• Korean journal of applied entomology
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• v.61 no.4
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• pp.675-688
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• 2022

Produced by steaming mature silkworms, HongJam is a natural functional food with various health-promoting effects. The current standard HongJam production protocol involves freezing and freeze-drying steamed mature silkworms for convenient long-term storage and/or selling it to customers. However, freeze-drying HongJam requires a range of processes and costs, which have contributed to its high resale value. In this study, we found that the cost of manufacturing HongJam powder could be reduced by homogenizing steamed mature silkworms using a high-speed blender and digital homogenizer, followed by spray drying. After the homogenized HongJam solution was digested by directly adding food-graded proteases, food-graded protease-digested homogenized HongJam solution was spray-dried. Food-graded digested protease or non-digested homogenized HongJam solutions could be used to produce food for special medical use for patients with general or specific diseases. This more efficient HongJam processing protocol proposed in this study can facilitate the development of sericulture farms and related industries by reducing the production costs of HongJam and its associated products.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.4
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• pp.265-272
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• 2022

The emergence and dissemination of carbapenemase-producing Enterobacteriaceae (CPE), particularly the Klebsiella pneumoniae carbapenemase-2 (KPC-2) producing Klebsiella pneumoniae, has been rapidly increasing worldwide and is becoming a serious public health threat. Since the epidemiology and characteristics of these KPC-2-producing K. pneumoniae vary according to the region and period under consideration, this study investigated the prevalence of carbapenemases and the epidemiological relationship of 78 carbapenem-resistant K. pneumoniae (CRKP) isolated from a tertiary hospital in Daejeon, from March 2017 to December 2020. The antimicrobial susceptibility tests were identified using the disk-diffusion method. PCR and DNA sequencing were used to determine the carbapenemase genes. In addition, molecular epidemiology was performed by multilocus sequence typing (MLST). Among the 78 CRKP isolates, 35 isolates (44.9%) were carbapenemase-producing K. pneumoniae (CPKP) and the major carbapenemase type was KPC-2 (30 isolates, 85.7%). The New Delhi metallo-enzyme-1 (NDM-1) and NDM-5 were identified in 4 isolates (11.4%) and 1 isolate (2.9%), respectively. Multilocus sequence typing (MLST) analysis showed 10 sequence types (STs) and the most prevalent ST was ST307 (51.4%, 18/35). All the ST307 isolates were KPC-2-producing K. pneumoniae and were multidrug-resistant (MDR). In addition, ST307 has gradually emerged during a four-year period. These findings indicate that continuous monitoring and proper infection control are needed to prevent the spread of KPC-2-producing K. pneumoniae ST307.

• Journal of Life Science
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• v.33 no.9
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• pp.754-765
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• 2023

Exosomes are a type of extracellular vesicle containing proteins and messenger and microRNAs; they are secreted by all cell types. Once released, exosomes are selectively taken up by other cells adjacent or at a distance, releasing their contents and reprogramming the target cells. Since exosomes are natural vesicles produced by cells as small sizes, it is generally accepted that exosomes have a non-toxic nature and non-immunogenic behaviors. Recently, exosomes have elicited scientific attention as drug delivery vehicles to the central nervous system. The central nervous system has a blood-brain barrier that makes it difficult for drugs to penetrate. Thus, the blood-brain barrier has been a major obstacle to the development of drugs for treating neurodegenerative diseases. However, accumulating evidence suggests that exosomes can cross the blood-brain barrier primarily through transcytosis. Consequently, exosomes are expected to become a new delivery vehicle that can cross the blood-brain barrier and deliver drugs into the brain parenchyma. In addition, since different types of exosomes are secreted depending on the cell type and disease state, exosomes can also be utilized as biomarkers for the diagnosis of diseases in the central nervous system. In this review, we summarized recent research trends on exosomes, including clinical trials as biomarkers and treatment options for diseases in the central nervous system.

• Journal of Intelligence and Information Systems
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• v.29 no.3
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• pp.19-36
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• 2023

As advances in information and communication technology have made it easier for anyone to produce and disseminate information, a new problem has emerged: fake news, which is false information intentionally shared to mislead people. Initially spread mainly through text, fake news has gradually evolved and is now distributed in multimedia formats. Since its founding in 2005, YouTube has become the world's leading video platform and is used by most people worldwide. However, it has also become a primary source of fake news, causing social problems. Various researchers have been working on detecting fake news on YouTube. There are content-based and background information-based approaches to fake news detection. Still, content-based approaches are dominant when looking at conventional fake news research and YouTube fake news detection research. This study proposes a fake news detection method based on background information rather than content-based fake news detection. In detail, we suggest detecting fake news by utilizing related video information from YouTube. Specifically, the method detects fake news through CNN, a deep learning network, from the vectorized information obtained from related videos and the original video using Doc2vec, an embedding technique. The empirical analysis shows that the proposed method has better prediction performance than the existing content-based approach to detecting fake news on YouTube. The proposed method in this study contributes to making our society safer and more reliable by preventing the spread of fake news on YouTube, which is highly contagious.

