• 한국발생생물학회지:발생과생식
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• 제18권2호
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• pp.117-125
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• 2014

Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than that of the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome systems of mature oocyte. To get the best way for keeping competence of matured oocytes, we studied the best conditions for vitrification focused on equilibration times. The mature oocytes were underwent vitrification with current popular method and analyzed the survival rates, microtubule stability and DNA integrity. The survival rates of recovered oocyte are almost same between groups and are more than 93%. The structural configuration of meiotic spindle was well kept in 10 min equilibration group and the stability rate was almost same with that of control. The chromosomal breakdown was observed in all experimental groups, but the chromosomal stability was higher in 10 min equilibration group than the other groups. The 10 min equilibration group showed best condition compared with the other groups. Based on these results, the equilibration time is one of the key factors in successful keeping for competence of mature oocyte. Although, more fine analysis about the effects of physical stress on oocyte during vitrification is needed to define the optimal condition, it is suggested that the optimal equilibration time to get competent oocyte in mouse is 10 min. Information acquired this study may provide insight into intracellular structural events occurring in human oocytes after vitrification and application for cryopreservation of human oocyte.

• 한국발생생물학회지:발생과생식
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• 제16권3호
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• pp.219-226
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• 2012

It is suggested that carbohydrate metabolites may involve in the development of morula to blastocyst but many of the mechanisms are not unmasked. Two-cell stage embryos were collected and examined the effects of lactate on the development of blastocyst in vitro. The expression profiles of lactate dehydrognase (Ldh) genes and aquaporin (Aqp) genes were analyzed with RT-PCR. The successful development from morula to blastocyst was dependent on lactate concentrations. The expression profiles of Ldh genes were changed by the lactate concentration. Ldha was expressed in morula stage at 10 mM lactate, and in blastocyst stage at lactate free condition. Ldhb was expressed in morula stage at 10 mM and 20 mM lactate, and in blastocyst stage at 10 mM lactate. Aqp genes were also showed different expression patterns by the lactate concentrations. Aqp3 was expressed in hatching embryo at 120 hr post hCG administration (hph) which was cultured in BWW medium and lactate free condition. Aqp7 was expressed in hatching embryos at 120 hph which was cultured at 10 mM lactate condition. Also Aqp8 was expressed in hatching embryo at BWW and 20 mM lactate condition. Aqp9 was expressed in morula at BWW and 10 mM lactate condition, and in blastocyst at BWW. Based on these results, it is suggested that concentration of lactate in the medium and the level of lactate synthesis in embryo is critical factor for blastocoels formation. In addition it is suggested that LDH may involve the AQPs expression in embryos.

• 한국발생생물학회지:발생과생식
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• 제19권1호
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• pp.11-17
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• 2015

This study investigated possible involvement of photoperiodic regulation in reproductive endocrine system of female olive flounder. To investigate the influence on brain-pituitary axis in endocrine system by regulating photoperiod, compared expression level of Kisspeptin and sbGnRH mRNA in brain and FSH-${\beta}$, LH-${\beta}$ and GH mRNA in pituitary before and after spawning. Photoperiod was treated natural photoperiod and long photoperiod (15L:9D) conditions from Aug. 2013 to Jun. 2014. Continuous long photoperiod treatment from Aug. (post-spawning phase) was inhibited gonadal development of female olive flounder. In natural photoperiod group, the Kiss2 expression level a significant declined in Mar. (spawning period). And also, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels were increasing at this period. However, in long photoperiod group, hypothalamic Kiss2, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels did not show any significant fluctuation. These results suggest that expression of hypothalamic Kiss2, GtH and GH in the pituitary would change in response to photoperiod and their possible involvement of photoperiodic regulation in reproductive endocrine system of the BPG axis.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.253-264
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• 2012

Nesfatin-1 is a recently discovered anorexigenic peptide which is distributed in several brain areas implicated in the feeding and metabolic regulation. Recently, it has been reported that nesfatin-1 is expressed not only in brain, but also in peripheral organs such as digestive organs, adipose tissues, heart, and reproductive organs. Nesfatin-1 is markedly expressed in the pancreas, stomach and duodenum. Eventually, the nesfatin-1 expression in the digestive organs may be regulated by nutritional status, which suggests a regulatory role of peripheral nesfatin-1 in energy homeostasis. Nesfatin-1 is also detected in the adipose tissues of humans and rodents, indicating that nesfatin-1 expression in the fat may regulate food intake independently, rather than relying on leptin. In addition, nesfatin-1 is expressed in the heart as a cardiac peptide. It suggests that nesfatin-1 may regulate cardiac function and encourage clinical potential in the presence of nutrition-dependent physio-pathologic cardiovascular diseases. Currently, only a few studies demonstrate that nesfatin-1 is expressed in the reproductive system. However, it is not clear yet what function of nesfatin-1 is in the reproductive organs. Here, we summarize the expression of nesfatin-1 and its roles in brain and peripheral organs and discuss the possible roles of nesfatin-1 expressed in reproductive organs, including testis, epididymis, ovary, and uterus. We come to the conclusion that nesfatin-1 as a local regulator in male and female reproductive organs may regulate the steroidogenesis in the testis and ovary and the physiological activity in epididymis and uterus.

