• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.162-162
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• 2017

Atractylodes japonica and Atractylodes macrocephala belongs to Atractylodes genus and theirs roots are used as 'Baek-chul'(White atractylodes rhizome) in Korea Pharmacopoeia. 'Baek-chul' widely used in traditional herbal remedies in Asia. Concurrently, the 'Baek-chul' demand enormously increased in the market due to the growing interest in favoring health benefits but it is depend on import from China. Thus we conducted breeding research to solve the problems associated with domestically cultivated 'Baek-chul' such as low productivity and low disease tolence. We crossed A. japonica (female parent) and A. macrocephala (male parent) in the greenhouse in September 2000. the next season 119 crossed line seeds were collected and germinated in the green house. Among them we selected the good lines in the same season. then conducted replicated yield trials (RYT) in 2014 to local adaptability test (LAT) in 2015-2016. The results showed that the 'AJM16' line has beneficial charactersitcs like, increased plant height, large rhizome diameter, narrow leaf width compared with a 'Sang-chul' (check cultivar) and varied flower color from A. japonica (female parent). The line 'AJM16' was also exhibited decreased anthracnose symptoms(Colletotrichum gloeosporides Penz.). Simultaneously the root yield of AJM16 was 2,409 kg per are, which was increased 130% higher yield compared to 'Sang-chul' (check cultivar) at three various regions from 2015 to 2016. Therefore, we named AJM16 as new cultivar 'Man-chul'. According the results, we have developed a new Atractylodes spp. hybrid cultivar 'Macn-chul' with high yielding and disease tolerance by National Institute of Horticulture and Herbal Science, RDA, in 2016.

• Parasites, Hosts and Diseases
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• v.35 no.4
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• pp.223-232
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• 1997

Metagonimus miyatai n. sp. (Digenea: Heterophyidae) is described based on adult flukes collected from experimental dogs and hamsters fed with metacercariae encysted in the sweetfish, Plecoglossus altivelis, dace, Tribolodon hakonensis and T. taczanowskii, common fat-minnow. Morocco steindachneri pale chub, Zacco platypus, and dark chub, Zacco temmincki, and on those collected from naturally infected humans. The new species was morphologically compared with M. yokogawai and M. takahashii obtained from experimental animals fed with the sweetflsh and the crucian carp, Cnrossius caressius, respectively. The uterine loops of M. miyatai reached near the posterior end of the body through the space between the two testes, whereas those of M. yokogawai, occupied only the space between the acetabulum and anterior border of two testes. This uterine tubule distribution was similar to that of another closely related species, M. takahashii. However vitellaria of M. miyatai ended in front of the posterior end of the left testis, while those of M. takahcshii reached the posterior end of the left testis and ran it over. By raising M. miyatai as a new species, differentiation of M. yokogawai and M. takahashii became very clear. A key to the species of the genus Metagonimus in the Far East has been proposed.

• Parasites, Hosts and Diseases
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• v.55 no.5
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• pp.575-578
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• 2017

Aspidogastrid trematodes (Subclass Aspidogastrea) are a relatively small group with a characteristic adhesive disc and parasitize in a variety of cold-blooded hosts. Until now, only 2 species in the genus Cotylaspis, i.e., C. coreensis and C. sinensis, have been reported as the aspidogastrid trematode in the Republic of Korea (=Korea). In the present study, we intended to describe a species of aspidogastrids collected from the small intestine of the common carp, Cyprinus carpio, in a faunistic point of view. Total 51 specimens were collected from a carp caught in Nakdong-gang (River) on May 2015. Some of them were prepared as the specimens for light microscopic observations, and some others were prepared for SEM. They were slightly elongated without head lobes, $2,432{\times}840{\mu}m$ in average size, and had characteristic adhesive discs with 4 rows and 46 alveoli in average. The ovary was reniform and was located in the posterior-upper part of the body. The single testis was larger than the ovary and was located below the ovary. The uterus was coiled containing numerous eggs and distributed in the posterior 2/3 of the body. The vitellaria were follicular, and distributed from the mid-level of testis to near the posterior end. The morphological characters with dimensions of our specimens were closely identical with those of Aspidogaster ijimai previously described. A new aspidogastrid is added among the Korean trematode fauna by the present study.

