• Journal of Plant Biotechnology
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• v.3 no.3
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• pp.131-134
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• 2001

In $\lambda$-Zap II vector system, a cDNA library was constructed for the developing secondary xylem mRNA from hybrid poplar, Populus nigra x maximowiczii. A cDNA clone of 1.5 kb in size, pOMTB1.4 encoding a lignin-bispecific O-methyltransferase was screened by plaque hybridization using a probe of 540 bp cDNA amplified by polymerase chain reaction from the cDNA library and identified by nucleotide sequencing. Its nucleotide sequence contains one open reading frame of 366 amino acids. The deduced amino acid sequence in comparison with that of Populus tremuloides showed the differences of 9 amino acids and revealed 85-99% homology among alfalfa, poplar and aspen.

• Annual Conference on Human and Language Technology
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• 2018.10a
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• pp.281-284
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• 2018

본 논문에서는 machine reading 분야에서 기존의 long short-term memory (LSTM) 모델이 가지는 문제점을 해결하는 새로운 네트워크를 제안하고자 한다. 기존의 LSTM 모델은 크게 두가지 제한점을 가지는데, 그 중 첫째는 forget gate로 인해 잊혀진 중요한 문맥 정보들이 복원될 수 있는 방법이 없다는 것이다. 자연어에서 과거의 문맥 정보에 따라 현재의 단어의 의미가 크게 좌지우지될 수 있으므로 올바른 문장의 이해를 위해 필요한 과거 문맥의 정보 유지는 필수적이다. 또 다른 문제는 자연어는 그 자체로 단어들 간의 복잡한 구조를 통해 문장이 이루어지는 반면 기존의 시계열 모델들은 단어들 간의 관계를 추론할 수 있는 직접적인 방법을 가지고 있지 않다는 것이다. 본 논문에서는 최근 딥 러닝 분야에서 널리 쓰이는 attention mechanism과 본 논문이 제안하는 restore gate를 결합한 네트워크를 통해 상기 문제를 해결하고자 한다. 본 논문의 실험에서는 기존의 다른 시계열 모델들과 비교를 통해 제안한 모델의 우수성을 확인하였다.

• Proceedings of the KIEE Conference
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• 2004.11d
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• pp.97-99
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• 2004

Liquid-immersed pole transformers have utilized an insulation system consisting of natural cellulose-based materials and mineral oil for many years. If the transformers are installed with the abruptly changing load over limitation, its insulation materials are apt to unexpected aging with high temperature. Therefore we have been conscious of developing a transformer that is more thermally stable then conventional types. To measure temperature distribution in the winding of conventional and newly designed transformers, temperature sensors were installed fordirect reading of oil and conductor temperatures at transformers. And we conducted accelerating aging test for these transformers. The measuring data had made use of the design to hybrid insulation system. Also we could find the possibility of this insulation system to apply in pole mounted transformers.

• Journal of Information Processing Systems
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• v.16 no.2
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• pp.447-459
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• 2020

After reading a document, people construct a concept about the information they consumed and merge multiple words to set up keywords that represent the material. With that in mind, this study suggests a smarter and more efficient keyword extraction method wherein scholarly journals are used as the basis for the establishment of production rules based on a concept information of words appearing in a document in a way in which author-provided keywords are functional although they do not appear in the body of the document. This study presents a new way to determine the importance of each keyword, excluding non-relevant keywords. To identify the validity of extracted keywords, titles and abstracts of journals about natural language and auditory language were collected for analysis. The comparison of author-provided keywords with the keyword results of the developed system showed that the developed system was highly useful, with an accuracy rate as good as up to 96%.

• Journal of the Korea Institute of Building Construction
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• v.12 no.5
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• pp.518-527
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• 2012

This study uses a hybrid estimation tool for effective cost data management of building projects, and develops a realistic cost estimation model. The method makes use of newly available information as the project progresses, and project cost and percentage progress are analyzed and used as inputs for the developed system. For model development, case-based reasoning (CBR) is proposed, as it enables complex nonlinear mapping. This study also investigates analytic hierarchy process (AHP) for weight generation and applies them to a real project case. Real case studies are used to demonstrate and validate the benefits of the proposed approach. By using this method, an evaluation of actual project performance can be developed that appropriately considers the natural variability of construction costs.

