• Journal of Plant Biology
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• 제36권1호
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• pp.19-27
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• 1993

Effects of polyamines on DNA synthesis were studied in synchronized culture of Nicotiana tabacum L. When DFMO and DFMA, inhibitors of ornithine decarboxylase and arginine decarboxylase, respectively were initially applied to the cells, the polyamine contents were rapidly dropped and [methyl-3H] thymidine incorporation into DNA was markedly reduced during the early stage of culture period. Inhibition of DNA synthesis, however, was partially reversed when these inhibitors were applied simultaneously with putrescine. In addition, exogenous administration of putrescine also increased the DNA synthesis during the all over the culture period. In vitro activity of DNA polymerase from Nicotiana tabacum L. was promoted by increasing concentrations of polyamines in the reaction mixture. Maximal activity was shown at 5 mM putrscine, 0.5 mM spermidine and spermine, respectively. Lack of Mg2+ ion in the reaction buffer resulted in an inhibition of the enzyme activity by about 30%. The inhibition could not be completely reversed by application of polyamines at optimal concentrations. These results suggest that polyamines promote the DNA synthesis in vivo and in vitro by stabilizing the DNA-helix upon binding to negatively charged groups on DNA or increasing the activity of DNA polymerase in Nicotiana tabacum L.

• Journal of Plant Biology
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• 제30권1호
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• pp.1-9
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• 1987

Leaf mesophyll protoplasts of Nicotiana tabacum (nitrate reductase deficient mutant) were fused with cell suspension protoplasts of albino Petunia inflata in an electric field. Hybrid cell colonies were selected for nitrate reductase proficiency and chlorophyll synthesis. Five hybrid plant lines, regenerated from the selected calli lines, were analysed by electrophoresis, number of chromosomes and morphological characters. Somtic hybrid plants showed both parent patterns in the isozymesof isoleucine aminopeptidase and esterase. The hybrids had the expected chromosome number of 62 and exhibited an intermediate floral morphology when compared with the parents, but plant height and leaf arrangement were similar to N. tabacum.

• 한국작물학회지
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• 제11권
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• pp.11-80
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• 1972

• 한국연초학회지
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• 제16권2호
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• pp.102-107
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• 1994

This study was conducted to transfer the potato virus Y-necrosis strain resistance from Nicotiana africana to Nicotiana tabacum (cv. NC82) N. africana was crossed with NC82, GErmination of the cotyledon stage, at which time most of the seedling died. However, surviving seedlings continued to grow normally. Chromosomes of the these interspecific self-sterile F1 hybrids were doubled by tissue culture. Amphidiploid of F1 hybrid was self-fertile. Starting with amphidipliod, a wystematic backcross (BC) program was set up with NC82 as recurrent parent. In the BC5C2 generation, the resistant plant was selected. This resistant line, KF8833-1, had 48 chromosome and secreting glandular trichomes. It flowered 2days later than NC82, and stalk height, leaves per plant, leaf length and leaf width were similar to those of NC82.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 추계 학술대회지
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• pp.42-43
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• 1990

• Natural Product Sciences
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• 제7권1호
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• pp.17-20
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• 2001

The activity of the lipase and phospholipase extracted from the seed meal of Nicotiana tabacum L. was studied with the help of Spectrophotometer at different pH, Temperatures and Solvents. Both lipase and phospholipase shown optimum activity at pH 6 and $45^{\circ}C$. Their activities also maximize when n-heptane was used as solvent media.

• Natural Product Sciences
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• 제7권4호
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• pp.110-113
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• 2001

The lipid classes constituents; hydrocarbons, wax esters, sterol esters, triacylglycerols, free fatty acids, 1,3-diacylglycerols, 1,2-diacylglycerols, free sterols, 2-monoacylglycerols, 1-monoacylglycerols, phosphatidylethanolamines, phosphatidylcholines, lysophosphatidylethanolamines and phosphatidylinositols of Nicotiana tabacum L. seeds oil were investigated by thin layer and gas chromatography. Palmitic, oleic and linoleic acids were the major components in all lipid classes studied.

• Journal of Plant Biology
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• 제30권4호
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• pp.287-298
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• 1987

The regenerative capacities of protoplasts isolated from potato (Solamum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.317-320
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• 2000

유전자 재조합된 Nicotiana tabacum을 이용하여 동결 보존 후의 세포 생장을 관찰하였다. 형질 전환된 Nicotiana tabacum의 동결 보존에서 회복배지에 계면 활성제와 산소 전달 물질을 첨가한 경우 첨가하지 않은 경우에 비해 세포 생장을 증대 시켰고 산소 전달 물질 보다 계면활성제의 영향이 크다는 결과를 관찰하였다.

• 한국연초학회지
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• 제1권1호
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• pp.9-13
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• 1979

담배品種 ( Nicotiana tabacum L.) "SC 72"와 "Hicks" 를 文配하여 그$F_1$ 잡종식물의 葯으로부터 誘起한 반수체배가계통들의 특성을 檢定하였던 바, 交配親의 平均値에 비하여 生育特性은 현저히 저하하고, Total Alkaloids 는 증가하며, Reducing Sugar는 감소하는 傾向을 나타내었다.