• Journal of Pharmacopuncture
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• v.17 no.2
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• pp.41-45
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• 2014

Objectives: This study was performed to analyze the single-dose toxicity of neutral natured blood stasis pharmacopuncture extracts. Methods: All experiments were conducted at Biotoxtech, an institution authorized to perform non-clinical studies, under the regulations of Good Laboratory Practice (GLP). Sprague-Dawley rats were chosen for the pilot study. Doses of neutral natured blood stasis pharmacopuncture extracts, 0.1, 0.5 and 1.0 mL, were administered to the experimental group, and the same doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: In all 4 groups, no deaths occurred, and the neutral natured blood stasis pharmacopuncture extracts administered by intramuscular (IM) injection was over 1.0 mL/animal. No significant changes in the body weights between the control group and the experimental group were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any organs or tissues. Conclusion: The above findings suggest that treatment with neutral natured blood stasis pharmacopuncture extracts is relatively safe. Further studies on this subject should be conducted to yield more concrete evidence.

• Journal of Korean Medicine Rehabilitation
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• v.20 no.1
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• pp.13-26
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• 2010

Objectives : This study was designed to evaluate neuroprotective effects of Neutral Pharmacopuncture for Blood Stasis(NPBS) into SP10 and Tangguisusangami-tang (dangguixusanjiawei-tang)(TGT) in the experimental Traumatic Brain Injury(TBI)rats. Methods : Male rats were divided into 4 groups. Group I was no treatment after TBI. Group II was treatment with NPBS into SP10 after TBI. Group III was treatment with TGT after TBI. Group IV was NPBS into SP10 and TGT after TBI. The author carried out neurological motor behavioral, histological assessment test. Results : 1. In neurological motor behavior tests, motor and cognitive function recovery was significantly increased in the Group II, III, IV. Also Group IV was increased as compared with Group II, III. 2. In BAX expression, according to priority Group IV, III, II, I were decresed in 7 and 14 days later. Especially Group IV was significantly decreased in 14 days later. 3. In BCL-2 expression, Group IV was increased slightly in 7 days later. Most incresed expression was experimented in the Group IV in 14 days later. 4. In TUNEL expression, IV was decreased as compared with each Group I, II, III in 7 days later. Group IV, III were decreased as compared with each Group I, II, III in 14 days later. Conclusions : According to the results, NPBS and TGT can inhibit apoptosis of cells after TBI in rats by contol of BAX and BCL-2, TUNEL expression. And also can help neurological motor behavioral function.

• Journal of Pharmacopuncture
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• v.6 no.3
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• pp.57-63
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• 2003

Background : This study was to investigate existing studies on the mushroom Phellinus linteus for possible applications in treating hard to cure diseases and uses in herbal acupuncture as the above is known to have an anti-cancer effects and stimulating the immune system. Results : Based on the literary consideration, the following results were obtained : 1. Proteins and polysaccharides of the Class Basidiomycetes activate the host immune system for anti-cancer action without known side effects, thus it can be given for the prevention and treatment of cancer as a supplement. Phellinus linteus mushroom showed the most significant effects. 2. The Phellinus linteus mushroom is a fungi in the family Hymenochaetaceae and Phellinus, under the class Basidiomycetes. Phellinus linteus can be often found on the trunk of mulberry trees and other latifoliate trees. 3. The characteristics of Phellinus linteus are sweet, neutral in temperature, non-toxic, dissipate the stagnated blood, stop bleeding, invigorate the blood circulation and remove stasis, and nourish qi. It has been used for treating continuous menstrual discharge among the female patients and removing masses in the abdomen to name a few. 4. Phellinus linteus contains agaric acid, fatty acid, C23 and other numerous enzymes. 5. Phellinus linteus is known to have anti-cancer and anti-oxidant effects, stimulation of the immune system, as well as anti-inflammatory effects, inhibition of angiogenesis, and analgesic actions.