• Korean Journal of Plant Taxonomy
• /
• v.39 no.3
• /
• pp.229-232
• /
• 2009

Nervilia nipponica Makino (Orchidaceae), a genus and species previously unrecorded for the Korean flora, was collected in the deciduous forests of Mt. Halla, Jeju Island. This species was known to be distributed mainly from tropical to subtropical regions of Taiwan and Japan. Genus Nervilia is distinguished from other genera of tribe Nervilieae (Orchidaceae) by having only a single, plicate leaf which develops and senesces before flowering shoots appear, stems unbranched, two pollinia, and sometimes lacking a viscidium.

• Journal of Plant Biotechnology
• /
• v.50
• /
• pp.108-114
• /
• 2023

In vitro conservation is one of the most effective strategies for rare plant protection, especially for orchid species. To maximize the success rates of in vitro explant establishment (stage I) in conservation programs, the application of tissue culture additives such as Plant Preservative Mixture^TM (PPM^TM) should be emphasized. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model for the evaluation of PPM^TM's phytotoxicity in the meristematic tissues of epiphytic orchids. PPM^TM had no observable inhibitory effect on protocorm, shoot, or root development when it was supplemented at 0.1%. PPM^TM supplementation caused adverse effects on D. thyrsiflorum explants at concentrations > 0.2%. At high concentrations, young in vitro seedlings showed damage, especially at the root tissue level. Based on this model, supplementation of 0.1-0.2% PPM^TM to culture media was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.