• 한국자원식물학회지
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• 제33권2호
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• pp.138-152
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• 2020

본 연구는 강원도 태백시에 위치한 황지천의 장기모니터링을 위한 수변식물상 조사를 수행한 결과 65과 158속 203종 2아종 18변종으로 총 223분류군으로 확인되었다. 과별로 보면 국화과(30분류군), 벼과(19분류군), 장미과(15분류군), 콩과(12분류군) 순으로 높게 나타난다. 이 중 특산식물은 4분류군이다. 멸종위기 및 적색목록종은 출현하지 않았다. 식물구계학적 특정 식물은 IV등급 3분류군, III등급 4분류군, II등급 7분류군, I등급 8분류군으로 총 22분류군이다. 귀화식물은 28분류군으로 귀화율12.5%, 도시화지수 8.7%를 나타났다. 용도에 따른 분류는 식용 166종(74.4%), 약용 187종(83.8%), 향료용 9종(4.0%), 산업용 99종(44.3%), 관상용 106종(47.5%), 복원용 84종(37.6%), 사료/퇴비용 112종(50.2%), 용도 미상 5종(2.2%) 등으로 구분되었다. 이러한 조사 연구를 통해 하천정화시설의 설치 전과 후의 식물상 및 식생변화에 대한 척도를 확보하고 종합적인 관리를 위한 장기 모니터링 기초자료로 활용하고자 한다.

• 한국환경생태학회지
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• 제10권1호
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• pp.128-139
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• 1996

우리나라에서 비교적 잘 보존되어 있는 난대상록활엽수림의 12개 지역을 대상으로 이 지역에 주로 출현하는 상록수종과 생육하고 있는 희귀 및 멸종위기 식물과 귀화식물을 조사하였다. 난대상록활엽수림에 70% 이상 공통적으로 고르게 출현하는 수종으로는 녹나무과의 생달나무, 후박나무 등 28과 34속 37종류로 파아고디었으묘, 이들 수종은 상록활엽수림의 복원모형을 만드는데 중요한 지표가 되리라 판단된다. 면적 100m$^{2}$당 종수는 보길도가 5.511로 가장 높았으묘, 미조리(4.677), 맹선리(2.481) 등의 순이었다. 관찰된 희귀 및 멸종위기식물은 물푸레나무과의 이팝나무를 포함한 4종 등 총 24과 32종이었으며 보길도에서 13과12종 1변종 등 13종류로 가장 많은 희귀 및 멸종위기식물이 파악되었다. 귀화 및 도입식물의 경우 국화과는 7종, 콩과는 5종, 자리공과는 2종을 파악되었다. 이 외에 비름과, 자작나무과, 능소화과, 명아주과, 닭의장풀과, 측백나무과, 꿀풀과, 아욱과, 분꽃과, 소나무과, 마디풀과 및 낙우송과 등 12과는 각 1종씩 총 12종이 확인되었다. 보길도의 경우 낙우송과의 삼나무 등 총 9과 13속 13종으로 가장 많은 도입 또는 귀화식물이 식재되어 있음을 확인하였다. 또한 격리된 소규모 도서 생태계의 합리적인 보전을 위한 관리계획 수립의 필요성을 논하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1229-1236
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• 2012

