• Journal of Korean Society on Water Environment
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• v.34 no.1
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• pp.57-66
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• 2018

Water disasters such as flash floods and inundation caused by localized heavy rainfall in urban areas have a large impact on climate change but are also closely related to the increase in impervious areas as pointed out in domestic and international studies. It is difficult to secure natural green areas in urban areas that have already been developed. So, urban regeneration can be expected using water management optimized with technologies to secure infiltration and storage capacity such as Low-Impact Development technology. In this study, the water cycle improvement ability was confirmed by applying the LID technology within the district unit plan of the environmentally friendly village, and the economic feasibility of LID application was analyzed by estimating the costs and benefits of installing the facilities. The site was planned to conserve sufficient green and plans for securing the watershed infiltration and storage capacity were formulated with the application of additional LID technology, such as infiltration trenches, rain barrels and permeable pavements. The LID design method applicable to the site was established, and the water balance of the watershed was analyzed through simulations of the SWMM model. The water circulation improvement effect was confirmed through the water balance analysis, and the cost-benefits were determined according to the estimation method, and the economic analysis was conducted. This study confirms that the investment of LID technology is economically feasible for the hydrological improvement effect of the housing complex.

• Journal of Climate Change Research
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• v.8 no.4
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• pp.287-295
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• 2017

This paper provides a mathematical approach for estimating flood risks due to the effects of climate change by developing a one dimensional (1D) hydraulic model for the mountainous river reaches located close to the Yeongwol thermal power plant. Input data for the model, including topographical data and river discharges measured every 10 minutes from July $1^{st}$ to September $30^{th}$, 2013, were imported to a 1D hydraulic model. Climate change scenarios were estimated by referencing the climate change adaptation strategies of the government and historical information about the extreme flood event in 2006. The down stream boundary was determined as the friction slope, which is 0.001. The roughness coefficient of the main channels was determined to be 0.036. The results show the effectiveness of the riverbed widening strategy through the six flooding scenarios to reduce flood depth and flow velocity that impact on the power plant. In addition, the impact of upper Namhan River flow is more significant than Dong River.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.1438-1441
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• 2008

Throughout the history, the people of Iran have battled the dryness by innovations to preserve every drop of water that lands from the rare clouds, or from a stream flowing out of distant springs. Water is precious and held with highest respect, whether stored for drinking at an Ab-Anbar, or for washing and farming at the Houz in the middle of their oasis homes and orchards, or sourced at a Qanat spring or Jooy under ground. How it is that drinking water as cold as a mountain fall is found in desert of Iran? Ab-Anbar is an ancient means of water preservation and cooling through anunderground building structure. These underground structures have been present in Khorasan and other desert provinces of Iran as public or private water storage facilities, widely used before the installation of public plumbing systems in the late 1950s. Although many of these structures are still functional, most have been protected by government for restoration or viewing by the public as historical heritage. Khorasan natural dry climate and the massive surrounding deserts have been a breeding ground for many designs of Ab-Anbars. Today the existing number of such facilities stands in the province of Khorasan. Usually these structures were built in populated areas, also there are some forms of such structures on old trade routes and roadways leading to and from populated towns. This paper considers the history of Ab-Anbars in Khorasan as well as other relevant aspects such as types, components, construction methods and materials, filling and withdrawal systems.

• Molecules and Cells
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• v.41 no.5
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• pp.413-422
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• 2018

Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from $T_2$ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than ${\underline{n}}on-{\underline{t}}ransgenic$ (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were significantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In microscopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants ($T_2$) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.271-276
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• 2018

Phytoplanktons were collected from various environments including small marshes, small ponds, reservoirs and brackish water from March 2017 to November 2017. In this study, five species of family Scenedesmaceae were newly recorded in Korea. The genus Desmodesmus was newly reported in Korea. The newly recorded species are Scenedesmus nanus, S. praetervisus, Desmodesmus costato-granulatus, D. lunatus and D. spinulatus. S. nanus and S. praetervisus are known to occur in freshwater, but they were found in the brackish water in this study.

• Korean Journal of Environmental Agriculture
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• v.37 no.3
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• pp.172-178
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• 2018

BACKGROUND: Composted animal manure applied to the arable soil for improving soil quality and enhancing crop productivity causes greenhouse gas emissions such as nitrous oxide ($N_2O$) by processes of nitrification and denitrification. However, little studies have been conducted on determining effect of application ratio of composted animal manure on $N_2O$ emission rate and its annual emission pattern from upland soil in South Korea. Therefore, this study was conducted to determine $N_2O$ emission rate and its annual emission pattern from upland soil supporting for sweet potato. METHODS AND RESULTS: Composted animal manure was applied at the ratio of 0, 10, and 20 Mg/ha to an upland soil supporting for sweet potato (Ipomoea batatas). Nitrous oxide emission was examined during growing season and non-growing season from May 2016 through May 2017. Daily $N_2O$ fluxes showed peaks right after applications of composted animal manure and inorganic nitrogen fertilizer. Precipitation and soil water content affected daily $N_2O$ flux during non-growing season. Especially, $N_2O$ flux was strongly associated with water filled pore space (WFPS). We assumed that the majority of $N_2O$ measured during growing season of sweet potato was produced from nitrification and subsequent denitrification. Annual cumulative $N_2O$ emission rate significantly increased with increasing application ratio of composted animal manure. It increased to 12.0 kg/ha/yr from 8.73 kg/ha/yr at control with 10 Mg/ha of composted animal manure and to 14.0 kg/ha/yr of $N_2O$ emission with 20 Mg/ha of the manure. CONCLUSION: To reduce $N_2O$ emission from arable soil, further research on developing management strategy associated with use of the composted animal manure and soil moisture is needed.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.153-153
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• 2019