• KIPS Transactions on Software and Data Engineering
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• v.12 no.8
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• pp.355-364
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• 2023

As advanced cyber threats continue to increase in recent years, it is difficult to detect new types of cyber attacks with existing pattern or signature-based intrusion detection method. Therefore, research on anomaly detection methods using data learning-based artificial intelligence technology is increasing. In addition, supervised learning-based anomaly detection methods are difficult to use in real environments because they require sufficient labeled data for learning. Research on an unsupervised learning-based method that learns from normal data and detects an anomaly by finding a pattern in the data itself has been actively conducted. Therefore, this study aims to extract a latent vector that preserves useful sequence information from sequence log data and develop an anomaly detection learning model using the extracted latent vector. Word2Vec was used to create a dense vector representation corresponding to the characteristics of each sequence, and an unsupervised autoencoder was developed to extract latent vectors from sequence data expressed as dense vectors. The developed autoencoder model is a recurrent neural network GRU (Gated Recurrent Unit) based denoising autoencoder suitable for sequence data, a one-dimensional convolutional neural network-based autoencoder to solve the limited short-term memory problem that GRU can have, and an autoencoder combining GRU and one-dimensional convolution was used. The data used in the experiment is time-series-based NGIDS (Next Generation IDS Dataset) data, and as a result of the experiment, an autoencoder that combines GRU and one-dimensional convolution is better than a model using a GRU-based autoencoder or a one-dimensional convolution-based autoencoder. It was efficient in terms of learning time for extracting useful latent patterns from training data, and showed stable performance with smaller fluctuations in anomaly detection performance.

• Journal of Life Science
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• v.33 no.8
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• pp.612-622
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• 2023

T-cell deficiency may occur in various clinical conditions including congenital defects, cell/organ transplantation, HIV infection and aging. In this regard, the development of artificial thymus has recently been attracting much attention. To achieve this aim, the development of techniques for 3D culture of thymic stromal cells is necessary because thymocytes grown only in a 3D thymic microenvironment can be differentiated fully to become mature, immunocompetent T cells; the same cannot be achieved for thymocytes grown in 2D. This study aimed to develop a nanotechnology-based 3D culture technique using polymeric scaffolds for thymic epithelial cells (TECs), the main component of thymic stromal cells. Scanning electron microscopic observation revealed that the pores of both PCL and PCL/PLGA scaffolds were filled with TECs. Interestingly, TECs grown in 3D on polydopamine-coated scaffolds exhibited enhanced cell attachment and proliferation compared to those grown on non-coated scaffolds. In addition, the gene expression of thymopoietic factors was upregulated in TECs cultured in 3D on polydopamine-coated scaffolds compared to those cultured in 2D. Taken together, the results of the present study demonstrate an efficient 3D culture model for TECs using polymeric scaffolds and provide new insights into a novel platform technology that can be applied to develop functional, biocompatible scaffolds for the 3D culture of thymocytes. This will eventually shed light on techniques for the in vitro development of T cells as well as the synthesis of artificial thymus.

• Journal of Platform Technology
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• v.11 no.2
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• pp.39-53
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• 2023

Non-installation program (hereinafter referred to as "portable program") is a program that can be used without an installation process, unlike general software. Since there is no separate installation process, portable programs have high mobility and are used in various ways. For example, when initial setup of multiple PCs is required, a portable program can be stored on one USB drive to perform initial setup. Alternatively, when a problem occurs with the PC and it is difficult to boot normally, Windows PE can be configured on the USB drive and portable programs can be stored for PC recovery. And the portable program does not directly affect PC settings, such as changing registry values, and does not leave a trace. This means that the portable program has high security. If a portable program is deleted after using it, it is difficult to analyze behavior in a general way. If a user used a portable program for malicious behavior, analysis in a general way has limitations in collecting evidence. Therefore, portable programs must have a new way of behavioral analysis that is different from ordinary installation software. In this paper, after installing the Windows 10 operating system on a virtual machine, we proceed with the scenario with a portable program of Opera and Notepad++. And we analyze this in various ways such as file analysis of the operating system and memory forensics, collect information such as program execution time and frequency, and conduct specific behavioral analysis of user.

