• 한국응용곤충학회지
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• 제51권4호
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• pp.371-376
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• 2012

본 시험은 온도 발육모델을 이용하여 꽃매미 알의 부화시기를 예측하고 약충과 성충 방제에 효과적인 친환경 자재선발 및 끈끈이트랩 색상별 유인효과를 찾기 위하여 수행하였다. 꽃매미 알은 15, 20, $25^{\circ}C$(14L:10D)에서 각각 55.9, 26.8, 21.6일 만에 부화하였고 부화율은 각각 61.9, 57.8, 30.4%로 온도가 높을수록 부화소요기간은 짧고 부화율은 낮았다. 알의 온도와 발육속도와의 관계식은 Y=0.0028X-0.0228 ($R^2$=0.9561)이었으며, 발육영점온도는 $8.14^{\circ}C$, 유효적산온도는 355.4일도였다. 위의 관계식에 의하여 전남지역 꽃매미 알의 부화시기는 5월 22일로 예측되었다. 꽃매미 약충방제에 효과적인 친환경자재는 제충국추출물, 고삼추출물, 데리스추출물이었고, 성충은 제충국추출물, 고삼추출물이었다. 황토색, 파랑색, 노랑색 끈끈이트랩 중에서 황토색끈끈이트랩의 유인량은 2주 동안에 약충 535마리, 성충 87.7마리였다. 황토색 끈끈이트랩은 꽃매미 약충과 성충의 유인포살효과가 우수하였다.

• Clinical and Experimental Reproductive Medicine
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• 제27권3호
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• pp.283-289
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• 2000

Objective: The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. Methods: We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo. Results: We tested toxicity by exposing embryos to vitirification solutions and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93.0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9% (EFS), 48.5% (VS14) and 70.1% (UFS). Conclusion: The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.

• 한국발생생물학회지:발생과생식
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• 제16권3호
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• pp.219-226
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• 2012

It is suggested that carbohydrate metabolites may involve in the development of morula to blastocyst but many of the mechanisms are not unmasked. Two-cell stage embryos were collected and examined the effects of lactate on the development of blastocyst in vitro. The expression profiles of lactate dehydrognase (Ldh) genes and aquaporin (Aqp) genes were analyzed with RT-PCR. The successful development from morula to blastocyst was dependent on lactate concentrations. The expression profiles of Ldh genes were changed by the lactate concentration. Ldha was expressed in morula stage at 10 mM lactate, and in blastocyst stage at lactate free condition. Ldhb was expressed in morula stage at 10 mM and 20 mM lactate, and in blastocyst stage at 10 mM lactate. Aqp genes were also showed different expression patterns by the lactate concentrations. Aqp3 was expressed in hatching embryo at 120 hr post hCG administration (hph) which was cultured in BWW medium and lactate free condition. Aqp7 was expressed in hatching embryos at 120 hph which was cultured at 10 mM lactate condition. Also Aqp8 was expressed in hatching embryo at BWW and 20 mM lactate condition. Aqp9 was expressed in morula at BWW and 10 mM lactate condition, and in blastocyst at BWW. Based on these results, it is suggested that concentration of lactate in the medium and the level of lactate synthesis in embryo is critical factor for blastocoels formation. In addition it is suggested that LDH may involve the AQPs expression in embryos.

• 한국수정란이식학회지
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• 제4권1호
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• pp.41-45
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• 1989

Total thirty of flushing were attempted on day 4 to 15 of estrus cycle with S heifers and 9 cows by nonsurgical method. The flushed or recovered rate among flushings was 86.7% (26/30) or 88.5% (23/26), respectively. There was no difference in the recovered rate between heifers (85.7%,6/7) and cows (89.5%, 17119). The embryo was recovered on day 4 to 15 of estrus cycle from the donors in natural heat without any technical difficulties.The I2FG Foley catheter used for pubertal heifers had sometimes plug in it with uterine mucus during flushing of uterine horn. But the problem could be overcomed by pumping the catherter with fluthing solution or by changing the catheter. Three normal embryos were recovered from 3 pubertal (10-11 month old) heifers. The rate of normal and abnormal eggs was 60.9% (14123) and 39.1% (9/23), respectively. The abnormal eggs were on degenerating except one unfertilized egg and were mostly recovered from heifers or cows flushed consecutively during the estrus cycle. The developmental states of normal embryos were l6-cells on day 5, 32-cells on day 6, compacted-morula on day 7, early-to expanded-blastocyst on day 8-to 9, and hatching-to hatched-blastocyst on day 10 to 11 of estrus cycle. The stage of embryos on day 8 to 10 showed varities among donors. On day 8 to 9 of estrus cycle hatching-blas tocyst was recovered from some donors.