• Korean Journal of Ichthyology
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• v.28 no.1
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• pp.52-56
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• 2016

A single specimen (700 mm in disc length) of Plesiobatis daviesi, belonging to the family Plesiobatididae, was firstly collected in the north-eastern coastal waters of Jejudo Island, Korea by using a bottom trawl on 24 October 2010. This species was characterized by having five pairs of gill openings, tail with one to three large spines, long snout length, long caudal fin, and pleated margin of nasal curtain. It is morphologically similar to Urolophus aurantiacus, but the former is distinguished from the latter by having longer caudal fin and snout length. We add P. daviesi to the Korean fish fauna and suggest the new Korean names, "Gin-kko-ri-huin-ga-o-ri-gwa", "Gin-kko-ri-huin-ga-o-ri-sok" and "Gin-kko-ri-huin-ga-o-ri" for the family, genus and species, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.4
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• pp.586-593
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• 2016

A new methanogen was isolated from an anaerobic digester using pig slurry in South Korea. Only one strain, designated KOR-1, was characterized in detail. Cells of KOR-1 were straight or crooked rods, non-motile, 5 to $15{\mu}m$ long and $0.7{\mu}m$ wide. They stained Gram-positive and produced methane from $H_2+CO_2$ and formate. Strain KOR-1 grew optimally at $38^{\circ}C$. The optimum pH for growth was 7.0. The strain grew at 0.5% to 3.0% NaCl, with optimum growth at 2.5% NaCl. The G+C content of genomic DNA of strain KOR-1 was 41 mol%. The strain tolerated ampicillin, penicillin G, kanamycin and streptomycin but tetracycline inhibited cell growth. A large fragment of the 16S rRNA gene (~1,350 bp) was obtained from the isolate and sequenced. Comparison of 16S rRNA genes revealed that strain KOR-1 is related to Methanobacterium formicicum (98%, sequence similarity), Methanobacterium bryantii (95%) and Methanobacterium ivanovii (93%). Phylogenetic analysis of the deduced mcrA gene sequences confirmed the closest relative as based on mcrA gene sequence analysis was Methanobacterium formicicum strain (97% nucleic acid sequence identity). On the basis of physiological and phylogenetic characteristics, strain KOR-1 is proposed as a new strain within the genus Methanobacterium, Methanobacterium formicicum KOR-1.

• The Korean Journal of Mycology
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• v.43 no.4
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• pp.224-230
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• 2015

A re-study was conducted based on morphological characters of 39 specimens known as Mycena stylobates, collected from 33 areas, Mt. Chiack, Kangwon-do, etc. from 1988 to 2015 and deposited in the Herbarium Conservation Center of the National Institute of Agricultural Sciences (HCCN). All specimens cited here were confirmed as a new to science, and designated as "Mycena yangsupensiae" which was described and illustrated in detail. Mycelial growth of four cultures from the fruiting bodies of Mycena sp. (KACC54181, KACC54182, KACC54183, and KACC54184) was high on the potato dextrose agar (PDA), at $25^{\circ}C$. These four taxa were confirmed as the same species, Mycena yangsupensiae, by the internal transcribed spacer (ITS) sequencing analysis. All collections cited here are deposited in HCCN, Suwon.

