• Development and Reproduction
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• 제10권3호
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• pp.197-202
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• 2006

Sex steroids are generally considered as natural sex inducers in fish, and aromatase (cytochrome P450 aromatase) that catalyzes androgens into estrogens in the steroidogenic pathway is also known to be involved in sex differentiation. The timing of aromatase action is, thus, of central importance in the study of fish sex differentiation. We treated sexually undifferentiated tilapia (Oreochromis niloticus) larvae with $Fadrozole^{TM}$, a non-steroidal aromatase inhibitor (AI), by immersing the fish in a solution containing AI during the sex differentiation period to narrow down the critical period of aromatase action. Fish were treated once at 11 or 13 days post fertilization (dpf), or twice at 11 and 13 dpf. The concentrations of AI at each time of the treatment were 0 mg/L (control), 50 mg/L or 100 mg/L. Survival rate was not statistically associated with AI immersion treatment (p>0.25). However, sex ratio was significantly altered by the treatment, with higher concentration and double immersion being more effective in masculinizing genetic females (p<0.05). These results suggest that aromatase action for sex differentiation in this fish species would begin at least from 11 dpf which is much earlier than previously expected, and that only 3 hours of brief immersion in AI solution is powerful enough to alter genetically programed sex.

• The Korean Journal of Mycology
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• 제18권4호
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• pp.203-208
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• 1990

Sexual reproductive structure of Phytophthora capsici in vitro was round shape with thick wall and $24.4\;{\mu}m$ of diameter ranging $20-32.5\;{\mu}m$. Oogonium was $26.7\;{\mu}m$ $(21-37.5\;{\mu}m$) and $6.5-{\times}5.4m$ $(6-8{\times}5-6{\mu}m)$ for antheridium as doughnut shaped. Since mycelial contact of the paired cultures initiated right after inoculation, mycelial expansion phase was followed. Oospore morphogensis could be divided into the four phases for reproducing adult oospores. The optimum temperature for oospore reproduction was $20-24^{\circ}C$, whereas a retard trend for oospore formation was at the temperature above $25^{\circ}C$. Korean squash agar medium showed a higher oospore formation than the existing V-8 agar medium. Red pepper fruit agar medium was next. No oospore was reproduced on the red pepper leaf medium. Diurnal light with $1800{\pm}300$ and $800{\pm}300$ Lux showed rather retardation for oospore formation than dark conditions.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제32권4호
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• pp.438-443
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• 1999

Germination and growth of Laminaria japonica microscopic stages were investigated under crossed gradients of temperatures and irradiances, and the results related to the seasonal temperature regime in the southeastern coast of Korea. Germination rates of $70\~86\%$ were observed in the temperature range of $5\~20^{\circ}C$, however, at $25^{\circ}C$ no germination of meiospores was observed. The primary factor affecting germination rates at the temperature range of $5\~20^{\circ}C$ was irradiance: germination was significantly reduced at $150 {\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. Vegetative cell production of female gametophytes was highest at $20^{\circ}C$, but plants were not fertile at the temperature. In the temperature range of $5\~15^{\circ}C$, higher irradiance caused females to reduce cell production, but increased fertility. Cell production was also low at lower temperatures with increased fertility rates. Optimal growth temperature for microsporophytes was $10^{\circ}C$ and their growth rates were light-saturated at $70 {\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. We have concluded that meiospores released before July could develope to the young sporophytic stage in the southeastern coast of Korea which is off the southern limit of its geographical distribution. However, limiting factor in the development of natural sporophytic population in this region would be the upper temperature limit for the survival of young sporophytes, as water temperature at this area frequently exceeds $25^{\circ}C$ during the summer period.