Our previous studies showed that kisspeptin-10 (Kp-10) injected in vivo can markedly increase lipid anabolism in liver of quails. In order to investigate the direct effect of Kp-10 on lipid metabolism of hepatocytes in birds, cells were separated from embryos livers and cultured in vitro with 0, 100 and 1,000 nM Kp-10, respectively. The results showed that after 24 h treatment, cells viability was not affected by 100 nM Kp-10, but showed a mild decrease with 1,000 nM Kp-10 compared to the control cells. Based on the results of the cell viability, 100 nM dosage of Kp-10 was selected for the further study and analysis. Compared with control cells, total cholesterol (Tch) contents in 100 nM treated cells were increased by 51.23%, but did not reach statistical significance, while the level of triglyceride (TG), high density of lipoprotein-cholesterol (HDL-C) and low density of lipoprotein-cholesterol (LDL-C) were significantly increased. Real-time PCR results showed that ApoVLDL-II mRNA expression had a tendency to increase, genes including sterol regulatory element-binding protein-1 (SREBP-1), acetyl coenzyme A carboxylase ${\alpha}$ ($ACC{\alpha}$), carnitine palmitoyltransferase 1 (CPT1), 3-hydroxyl-3-methylglutaryl-coenzyme A reductases (HMGCR) and stearyl coenzyme A dehydrogenase-1 (SCD1) mRNA in hepatocytes were significantly down-regulated by 100 nM Kp-10. However, contrary to its gene expression, SREBP-1 protein expression was significantly up-regulated by 100 nM Kp-10. Some of the significant correlations in mRNA expression were found between genes encoding hepatic factors or enzymes involved in lipid metabolism in liver of birds. These results indicate that Kp-10 stimulates lipid synthesis directly in primary cultured hepatocytes of chickens.

• 한국결정성장학회지
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• 제7권4호
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• pp.648-657
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• 1997

합성원료로써 $Mg_3(PO_4)_2$와 $MgF_2$분말을 혼합한 후 백금밀폐용기를 사용하여 $1040^{\circ}C$에서 단일상의 wagnerite$(Mg_2PO_4F)$를 합성하였으며 이 결정상의 동질이상에 대하여 연구한 결과, 이미 발표된 연구 결과들과는 달리 $1255^{\circ}C$에서 단 한번의 가역적인 상전이가 관찰되었고, $1340^{\circ}C$에서 용융될 때까지 열분해 현상은 나타나지 않았으며, 이로써 2가 금속이온의 치환에 의해 여러 종류의 고용체를 합성해 낼 경우, 유약 및 플라스틱의 색상발현을 위한 안료로 사용될 수 있는 것으로 밝혀졌다. $Zn_4P_2O_8F_2$와 $Zn_3MgP_2O_8F_2$ wagnerite의 Zn 대신 2가 금속을 치환한 경우, 전반적으로 d값이 줄어들었으며 Co는 자주색, Ni은 금색, Cu는 녹색을 나타내었다. 또한 charge-coupled 치환을 시도한 결과, $A^{1+}A_3^{2+}X^{5+}X^{6+}O_8_F2$의 경우만 wagnerite가 형성되었다.

• 대한치과교정학회지
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• 제44권5호
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• pp.236-245
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• 2014

Objective: To evaluate the therapeutic effects of a preformed assembly of nickel-titanium (NiTi) and stainless steel (SS) archwires (preformed C-wire) combined with temporary skeletal anchorage devices (TSADs) as the sole source of anchorage and to compare these effects with those of a SS version of C-wire (conventional C-wire) for en-masse retraction. Methods: Thirty-one adult female patients with skeletal Class I or II dentoalveolar protrusion, mild-to-moderate anterior crowding (3.0-6.0 mm), and stable Class I posterior occlusion were divided into conventional (n = 15) and preformed (n = 16) C-wire groups. All subjects underwent first premolar extractions and en-masse retraction with preadjusted edgewise anterior brackets, the assigned C-wire, and maxillary C-tubes or C-implants; bonded mesh-tube appliances were used in the mandibular dentition. Differences in pretreatment and post-retraction measurements of skeletal, dental, and soft-tissue cephalometric variables were statistically analyzed. Results: Both groups showed full retraction of the maxillary anterior teeth by controlled tipping and space closure without altered posterior occlusion. However, the preformed C-wire group had a shorter retraction period (by 3.2 months). Furthermore, the maxillary molars in this group showed no significant mesialization, mesial tipping, or extrusion; some mesialization and mesial tipping occurred in the conventional C-wire group. Conclusions: Preformed C-wires combined with maxillary TSADs enable simultaneous leveling and space closure from the beginning of the treatment without maxillary posterior bonding. This allows for faster treatment of dentoalveolar protrusion without unwanted side effects, when compared with conventional C-wire, evidencing its clinical expediency.