Evapotranspiration (ET) is an important component of hydrological processes. Accurate estimates of ET variation are of vital importance for natural hazard adaptation and water resource management. This study first developed a soil water index (SWI)-based Priestley-Taylor algorithm (SWI-PT) based on the enhanced vegetation index (EVI), SWI, net radiation, and temperature. The algorithm was then compared with a modified satellite-based Priestley-Taylor ET model (MS-PT). After examining the performance of the two models at 10 flux tower sites in different land cover types over East Asia and Australia, the daily estimates from the SWI-PT model were closer to observations than those of the MS-PT model in each land cover type. The average correlation coefficient of the SWI-PT model was 0.81, compared with 0.66 in the original MS-PT model. The average value of the root mean square error decreased from $36.46W/m^2$ to $23.37W/m^2$ in the SWI-PT model, which used different variables of soil moisture and vegetation indices to capture soil evaporation and vegetative transpiration, respectively. By using the EVI and SWI, uncertainties involved in optimizing vegetation and water constraints were reduced. The estimated ET from the MS-PT model was most sensitive (to the normalized difference vegetation index (NDVI) in forests) to net radiation ($R_n$) in grassland and cropland. The estimated ET from the SWI-PT model was most sensitive to $R_n$, followed by SWI, air temperature ($T_a$), and the EVI in each land cover type. Overall, the results showed that the MS-PT model estimates of ET in forest and cropland were weak. By replacing the fraction of soil moisture ($f_{sm}$) with the SWI and the NDVI with the EVI, the newly developed SWI-PT model captured soil evaporation and vegetation transpiration more accurately than the MS-PT model.

• Journal of Marine Bioscience and Biotechnology
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• v.1 no.4
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• pp.268-274
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• 2006

Bacillus sp. DYL130 producing biosurfactant was isolated from soil samples in the Duck-yu mountain and identified as Bacillus sp. by analysis of 16S rDNA sequence. Purification of the biosurfactant was performed by using affinity chromatography and TLC. The biosurfactant of culture medium from Bacillus sp. DYL130 was eluted with 100% methanol using affinity chromatography. To remove methanol, a rotary evaporator was used and enrichment sample was dissolved in alkaline water(pH 10). The purified biosurfactant was identified by TLC. It was confirmed that the Rf value of the biosurfactant was 0.78. Antifungal activity against Botrytis cineria was showed the strongly activity as active antagonist. Maximum emulsification activity and stability were obtained from soybean oil. The critical micelle concentration (CMC) of purified biosurfactant was 35mg/l and the purified biosurfactant inhibited biofilm forming by Bacillus sp..

• Journal of Species Research
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• v.11 no.4
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• pp.321-329
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• 2022

Four species of cyanobacteria that are unrecorded in Korea were isolated from freshwater and brackish water. These four species are Laspinema thermale of Laspinemaceae, Planktothricoides raciborskii and Planktothrix spiroides of Microcoleaceae, and Cephalothrix lacustris of Phormidiaceae, all belonging to the order Oscillatoriales. Laspinema thermale is morphologically characterized as apical cells that are longer than other cells. In this strain, the similarity of the 16S rRNA gene sequence with the previously reported L. thermale strains were 99.30-99.50%. Planktothricoides raciborskii, which is characterized by bluntly conical morphology of apical cells, showed 98.80-99.50% of similarity of the 16S rRNA gene sequence to the previously reported P. raciborskii strains. Planktothrix spiroides are characterized by floating due to gas vacuoles. In this strain, the similarity of the 16S rRNA gene sequence with the previously reported P. spiroides strains were 99.80-99.90%. Cephalothrix lacustris, characterized by having calyptra in apical cells, showed 99.80-99.90% similarity of the 16S rRNA gene sequence to previously reported C. lacustris strains. Also, these species were clustered in the same clade in phylogenetic analysis using 16S rRNA gene sequences with each corresponding species.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.211-221
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• 2012

The fourth leaves (younger leaves) amongst extended 4-upper leaves in 18 squash cultivar were the highest tolerance to the paraquat application, followed by third, the second, and the first leaves (older leaves). The forth leaves in Joongangaehobak showed more than three times higher tolerance to the paraquat application than did the first leaves. When the combining of water extract from the fourth leaves with paraquat were applied to the leaves and stems of maize, the paraquat phytotoxicity in maize was reduced compared to the paraquat application alone. Therefore, this study continued to investigate if the phytotoxicity inhibitor exist in the fourth leaves. The water extract in the fourth leaves were isolated by silica gel column chromatography, Sephadex LH-20 column chromatography, TLC, and HPLC, and the substance in the extract was speculated as a malic acid by identifying through NMR. The mixture malic acid and paraquat were applied to the maize to verify the application effect of malic acid on paraquat toxicity. The 100 ${\mu}M$ of paraquat application alone showed 62% of paraquat toxicity to the corn leaves, while the combined application of 100 ${\mu}M$ paraquat with malic acid at 0.1, 0.3, 0.5, and 1.0% did not show the symptom.