• Korean Journal of Breeding Science
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• 제40권1호
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• pp.84-87
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• 2008

Kokang is a high-quality, disease resistant and high-yielding Rehmannia glutinosa cultivar developed by the medicinal crop breeding team of National Institute of Crop Science, RDA, during the period from 1997 to 2005. The reproduction of Rehmania glutinosa has been accomplished mainly by vegetative propagation with its seedlings have many variants. The cultivar was selected from seedling of Jihwang 1. The plant type of Kokang is some rising from ground. It has higher disease resistance, catalpol content and extract content compared with Jihwang 1. Regional yield trials conducted at three site from 2003 to 2005. The root yield of Kokang was 11.8ton per hectare, which was increased 13% compared with a check variety, Jihwang 1. This cultivar is adaptable to the whole of Korea except for mountain areas.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제20권3호
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• pp.400-406
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• 2019

This study will explore the reenactment of visual special effects used in TV dramas. The subjects of the study are images that are reproduced in visual special effects through and . Accordingly, we looked at the changes in the way TV dramas were produced according to the times, and looked at the changes in the market. Also, we looked at cases of visual special effects of Korean dramas, and looked at expressions according to the degree of completion of visual special effects. For the purpose of the analysis of the research targets, the images of reproducibility and the flow through reality were analyzed. In the case of , the period setting is realistic, but visual special effects were used to maximize the actor's safety and expression, and in the case of , the visual effects were used to maximize the aesthetic of the period background. In addition, it can be seen that the visual special effects were used for the effect of PPL on the export of images. This shows that the emphasis of reality and image montage techniques are being used to pursue hyperrealism. It is now possible to actively express and use the beauty of TV dramas rather than technical limitations. In addition, the pursuit of realism can actively express the changing times of digital age. This is an active representation of the camera's angle, lighting, and perspective that coincides with the background. The environment of video production is crucial for realistic expressions. The study examined various visual directions and applications. In TV images, we looked at reproduction, which can make a natural period of time by combining. As a follow-up study, we are going to study the changes in the new quadratic image based on the present image representation.

• The Journal of the Convergence on Culture Technology
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• 제4권4호
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• pp.325-329
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• 2018

There are two kinds of calla belonging to the Zantedeschia spp. depending on the growing condition. Between them, various colored calla with flower shape and flower color are very popular as cut flowers. However, colored calla is very low in natural reproduction rate, and it takes a long time to obtain a flowering bulb. So it is urgent to establish a large breeding system of good varieties. In this study, various plant growth hormone treatments were carried out to improve the growth of the plants in calla. Root formation was the highest at 81.5 % in basal MS medium, and the formation of shoot and multiple shoots were the highest at 100.0 % and 36.4 % in the combined treatment of BA 2.0 mg/L and IBA 1.0 mg/L, respectively. In fresh weight, the highest growth rate was observed with 11.2 times increase in BA 2.0 mg/L single treatment. Auxin and cytokinin mixed treatment were widely used in previous studies and positive effects on the growth of calla plants will help to establish the micro-propagation system.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제19권12호
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• pp.878-886
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• 2018

This study quantified the sounds of riffles and pools in natural rivers and conducted a comparative analysis of the frequency and sound pressure per flow velocity. The surveyed area was Namdaecheon basin in Yangyang-gun, Gangwon-do and the sounds of a total of 23 sites were analyzed. A hydro microphone was used to measure the sound and analyze the data using an acoustic analysis program. The location was also selected at places with minimal ambient noise and the measurement points were the depth of riffles and pools. The results revealed an average difference of 0.515 m/s for flow velocity at 8 riffles and 15 pools. The difference in sound pressure occurred due to the flow velocity. In the case of sound pressure, it was measured at an average of 176.8 dB for riffles and 168.2 dB for pools, demonstrating a difference of approximately 8.6 dB. Furthermore, in the case of maximum sound pressure, riffles showed a constant range between 200 Hz and 250 Hz, while the pools exhibited maximum sound pressure at various frequencies from 200 Hz to 1,000 Hz. This revealed the ecological stream reproduction, development of preferred sound sources for aquatic life, and design of structures.

