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Effects of fermentation on protein profile of coffee by-products and its relationship with internal protein structure measured by vibrational spectroscopy

  • Samadi;Xin Feng;Luciana Prates;Siti Wajizah;Zulfahrizal;Agus Arip Munawar;Peiqiang Yu
    • Animal Bioscience
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    • v.36 no.8
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    • pp.1190-1198
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    • 2023
  • Objective: To our knowledge, there are few studies on the correlation between internal structure of fermented products and nutrient delivery from by-products from coffee processing in the ruminant system. The objective of this project was to use advanced mid-infrared vibrational spectroscopic technique (ATR-FT/IR) to reveal interactive correlation between protein internal structure and ruminant-relevant protein and energy metabolic profiles of by-products from coffee processing affected by added-microorganism fermentation duration. Methods: The by-products from coffee processing were fermented using commercial fermentation product, called Saus Burger Pakan, consisting of various microorganisms: cellulolytic, lactic acid, amylolytic, proteolytic, and xylanolytic microbes, for 0, 7, 14, 21, and 28 days. Protein chemical profiles, Cornell Net Carbohydrate and Protein System crude protein and CHO subfractions, and ruminal degradation and intestinal digestion of protein were evaluated. The attenuated total reflectance-Ft/IR (ATR-FTIR) spectroscopy was used to study protein structural features of spectra that were affected by added microorganism fermentation duration. The molecular spectral analyses were carried using OMNIC software. Molecular spectral analysis parameters in fermented and non-fermented by-products from coffee processing included: Amide I area (AIA), Amide II (AIIA) area, Amide I heigh (AIH), Amide II height (AIIH), α-helix height (αH), β-sheet height (βH), AIA to AIIA ratio, AIH to AIIH ratio, and αH to βH ratio. The relationship between protein structure spectral profiles of by-products from coffee processing and protein related metabolic features in ruminant were also investigated. Results: Fermentation decreased rumen degradable protein and increased rumen undegradable protein of by-products from coffee processing (p<0.05), indicating more protein entering from rumen to the small intestine for animal use. The fermentation duration significantly impacted (p<0.05) protein structure spectral features. Fermentation tended to increase (p<0.10) AIA and AIH as well as β-sheet height which all are significantly related to the protein level. Conclusion: Protein structure spectral profiles of by-product form coffee processing could be utilized as potential evaluators to estimate protein related chemical profile and protein metabolic characteristics in ruminant system.

Analysis of activated colloidal crud in advanced and modular reactor under pump coastdown with kinetic corrosion

  • Khurram Mehboob;Yahya A. Al-Zahrani
    • Nuclear Engineering and Technology
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    • v.54 no.12
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    • pp.4571-4584
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    • 2022
  • The analysis of rapid flow transients in Reactor Coolant Pumps (RCP) is essential for a reactor safety study. An accurate and precise analysis of the RCP coastdown is necessary for the reactor design. The coastdown of RCP affects the coolant temperature and the colloidal crud in the primary coolant. A realistic and kinetic model has been used to investigate the behavior of activated colloidal crud in the primary coolant and steam generator that solves the pump speed analytically. The analytic solution of the non-dimensional flow rate has been determined by the energy ratio β. The kinetic energy of the coolant fluid and the kinetic energy stored in the rotating parts of a pump are two essential parameters in the form of β. Under normal operation, the pump's speed and moment of inertia are constant. However, in a coastdown situation, kinetic damping in the interval has been implemented. A dynamic model ACCP-SMART has been developed for System Integrated Modular and Advanced Reactor (SMART) to investigate the corrosion due to activated colloidal crud. The Fickian diffusion model has been implemented as the reference corrosion model for the constituent component of the primary loop of the SMART reactor. The activated colloidal crud activity in the primary coolant and steam generator of the SMART reactor has been studied for different equilibrium corrosion rates, linear increase in corrosion rate, and dynamic RCP coastdown situation energy ratio b. The coolant specific activity of SMART reactor equilibrium corrosion (4.0 mg s-1) has been found 9.63×10-3 µCi cm-3, 3.53×10-3 µC cm-3, 2.39×10-2 µC cm-3, 8.10×10-3 µC cm-3, 6.77× 10-3 µC cm-3, 4.95×10-4 µC cm-3, 1.19×10-3 µC cm-3, and 7.87×10-4 µC cm-3 for 24Na, 54Mn, 56Mn, 59Fe, 58Co, 60Co, 99Mo, and 51Cr which are 14.95%, 5.48%, 37.08%, 12.57%, 10.51%, 0.77%, 18.50%, and 0.12% respectively. For linear and exponential coastdown with a constant corrosion rate, the total coolant and steam generator activity approaches a higher saturation value than the normal values. The coolant and steam generator activity changes considerably with kinetic corrosion rate, equilibrium corrosion, growth of corrosion rate (ΔC/Δt), and RCP coastdown situations. The effect of the RCP coastdown on the specific activity of the steam generators is smeared by linearly rising corrosion rates, equilibrium corrosion, and rapid coasting down of the RCP. However, the time taken to reach the saturation activity is also influenced by the slope of corrosion rate, coastdown situation, equilibrium corrosion rate, and energy ratio β.

