• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.781-791
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• 2018

BACKGROUND: Glucosamine hydrochloride (GlcN HCl) has been shown to inhibit cell growth and matrix synthesis, but not with N-acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical growth factors (GF), or to a different metabolization of the two glucosamine derivatives. The aim of the present study was to evaluate the synergy between GlcN HCl, GlcNAc, and GF on proliferation and cartilage matrix synthesis. METHOD: Bovine chondrocytes were cultivated in monolayers for 48 h and in three-dimensional (3D) chitosan scaffolds for 30 days in perfusion bioreactors. Serum-free (SF) medium was supplemented with either growth factors (GF) $TGF-{\beta}$ ($5ng\;mL^{-1}$) and IGF-I ($10ng\;mL^{-1}$), GlcN HCl or GlcNAc at 1mM each or both. Six groups were compared according to medium supplementation: (a) SF control; (b) SF + GlcN HCl; (c) SF + GlcNAc; (d) SF + GF; (e) SF + GF + GlcN HCl; and (f) SF + GF + GlcNAc. Cell proliferation, proteoglycan, collagen I (COL1), and collagen II (COL2) synthesis were evaluated. RESULTS: The two glucosamines showed opposite effects in monolayer culture: GlcN HCl significantly reduced proliferation and GlcNAc significantly augmented cellular metabolism. In the 30 days 3D culture, the GlcN HCl added to GF stimulated cell proliferation more than when compared to GF only, but the proteoglycan synthesis was smaller than GF. However, GlcNAc added to GF improved the cell proliferation and proteoglycan synthesis more than when compared to GF and GF/GlcN HCl. The synthesis of COL1 and COL2 was observed in all groups containing GF. CONCLUSION: GlcN HCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis.

• YAKHAK HOEJI
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• v.46 no.6
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• pp.392-397
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• 2002

A simple and sensitive high performance liquid chromatographic method to quantitate ursodeoxycholic acid in crude drug pharmaceuticals was investigated. Ursodeoxycholic acid react with 2-bromoacetyl-6-methoxynaphthalene (Br-AMN) in the presence of triethylamine to form highly fluorescent derivative. The derivatization procedure was performed at 7$0^{\circ}C$ and completed within 30 min. The optimal wavelength of the fluorescence detector are λ$_{ex}$=300 nm and λ$_{em}$ = 460 nm. The LOD of the ursodeoxycholic acid was 25 ng/mι based on the S/N =3, and the LOQ was 80 ng/mι based on S/N = 10. Crude drug pharmaceuticals pretreated by solid phase extraction (Sep-pak $C_{18}$ cartridge) which were shown very good separation and recovery values for the compound.d.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2001.05a
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• pp.66-92
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• 2001

지난 및 년간 정부에서는 소각시설에서 배출되는 다이옥신 등 대기오염물질을 저감하기 위하여 많은 노력을 기울여 왔다. 먼저, '97년 7월 17일, 폐기물관리법 시행규칙을 개정하여 생활폐기물 소각시설에 대한 다이옥신 규제기준$^{1)}$ 을 '99년 7월 1일부터 기존 소각시설에 대해서는 0.5ng-TEQ/N㎥을 신설 소각시설에 대해서는 0.1ng-TEQ/N㎥으로 제정했으며, 또한 소각시설의 광역화, 신규 소각시설의 설치비용 및 기존 소각설비의 개ㆍ보수비용의 일부에 대한 국고지원, 소각처리 기술지원단에 의한 기술평가 및 자문활동, 다이옥신 공인측정ㆍ분석기관의 지정ㆍ고시, 생활폐기물 소각시설 설치ㆍ운영지침의 제정 등 짧은 기간임에도 불구하고 소각시설에서 배출되는 다이옥신을 저감하기 위하여 각종 정책이나 제도를 시행 또는 개선해왔다. (중략)

• Journal of the Korean Applied Science and Technology
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• v.29 no.3
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• pp.443-449
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• 2012

A investigation of electrochemical analysis of antibiotics Neomycin ($C_{23}H_{46}N_6O_{13}$) was searched using electrochemical square wave (SW) stripping and cyclic voltammetry (CV) using working sensor of the modified carbon nanotube combination electrodes, optimum diagnostic parameters were searched by anodic stripping, final conditions were attained to working range of 1.0-14.0 ng/L, detection limit (S/N) was found to be 0.6 ng/L. The developed method was discovered to be fitting in quality control in the food, pharmaceutical and other manufacturing sectors.

• Proceedings of the Korean Environmental Health Society Conference
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• 2002.04a
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• pp.84-90
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• 2002

In 1995 we licensed pyrolysis gas melting technology of indirect heating type (using kiln) from Siemens AG, and built its demonstration facility in 1998 at Clean-Park-East of Fukuoka City to demonstrate the technology for municipal solid waste (MSW). In 1997 we were awarded an order from Kanemura Co., Ltd. to build a pyrolysis gas melting and power generation plant, specifically for treating residue from car shredder. The latter was launched in 1998, and is currently in commercial operation. The operation of these plants have proven the following facts. (1) The system is capable for performing a stable operation with a wide variety of waste. (2) Pyrolysis is achieved steadily regardless of the variation in the quality of waste. (3) The system can be operated under low excess air ratio (1.2∼1.3). (4) The concentration of dioxins at the furnace outlet is 0.062ng-TEQ/㎥$\_$N/, and 0.002ng-TEQ/㎥$\_$N/, at the stack. (the value is corrected to dryO$_2$ 12％) (5) The purity of recovered metals exceeds 90%.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.6
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• pp.3882-3888
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• 2015