• Animal cells and systems
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• v.6 no.4
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• pp.311-315
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• 2002

The RAD4 gene is essential for nucleotide excision repair in Saccharomyces cerevisiae. It has been known that the deduced amino acid sequence of Rad4 protein contains three DNA-dependent ATPase/helicase motifs. To determine the biochemical activities and functional role of RAD4 the Rad4 protein was expressed and purified. Immunoblot analysis showed a specific band of 21 kDa, which was well-matched with the size of open reading frame of the RAD4 gene. The purified Rad4 protein had no detectable helicase activity. However, the protein could interact with double stranded oligonucleotides, as judged by mobility shift assay. This result suggests that the Rad4 protein is a DNA binding protein.

• Child Health Nursing Research
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• v.5 no.3
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• pp.305-317
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• 1999

To understand and identity the meaning of sexuality in middle school girls and boys, the qualitative research was done. We made the in-depth unstructured interviews with 33 participants form July to October 1998. We analyzed the data according to the Collaizzi's phenomenological method. After repeated reading and interpretation of data, 15 themes and 7 basic structures were deducted. Followings were 7 basic structures: Physical touch and exposure. relationship with male and female, difference between male and female natural and inevitable life process, something should be hidden. interest and abstinence, and female as victim. According to above results, we would like to propose followings on the sexuality of middle school students : First, the research on the meaning of sexuality of high school and university students should be followed-up. Second. the measuring tool of sexual behaviour of the middle school students should be developed including the contents of 15 themes and 7 basic structures of this study.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.33 no.3
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• pp.183-187
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• 2022

Dysphonia is well known as one of the otolaryngological symptoms of coronavirus disease 2019 (COVID-19) infection. The vocal changes of the COVID-19 condition have been reported in terms of parameters of multi-dimensional voice assessment, including acoustic analysis, auditory-perceptual evaluation, and psychometric assessment. However, there has not been a daily followup study in patients with acute COVID-19 infection. In this study, a 41-year-old male performed daily voice recordings of vowel phonation and passage-reading tasks during the self-quarantine period of one week. Compared to the normal voice status of the prepandemic period, voice abnormalities peaked on day two after the diagnosis of COVID-19 infection and recovered after one week.

• The Journal of Korean Society of Virology
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• v.30 no.1
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• pp.71-81
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• 2000

We have determined and analyzed the full-length cDNA sequence of a coxsackievirus B3 (CVB3) Korean isolate (CVB3-Korea/97) which has been known as a general human pathogen. The whole genome contains 7,400 nucleotides and has a single large open reading frame with 6,555 nucleotides that encodes a potential polyprotein precursor of 2,185 amino acids. The genome also contains a 5' non-coding region (NCR) of 741 bases and a 3' NCR of 104 bases followed by poly(A) tail. Sequence homologies of nucleotides and deduced amino acids between the CVB3-Korea/97 strain and the prototype (Nancy strain) were 81.7% and 91.5%, respectively. The genes encoding the functional proteins including viral protease and RNA dependent RNA polymerase showed higher homology than those encoding the structural proteins. We have further analyzed the sequences of 5' NCR, VP1 and VP2 of CVB3-Korea/97, which are known as cardiovirulent determining factors at the nucleotide and amino acid levels. Although the CVB 3-Korea/97 strain was isolated from an aseptic meningitis patient without cardiomyopathy, its 234th nucleotide and 165th amino acid were uracil and Asn as same as those of other cardiovirulent strains one. However, the 155th amino acid of VP1, which closely associated with cardiovirulence, was replaced with $Arg^{155}$ by single nucleotide substitution from $A^{2916}$ to $T^{2916}$. Moreover, additional amino acid substitutions were observed in the flanking region of $Asp^{155}$. Taken together, amino acid(s) substitution in VP1 may playa critical role in determining cardiovirulence of the CVB3-Korea/97 strain rather than individual nucleotide replacements in the 5' NCR and/or an amino acid substitution in VP2.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.2
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• pp.71-84
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• 2014

The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.