Experimental assessment of thermal radiation effects on containment atmospheres with varying steam content

  • R. Kapulla;S. Paranjape;U. Doll;E. Kirkby;D. Paladino
    • Nuclear Engineering and Technology
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    • v.54 no.11
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    • pp.4348-4358
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    • 2022
  • The thermal-hydraulics phenomena in a containment during an accident will necessarily include radiative heat transfer (i) within the gas mixture due to the high radiative absorption and emission of steam and (ii) between the gas mixture and the surrounding structures. The analysis of some previous PANDA experiments (PSI, Switzerland) demonstrated the importance of the proper modelling of radiation for the benefit of numerical simulations. These results together with dedicated scoping calculations conducted for the present experiments indicated that the radiative heat transfer is considerable, even for a very low amount of steam (≈2%). The H2P2 series conducted in the large-scale PANDA facility at the Paul-Scherrer-Institut (PSI) in the framework of the OECD/NEA HYMERES-2 project is intended to enhance the understanding of thermal radiation phenomena and to provide a benchmark for corresponding numerical simulations. Thus, the test matrix was tailored around the two opposite extremes: either gas compositions with small steam content such that radiative heat transfer phenomena can be neglected. Or gas mixtures containing larger amounts of steam, so that radiative heat transfer is expected to play a dominant role. The H2P2 series consists of 5 experiments designed to isolate the radiation phenomena from convective and diffusive effects as much as possible. One vessel with a diameter of 4 m and a height of 8 m was preconditioned with different mixtures of air / steam at room and elevated temperatures. This was followed by the build-up of a stable helium stratification at constant pressure in the upper part of the vessel. After that, helium was injected from the top into the vessel which leads to an increase of the vessel pressure and a corresponding elevation-dependent and transient rise of the gas temperature. It is shown that even the addition of small amounts of steam in the initial gas atmosphere considerably impacts the radiative heat transport throughout all phases of the experiments and markedly influences i) the monitored gas peak temperature, ii) the temperature history during the compression and iii) the following relaxation phase after the compression was stopped. These PANDA experiments are the first of its kind conducted in a large scale thermal-hydraulic facility.

Radiation stability and radiolysis mechanism of hydroxyurea in HNO3 solution: Alpha, beta, and gamma irradiations

  • Yilin Qin;Wei Liao;Tu Lan;Fengzhen Li;Feize Li;Jijun Yang;Jiali Liao;Yuanyou Yang;Ning Liu
    • Nuclear Engineering and Technology
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    • v.54 no.12
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    • pp.4660-4670
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    • 2022
  • Hydroxyurea (HU) is a novel salt-free reductant used potentially for the separation of U/Pu in the advanced PUREX process. In this work, the radiation stability of HU were systematically investigated in solution by examining the effects of the type of rays (α, β, and γ irradiations), the absorbed dose (10-50 kGy), and the HNO3 concentration (0-3 mol L-1). The influence degree on HU radiolysis rates followed the order of the absorbed dose > the ray type > the HNO3 concentration, but the latter two had moderate effects on HU radiolysis products where NH4+ and NO2- were found to be the most abundant ones, suggesting that the differences of α, β, and γ rays should be considered in the study of irradiation effects. The radiolysis mechanism was explored using density functional theory (DFT) calculations, and it proposed the dominant radiolysis paths of HU, indicating that the radiolysis of HU was mainly a free radical reaction among ·H, eaq-, H2O, intermediates, and the radiolytic free radical fragments of HU. The results reported here provide valuable insights into the mechanistic understanding of HU radiolysis under α, β, and γ irradiations and reliable data support for the application of HU in the reprocessing of spent fuel.