• 한국발생생물학회지:발생과생식
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• 제18권3호
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• pp.173-178
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• 2014

In order to monitor the developmental features of embryos, larvae, and juveniles of Oryzias latipes (Temminck and Schlegel), Oryzias latipes was caught in river of Shinduck-dong, Yeosu-si, Jeollanam-do, on May 2011, and experiments were carried out in Ichthyology laboratory at Chonnam National University. The blastodisc step was the first level for natural spawning. The optic vesicle, Kupffer's vesicle, myotome began to appear 75 hours 57 minutes later. After blastodisc development, the pectoral fins were made at 143 hours 37 minutes and the tail was separated started at the same time. Hatching was observed at 167 hours 27 minutes after blastodisc. The total length of the hatched larvae was 4.95~5.10 mm (mean, 5.01 mm), the mouth and anus were opened. Larvae used yolk completely after 3 days after hatching. The total length larvae was 5.45~5.56 mm (mean, 5.52 mm) after 8 days after hatching, and appeared the stems for tail. The stems pectoral, anal fin were showed after 14 days and the stems dorsal, ventral fin were appeared after 19 days. For 35 days after hatching, the total length of larvae 13.95~15.30 mm (mean, 14.64 mm), and at this time, fins and body were transferred like the adult Oryzias latipes.

• 한국환경생태학회지
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• 제22권1호
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• pp.59-65
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• 2008

본 연구는 2005년부터 2007년까지 파주, 강화도, 이천, 시화호, 충주 등 5개 지역에 서식하는 수리부엉이 9쌍의 둥지를 정기적으로 촬영, 관찰하여 번식생태와 교미행동을 연구하였다. 한배산란수는 $1\sim4$개로 평균 2.77개(n=15)였으며, 부화성공률은 약 71%(n=34)였고, 이소성공률은 약 83%(n=23)였다. 수리부엉이는 산란이 끝난 후에도 암수간의 교미행동이 지속되며, 새끼들이 둥지를 떠나는 시기인 부화 후 $7\sim8$주까지 교미행동을 계속한다는 것을 확인하였다. 이러한 수정 목적 이외의 교미 행동은 암컷과 수컷 모두의 필요에 의해서 이루어지는 것으로써 '부부관계(pair-bond)'의 확인, 유지, 강화를 통해 새끼를 안정적으로 키우기 위한 것으로 해석된다.

• The Korean Journal of Ecology
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• 제19권6호
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• pp.575-582
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• 1996

Molipteryx fuliginosa (Uhler, 1860) is a plant juice sucker which feeds on new tips of Rubus oldhami Miquel and Zelkowa serrata Makino, and it has a strong preference for teses two plants in Korea. M. fuliginosa has one generation a year and hibernates as a young adult. Most of the winter survivors emerge in early May. It is the first time their host plants were found and reported. Females mainly lay their eggs one by one separately on the leaves of R. oldhami, and even on the steel wire, the lid guaze and the ground in the laboratory. Nymphs do not gather, but stay on the hatching site, Nymphs except the non-feeding first instar feed on young shoots. From the second to the fifth instar nymphs migrate to the upper part of the shoot and congregate in part on an expanded leaf. The new adults first appeared on 11 August, and remained in the host plant, and fed on until mid October. The duration of the hatching and molting, and the survivorship curve based on the laboratory rearing were determined.

• 대한수의학회지
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• 제38권3호
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• pp.450-454
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• 1998

We have studied Meckel's diverticulum(MD) of the duck(Anas platyrhynchos platyrhyncos, Linne) by histological and immunohistochemical methods. Because MD were first observed in 2 weeks after hatching, tissues were taken from 2 weeks to 32 weeks after hatching groups. MD were observed as like any parts of intestinal tract that composed with simple columnar epithelium and numerous mucosal gland especially, cecum except that many lymphocytes were exist in this study. Also a number of bovine chromogranin(BCG)-, serotonin, and somatostatin(SOM)-immunoreactive cells were observed in epithelium and submucosal gland in this study, so it could be suggest that the MD of the duck serve as some digestive and lymphatic functions.

