• 한방비만학회지
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• 제17권2호
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• pp.96-100
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• 2017

Objectives: Samjung-hwan (SJH) is a Korean herbal formula that has been used for antilipidemic, antioxidant, and antidepressant effects. As a part of safety evaluation, single oral dose toxicity study of modified SJH in Sprague-Dawley (SD) rats was carried out. Methods: Modified SJH extract was administered orally in male and female SD rats at dose of 5,000 mg/kg. After single oral administration of modified SJH, we monitored the changes of mortalities, clinical signs, and body weight daily for 14 days. Gross necropsy findings were also evaluated at the end of the experiment. Results: Modified SJH extract did not induce any toxic signs in the changes of mortalities, clinical signs, and body weight. There were also observed no gross necropsy findings in rats. Conclusions: As results, the approximate lethal dose of modified SJH extract in SD rats was considered to be over 5,000 mg/kg. This suggests that modified SJH is safe herbal formula for the treatment with.

• 한방비만학회지
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• 제18권1호
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• pp.36-49
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• 2018

Objectives: The study is aimed at evaluating the possible toxicity in 90-day repeated oral administration of modified Samjung-hwan (mSJH) in Sprague-Dawley (SD) rats. This study was conducted to detect the no-observed adverse effect level (NOAEL). Methods: Modified SJH extract was administered orally in male and female SD rats at dose of 0, 1,000, 2,000, 4,000 mg/kg. Each group consisted of 10 rats of each gender. The modified SJH extract was given once a day for 90 days. We monitored the changes of mortalities, clinical signs, body weight changes, food consumption, ophthalmologic findings, urine analysis, hematology, serum biochemistry, necropsy findings, organ weights, histological markers of all animals treated with modified SJH extract during the study period. Results: There were no toxicologically significant changes in mortalities, clinical signs, body weight gains, food consumption, ophthalmologic findings, urine analysis, hematology, serum biochemistry, necropsy findings, organ weights, histological markers in any of rats tested. Conclusions: The NOAEL of the modified SJH extract in male rats and no observed effect level (NOEL) in female rats are considered 4,000 mg/kg.

• 대한약침학회지
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• 제26권1호
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• pp.86-93
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• 2023

Objectives: This study aimed to evaluate the potential toxicity of a recently developed and clinically used No-Pain pharmacopuncture (NPP) solution. We also assessed the lethal dose of the NPP agent following a single intramuscular injection in Sprague-Dawley (SD) rats. Methods: Animals were divided into two groups: the NPP test material group and the normal saline control group. A single intramuscular injection of the NPP agent (1.0 mL/animal) was administered to rats of the NPP test material group. The control group rats received the same volume of normal saline. Both female and male rats were included in each group. All rats were monitored for clinical signs and body weight changes for 14 days after administration of the test substance or saline. At the end of the observation period, a gross necropsy was conducted and localized tolerance at the injection site was analyzed. Results: No mortality was observed in the NPP test material and control groups. Moreover, no test substance-related effects were observed on clinical signs, body weight, necropsy findings, and localized tolerance at the injection site. Conclusion: The approximate lethal dose of the NPP agent is greater than 1.0 mL/animal under the conditions used in this study. Additional toxicity evaluations and clinical studies are needed to confirm the safety of NPP use in clinical practice.

• 한국환경생태학회지
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• 제34권4호
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• pp.282-293
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• 2020

본 연구에서는 2009-2019년 기간 동안 대전시 외곽의 도심개발에 따른 인근 하천(관평천)의 상류, 중류, 하류의 구간에서 4회 조사(2009, 2010, 2016, 2019)를 통해 이화학적 수질, 어류 종 조성, 생태 건강성 특성에 대해 장기간 변화추이를 분석하였다. 본 하천지역에서 도심개발은 2008년에 이루어졌고, 2012년에는 하천복원사업이 수행되어 하천복원 전과 후의 특성변화 추이를 모니터링 하였다. 하천 생태건강도 평가를 위해 군집수준의 어류평가지수(Fish Assessment Index, FAI)를 이용하였고, 어류의 기관(Organ) 수준에서 해부학적 건강도(Necropsy-based Health Assessment Index, HAI) 분석을 실시하였다. 이화학적 수질 분석에서는 중류(St. 2)에서 가장 낮은 탁도와 엽록소(Chl-a)가 측정되었다. 이는 빠른 유속에 의한 물리적 요인 때문으로 나타났다. 어류 조사에서는 총 18종이 채집되었고, 피라미(Zacco platypus)가 가장 우점 하는 종(40.6%)으로 나타났다. 하천복원 직후(2016)에는 민감종(Sensitive species)과 충식종(Insectivore species)이 우점, 종 다양도 및 종 풍부도 지수 상승, 생태건강도 지수(FAI)가 상승하여 생태 건강도는 "최상상태(A: 87.5)"로 나타났으나 가장 최근 조사인 2019년에는 전 기간에 비해 악화되는 것으로 나타났다. 기관(Organ) 분석에 의거한 해부학적 건강도 지수(HAI) 분석에 따르면, 피부손상(Skin)은 상류에서, 신장 손상(Kidney)은 하류 역에서 나타났고, 간(Liver)과 아가미(Gill)의 손상은 모든 지점에서 나타나 해부학적 측면의 건강도에서도 영향을 받는 것으로 나타났다.