• Journal of the Daesoon Academy of Sciences
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• v.45
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• pp.103-151
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• 2023

This study aims to examine the International Development Cooperations (IDCs) of Daesoon Jinrihoe and discuss the future prospects of these programs in terms of Daesoon Thought. The international community calls for the active participation of various donors, and this is not limited to only traditional donors such as governments, public institutions, and multilateral organizations. In this regard, the role of Faith-Based Organizations (FBOs) and Non-Governmental Organizations (NGOs) for religious communities is recognized as important. Daesoon Jinrihoe has systematized its IDCs by expanding its overseas services to implement the three major works with the official establishment of the Daejin International Volunteers Association (DIVA) in 2013. In addition to these activities, Daesoon Jinrihoe was selected by Korea International Cooperation Agency (KOICA) in 2022. As a new religion, Daesoon Jinrihoe does not provide as much support via FBOs when compared to other traditional religions (Christianity, Buddhism, Catholicism, etc.), but it is able to conduct strategic IDCs by utilizing its expertise and capabilities (education, healthcare, etc.). In fact, the nature and content of the projects carried out by these FBOs and Daesoon Jinrihoe are similar, and the results have been remarkable considering that these programs are still in their infancy. Above all, Daesoon Jinrihoe's foreign aid is more meaningful because it does not have a religious purpose such as missionary work, and it is funded by donations from believers. In carrying out IDCs, it is necessary to promote the professionalism of IDCs based on the strategic linkage of the three major works (charity aid, social welfare, and education). It is also necessary to recognize the value and spirit of IDCs for peace and coexistence based on mutual beneficence.

• Nuclear Engineering and Technology
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• v.55 no.8
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• pp.2935-2940
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• 2023

A polystyrene phantom was developed following the guidance of the International Atomic Energy Association (IAEA) for gamma knife (GK) quality assurance. Its performance was assessed by measuring the absorbed dose rate to water and dose distributions. The phantom was made of polystyrene, which has an electron density (1.0156) similar to that of water. The phantom included one outer phantom and four inner phantoms. Two inner phantoms held PTW T31010 and Exradin A16 ion chambers. One inner phantom held a film in the XY plane of the Leksell coordinate system, and another inner phantom held a film in the YZ or ZX planes. The absorbed dose rate to water and beam profiles of the machine-specific reference (msr) field, namely, the 16 mm collimator field of a GK Perfexion^TM or Icon^TM, were measured at seven GK sites. The measured results were compared to those of an IAEA-recommended solid water (SW) phantom. The radius of the polystyrene phantom was determined to be 7.88 cm by converting the electron density of the plastic, considering a water depth of 8 g/cm². The absorbed dose rates to water measured in both phantoms differed from the treatment planning program by less than 1.1%. Before msr correction, the PTW T31010 dose rates (PTW Freiberg GmbH, New York, NY, USA) in the polystyrene phantom were 0.70 (0.29)% higher on average than those in the SW phantom. The Exradin A16 (Standard Imaging, Middleton, WI, USA) dose rates were 0.76 (0.32)% higher in the polystyrene phantom. After msr correction factors were applied, there were no statistically significant differences in the A16 dose rates measured in the two phantoms; however, the T31010 dose rates were 0.72 (0.29)% higher in the polystyrene phantom. When the full widths at half maximum and penumbras of the msr field were compared, no significant differences between the two phantoms were observed, except for the penumbra in the Y-axis. However, the difference in the penumbra was smaller than variations among different sites. A polystyrene phantom developed for gamma knife dosimetry showed dosimetric performance comparable to that of a commercial SW phantom. In addition to its cost effectiveness, the polystyrene phantom removes air space around the detector. Additional simulations of the msr correction factors of the polystyrene phantom should be performed.

• Journal of Korea Entertainment Industry Association
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• v.13 no.3
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• pp.269-278
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• 2019

This study is intended to present the direction of art mask design development and help people's life care by producing an art mask with the motif of the characteristic of abjection concept. It went through the definition and characteristics of abject and abjection focusing on previous research, professional books and art works. The characteristics of abjection based on theoretical research are to express death with dead bodies and skulls, including the dismantling of ideal beauty standards, the expression of fragmented bodies, and the expression of sick and alienated bodies. This researcher produced 6 research works by combining the fascinating abjection, induced by ugly and disgusting abject, to design factors. These works fragmented each part of the facial form by the cutting and dismantling, and made viewers feel the abjection by giving a grotesque deformation. The results obtained through the research works are as follows. First, It was found that the abjection characteristics-applied works was helpful in the treatment of the mental wound of modern people. Second, the production of works based on the abjection characteristics paved the way to expand the scope of art mask design concepts and establish the new design ideas. Third, the application of the motif using the abjection characteristics to the art mask design showed that the use of various objects could express creative designs and secure diversity in using materials. In the future, this researcher hopes that such research will be used as the basic data of the follow-up study on art mask design, and it will help develop art mask designs.