• Journal of Korean Society of Coastal and Ocean Engineers
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• 제33권3호
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• pp.110-121
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• 2021

The importance of tidal flats lost due to industrialization has recently received attention, and attention is being paid to the creation of artificial tidal flats and maintenance of natural tidal flats. However, there is still a lack of understanding about the behavioral characteristics of mud, mud, and sand that form tidal flats. Although research on the movement characteristics of mixed soils such as tidal flats has been conducted through field investigations and hydraulic experiments, interest in developing a numerical model based on these results has not yet reached. In this paper, the purpose of this paper is to establish a mixed soil model that can efficiently manage the low quality of the tidal flats. In constructing a model for reproducing the surface movement of mixed soil, the numerical stability of the reproduction and movement of sand and mud constituting the mixed soil in the numerical model should be considered first, so first, the volume of sand and mud constituting the mixed soil A mixed soil model representing the relationship was proposed based on a topographical diagram representing the geometric structure of the mixed soil. In order to consider the dry bulk density of the mixed soil, it was possible to consider the dry bulk density of the mud by introducing the water content of the mud containing water. In addition, it was confirmed that the mud and sand movement calculation according to the slope collapse of the mixed soil was stably performed through the calculation of the slope collapse of the mixed soil through the numerical analysis model to which the proposed mixed soil model was applied.

• Development and Reproduction
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• 제12권3호
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• pp.267-274
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• 2008

Differentiation of blastocyst is critical step for implantation and is under the control of regulation factors originated from embryo or reproductive tracts. The sequential communication with those factors is suspected as critical events for differentiation. It has been suggested that intracellular signaling pathways activated by calcium is essential in differentiation of blastocyst. Previously, it was known that concanavalin A (Con A) increase the levels of free calcium in blastocyst stage. However, Con A can not accelerate the hatching, although heparin-binding epidermal growth factor-like growth factor (HB-EGF), a modulator of calcium level, accelerate the hatching of blastocyst. In this study, it was investigated whether Con A or prostaglandin $E_2$ ($PGE_2$) can modulate the differentiation of blastocyst. Con A accelerated the expansion of blastocyst in both 1 hr pulse treatment group and continuous treatment group. However, Con A significantly suppressed the hatching in both groups. The inhibition was significantly strong in continuous treatment group compared with 1 hr pulse treatment group. On the other hand, $PGE_2$ induced the increase the free calcium level, but did not accelerate the expansion. In addition $10{\mu}m\;PGE_2$ inhibited hatching. However, $PGE_2$ could accelerate hatching in Con A pretreated blastocyst. $PGE_2$ also caused the increase of free calcium level in Con A pretreated blastocyst. From these results, it is suggested that changes of the free calcium level induce a different calcium-mediated signaling pathways. In addition, sequential stimulation by signal molecules may triggers the cellular mechanisms for the differentiation of blastocyst.

• Korean Journal of Plant Taxonomy
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• 제46권2호
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• pp.256-266
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• 2016

A total of 13,000 individuals of Dendrobium moniliforme (L.) Sw. artificially propagated in laboratories and greenhouses were restored in their natural habitat of Bogildo Island, Wandogun, in the southern part of Korea in June of 2013. The growing conditions of the individuals were monitored for two years. The parental individuals for the restoration were obtained from a wild population in southern Korea, from which seeds were produced via artificial crossings. These seeds were germinated and cultivated in growing media and two-year-old plants were then grown in greenhouse beds. The genetic diversity among the propagated individuals was confirmed by examining DNA sequences of five regions of the chloroplast genome and the nuclear ITS region. The diversity values were as high as the average values of natural populations. All propagated individuals were transplanted into two different sites on Bogildo by research teams with local residents and national park rangers. After restoration, we counted and measured the surviving individuals, vegetative propagated stems, and growth rates in June of both 2014 and 2015. There was no human interference, and 97% of the individuals survived. The number of propagules increased by 227% in two years. In contrast, the average length of the stems decreased during the period. In addition, different survival and propagation rates were recorded depending on the host plants and the restored sites. The shaded sides of rock cliffs and the bark of Quercus salicina showed the best propagation rates, followed by the bark of Camellia japonica. A few individuals of D. moniliforme successfully flowered, pollinated, and fruited after restoration. Overall, our monitoring data over two years indicate that the restored individuals were well adapted and vigorously propagated at the restored sites. In order to prevent human disturbance of the restored sites, a CCTV monitoring system powered by a solar panel was installed after the restoration. In addition, a human surveillance system is operated by national park rangers with local residents.