• Toxicological Research
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• v.14 no.2
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• pp.261-271
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• 1998

The acute and subacute toxicity of New Wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk were investigated in S.D. rats. In intraperitoneal acute toxicity test, rats were injected intraperitoneally with five dosages of 0, 500, 710, 1,000, 1,410 and 2,000 mg/kg. Body weights were significantly decreased at 500 and 710 mg/kg dose group in male and abnormal autopsy findings were founded in both sexes at all dose. Intraperitoneal $LD_{50}$ of NSCH was 1,088.3 mg/kg in male and 1159.3 mg/kg in female rats. In the subacute toxicity study, NSCH was administrated orally to both sexes of rats for 4 weeks as several doses(0, 320, 800, and 2,000 mg/kg). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data strongly suggest that no observed adverse effect level of NSCH might be over 2,000 mg/kg/day in this study.

• Toxicological Research
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• v.14 no.2
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• pp.273-283
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• 1998

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material qf musk, to beagle dogs of both sexes were per-formed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with single dose of 2,000 mg/kg and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 mg/kg/day (low dosage group), 400 mg/kg/day (middle dosage group), 1,000 mg/kg/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Ad-ministration (l996. 4. 16). $LD_{50}$ value for beagle dogs was more than 2,000 mg/kg per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.