• Journal of Korean Society of Water and Wastewater
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• v.20 no.1
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• pp.94-103
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• 2006

The pollution in closing water can be caused by not only artificial factor like sewage but also natural factor like elution from sediment. In this study we analyzed Sediment Oxygen Demand (SOD) for verification of sediment purification effect and sediment elution experiment as well as general items like COD, TN, TP, SS to complement and assess the effect of sediment and water quality. The experiment result showed that the release rate of OSD system were 4 times and 3 times as large as control for P and Fe respectively. SOD for operated OSD system and control were $12.18gO_2{\cdot}m^{-2}{\cdot}d^{-1}$ and $47.95gO_2{\cdot}m^{-2}{\cdot}d^{-1}$. From water qualities analyzed by COD, TN, TP, SS, chlorophyll-a, the removal efficiency increase of TN, TP, chlorophyll-a and COD were about 10~20%, 40~50% and 10% respectively. In conclusion, OSD can contribute to improvement of both the waterbody and the sediment environment effectively.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1690-1698
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• 2014

Partial purification of glucoamylase from solid-state fermentation culture was, firstly, investigated by reverse micellar extraction (RME). To avoid back extraction problems, the glucoamylase was kept in the original aqueous phase, while the other undesired proteins/enzymes were moved to the reverse micellar organic phase. The individual and interaction effects of main factors (i.e., pH and NaCl concentration in the aqueous phase, and concentration of sodium bis-2-ethyl-hexyl-sulfosuccinate (AOT) in the organic phase) were studied using response surface methodology. The optimum conditions for the maximum recovery of the enzyme were pH 2.75, 100 mM NaCl, and 200 mM AOT. Furthermore, the optimum organic to aqueous volume ratio ($V_{org}/V_{aq}$) and appropriate number of sequential extraction stages were 2 and 3, respectively. Finally, 60% of the undesired enzymes including proteases and xylanases were removed from the aqueous phase, while 140% of glucoamylase activity was recovered in the aqueous phase and the purification factor of glucoamylase was found to be 3.0-fold.

• Food Science and Preservation
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• v.7 no.2
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• pp.201-206
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• 2000

${\beta}$-Galactosidase was extracted and purified from strawberry. The purified ${\beta}$-Galactosidase from strawberry was investigated their physicochemical characteristics. ${\beta}$-Galactosidase was purified 25.74 fold from strawberry. The purification procedure include ammonium sulfate fraction, acetone powder treatment and gel and ion exchange chromatography. Yield of the enzyme purification was 18.11%. The purified enzyme has native molecular weight of 116,000 dalton. Vmax value and Km value of ${\beta}$-Galactosidase were 0.077 mM ONPG/ml/15mim and 1.75x10-2mM, respectively. The optimum temperature and pH of ${\beta}$-Galactosidase were 43$^{\circ}$C and pH 4.0, respectively. The ${\beta}$-Galactosidase activity was stable below 50$^{\circ}$C and at pH 4.0 to pH 6.0. Among the metal ions Ca and Mg were did not affect, whereas K, Cu and Zn show a little effect on the enzyme activity. The ${\beta}$-Galactosidase activities were inhibited by treatment with EDTA and SDS.

• Journal of Integrative Natural Science
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• v.6 no.4
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• pp.193-196
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• 2013

Haemoglobin is an interesting physiologically significant protein composed of specific functional prosthetic haem and globin moieties. In recent decades, there has been substantial interest in attempting to understand the structural basis and functional diversity of avian haemoglobins (Hbs). Towards this end, purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies have been carried out on Amaurornis phoenicurus Hb. Crystals were grown by the hanging drop vapor-diffusion method using PEG 2000 and NaCl as precipitants. The crystals belonged to the primitive monoclinic system $P2_1$, with unit-cell parameters $a=65.33{\AA}$, $b=93.14{\AA}$, $c=98.54{\AA}$, ${\beta}=100.48^{\circ}$; a complete data set was collected to a resolution of $2.6{\AA}$. The Matthews coefficient of $2.30{\AA}^3Da^{-1}$ for the crystal indicated the presence of two ${\alpha}_2{\beta}_2$ tetramers in the asymmetric unit.

• Journal of the Korean Institute of Gas
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• v.23 no.2
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• pp.61-67
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• 2019

Korea is natural gas importer which imports a lot quantities which 20% of the volume of US exports in 2018. Biogas which can satisfy gas demand and respond effectively to climate change, will be an alternative. However, only 20% of biogas production is sold, which is also not efficient and difficult to use. The purpose of this study develops an optimal purification system for supplying biogas as city gas. We develope an optimal system by analyzing biogas for system selection, finding cases for system design, developing scenario, and developing a cost - benefit tool.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2001.04a
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• pp.59-59
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• 2001

No Abstract. See Full-text

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.174.3-175
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• 2001

• Proceedings of the Ginseng society Conference
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• 1978.09a
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• pp.13-17
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• 1978

This paper is concerned with the studies on the effective components of anti-oxidant activity, with a view to demonstrate the anti-aging activity of Korean ginseng. Feeding the extract of Korean ginseng or its effective component to mice inhibited strongly the induction of lipid peroxidation produced by ethanol intoxication. From the extract of Korean red ginseng, one effective component Compound A, mp.143, $C_6H_6O_3$ was isolated by chromatographic purification and its chemical structure was determined as 2-meth-y1-3-hydroxy-${\gamma}-pyrone(maltol).$

• Natural Product Sciences
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• v.18 no.3
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• pp.166-170
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• 2012

The purification of the MeOH extract from the aerial parts of Erythronium japonicum using column chromatography furnished a new megastigmane glucoside, erthrojaponiside (1), together with six known megastigmane derivatives (2 - 7). The structure of the new compound (1) was determined through 1D and 2D NMR spectral data analysis and chemical means. The isolated compounds (1 - 7) were tested for cytotoxicity against four human tumor cells in vitro using a Sulforhodamin B (SRB) bioassay.

• Journal of the Korea Organic Resources Recycling Association
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• v.8 no.1
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• pp.77-82
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• 2000

Seven constructed and three natural tidal flats were compared to evaluate state-of- the-art of creation and restoration technology for tidal flats. parameters studied were physico-chemical and biological characteristics of soils and rate of respiration. The natural tidal flats had higher contents of silts, nitrogen and organic matter compared to the constructed ones. The natural ones had reductive Bone below 2 cm whereas the constructed ones had oxidative zone from the surface to below 20 cm. The bacterial population in the soil of the constructed tidal flats was one to two magnitudes lower than that in the natural ones. Biomass of macrobenthos and microbial respiration rate, however, were not different significantly between the natural and the constructed tidal flats. The purification capacity by diatom+bacterial+meiobenthos and macrobenthos in the constructed tidal flats was higher than that in the natural ones due to deeper permeable layer for purification in the constructed tidal flats. There was an exceptional constructed tidal flat with similar physico-chemical and biological characteristics to natural ones. Shearing stress to the surface of the tidal flat by the flow of seawater was as low as that of natural ones. These hydraulic conditions seemed to be a controlling factor on structures and functions of tidal flats. The control of hydraulic condition seemed to be one of the most important factors to create natural-like tidal flats.