• Animal Bioscience
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• 제37권6호
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• pp.1077-1084
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• 2024

Objective: The present work was conducted to evaluate suitable variables and develop prediction equations using chemical composition and physical characteristics for estimating standardized ileal digestibility (SID) of lysine (Lys) in full-fat soybeans (FFSB). Methods: The chemical composition and physical characteristics were determined including trypsin inhibitor activity (TIA), urease activity (UA), protein solubility in 0.2% potassium hydroxide (KOH), protein dispersibility index (PDI), lysine to crude protein ratio (Lys:CP), reactive Lys:CP ratio, neutral detergent fiber, neutral detergent insoluble nitrogen (NDIN), acid detergent insoluble nitrogen (ADIN), acid detergent fiber, L* (lightness), and a* (redness). Pearson's correlation (r) was computed, and the relationship between variables was determined by linear or quadratic regression. Stepwise multiple regression was performed to develop prediction equations for SID of Lys. Results: Negative correlations (p<0.01) between SID of Lys and protein quality indicators were observed for TIA (r = -0.80), PDI (r = -0.80), and UA (r = -0.76). The SID of Lys also showed a quadratic response (p<0.01) to UA, NDIN, TIA, L*, KOH, a* and Lys:CP. The best-fit model for predicting SID of Lys in FFSB included TIA, UA, NDIN, and ADIN, resulting in the highest coefficient of determination (R² = 0.94). Conclusion: Quadratic regression with one variable indicated the high accuracy for UA, NDIN, TIA, and PDI. The multiple linear regression including TIA, UA, NDIN, and ADIN is an alternative model used to predict SID of Lys in FFSB to improve the accuracy. Therefore, multiple indicators are warranted to assess either insufficient or excessive heat treatment accurately, which can be employed by the feed industry as measures for quality control purposes to predict SID of Lys in FFSB.

• 한국식품과학회지
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• 제21권2호
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• pp.269-275
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• 1989

현율무, 율무쌀, 율무겨 그리고 현염주를 시료로 하여 일반성분, 총 식이섬유 함량, acid detergent fiber 함량, lignin 함량, 수분 결합력, 아미노산 조성 및 중성지질 조성 등을 살펴보았다. 그 결과, 율무쌀의 총 식이섬유량은 2.70% 였고, 현율무는 3.86% 였으며 현염주는 4.33% 그리고 율무겨는 13.12%였다. Acid detergent fiber 양은 율무쌀이 1.81%로 가장 낮았고 현율무, 현염주는 각각 3.45%, 3.26%로 비슷한 값이었다. 율무겨에서 추출한 총 식이섬유와 acid detergent fiber 의 수분 결합력을 측정하여 비교한 바 총 식이섬유의 수분 결합력은 $2.63{\pm}0.02g\;H_2O/g\;TDF$로 매우 낮았고, acid detergent fiber의 수분 결합력은 $5.89{\pm}0.152g\;H_2O/g\;ADF$였다. 율무쌀, 현율무, 현염주 그리고 율무겨의 아미노산 조성은 glutamic acid 함량이 가장 높았고 다음이 leucine 이었으며, 율무쌀의 경우 leucine 의 chemical score는 189로 매우 높았으며 반면에 lysine의 chemical score는 22-32 사이로 매우 낮아 율무, 염주의 제한 아미노산이 되었다. 지질성분은 중성지질이 80.89%-96.55% 범위로 대부분을 차지했고, 당지질이 2.35%-7.48% 사이로 현염주의 당지질 함량이 가장 낮았다. 인지질은 함량이 매우 낮아서 1.10%-2.12% 범위였다. 각 지질 성분의 지방산 조성은 palmitic acid, oleic acid 그리고 linoleic acid 였으며 현염주를 제외하고는 조성상에 크게 차이가 나지 않았다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1427-1432
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• 2015