• BMB Reports
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• 제38권2호
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• pp.225-231
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• 2005

Acid-labile subunit (ALS) is a component of the 150-kDa insulin-like growth factor-binding protein-3 (IGFBP-3) complex, which, by sequestering the majority of IGFs-I and -II and thereby prolonging the half-life of them in plasma, serves as a circulating reservoir of IGFs in mammalian species. A pGEX-2T plasmid and a baculovirus expression constructs harboring a coding sequence for glutathione-S transferase (GST)-porcine ALS (pALS) fusion protein were expressed in BL21(DE3) E. coli and Sf9 insect cells, respectively. The expressed protein was purified by glutathione or Ni-NTN affinity chromatography, followed by cleavage of the fusion protein using Factor Xa. In addition, pALS and hIGFBP-3 were also produced in small amounts in the Xenopus oocyte expression system which does not require any purification procedure. A 65-kDa pALS polypeptide was obtained following the prokaryotic expression and the enzymatic digestion, but biochemical characterization of this polypeptide was precluded because of an extremely low expression efficiency. The baculovirus-as well as Xenopus-expressed pALS exhibited the expected molecular mass of 85 kDa which was reduced into 75 and 65 kDa following deglycosylation of Asn-linked carbohydrates by Endo-F glycosidase, indicating that the expressed pALS was properly glycosylated. Moreover, irrespective of the source of pALS, the recombinant pALS and hIGFBP-3 formed a 130-kDa binary complex which could be immunoprecipitated by anti-hIGFBP-3 antibodies. Collectively, results indicate that an authentic pALS protein can be produced by the current expression systems.

• 한국환경과학회지
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• 제23권5호
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• pp.911-942
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• 2014

This study was carried out to investigate the flora of indigenous habitat of Donghae-si and Samcheok-si in Gangwon-do, Korea. The vascular plants were collected 18 times (from March to October 2011), and were identified as 804 taxa; 127 families, 445 genera, 720 species, 8 subspecies, 69 varieties, 5 forms and 2 hybrids. In the flora of this area, the calcicolous plants were 55 taxa; Cheilanthes argentea, Clematis serratifoliam, Quercus variabilis. Epipactis papillosa, etc. The endangered plants designated and protected by the wildlife protection law, the Ministry of Environment, Korean were counted to one taxon; Cymbidium macrorhizum. The red list of vascular plants according to IUCN valuation basis were examined, endangered species (EN); Prunus yedoensis and Cymbidium macrorhizum, Vulnerable species (VU); Juniperus chinensis, Pulsatilla tongkangensis, Diarthron linifolium and Swertia wilfordii, Near Threatened species (NT); Paeonia japonica, Allium senescens, Epipactis papillosa and Pogonia minor, Least Concern species (LC); Asplenium ruta-muraria, Platycladus orientalis, Monotropa hypopithys and Eleutherococcus divaricatus var. chiisanensis, etc., Data Deficient species (DD); Paeonia lactiflora var. trichocarpa, and Not Evaluate species (NE); Prunus choreiana, Panax ginseng and Polygonatum infundiflorum etc. Korean endemic plants of this area were 26 taxa; Aconitum pseudolaeve, Corydalis hirtipes, Vicia chosenensis, Salvia chanroenica and Hemerocallis hakuunensis, etc. The floristic regional indicator plants found in this area were 130 taxa comprising 11 taxa of grade V, 15 taxa of grade IV, 33 taxa of grade III, 15 taxa of grade II, 56 taxa of grade I. The naturalized plants were identified as 67 taxa and the percent of naturalized index (NI) was 8.3% of total 804 taxa vascular plants.