• 한국발생생물학회지:발생과생식
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• 제12권3호
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• pp.267-274
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• 2008

포배의 분화는 배아의 착상에 있어 핵심적인 단계로 배아 자체 또는 생식수관에서 유래하는 조절요인의 조절을 받는다. 이들 조절요인과 포배와의 순차적인 신호의 주고 받음은 분화의 중요한 단계로 인식되고 있다. 한편, 포배기 때 자유 칼슘을 통한 신호전달경로가 포배의 분화에 중요한 축의 하나로 제안되어 왔다. Concanavalin A(Con A)가 포배의 자유 칼슘 농도 증감을 유도한다는 것을 밝혀졌으나, 포배 내 자유 칼슘 농도를 변형시켜 부화와 그 이후의 발생을 촉진하는 것으로 알려진 heparin-binding epidermal growth factor-like growth factor(HB-EGF)와는 달리 팽창 이후의 부화를 억제하였다. 따라서 본 연구에서는 착상과정에서 중요한 역할을 하는 것으로 알려진 prostaglandin $E_2(PGE_2)$가 포배의 분화에 관여하는지를 Con A와 연계하여 알아보았다. Con A는 그 처리 시간에 관계없이 1시간 처리군 그리고 계속처리군에서 팽창은 촉진하고 부화는 유의하게 억제하였다. 특히 계속처리군에서 부화율이 1시간 처리군에 비하여 유의하게 감소하였다. 또한, $PGE_2$도 포배 내 자율 칼슘 농도를 증가시켰으나 팽창과 부화를 촉진하지 않았다. 또한, $10{\mu}m\;PGE_2$ 농도에서는 부화가 억제되는 경향을 보였다. 그러나 흥미롭게도 $PGE_2$는 Con A가 처리된 포배의 부화를 촉진하였다. Con A를 전처리한 포배에 $PGE_2$를 처리할 경우 포배 내 자유 칼슘의 농도 증감이 진행됨을 공촛점현미경을 이용하여 분석할 수 있었다. 이러한 결과는 신호물질에 의해 유도된 자유 칼슘 농도의 증감이 신호물질에 따른 각기 다른 칼슘 매개로 활성화되는 신호경로를 조절하는 것을 추정할 수 있다. 또한, 순차적 신호물질 조절에 의한 자유 칼슘의 농도 증감이 포배의 분화에 있어 중요함을 제안한다.

• 한국산림과학회지
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• 제95권2호
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• pp.174-176
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• 2006

The experiments were conducted by supplying hosts with natural food(chestnut, peach, Quince). The developmental difference of peach pyralid moth, Dichocrocis punctiferalis was examined in the laboratory under three different natural food regime. The periods of egg, larva and pupa were $6.01{\pm}0.07$, $12.23{\pm}0.03$ and $13.32{\pm}0.01$ days on the chestnut fruit, $6.21{\pm}0.01$, $18.69{\pm}0.02$ and $13.38{\pm}0.03$ days on the peach fruit and $7.02{\pm}0.04$, $22.62{\pm}0.04$ and $13.44{\pm}0.14$ days on the quince fruit, respectively. The growth of D. punctiferalis larva was better chestnut fruit than other tested fruits. The rates(%) of hatching, pupation and emergence were 94.0, 57.0 and 63.3 on the chestnut fruit, 89.2, 77.8 and 85.7 on the peach fruit and 79.6, 52.6 and 70.7 on the quince fruit, respectively. The survival rate(%) of D. punctiferalis from hatching to emergence were 31.0 on the chestnut fruit, 4.8 on the peach fruit and 14.3 on the quince fruit, respectively. The sex ratio (female: male) of all pupae obtained on the tested natural food fruits were 52.7 : 47.3. The sex ratio of D. punctiferalis reared on three difference food fruits were no significantly. It can be used a as the basic research for the study of D. punctiferalis.