The experiment was conducted to determine nutritive values of caramba (Lolium multiflorum cv. caramba) fresh, silage and hay by in vivo and in vitro methods. There was a statistically significant difference (p<0.01) in crude protein content value between fresh caramba (12.83%) and silage (8.91%) and hay (6.35%). According to results of experiment, the crude fiber, neutral detergent fiber, acid detergent fiber (ADF), acid detergent lignin contents of the three forms of caramba varied between 30.22% to 35.06%, 57.41% to 63.70%, 35.32% to 43.29%, and 5.55% to 8.86% respectively. There were no significant differences between the three forms of caramba in digestibility of nutrients and in vivo metabolizable energy (ME) values (p>0.05). However, the highest $ME_{CN}$ (ME was estimated using crude nutrients) and $ME_{ADF}$ values were found in fresh caramba (p<0.01). As a result, it could be said that, there were no differences between the three forms of caramba in nutrient composition, digestibility and ME value, besides drying and ensiling did not affect digestibility of hay. Consequently, caramba either as fresh, silage or hay is a good alternative source of forage for ruminants.

• Asian-Australasian Journal of Animal Sciences
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• 제2권1호
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• pp.27-34
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• 1989

Different fractions of maize plant and whole mixed fodder were analysed for their chemical composition and dry matter digestibility (DMD). Highest crude protein (CP) values were found in leaves as compared to the other portions. Younger plants contained more CP as compared to the matured ones. The crude fiber (CF) content of various fractions of the plant ranged between 19.12 to 35.60% with maximum values in the bottom portion of the stem. Matured plants contained more CF. The analysis of cell wall constituents indicated that the maximum values for neutral detergent fiber (NDF) were found in the bottom portion and in the whole mixed plant. The highest levels of acid detergent fiber (ADF) were observed in bottom fraction followed by whole mixed plant, whereas the other plant fractions did not show any differences. Variation in acid detergent lignin (ADL) values existed in different fractions of the plant and the lowest were in the top portion of the stem. Although there existed a variation in the mineral composition of different fractions of the plants, the results were non significant. Maximum DMD was found in leaves followed by the whole mixed plant, middle and bottom portion of the stem. The values of DMD were higher in younger plants as compared to the matured ones. It may be concluded that younger plants and the upper portion of the plants have a higher nutritive value as compared to the matured plants and lower portion of the plants.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.53-62
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• 2004

The aim of this study was to predict the energy value and dynamic degradation of roughage in Taiwan using the $Daisy^{(R)}$. in vitro fermentation method to provide information on one of the very important nutrients for ration formulation. The second objective was to study the effects of Aspergillus oryzae (AFE) inclusion on nutrient utilization. Three ruminal fistulated dry dairy cows were used for rumen fluid and fifteen conventional forages used in dairy cattle were collected around this island. The degradability of these feedstuffs with and without AFE ($Amaferm^{(R)}$.) treatment was measured using the $Daisy^{(R)}$. in vitro method. The roughage energy values, including TDN and NEL, were calculated according to Robinson (2000). Results from the 30 h in vitro neutral detergent fiber (NDF) degradability and predicted energy evaluations showed that alfalfa (among the forages) contained the highest degradability and energy values, Bermuda straw having the lowest. Peanut vines and corn silage contained higher energy values and the lowest value found in Pangola and Napier grasses among the locally produced forages. Pangola and Napier grasses had lower values than most imported forages except Bermuda straw. Among the by-products, wheat middling contained the highest NDF degradability, while rice bran contained the richest energy value due to its high oil content. From the dynamic dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) degradation, corn silage contained the highest effective degradation among the local forages; wheat middling (among the by-products) degraded the fastest in DM, OM, ADF and NDF and showed the highest effective degradability. AFE inclusion was inconsistent among the forages. Alfalfa hay showed significantly increased 30 h NDF degradability and energy values, Pangola hay, Napier grass and brewer's grains showed decreased degradability and energy values. AFE inclusion increased the DM, OM and NDF degradation rate in most forage, but only increased the DM degradation rate in sorghum distiller's grains, the OM degradation rate in bean curd pomace and the NDF and ADF degradation rates in soy pomace (among the by-products).