The present study was conducted to assess the relationship between obesity and abdominal obesity and Parathyroid hormone (PTH) in adults aged 50 or older (n=3,305) using 2011 Korean National Health and Nutrition Examination Survey (KNHANES) data, which represents national data in Korea. Key study results were as follows: First, the PTH levels increased significantly with an increase in Body mass index (BMI) (p=0.003), shown by sIAD levels after adjusted the variables that affect sIAD levels (age, gender, SBP, DBP, and 25(OH)D). These were $66.25{\pm}0.89ng/dL$ for Normal weight (BMI, < $23.0kg/m^2$), $67.83{\pm}1.07 ng/dL$ for Overweight (BMI, $23.0-24.9kg/m^2$), $70.71{\pm}0.94ng/dL$ for Obesity (BMI,${\geq}25.0kg/m^2$). Second, the PTH level of abdominal obesity group ($69.30{\pm}0.80ng/dL$) was significantly higher (p=0.017) than non-abdominal obesity group ($66.63{\pm}0.74ng/dL$). In conclusion, BMI and waist measurement are positively associated with the PTH levels in Korean adults.

• Journal of Environmental Health Sciences
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• v.40 no.1
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• pp.38-46
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• 2014

Objectives: This study was carried out in order to estimate atmospheric polycyclic aromatic hydrocarbon (PAH) concentrations and sources using pine needles as a passive air sampler (PAS) in urban (Pyeongtaek), semirural (Anseong) and rural (Jincheon) sites. Methods: One-year-old pine needles were collected for analysis of their PAH concentrations ($C_{p,n}g/g$ dry) at the end of December. PAHs concentrations in the ambient air ($C_a$, $ng/m^3$) were calculated with a $Log(C_p/C_a)-LogK_{oa}$ correlational equation. Results: PAHs concentrations in ambient air ($C_a$) were high, in the order of urban ($114.03ng/m^3$), semirural ($105.17ng/m^3$) and rural ($61.91ng/m^3$) sites. However, distributions of PAH isomer concentrations were very similar. PAHs of which molecular weight is smaller than 228.30 (AcPy, Acp, Flu, Phen, Ant, Flt, Pyr, BaA, Chry) made up most of the PAHs in the ambient air (96.6-98.5%). Conclusion: At urban, semirural and rural sites, it was concluded that the main source of PAHs in the ambient air ratio of each PAH isomer concentration was cars, especially diesel vehicles.

• Journal of Environmental Science International
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• v.4 no.3
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• pp.97-97
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• 1995

PCBs have been measured using GC-ECD, GC-MS, GC-ELCD, HPLC, TLC, NMR and Immunoassay. The analysis of PCBs using GC-ECD include the peak pattern method as none derivatization and the Perchlorination method as derivatization. This study was conducted to establish the perchlorination method with Sbcls from PCBs to decachlorinated biphenyl(DCB). The aroclor 1242 of PCBs was chlorinated and then, converted into the DCB which showed a single peak in GC-ECD chromatogram. The detection limit of DCB was 2pg. The quantification detection concentration of PCBs extracted with soxhlet was 0.5ng/g in the soil. PCBs were not detected in the suburban soil, but 174ng/g in the soil of industrial complex. Mean PCBs concentration of Shinchun stream at Kumho river and Jinchun stream at Nakdong river was calculated average 낙ngjg in 각e sediment. PCBs concentration in the sediment of Kumho river near 2-7km from conjunction with Nakdong river was average 154ng/g. PCBs concentration in the sediment of Nakdong river near conjunction with Kumho river was average 159ng/g.

• Environmental Engineering Research
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• v.13 no.4
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• pp.216-223
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• 2008

An efficient method for the simultaneous determination of eleven phenolic endocrine-disrupting chemicals (EDCs) present in wastewater influent samples was described. The 11 phenolic EDCs including alkylphenols, chlorophenols, and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring (GC/MS-SIM) following two work-up methods for comparison; isobutoxycarbonyl (isoBOC) derivatization and tert-butyldimethylsilyl (TBDMS) derivatization. The wastewater influent samples containing the 11 EDCs were adjusted to pH 2 with $H_2SO_4$ and then cleaned up with n-hexane. Next, they were subjected to solid-phase extraction (SPE) with XAD-4 resin and subsequently converted to isoBOC or TBDMS derivatives for sensitivity analysis with gas chromatography/mass spectrometry-selected ion monitoring (GC/MSSIM). Following isoBOC derivatization and TBDMS derivatization, the recoveries were 86.6-105.2% and 97.6-142.7%, the limits of quantitation (LOQ) for the 11 phenolic EDCs for SIM was 0.001-0.050 ng/mL and 0.003-0.050 ng/mL, and the SIM responses were linear with the correlation coefficient varying by 0.9717-0.9995 and 0.9842-0.9980, respectively. When these methods were applied to five selected wastewater influent samples, for isoBOC derivatization and TBDMS derivatization the ranges of concentration detected were 0.2-99.6 ng/mL and 0.4-147.4 ng/mL, respectively.

• Journal of the Korean Chemical Society
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• v.51 no.5
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• pp.412-417
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• 2007

A simple prepared paste electrode (PE) of DNA immobilized on a carbon nanotube was utilized for monitoring the antibacterial agent oxytetracycline (OTC), using square-wave anodic stripping voltammetry (SWASV) and cyclic voltammetry (CV). Given these conditions, SWASV and CV working ranges were observed within 1-10 ngL-1 OTC. In the SWASV and CV for OTC concentrations of 0.1 mgL-1, the relative standard deviations (n=15) were 0.068 and 0.067, respectively. At the optimized condition, the detection limit was found to be 0.4 ngL-1 OTC. This method was applied to the hatchery fish tissue.