Detecting Weak Signals for Carbon Neutrality Technology using Text Mining of Web News (탄소중립 기술의 미래신호 탐색연구: 국내 뉴스 기사 텍스트데이터를 중심으로)

  • Jisong Jeong;Seungkook Roh
    • Journal of Industrial Convergence
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    • v.21 no.5
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    • pp.1-13
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    • 2023
  • Carbon neutrality is the concept of reducing greenhouse gases emitted by human activities and making actual emissions zero through removal of remaining gases. It is also called "Net-Zero" and "carbon zero". Korea has declared a "2050 Carbon Neutrality policy" to cope with the climate change crisis. Various carbon reduction legislative processes are underway. Since carbon neutrality requires changes in industrial technology, it is important to prepare a system for carbon zero. This paper aims to understand the status and trends of global carbon neutrality technology. Therefore, ROK's web platform "www.naver.com." was selected as the data collection scope. Korean online articles related to carbon neutrality were collected. Carbon neutrality technology trends were analyzed by future signal methodology and Word2Vec algorithm which is a neural network deep learning technology. As a result, technology advancement in the steel and petrochemical sectors, which are carbon over-release industries, was required. Investment feasibility in the electric vehicle sector and technology advancement were on the rise. It seems that the government's support for carbon neutrality and the creation of global technology infrastructure should be supported. In addition, it is urgent to cultivate human resources, and possible to confirm the need to prepare support policies for carbon neutrality.

A cosmic ray muons tomography system with triangular bar plastic scintillator detectors and improved 3D image reconstruction algorithm: A simulation study

  • Yanwei Zhao;Xujia Luo;Kemian Qin;Guorui Liu;Daiyuan Chen;R.S. Augusto;Weixiong Zhang;Xiaogang Luo;Chunxian Liu;Juntao Liu;Zhiyi Liu
    • Nuclear Engineering and Technology
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    • v.55 no.2
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    • pp.681-689
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    • 2023
  • Purpose: Muons are characterized by a strong penetrating ability and can travel through thousands of meters of rock, making them ideal to image large volumes and substances typically impenetrable to, for example, electrons and photons. The feasibility of 3D image reconstruction and material identification based on a cosmic ray muons tomography (MT) system with triangular bar plastic scintillator detectors has been verified in this paper. Our prototype shows potential application value and the authors wish to apply this prototype system to 3D imaging. In addition, an MT experiment with the same detector system is also in progress. Methods: A simulation based on GEANT4 was developed to study cosmic ray muons' physical processes and motion trails. The yield and transportation of optical photons scintillated in each triangular bar of the detector system were reproduced. An image reconstruction algorithm and correction method based on muon scattering, which differs from the conventional PoCA algorithm, has been developed based on simulation data and verified by experimental data. Results: According to the simulation result, the detector system's position resolution is below 1 ~ mm in simulation and 2 mm in the experiment. A relatively legible 3D image of lead bricks in size of 20 cm × 5 cm × 10 cm used our inversion algorithm can be presented below 1× 104 effective events, which takes 16 h of acquisition time experimentally. Conclusion: The proposed method is a potential candidate to monitor the cosmic ray MT accurately. Monte Carlo simulations have been performed to discuss the application of the detector and the simulation results have indicated that the detector can be used in cosmic ray MT. The cosmic ray MT experiment is currently underway. Furthermore, the proposal also has the potential to scan the earth, buildings, and other structures of interest including for instance computerized imaging in an archaeological framework.

Prediction of the remaining time and time interval of pebbles in pebble bed HTGRs aided by CNN via DEM datasets

  • Mengqi Wu;Xu Liu;Nan Gui;Xingtuan Yang;Jiyuan Tu;Shengyao Jiang;Qian Zhao
    • Nuclear Engineering and Technology
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    • v.55 no.1
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    • pp.339-352
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    • 2023
  • Prediction of the time-related traits of pebble flow inside pebble-bed HTGRs is of great significance for reactor operation and design. In this work, an image-driven approach with the aid of a convolutional neural network (CNN) is proposed to predict the remaining time of initially loaded pebbles and the time interval of paired flow images of the pebble bed. Two types of strategies are put forward: one is adding FC layers to the classic classification CNN models and using regression training, and the other is CNN-based deep expectation (DEX) by regarding the time prediction as a deep classification task followed by softmax expected value refinements. The current dataset is obtained from the discrete element method (DEM) simulations. Results show that the CNN-aided models generally make satisfactory predictions on the remaining time with the determination coefficient larger than 0.99. Among these models, the VGG19+DEX performs the best and its CumScore (proportion of test set with prediction error within 0.5s) can reach 0.939. Besides, the remaining time of additional test sets and new cases can also be well predicted, indicating good generalization ability of the model. In the task of predicting the time interval of image pairs, the VGG19+DEX model has also generated satisfactory results. Particularly, the trained model, with promising generalization ability, has demonstrated great potential in accurately and instantaneously predicting the traits of interest, without the need for additional computational intensive DEM simulations. Nevertheless, the issues of data diversity and model optimization need to be improved to achieve the full potential of the CNN-aided prediction tool.