• Asian Pacific Journal of Cancer Prevention
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• 제15권12호
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• pp.4865-4869
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• 2014

Objective: This study evaluated the expression level of high mobility group box-B1 (HMGB-1) and matrix metalloproteinase-9 (MMP-9) in non-small cell lung cancer (NSCLC) inmorder to reveal any relation with development and prognosis. Methods: NSCLC and normal tissues were selected from 30 patients at age of 30-73, and used for RT-PCR and Western blot analyses of HMGB-1. A total of 100 paraffin embedded NSCLC tissues were also isolated from patients through surgical resection, and used for detection of HMGB-1 by immunohistochemistry. In addition, 50 samples were also applied for MMP-9 detection, and 30 normal tissues were considered as controls. Correlation analysis of HMGB-1 and MMP-9 was carried out by Pearsons correlation coefficient. Results: The average expression level of HMGB-1 in NSCLC patients was significantly higher than in normal lung tissues. In addition, patients in III-IV period exhibit significantly higher positive rate of HMGB-1 when compared with I-II period cases. Furthermore, a positive correlation with HMGB-1 was found in the expression of MPP-9. Conclusion: HMGB-1 was highly expressed in NSCLC, which may become a prognostic and predictive marker for NSCLC. Besides, MPP-9 was positively correlated with HMGB-1.

• The Journal of Advanced Prosthodontics
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• 제3권4호
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• pp.186-189
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• 2011

PURPOSE. Porcelain fused to metal (PFM) crowns provide the best treatment option for teeth that have a large or defective restoration. More than 20% of teeth with PFM crowns or bridges require non-surgical root canal treatment (NSRCT). This may be due to the effect of restorative procedures and the possible leakage of bacteria and or their by-products, which leads to the demise of the tooth pulp. Thus, this study was planned to compare the ability of the restorative materials to seal perforated PFM specimens. MATERIALS AND METHODS. The study evaluates the ability of amalgam, composite or compomer restorative materials to close perforated PFM specimen's in-vitro. Ninety PFM specimens were constructed using Ni-Cr alloys and feldspathic porcelain, and then they were divided into 3 groups: amalgam (A), composite + Exite adhesive bond (B) and compomer + Syntac adhesive bond (C). All the PFM samples were embedded in an acrylic block to provide complete sealing of the hole from the bottom side. After the aging period, each group was further divided into 3 equal subgroups according to the thermocycling period (one week for 70 cycles, one month for 300 cycles and three months for 900 cycles). Each subgroup was put into containers containing dye (Pelikan INK), one maintained at $5^{\circ}C$ and the other at $55^{\circ}C$, each cycle for 30 sec time. The data obtained was analyzed by SPSS, 2006 using one way ANOVA test and student t-test and significant difference level at (P<.01). RESULTS. The depth of dye penetration was measured at the interfaces of PFM and filling materials using Co-ordinate Vernier Microscope. The lowest levels of the dye penetration for the three groups, as well as subgroups were during the first week. The values of dye leakage had significantly increased by time intervals in subgroups A and C. CONCLUSION. It was seen that amalgam showed higher leakage than composite while compomer showed the lowest level of leakage.

• 한국수소및신에너지학회논문집
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• 제29권1호
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• pp.1-10
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• 2018

The amount of biogas increases as the amount of organic waste increases. Recently, biogas from organic waste have been made much efforts to utilize as a energy. In particular, the concentration of $CH_4$ and $CO_2$ generated from sewage sludge and livestock manure treatment are 60-70% and 30-35%, and $CH_4$ and $CO_2$ generated from food wastes are 60-80% and 20-40%. In case of landfill gas, $CH_4$ and $CO_2$ have a concentration of 40-60% and 40-60% respectively. Therefore, in order to use the biogas more widely, it is necessary to convert the biogas to methanol, LNG or DME. In this study, experiments were conducted to produce hydrogen and carbon monoxide through various biogas reforming reactions on $Ni/Ce-ZrO_2/Al2O3$ catalysts. The experiment of synthetic gas synthesis was carried out on a wide concentrations of methane and carbon dioxide, which were the major constituents of biogas from various organic wastes. The effect of $(O_2+CO_2)/CH_4$ (=R') on the yields of hydrogen and carbon monoxide, the conversion rate of methane and carbon dioxide was investigated. Also simulation for syngas synthesis on the $CO_2$ reforming of $CH_4$ was computed by employing total Gibbs free energy minimization method using PRO/II simulator, and compared with the experimental results on wet and dry reforming reaction of biogas.