• 대한약침학회지
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• 제17권3호
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• pp.31-39
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• 2014

Objectives: This study was performed to analyze the toxicity and to find the lethal dose of the test substance Water-soluble Carthami-flos pharmacopuncture (WCF) when used as a single intravenous-dose in 6-week-old, male and female Sprague-Dawley rats. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices. 20 female and 20 male Spague-Dawley rats were divided into 4 groups of 5 female and 5 male animals per group. The rats in the three experimental groups received single intravenous injections with 0.125-mL, 0.25-mL and 0.5-mL/animal doses of WCF, Groups 2, 3, and 4, respectively, and the control group, Group 1, received a single intravenous injection with a 0.5-mL dose of normal saline. Clinical signs were observed and body weight measurements were carried out for 14 days following the injections. At the end of the observation period, hematology, clinical chemistry, histopathological tests and necropsy were performed on the injected parts. Results: No deaths occurred in any of the groups. Also, no significant changes in body weight, hematological parameters or clinical chemistry test results between the control group and the experimental groups were observed. Visual inspection after necropsy showed no abnormalities. Histopathological tests on the injected parts showed no significant differences, except for Group 1 females; however, the result was spontaneous generation and had no toxicological meaning because it was not dose-dependent. Therefore, this study showed that WCF had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. Conclusion: As a result of single intravenous-dose tests of the test substance WCF in 4 groups of rats, the lethal dose for both males and females exceeded 0.5 mL/animal. Therefore, WCF is a relatively safe pharmacopuncture that can be used for treatment, but further studies should be performed.

• 한국임상수의학회지
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• 제17권1호
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• pp.173-177
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• 2000

A 3-year-old male Tosa was presented the severe dyspnea, emaciation and dehy dration. By echocardiograpy, right ventricle was found to be a mobile mass dynamically occluding the right ostium atrioventriculare in the systolic phase. At necropsy, 14 days after ultrasonography multiple tumor masses of various size were observed in the heart base, right ventricular lumen, myocardium, lung and liver. Histopathologically, the tumor cells, arranged in sheets or nests, were diagnosed as metastatic intracavitary cardiac aortic body tumor

• 대한수의사회지
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• 제36권3호
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• pp.218-221
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• 2000

Five farmed guinea fowl(Numidia meleagris) ared 6months in S farm of Kunsan, were submitted to Iksan branch, Livestock Development and Research Institute of Chonbuk province for necropsy. Grossly, systematic anemia, depressed, emaciation, and mild blood d

• Environmental Analysis Health and Toxicology
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• 제11권3_4호
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• pp.1-10
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• 1996

The purpose of this study was to characterise the subacute toxic potency of i.v. administered KHPC-005 and 006. Few test compounds-related toxic effects were observed in body weight gain, clinical signs, urinalysis, hematological parameters and serum biochemical values. Gross necropsy and histopathology revealed no evidance specific toxicity. Our data indicated that no-observed effect level of KI-IPC-005 and 006 were estimated to be 10mg/kg and 4mg/kg in male rats, and 10mg/kg and 1.33mg/kg in female rats, respectively.

• 한국임상수의학회지
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• 제2권1호
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• pp.155-159
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• 1985

The lesions of maxillary swelling observed during necropsy of a korean native pony aged 5 years old were consistent with osteodystrophia fibrosa microscopically. The maxillary lesion was characterized by completely altered bone structure showing complex processes of marked bone resorption with extensive fibrous replacement and accelerated osteoid formation.

• 한국임상수의학회지
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• 제19권2호
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• pp.247-249
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• 2002

A 6-month-old female Pekingese was euthanized due to poor progrosis after 1 month history of neurologic signs that include depression, ataxia, urination and defecation difficulty. At necropsy, no significant gross abnormalities were noted Histologically, neuronal vacuolation was noted in the brain, primarily cerebellum and occasionally in the brain stem area. Neuronal necrosis and secondary axonal swelling were also observed. Differential diagnoses were able to rule out other diseases which can induce neuronal vacuolation such as lysosomal storage disease, prion infection, and postvaccinal change.