• Journal of the Korean Society of Tobacco Science
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• v.16 no.2
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• pp.102-107
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• 1994

This study was conducted to transfer the potato virus Y-necrosis strain resistance from Nicotiana africana to Nicotiana tabacum (cv. NC82) N. africana was crossed with NC82, GErmination of the cotyledon stage, at which time most of the seedling died. However, surviving seedlings continued to grow normally. Chromosomes of the these interspecific self-sterile F1 hybrids were doubled by tissue culture. Amphidiploid of F1 hybrid was self-fertile. Starting with amphidipliod, a wystematic backcross (BC) program was set up with NC82 as recurrent parent. In the BC5C2 generation, the resistant plant was selected. This resistant line, KF8833-1, had 48 chromosome and secreting glandular trichomes. It flowered 2days later than NC82, and stalk height, leaves per plant, leaf length and leaf width were similar to those of NC82.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.649-654
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• 1995

This study was conducted to investigate the utility and agronomic characteristics and use of cytoplasmic. male-sterile (CMS) haploid plants derived from interspecific cross between (male-sterile NC82$\times$burley21) F$_1$ plant (Nicotiana tabacum L.) and Nicotiana africana. Abundant seeds of high germinability were obtained when Nicotiana tabacum (cytoplasmic male-sterile F$_1$ plants) is pollinated by Nicotiana africana. Most of seedlings died at the cotyledonary stage. The remaining seedlings are viable F$_1$ hybrids or maternal haploids that can be easily distinguished. Number of interspecific Fl hybrids and matermal haploids per capsule obtained from the interspecific cross between cytoplasmic male-sterile tobacco F$_1$ plants and N.africana yielded 2.2 and 0.5 plants, respectively. Out of 149 CMS haploid plants obtained from interspecific cross, 102 plants showed green type while the others were yellow type for leaf and stem. This results agreed with the genetic ratios expected among haploid plants from the F$_1$ hybrids heterozygous for two recessive genes of yellow color of burley tobacco plant. Out of 83 CMS haploid plants inoculated with TMV, 48 plants showed resistant, while the others was susceptible. It agreed with the expected genetic ratios for a single dominant gene of TMV resistance. CMS haploid plant will be useful as a source material for breeding of CMS doubled haploid lines

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.130-136
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• 1993

Protoplasts of palisade cells were aseptically isolated from leaves of Nicotiono apicona Merxm. by the one step enzymatic method. Efficiency of colony formation were depended on cell density and light condition during incubation, but an intensity of 47 ft-c during a period of 2 weeks after isolation of the protoplasts in the Nagata and Takebe's medium promoted the planting efficiency. Protoplast - derived calli of N. africana can be differentiated into shoot when cultured on Murashige and skoog's medium containing IAA(0.Smg/$\ell$) and zeatin(5.0mg/$\ell$).

• Journal of the Korean Society of Tobacco Science
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• v.16 no.1
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• pp.69-75
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• 1994

A new flue - cured tobacco variety KF113 was developed by Nicotiana africana method from a cross of NC82 and Coker 347 at the Suwon Experiment Station. It was tested in the 0fficial Variety Test in 1991-1992 and the flue - cured Regional Farm Test in 1992. KFl13 flowers 4 days later than WC82 (standard variety in Korea) and its harvestable leaves are 2 more than those of NC82. The leaf type and shape of KFl13 resembles Coker 347. It has high resistance to bacterial wilt (Pseudomonas solanacearum) and black shank (Phytophthora parasitica var. nicotianae), and is susceptible to tobacco mosaic virus. It should adapt well to the flue - cured production area and can reduce premature flowering under unfavorable weather conditions. Yield of KFl13 is 5% higher than that of NC82, and nearly equal in value per kg compare with NC82. This variety met acceptable standards for chemical and physical characteristics of cured leaf and for smoking taste evaluated by panel members in Korea Ginseng & Tobacco Research Institute.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.94-95
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• 1988

• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.82-88
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• 1999

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.1
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• pp.24-32
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• 1992

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single F t hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC 95, was used to develop the 30 dihaploid lines by anther culture(F1-ADH) and maternally-derived doubled haploid utilizing Nicotiana africana(F1-MDH), respectively. As compared with mid-parent, ADH lines showed increasing in number of leaves, delaying in days to flower and narrowing in leaf width. However, no significant differences in the other characters investigated were recognized. MDH lines also showed narrow leaf width, while no significant differences in the other characters were observed. The variations of the characters investigated were generally greater in ADH than MDH lines. MDH lines had higher plant height and shorter days to flower than ADH lines, while other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were plant height, leaf number, days to flower, and yield, while those showing relatively low value were leaf length, leaf width, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(F1-ADH, F1-MDH) and correlated responses associated with selection for yield were estimated. Plant height, leaf width, days to flower, percent reducing sugar and disease resistance would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.137-144
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• 1993

The present study was conducted to compare the efficiency of individual plant selection for resistance to bacterial wilt with 30 dihaploid lines derived by anther culture and Nicotiana africana method in Fl and F2 generation from a cross between Bright Yellow 4 (BY4) and NC95. F2 dihaploid lines were selected from bacterial wilt disease resistant plants screened under the naturally infested filed conditions. The populations of FB - ADH and FB MDH derived from F2 individual plants with bacterial wilt resistance showed higher resistance to the disease than the populations of Fl - ADH and Fl - MDH, respectively, and no difference for the disease resistance appeared by the haploid deriving method within a generation.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.1
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• pp.57-61
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• 1995

Black shank(Phytophthora parasitira roar. nicotianae) resistant burley tobacco (Nicotiana tabacum L.) germplasms, KB 104 and KB 106, were developed by Korea Ginseng & Tobacco Research Institute. KB 104 was developed from the single cross of Burley 21$\times$Newton 77, using a modified pedigree method. KB 104 was highly resistant to black shank, and its agronomic characteristics and chemical contents were comparable to those of Burley 21, and value per 10a was slightly higher than Burley 21, KB 106 is a maternally derived doubled haploid made by N. africana method from the single cross of Burley 21$\times$ Va 509. KB 106 was also highly resistant to black shank, had two more harvestable leaves per plant and flowered three days later than Burley 21 did. Total alkaloid and nicotine contents of KB 106 were significantly lower than those of Burley 21. But its nornicotine content was higher than Burley 21 5. Key wads : Burley tobacco germplasm, Black shank resistance.