess ball size, pH, nitrate concentration, working temperature, and inhibitor were studied in batch mode using synthetic sludge. A 5- 10 mm of loess ball (960$^{circ}$ of calcining temperature) was the most suitable for denitrification. When the initial pH was increased from 3.0 to 7.0, the removal efficiency of nitrate was increased. Specifically, at initial pH of 7.0, the maximum removal efficiency of nitrate was 5.0 mg/min. When the initial concentration of nitrate was increased from 100 to 400 mg/l, the removal efficiency of nitrate was proportional to the concentration of nitrate. The maximum removal efficiency of nitrate was 5.72 mg/min at 400 mg/l of initial concentration. When the operating temperature was increased from 10 to 30$^{circ}$, the removal efficiency of nitrate was increased from 0.76 to 6.15 mg/min, and at above 40$^{circ}$ of operating temperature, it was decreased from 4.0 to 2.0 mg/min. Among the various inhibitors, higher than 10$^{-1}$ M of sodium azide abolished this reaction completely. When the KCN concentration was above 10$^{-1}$ M, the reaction was inhibited completely. In the case of 2,4-dinitrophenol and sodium sulphide, it was inhibited at above 10$^{-2}$ M completely. For testing the various flow orders of the F-STEP PROCESS for effective denitrification using practical wastewater, continuous experiments under the optimum conditions were carried out for 60 days. Among the various processes, the PROCESS A gave the highest efficiencies of denitrification, nitrification, and total nitrogen (TN) removal with 86.5, 89.5, and $90\%$, respectively. For scale-up in the PROCESS A, real farm wastewater was used and pilot tests carried out for 90 days. The denitrification efficiency was $97.5\%$, which was increased by $12.7\%$. The efficiencies of TN removal and nitrification were 96.6 and $70.0\%$, respectively. The removal efficiency of chemical oxygen demand (COD) was $63.7\%$, which was increased by $20\%$.

• Journal of Applied Biological Chemistry
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• v.44 no.3
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• pp.135-139
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• 2001

To observe the changes in isotopic composition (${\delta}^{15}N$) of $NO_3{^-}$ during denitrification, an incubation experiment using soil treated with nitrification inhibitor (2-chloro-6-trichloromethyl-pyridine) under water-saturated condition was conducted for 153 h. The $NO_3-N$ concentration decreased from 73.3 to $20.6mg\;kg^{-1}$ during the incubation period, with denitrification rate constant of $0.00905h^{-1}$, and ${\delta}^{15}N$ values of $NO_3-N$ increased from +0.9 to +25.5‰ with decreasing the $NO_3-N$ concentration. The increase in the ${\delta}^{15}N$ values of $NO_3-N$ is due to kinetic isotope fractionation, which always results in $^{15}N$ enrichment of the substrate. The isotopic fractionation factor calculated in this study was 1.0196, an indication that 1.96% more $^{14}NO_3{^-}$ reacted at a given time interval than a comparable number of $^{15}NO_3{^-}$. The ${\delta}^{15}N$ values measured through the incubation study showed a good agreement with the results calculated from the Fochts isotope fractionation model. Our results suggest that when the ${\delta}^{15}N$ of $NO_3{^-}$ is used for tracing the fate of N, the kinetic isotope fractionation associated with denitrification must be taken into consideration.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.11
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• pp.1969-1976
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• 2000

It is a time consuming work to identify the inhibition of unknown chemicals or industrial wastewater. Thus it is needed to establish a fast assay tool for finding a toxicant source. Biomass activity and ammonia inhibition were measured by DHAINT method. Ammonia inhibition tests were comprised of total ammonia inhibition and free ammonia inhibition. Those inhibitions were carried out by nitrifier and heterotroph each other with nitrifier inhibitor. The ammonia inhibition was proportional to an amount of total ammonia and pH increase. It meaned that a free ammonia played a key role for ammonia inhibition. however both total ammonia and free ammonia should be considered for an accurate assay of the ammonia inhibition. Nitrifier was more sensitive than heterotroph when the ammonia concentration above 3.000mg/L.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.1
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• pp.43-48
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• 1993