Evaluation on Adiabatic Property for Vehicular Sandwich Composite Structure (차체 구조용 샌드위치 복합소재 단열 특성 평가)

  • Lee Sang Jin;Oh Kyung Won;Jeong Jong Cheol;Kong Chang duk;Kim Jeong Seok;Cho Se Hyun
    • Composites Research
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    • v.19 no.1
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    • pp.9-14
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    • 2006
  • Experimental investigation on heat transfer ratio was firstly performed with three types of sandwich panels such as the Carbon/Epoxy Skin-Aluminum Honeycomb and Balsa Core Sandwich Panel of 37mm thickness, the Carbon/Epoxy Aluminum Skin-Honeycomb Core Sandwich Panel of 57mm thickness (including insulator) and the Carbon/Epoxy Skin-Aluminum Honeycomb Core Sandwich Panel of 37mm thickness based on the KS F 2278:2003(Insulation test method of windows). In additional to this investigation, experimental tests were also done for evaluation of heat transportation ratio with the Aluminum Skin- Aluminium Honeycomb Sandwich Panels of 27mm and 35mm thickness, and Aluminum Skin-Foaming Aluminum Sandwich Panel of 27mm thickness by the KS F2277:2002 (Insulation measuring method of construction component-Calibration heat box method or protective heat box method). In this study, it was found that the larger net heat transfer cross sectional area between the skin and the sandwich core is given, the higher heat transportation ratio occurs. It was also found that the hybrid type insulation had better insulation characteristics compared to the non-hybrid type insulation.

A Study on the Success Model for the Establishment of Big Data System in Public Institutions (공공기관 빅데이터 시스템 구축을 위한 성공모형에 관한 연구)

  • Lee, Gwang-Su;Kwon, Jungin
    • Journal of Digital Convergence
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    • v.20 no.1
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    • pp.129-139
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    • 2022
  • This study aims to identify which factors affect successful big data system construction, identify the relationship between the factors, and identify the success model and success factors necessary for public institutions to build big data systems. Therefore, the preceding and related studies related to this study were reviewed, and success factors for the establishment of a big data system were derived based on this. As a research method, a survey was conducted on users of institutions that have established or planned to build a big data system, and a structural equation (AMOS) was conducted to verify the impact relationship between success factors. As a result of the analysis, organizational support factors, development support factors, user support factors, information quality, service quality, system quality, use, and net benefit were derived as success factors for building big data systems, and a success model was presented. This can be seen as significant and academic contributions in that it is the first study of the success model for building an information system reflecting big data characteristics, and it is expected that this study will be used as basic data for building a big data system in public institutions in the future.

A Study on Improving Strategies of Itinerant Librarians' Job Environment at Small Libraries (작은도서관 순회사서 직무환경 개선방안 연구)

  • Chae, Hee-ra;Kim, You-seung
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.33 no.1
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    • pp.71-94
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    • 2022
  • While small libraries have steadily increased in quantity, parts of qualitative growth such as lack of manpower and poor operation have been pointed out as problems. Accordingly, with the aim of establishing a foundation for linkage and cooperation between public libraries and small libraries, the small library itinerant librarians project has been promoted since 2010, and its scale has been continuously expanding. However, the lack of manpower and poor operation still remain tasks to be solved, and the poor treatment and job environment of itinerant librarians have not improved. In this context, this study aims to examine the problems of small libraries and job problems experienced by itinerant librarians and to find ways to improve the job environment of itinerant librarians based on this. Through the analysis of previous studies, small libraries and net companies were investigated, and in-depth interviews were conducted with participants related to itinerant librarians and small libraries. Through interview data analysis, a total of 17 meaning topics were extracted, including 'lack of work authority', 'lack of work manual', 'lack of communication, community', 'lack of integrated operating system', 'poor treatment and working environment', 'malicious user', and 'lack of belonging'. Based on this, four improvement measures were proposed, including the improvement of the job authority and standard regulations of itinerant librarians, the establishment of a community organization, job security guarantees, and job education reinforcement.