• 동아시아식생활학회지
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• 제24권1호
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• pp.28-41
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• 2014

식이섬유가 인체 내에서 나타내는 중요한 생리 기능은 각 구성 성분들의 함량과 특성에 의해 영향을 받으므로, 본 연구에서는 채소를 대상으로 식이섬유 각 성분들의 함량을 분별 측정하고, 열처리 시 변화를 조사하였다. 이상의 결과를 요약하면 다음과 같다. 각종 채소의 총 펙틴 함량은 건조물 기준으로 0.89~2.75 g/100 g의 범위였으며, 대부분 1~2 g/100 g 수준이었다. 펙틴의 각 분획 별 함량의 경우, 열수 가용성 펙틴 함량은 0.33~0.98 g/100 g, 인산 가용성 펙틴 함량은 0.29~0.81 g/100 g의 범위였고, 염산 가용성 펙틴 함량은 0.30~1.40 g/100 g의 범위로 세 가지 펙틴 분획 중에서 채소 종류에 따른 변이가 가장 컸다. 이상의 세 가지 펙틴 분획의 비율은 각 채소에서 대략 비슷한 수준이었다. 각종 채소의 불용성 식이섬유 함량은 건물 기준으로 총 불용성 식이섬유인 Neutral detergent fiber(NDF)는 11.8~31.9%, Acid detergent fiber(ADF)는 10.9~25.4%, cellulose는 8.8~23.8%, hemicellulose는 0.6~10.6%, lignin는 1.0~5.2% 범위였으며, 특히 고추류에서 총 불용성 식이섬유 함량이 높았다. Cellulose는 총 불용성 식이섬유인 NDF의 63% 정도를 차지하여 본 실험에 사용된 채소들의 경우, 불용성 식이섬유의 대부분이 cellulose로 구성되어 있었다. 가열 방법에 따른 펙틴 함량의 변화에 있어서, 총 펙틴 함량은 가열 처리 시 원료 시료에 비해 감소했으며, boiling시 가장 크게 감소하였다. 펙틴의 세 분획 중 열수 가용성 펙틴(HWSP) 함량은 증가했고, 비수용성 펙틴인 인산 가용성 펙틴(HXSP)과 염산 가용성 펙틴(HCLSP) 함량은 감소했다. 가열 처리에 의한 NDF, ADF, cellulose 함량의 변화는 원래 시료보다 증가했으며, 이들 성분들의 변화는 cellulose의 함량 변화에 기인했다. 그러나 hemicellulose와 lignin 함량은 일정한 경향을 보이지 않았다. 총 식이섬유 함량은 신선물 기준으로 1.20~7.11% 범위였으며, 마늘, 우엉, 고추잎 등에서 높았다. 총 식이섬유 함량의 대부분이 불용성 식이섬유로 구성되어 있음을 알 수 있었다. 총 식이섬유/조섬유 비율은 1.32~4.00으로 붉은 고추가 가장 낮고, 상치가 가장 높았으나, 채소 종류 간 큰 차이를 보이지 않았다.

• 한국식품영양과학회지
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• 제23권4호
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• pp.647-653
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• 1994

This study was conducted to investigate the contents of dietary fiber (DF), compositions of fatty acids in lipid fraction and amino acids in salt-soluble protein from the functional food source such as Platycodi radix, perilla seed, evening primrose seed and aloe vera. The contents of dietary fiber, neutral detergent fiber (N.D.F) , acid detergent fiber( A.D.F) . kignin, hemicellulose an dcellulose in evening primorse seed were higher than those of other samples, except the content of cellulose .The ration of polyunsaturated /saturated (P/S) fatty acid in total lipids was 6.31 in perilla seed, which was higher than those of other samples. The content of linolenic acid (n-3) in perilla seed was 55.47%. The content of linoleic acid (n-6) in evening primrose seed was 71.88% , which was higher than those of other samples. The fatty acid composition in neutral lipids were the same as those of total lipids. The PUFA contents of fatty acid in glycolipids were 61.76% in perilla seed. And also, the ratio of n-6/n-3 in evening primrose seed was 15.19. The fatty acid compositions in phospholipids were the sameas those of glycolipids. The contents of PUFA in Platycodi radix were 62.96% . The essential amino acid contents of salt-soluble protein were 47 mole % in Platycodi radix , which was slightly higher than those other samples. The ration of essential amino acid /nonessential amino acid (E/N) was 0.9 and 0.66 in Platycodi radix and aloe vera, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제17권7호
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• pp.967-972
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• 2004