• 한국강구조학회 논문집
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• 제24권6호
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• pp.701-710
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• 2012

최근에는 고층구조물이나 해양구조물(플랜트)의 경우 대부분 대형화로 설계 및 시공되고 있다. 대형구조물의 경우 주로 강재(steel)를 사용하여 설계하고 있다. 구조물 설계는 설계단계에서 최상의 부재를 선택하는 최적화를 적용함으로서 비용을 줄이는 경제적인 효과를 얻을 수 있다. 본 연구에서는 트러스와 프래임 구조물의 최적설계에 연속변수와 이산변수를 사용하여 최적화 하였고, 설계에서 사용된 이산자료는 규격화된 강재에 대한 자료와 데이터화된 이산자료를 사용하였다. 최적화는 이 모두에 적합한 이산최적화를 적용하였다. 최적화 기법으로는 유전자 알고리즘을 사용하였으며, 사용된 구조물은 10-Bar, 25-Bar 트러스와 1경간 2층, 1경간 7층 프레임 구조물이다. 설계시 제약조건은 고유진동수와 부재응력, 변위제약조건들이 적용되었다. 유전자 알고리즘에는 번식과장에서 엘리트 개체가 2번 사용되도록 하였으며, 제약조건 위반시 패널티를 사용하는 방법을 사용하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1453-1457
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• 2002

The experiment was conducted to find out the effect of 4 feeding systems on growth, feed intake, eating behavior, lactation performance, gestation period, post-partum heat period, conception rate and parasitic infestation of Black Bengal goat. Twenty four does of approximately 1 year of age were randomly selected for 4 treatment (feeding systems) groups having 6 replications in each. Treatments were stall feeding ($T_1$), tethering ($T_2$), restricted grazing ($T_3$) and grazing ($T_4$). $T_1$ group was housed continuously and adequate amounts of natural grass were supplied for ad libitum feeding. $T_2$ group was tethered for grazing natural grass from 8 a.m. to 4 p.m. being moved at one hour intervals. Goats of $T_3$ group were allowed grazing from 8 a.m. to 1 p.m. $T_4$ group was grazed from 8 a. m. to 4 p.m. Concentrate supplement was given at the rate of 150 g per day per goat for all of the treatment groups. Duration of experiment was 219 days. Daily live weight gain was significantly (p<0.05) higher in case of stall fed goats than that of others. DM intake also significantly (p<0.05) differed among the treatment groups and was 3.40 3.95, 3.76 and 4.05 per cent of their live weight for stall feeding, tethering, restricted grazing and grazing groups, respectively. Rate of rumination was significantly (p<0.05) higher in case of tethering group of goats than that of others. Birth weight of kids, milk yield, lactation period and post-partum heat period were significantly higher in case of stall fed goats than others. Tethering group showed significantly (p<0.05) higher litter size than others. Infestation rate of Fasciola was significantly (p<0.05) higher in the grazing group. In conclusion, it may be stated that among these feeding groups overall performance of stall fed goats was more satisfactory, and that the tethering group showed better performance than the others.

• 한국발생생물학회지:발생과생식
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• 제16권2호
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• pp.129-135
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• 2012

선행연구에서 본 연구자는 IEX-1 단백질이 난소 암세포에서 세포사멸(apoptosis)을 유도하는 기능을 수행함을 확인하였으나, 세포사멸과 세포생존의 여러 단계에서 어떠한 신호전달 체계로 IEX-1이 작용하는지는 정확히 알지 못하고 있다. 따라서 IEX-1 단백질과 결합하는 새로운 단백질을 찾기 위해 yeast two-hybrid system을 이용하였다. 그 결과 IEX-1이 여러 다양한 인간 암 세포에서 세포사멸을 유도하는 CATHEPSIN B 단백질과 결합함을 밝혔다. 본 연구에서는 리소좀 프로테아제의 일원인 CATHEPSIN B 단백질과 IEX-1 단백질의 결합을 면역침강법과 western blot 분석으로 확인하였다. 그러므로 이 결과들을 통해서 IEX-1과 CATHEPSIN B는 세포 내에서 서로의 기능에 영향을 미칠 것으로 예상되며, 세포사멸을 유도하는 IEX-1 단백질이 lysosome-mediated apoptotic pathway에 관여할 가능성을 시사한다.

