• 미생물학회지
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• 제26권2호
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• pp.82-87
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• 1988

The genetic analysis and characterization of protoplast fusion hybrids between complementary auxotrophic mutants of Candida pseudotropicalis were carried out. Nuclear fusion appeared to occur in fusion hybrids (e.g., F15 and F33), as strongly suggested by isolation of recombinants after mitotic segregation of parental genetic markers. This was confirmed by KNA content, nuclear staining and comparison of survival rate to UV light. After keeping fusion hybrids for approximately one year, the frequency of spontaneous mitotic segregation was $3.0\times 10^{-4}$ - $8.1\times 10^{-4}$ while that of induced mitotic segregation was $1.4\times 10^{-3}$- $1.7\times 10^{-3}$. These results suggested that they maintained stable karyogamy state. It was also found that the production of $\beta$-D-galactosidase from F15, F33 and F158 was somewhat increased when compared with that from either auxotrophic parents or wild type.

• 동력기계공학회지
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• 제8권2호
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• pp.53-58
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• 2004

In this paper, the semi active mount featuring Magneto rheological fluid(MR Fluid) is proposed. MR fluid is suspension of micro sized magnetizable particles in a fluid medium, and its apparent viscosity can be varied by the applied strength of magnetic field. When the controllable MR fluid is applied to mechanical devices, the devices provide simple, rapid response interfaces between electronic controls and mechanical systems. The MR fluid is applied in the conventional fluid mount for improving its performance of the mount's isolation effect. A appropriate size of the MR mount is designed and manufactured on the basis of Bingham model of MR fluid. In addition, the field dependent damping forces of MR mount are evaluated with respect to the input frequency variation.

• 동력기계공학회지
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• 제18권2호
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• pp.77-82
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• 2014

The air spring is widely used in various fields such as a suspension system and an anti-vibration system because the natural frequency is kept constant regardless of the change in the load, spring constant is easy to change, and, vibration and shock isolation performance are excellent. The purpose of this study is to derive a nonlinear governing equation of an air spring, to analyze the effect of the various parameters on the dynamic stiffness of the air spring, and, to suggest a more efficient design method of an air spring system. In order to do so, this study investigates the impact of all the parameters that could affect the dynamic stiffness of the air spring while changing the excitation amplitude and the frequency with a developed governing equation.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2004년도 추계학술대회논문집
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• pp.97-102
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• 2004

The Characteristics of noise and vibration by heavy-weight floor impact sound was studied. Resonance frequency increased a little in structures that use damping material in living room and bedroom, and acceleration waves length that respond became short, and displayed aspect that oscillation level decreases. Result that measure sound pressure level, structure that compare and applies damping materials with structure that apply the resilient materials from 63Hz lower part that impact energy is concentrated in energy spectrum of heavy-weight floor impact sound displayed result that sound pressure, level decreases remarkably. Therefore, according to use of damping materials, confirmed reduction effect of heavy-weight floor impact sound.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2003년도 추계 학술발표회논문집
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• pp.326-333
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• 2003

Most long-span bridges such as a cable-stayed bridges have a number of long-period modes due to the flexibility, thus the design concept extending the natural period of structures using base isolation system may be difficult to use directly to these structures. But, the effectiveness of LRB for cable-stayed bridges is indicated in several papers. In this study, the guidelines of designing LRB for a seismically excited cable-stayed bridge using benchmark cable-stayed bridge are presented. The design properties of LRB are chosen that the design index(DI) is minimized or little changed for variation of properties. And the seismic performance of designed LRB is also investigated. The consequences show that the perforamnce of designed LRB is better than that of simply designed LRB for several history earthquakes. Moreover, the design properties of LRB are researched to several diffrent dominant frequency of earthquake. The results present that the plastic and elastic stiffness of LRB are affected by the dominant frequency of earthquake.

• 음성과학
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• 제13권3호
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• pp.67-82
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• 2006

The purpose of this experimental study is to investigate formant characteristics of Korean monophthongs spoken by Japanese learners and to compare the characteristics of vowels produced by the Japanese learners with those of the Korean native speakers. The data consisted of three categories: eight vowels in isolation, words including eight vowels in carrier sentences, and words including eight vowels in natural sentences. In this study, formant frequencies of the vowels were measured by Wave Surfer. It was assumed that the formant frequencies of the Korean vowels produced by the Japanese learners could be different from those of the Korean native speakers due to the influence of their own Japanese vowels. Results of this study showed that the Japanese learners had the difficulties to distinguish between the pairs /-/and /ㅜ/, /ㅓ/and /ㅗ/, and /ㅏ/and /ㅔ/. In Japanese vowels, F2 frequency value of /ㅜ/ was similar to that of the Korean /-/. It means that when the Japanese leaners produced Korean /ㅜ/, they might neutralize /-/ and /ㅜ/. Besides, there were not /ㅓ/and /ㅐ/ in Japanese vowels. Therefore, they tended to pronounce /ㅓ/ similar to /ㅗ/ which has the most similar formant frequency value with that of /ㅓ/, and /ㅐ/ was pronounced similar to /ㅔ/ for the same reason.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2023년도 가을학술발표대회논문집
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• pp.227-228
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• 2023

A floating floor generally consists of mortar bed separated from the structural RC slab by a continuous resilient layer. It is known that the floating floors are a type of vibration-isolation system to improve the impact sound insulation performance. However, some researchers have demonstrated that the amplification of vibration response at a specific range of frequencies results in an increase in the impact sound level. This study carried out the forced vibration tests to obtain the frequency response function (FRF) of a floating floor compared with a bare RC slab. Test results shows that the additional peak occur in vibrational spectrum of the floating floor except natural vibration modes of the bare RC slab. This is because the relatively flexible resilient material and mass of the mortar bed offer an additional degree of freedom in the structural system. Therefore, it could be efficient for reduction of floor impact vibration and noise to control the additional mode frequency and response of floating floors.