Four Holstein$\times$Indigenous cows with ruminal canulas were used in a 4$\times$4 Latin square design with 28 d periods to determine the effect of replacing nonforage fiber source (NFFS) from cassava residues for non-fiber carbohydrates (NFC) on ruminal fermentation characteristics and milk production. Dietary treatments contained 17% forage neutral detergent fiber (FNDF) from corn silage and 0, 3, 6 and 9% nonforage NDF from cassava residues and 11% nonforage NDF from other NFFS, so that levels of nonforage NDF were 11, 14, 17 and 20% dry matter (DM). Intakes of DM and net energy for lactation, average daily gain and milk fat percentage were not different (p>0.05). Ruminal pH, ammonia concentrations, acetate to propionate ratios, 24 h in sacco fiber digestibility significantly increased with increasing contents of nonforage NDF from cassava residues. Concentrations of VFA, urinary excretion of purine derivatives, milk protein percentage, production of milk and 4% FCM significantly decreased. These results suggest that NFC in diets is one of the limiting factors affecting productivity of dairy cows in the tropics and thus NFFS is better used as partial replacements for FNDF.

• 한국초지조사료학회지
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• 제29권1호
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• pp.31-36
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• 2009

본 연구는 청예용 사초의 질을 신속하고 정확하게 측정할 수 있는 대안을 모색하기 위하여수행하였다. 근적외선분광분석법을 이용한 사초의 분석가능성을 타진하기 위해 2002년부터 2007년에 거쳐 생산된 각종 사초 258점을 시료로 사용하였다. 즉 시료는 조단백질, 조섬유, NDF, ADF 그리고 건물소화율을 분석하였으며, 또한 NIR System으로 $400{\sim}2,400nm$ 사이의 파장을 얻었다. 그리고 파장과 습식분석치를 이용하여 중회귀식을 만들고 이것을 사용하여 미지의 시료를 분석할 수 있는가를 검증하였다. 근적외선 분석법의 중요한 지표인 결정계수 $r^2$와 표준오차이며 본 실험의 결과 검증식의 $r^2$는 CP(crude protein), CF(crude fiber), ADF (acid detergent fiber), NDF (neutral detergent fiber) 그리고 IVTD(in vitro true digestibility)에서 각 각 0.70, 0.86, 0.94, 0.94 그리고 0.89였다. 검량식은 그 값이 0.47, 0.39, 0.89, 0.90 그리고 0.61이었다. 본 실험 결과 근적외선분광법에 의한 청예사료 질평가가 가능하며 특히 조섬유, ADF 그리고 진정인비트로 소화율 (IVTD)에서 유효할 것으로 나타났다. 그러나 보다 정확한 결과를 얻기 위해서는 시료는 한국사초의 대표성을 갖는 것을 수집하여 사용해야 할 것이다. 앞으로 더 많은 시료를 추가하면 모든 사초를 분석할 강고한 검량식이 작성될 수 있을 것으로 기대된다.

• 한국초지조사료학회지
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• 제20권3호
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• pp.155-160
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• 2000

This syudy was conducted to increase the utility of forage rye which are suitable to be cultivated at high altitude Taekwllyong area. For that purpose two cultivars of rye (winter-more, kool-glazer) were cultivated for 3years using a randomized block design with 3 replications. The dry matter digestibility (DMD) was evaluated with the pelleted rye hay produced by adding sodium hydroxide and then the TDN value was calculated by the DMD. By adding NaOH, DMD and TDN value of rye pellets (Y=46.92+ 1.8X, ~ 0 . 9 7 )w ere increased significantly (p<0.05) and NDF (neutral detergent fiber) decrease significantly. However, ADF (acid detergent fiber) and lignin did not significantly decrease (p<0.05). Rye could be cultivated as a second crop after com at high altitude area in the same year and pelleted with NaOH in order to increase the nutritive values (p< 0.05). (Key words : Dry matter digestibility (DMD), Forage rye, NDF, ADF, Sodium hydroxide)