A laboratory study was cunducted to obtain fundamental informations on environmental-conservative treatment technique of soiled animalwaste. The release rate of inorganic nitrogen which mineralized from solid pigwaste and the effect of nitrification inhibitor(Dicyandiamide, DCD) on nitrate concentration of fresh or fermented pigwaste were weekly checked for ten weeks under incubation condition. Average pH of soild pigwaste was maintained over 8 unit during all incubation periods with no difference by nitrification inhibitor addition and the pHs of fresh- or fermented pigwaste without DCD were elevated by passing time 0.04, 0.058 pH unit/week (P<0.05) during incubation. While $NH_4-N$ concentration of fermented pigwaste until two week was nearly the same as it before incubation, $NH_4-N$ concentration of fresh pigwaste was remarkably increased upto 3,732 ppm for 1 week after incubation and the portion of increased $NH_4-N$ concentration, 2,473 ppm was 20.1% of indigenous organic nitrogen of fresh pigwaste. By the passing incubation time, $NH_4-N$ concentration began to lower linearly at not only fresh- but fermented pigwaste after 1 or 2 week, respectively and the $NH_4-N$ concentration loss rate at fresh pigwaste was 61.8 ppm/week with DCD addition and 72.3ppm/week with no DCD. There was positive relationship between $NO_3-N$ concentrations of fresh pigwaste by the addition of DCD or not and $NO_3-N$ concentraion was significantly lowered with DCD treatment($r=0.79^{**}$).

• Korean Journal of Environmental Agriculture
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• v.37 no.4
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• pp.235-242
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• 2018

BACKGROUND: About 81% of nitrous oxide ($N_2O$) emissions from agricultural land to the atmosphere is due to nitrogen (N) fertilizer application. Mitigation of $N_2O$ emissions can be more effective in controlling biochemical processes such as nitrification and denitrification in the soil rather than decreasing fertilizer application. The use of urease inhibitors is an effective way to improve N fertilizer efficiency and reduce $N_2O$ emissions. Several compounds act as urease inhibitors, but N-(n-butyl) thiophosphoric triamide (NBPT) has been used worldwide. METHODS AND RESULTS: Hot pepper and chinese cabbage were cultivated in five treatments: standard fertilizer of nitrogen-phosphorus-potassium(N-P-K, $N-P_2O_5-K_2O$: 22.5-11.2-14.9 kg/ha for hot pepper and $N-P_2O_5-K_2O$: 32.0-7.8-19.8 kg/ha for chinese cabbage), no fertilizer, and NBPT-treated fertilizer of 0.5, 1.0, and 2.0 times of nitrogen basal application rate of the standard fertilizer, respectively in Gyeonggi-do Hwaseong-si for 2 years(2015-2016). According to application of NBPT-treated fertilizer in hot pepper and chinese cabbage, $N_2O$ emission decreased by 19-20% compared to that of the standard fertilizer plot. CONCLUSION: NBPT-treated fertilizer proved that $N_2O$ emissions decreased statistically significant in the same growth conditions as the standard fertilization in the hot pepper and chinese cabbage cultivated fields. It means that NBPT-treated fertilizer can be applied for N fertilizer efficiency and $N_2O$ emissions reduction.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.3
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• pp.119-126
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• 2003

Nitrate-N concentrations and the corresponding ${\delta}^{15}N$ values were determined with water samples collected periodically from artesian wells (3 and 6 m deep), underdrainage and gushout waters in a Welsh onion cultivated area in the Kushibiki Fan, Saitama Prefecture, Japan. Average $NO_3-N$ concentrations in waters from 3 and 6 m wells were 25.7 and $2.8mg\;L^{-1}$, whereas ${\delta}^{15}N$ values were 3.6 and 4.7‰, respectively. The $NO_3-N$ concentration and ${\delta}^{15}N$ value of the underdrainge water were $35.5mg\;L^{-1}$ and 6.6‰, reflecting rapid input of chemical fertilizers and farmyard manure. The mean values of $NO_3-N$ concentration and ${\delta}^{15}N$ in the gushout water flown out of the edge of Kushibiki Fan were $19.4mg\;L^{-1}$ and 7.9‰, respectively. As a results the ${\delta}^{15}N$ values of the gushout water were higher than those of the artesian wells and underdrinage water. The ${\delta}^{15}N$ values of total-N and $NO_3-N$ of the soils were 6.1 and 5.10‰, respectively, while those for nitrification-inhibitor containing fertilizer and slow-release fertilizers were -6.1 and -2.2‰, respectively.