• 한국어류학회지
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• 제28권2호
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• pp.67-71
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• 2016

시각세포는 많은 종에서 알려지지 않았지만 종의 서식환경과 매우 밀접한 연관성을 같는 것으로 알려져 있다. 이에 흰줄납줄개와 각시붕어 두 종의 시각세포를 광학현미경으로 조사하였다. 두 종의 눈은 비교적 큰 것으로 확인되었다. 신경연합층은 가장 바깥쪽부터 망막색소상피층, 시각세포층, 외핵층, 외망상층, 내핵층, 내망상층, 신경절세포층 등으로 구성된다. 특히 시각세포들이 존재하는 시각세포층을 확인한 결과, 매우 다양한 시각세포들을 보유하고 있었다. 긴 단일원추세포, 짧은 단일원추세포, 길고 짧은 원추세포가 쌍을 이룬 이형이중원추세포와 간상세포로 구성되어 있었다. 이들의 수평단면을 확인한 결과, 열 배열의 형태로 나타났으며 다양한 시각세포 종류와 열 배열을 갖는 특징은 이들의 서식환경과 밀접한 연관성이 있는 것으로 확인되었다.

• 복식
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• 제51권6호
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• pp.5-24
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• 2001

This paper is intended to explore femininity the ideal beauty of body and the features in fashion pursued in the Fashion system and the Anti-Fashion Movement in the Victorian period, on which the modern fashion is based. For the informative facts needed in this paper, books on history, fashion history, feminism, art history of aestheticism and the ideal beauty of body are referred to. On the part Ⅱ of this paper, the femininity and the ideal beauty of body implied in the Aesthetic Movement and Rational Dress Movement as the anti-fashion movement in the Victorian period will be reviewed. Following are the conclusion : First, the works in Aesthetic Movement mainly include the image of sensual female. The essence of femininity is categorized by cultural value, poetic spirit. appetite for sex and self-expression. The ideal beauty of body pursued in this movement is the beauty of immatured body, which means rejection of maternity as well as appetite for sex in the form of metaphor of the power and enthusiasm of female. The features of Aesthetic Movement emphasize the image of sensual and characteristic woman. These features are expressed in the natural waist line and the vertical H silhouette of high waist, natural exposure of body by means of drapery, simplicity and decency by design without fixed forms and seemingly faded colors. Second, Rational Dress Movement attempted to evaluate the femininity in a different way and ultimately pursued masculinity. Therefore, morality, liberty, intelligence, spirituality, self-control, willingness, which had been believed to exclusively belong to male, are added to the categories of femininity. The ideal beauty of body is expressed in the form of Venus Coelestic which is refined and strong. This symbolizes woman's freeing from the fate of reproduction and subordinate relationship with male, morality, decision of one's own and willingness. The features of Rational Dress Movement represent the image of strong-willed and moral woman in its internal meaning. The features of its fashions represent the concealment of the body, emphasis on activeness in pants without decoration and simplicity in its external form. All these features resulted from the pursuit of masculinity.

• Reproductive and Developmental Biology
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• 제34권4호
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• pp.275-281
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• 2010

The aim of this study was to investigate what protein(s) of porcine epididymal fluid (pEF) are able to enhance the nuclear maturation of porcine germinal vesicle (GV) oocytes in vitro. Proteins of pEF were fractionated by affinity, ion exchange, and gel filtration chromatography. Porcine cumulus-oocytes complexes (COC) from follicles were cultured in tissue culture medium (TCM 199) containing various fractions obtained by chromatography. Porcine COCs were also cultured in TCM 199 containing various meiosis inhibitors and pEF. After 24 or 48 h culture, oocytes were examined for evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II. When porcine COCs were cultured in the medium with meiosis inhibitor such as, dibutyryl cAMP (dbcAMP) and forskolin (Fo), more than 80% of oocytes were unable to resume meiosis. However, porcine COCs supplemented with pEF were able to overcome the inhibitory effect of dbcAMP and Fo. Maturation rate of oocytes was significantly (p<0.05) increased in the media supplemented with cationic protein(s) during in vitro maturation than in those with anionic protein(s) (44.1% vs 20.0%). When oocytes were cultured in the TCM 199 with fractions obtained by gel filtration, the maturation rate of oocytes was significantly (p<0.05) higher in fraction 11 containing 18 kDa than other fractions. The present study suggests that 1) dbcAMP and Fo prevent the spontaneous maturation of oocyte after isolation from follicles, and that pEF contain a substance(s) that improves meiosis resumption in vitro of porcine COCs, 2) cationic 18 kDa protein(s) are responsible for promotion of Mil stage.