• 대한미생물학회지
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• 제21권3호
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• pp.345-353
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• 1986

Authors studies on the isolation of non-sucrose fermentation Vibrio species such as V. parahaemolyticus, V. vulnificus and V. damsela from sea water, shellfishes and various algae at Ulleung island in the east of Korea on summer in 1985. Authors carried out test for isolated strains to biochemical characteristics, halophilism, hemolysis and serological reaction. The results obtained were as follows: 1. Strains isolated from total specimens were 25 strains of V. parahaemolyticus, 2 strains of V. vulnificus and 1 strain of V. damsela, respectively. 2. Isolated frequency of Vibrio species from sea water and marine products was lower than on specimens in the southern sea of Korea from 1980 to 1985. 3. On hemolysis reaction on blood agar media using human and rabbit erythrocytes, 14 strains among 25 strains of V. parahaemolyticus isolated were positive, and V. vulnificus and V. damsela were positive, respectively. 4. The distributions of serotypes of V. parahaemolyticus isolated were from O1 group to O10 group, and from K 10 to K 69. The results of K serotypes were as follows ; serotype K 10 and K 33 were 2 strains, serotype K 59 and K 69 were 1 strain, respectively. And 2 strains of V. vulnificus isolated were not agglutinated by antiserum of V. vulnificus. Therefore, the probability is that 2 strains of V. vulnificus isolated were other serotypes distributed on sea weater and marine products of Ulleung island of Korea.

• 한국유기농업학회지
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• 제6권1호
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• pp.133-149
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• 1997

Recently, the importance of management and cultivation of grasses has been increased in Korea. Among these cultural practices, the appropriate control of diseases is considered more important than other cultivation techniques such as fertilization and irrigation. The damages of brown patch and large patch caused by Rhizoctonia spp. and Pythium blight caused by Pythium spp. are serious in the major cultivation area of turfgrass in Korea. Since these diseases are difficult to control by agrochemicals, the damages are very serious if these are occured. The periodic spray of agrochemicals, to protect and control these diseases could make some problems of toxicity and environmental pollution as well as rising of non-target diseases. Therefore, the biological methods to control diseases have been required to decrease problems resulted from overuse of agrochemicals, to conserve natural ecosystem, and to control effectively diseases of grasses in the long period. The number of studies about biological control using antagonistic microorganisms have been increased for last half century. However, the application of biological control method has been very limited. In this study, thirteen isolates of R. cerealis, 8 isolates of R. solani and 3 isolates of Phthyn spp. have been isolated from diseased turfgrass in golf course and grass-culture area that have patch and wilting symptoms of zoysia grass and creeping bentgrass. Isolation frequency of R. cerealis and R. solani was high in especially zoysiagrass, while Pythym spp. was isolated from bent grass at low frequency but showed high pathogenicity. Totally, 205 isolates of soil microorganisms were isolated in this study as primary antagonistic microorganism by Herr's triple agar layer plate and dual culture method using rhizosphere of grasses, soil of crop field as the source of antagonistic microorganisms. Among the 205 isolates, 23 isolates were actinomycetes and 182 isolates were bacteria. All of the actinomycetes were isolated by Herr's method. Antagonistic effect of primary isolated microorganisms was tested for in vitro mycelial growth inhibition against pathogenic fungi isolated from grasses and for inhibition of disease occurrence in 24 well tissue culture plate and pot experiment. Then, four isolated of bacteria which are BG23, BG74, BG136 and BG171 were selected as antagonistic microorganisms against soil-born pathogenic fungi of bentgrass.

• 한국산학기술학회논문지
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• 제17권5호
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• pp.601-611
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• 2016

최근 빈번하게 발생하는 크고 작은 지진에 대비하여 구조물의 지진에 대한 안전성을 확보하기 위한 방안으로 지진격리시스템을 이용한 연구개발과 사용이 점차 증가하고 있다. 지진격리시스템의 하나인 고감쇠 고무받침(HDRB)는 특수한 고감쇠 고무(HDR)를 이용한 적층형 고무받침으로서 유사 지진격리장치인 납 고무받침에 비해 감쇠기능이 다소 떨어지는 단점이 있어 활용도가 높지 않았다. 그러나, 고감쇠 고무받침은 재료와 형상이 유사한 천연고무받침의 비해 우수한 감쇠력을 가지고 있으며 기존 납 고무받침의 경우 납의 유해성이 문제되어 납을 사용하지 않은 고감쇠 고무받침에 대한 연구가 증가하고 있다. 본 연구에서는 고감쇠 고무받침을 대상으로 압축응력 의존성 및 주파수 의존성, 반복하중 의존성 등 다양한 특성에 대하여 실험을 실시하였다. 그리고 여러 계기지진파 상태에서 고감쇠 고무받침의 내진성능을 평가하기 위해 진동대 실험과 분석을 수행하였다. 축소교량에 고감쇠 고무받침을 적용한 모델을 사용했고, 지진격리와 비 지진격리로 구분하여 진행하였다. 그 결과 고감쇠 고무받침을 적용할 경우 비 지진격리의 경우에 비해 높은 감쇠효과를 보였으나 Mexico City와 같은 연약지반의 구조물에 지진격리를 적용할 경우 오히려 구조물의 응답이 증가하는 양상을 나타내 지진격리장치 적용성에 주의해야 할 것으로 판단된다.