• Korean journal of applied entomology
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• v.54 no.3
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• pp.169-177
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• 2015

The Korean species of the genera Cremastus Gravenhorst, 1829 and Pristomerus Curtis, 1836 (Hymenoptera: Ichneumonidae) are studied. Here we report three species, Cremastus crassitibialis Uchida, 1940, Pristomerus rufiabdominalis Uchida, 1928 and P. scutellaris Uchida, 1932, new to South Korea. Among them genus Cremastus is recorded for the first time from South Korea. The number of Korean Cremastinae is now 11 species in four genera including the three newly recorded species. A key to the genera of Korean Cremastinae, diagnosis of newly recorded species and photographs of redescriptions characters are provided.

• Archives of Pharmacal Research
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• v.19 no.2
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• pp.153-159
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• 1996

A new streptothricin family antibiotic producing Streptomyces spp. SNUS 8810-111 was isolated from a soil sample. Study of its morphological and physiological characters indicated that the antibiotic producing organism was a Streptomyces spp. Taxonomical studies suggested that the organism might belong to the genus streptomyces gougeroti. The organism produced antibiotics most in calcium carbonate-tryptic soy broth. The active principles were recovered from the broth with a cation exchange resin and eluted from the resin with HCI. Cellulose column chromatography gave two active principles.$^1H-^1H$ Homo-COSY study on the first compound revealed four structural components. Total hydrolysis of the antibiotic with HCI allowed isolation of $\beta-lysine$. From these data the antibiotic was found to be streptothricin D. The other compound showed one additional signal in the .$^1H$NMR and the $^{13}C$ NMR spectra. The signal was from a methyl group attached to a nitrogen atom. Comparison of the NMR signals with those of streptothricin D suggested that the compound was N-methyl-streptothricin D which was a new compound in the family of streptothricin antibiotics.

• Journal of Microbiology and Biotechnology
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• v.21 no.7
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• pp.667-674
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• 2011

The gene for esterase (rEst1) was isolated from a new species of genus Rheinheimera by functional screening of E. coli cells transformed with the pSMART/HaeIII genomic library. E. coli cells harboring the esterase gene insert could grow and produce clear halo zones on tributyrin agar. The rEst1 ORF consisted of 1,029 bp, corresponding to 342 amino acid residues with a molecular mass of 37 kDa. The signal P program 3.0 revealed the presence of a signal peptide of 25 amino acids. Esterase activity, however, was associated with a homotrimeric form of molecular mass 95 kDa and not with the monomeric form. The deduced amino acid sequence showed only 54% sequence identity with the closest lipase from Cellvibrio japonicus strain Ueda 107. Conserved domain search and multiple sequence alignment revealed the presence of an esterase/ lipase conserved domain consisting of a GXSXG motif, HGGG motif (oxyanion hole) and HGF motif, typical of the class IV hormone sensitive lipase family. On the basis of the sequence comparison with known esterases/ lipases, REst1 represents a new esterase belonging to the class IV family. The purified enzyme worked optimally at $50^{\circ}C$ and pH 8, utilized pNP esters of short chain lengths, and showed best catalytic activity with p-nitrophenyl butyrate ($C_4$), indicating that it was an esterase. The enzyme was completely inhibited by PMSF and DEPC and showed moderate organotolerance.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.1
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• pp.62-64
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• 2003

Recently, relatively large amount of a new species of crab belonging to genus Chionoecetes that has intermediate characteristics between red-tanner crab (Chionoecetes japonicus) and queen crab (C. opilio) was caught the East Sea of Korea. The new species of crab was tentatively named Neodo-Daege. Amino acid and proximate compositions of the edible meat of red-tanner crab and Neodo-Daege were determine. The proximate composition of the meats of Neodo-Daege were $79.2{\%}$ in moisture, $17.4{\%}$ in protein, $0.6{\%}$ in lipid, and $1.5{\%}$ in ash. Neodo-Daege was higher in protein and lipid contents, and lower in moisture and ash contents than those of red-tanner crab. The total amino acid contents in the meat of Neodo-Daege were higher than those of red-tanner crab, but the profile of amino acids was similar to one another. Among amino acids, glutamic acid, arginine, aspartic acid, lysine and glycine were major components of the crabs.