• 통합자연과학논문집
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• 제14권3호
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• pp.99-106
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• 2021

A mixture of alcohol and water is commonly used as antifreeze, liquor, and the fundamental solvents for the manufacture of cosmetics, pharmaceuticals, and inks in our daily life. Since various properties of alcohol water mixtures such as density, boiling or melting point, viscosity, and dielectric constant are determined by their mixing ratio, it is very important to know the mixing ratio to predict their properties. One of simple method to find the mixing ratio is measuring the density of the mixtures. However, it is not easy to predict the mixing ratio from the density of the mixtures because the relationship between mixing ratio and density has not been established well. The relationship is dependent on the relative sizes of solute and solvent molecules, and their interactions. Recently, an empirical model to predict the density of glycerol water mixture from their mixing ratio has been introduced. The suggested model is simple but quite accurate for glycerol water mixture. In this article, we investigated the applicability of this model to different alcohol water mixtures. Densities for six different alcohol water mixtures containing various alcohols (e.g., ethylene glycol, 1,3-propane diol, propylene glycol, methanol, ethanol, and 1-propanol) were simulated and compared to experimentally measured ones to investigate the applicability of the model proposed for glycerol water mixtures to other alcohol water mixtures. The model predicted the actual density of all alcohol water mixtures tested in this article with high accuracy at various ratios. This model can probably be used to predict the mixing ratio of other alcohol water mixtures from their densities beyond 6 alcohols tested in this article from their densities.

• Journal of Microbiology and Biotechnology
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• 제30권12호
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• pp.1905-1911
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• 2020

Homoserine dehydrogenase (HSD) catalyzes the reversible conversion of ʟ-aspartate-4-semialdehyde to ʟ-homoserine in the aspartate pathway for the biosynthesis of lysine, methionine, threonine, and isoleucine. HSD has attracted great attention for medical and industrial purposes due to its recognized application in the development of pesticides and is being utilized in the large scale production of ʟ-lysine. In this study, HSD from Bacillus subtilis (BsHSD) was overexpressed in Escherichia coli and purified to homogeneity for biochemical characterization. We examined the enzymatic activity of BsHSD for ʟ-homoserine oxidation and found that BsHSD exclusively prefers NADP+ to NAD+ and that its activity was maximal at pH 9.0 and in the presence of 0.4 M NaCl. By kinetic analysis, Km values for ʟ-homoserine and NADP+ were found to be 35.08 ± 2.91 mM and 0.39 ± 0.05 mM, respectively, and the Vmax values were 2.72 ± 0.06 μmol/min-1 mg-1 and 2.79 ± 0.11 μmol/min-1 mg-1, respectively. The apparent molecular mass determined with size-exclusion chromatography indicated that BsHSD forms a tetramer, in contrast to the previously reported dimeric HSDs from other organisms. This novel oligomeric assembly can be attributed to the additional C-terminal ACT domain of BsHSD. Thermal denaturation monitoring by circular dichroism spectroscopy was used to determine its melting temperature, which was 54.8℃. The molecular and biochemical features of BsHSD revealed in this study may lay the foundation for future studies on amino acid metabolism and its application for industrial and medical purposes.

• 대한심미치과학회지
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• 제6권1호
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• pp.18-31
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• 1997

By the concerns of esthetic restoration were increased recently. many all ceramic crowns were developed. But they except In-Ceram Alumina were used only single crown. In-Ceram Alumina, developed by Dr. Sadon, was revealed to have high flexural Strength(450MPa). So it could be used not only anterior bridges but also posterior bridges. But In-Ceram Alumina was seen to be opaque, a little green color in transillumination light by high content of alumina oxide(85%). So new all ceramics with high strength and high translucence were needed. Spinell($MgAl_2O_3$) have a high melting point, high flexural strength, low heat conductivity, high light conductivity. In-Ceram Spinell offers glasslike light transmission by using the spinell cores instead of the alumina cores. And they have a high translucency like to natural tooth, an excellent margin integrity and a high strength(350MPa). The purposes of this study are 1) to know about the construction method of In-Ceram Spinell System, 2) to investigate the its clinical possibiliy through patients and literature reviews.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.562-567
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• 1999

Single-stranded DNA binding protein (SSB) is well-characterized as having a helix-destabilizing activity. The helix-destabilizing capability of SSB has been re-examined in this study. The results of restriction endonuclease protection assays and titration experiments suggest that the stimulatory effect of SSB on strand exchange acts by melting out the secondary structure which is inaccessible to RecA protein binding; however, SSB is excluded from regions of secondary structure present in native single-stranded DNA. Complexes of SSB and RecA protein are required for eliminating the secondary structure barriers under optimal conditions for strand exchange.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2022년도 봄 학술논문 발표대회
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• pp.115-116
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• 2022

This study incorporates fine waste glass (GS) as a replacement for natural sand (NS) in fly ash (FA) based alkali activated mortar (AAm). AAms were heated at elevated temperature of 200℃, 400℃, 600℃, and 800℃ to explore the residual mass, compressive strength, thermal expansion and change in microstructure of matrix. Results showed greater resistance of AAms with increasing GS content to 50% at each temperature. Owing to the melting of GS at 800℃, the greater matrix bond was observed for AAm incorporating 75% and 100% GS as a result, the residual compressive strength was increased.

• 한국산학기술학회논문지
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• 제2권2호
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• pp.7-10
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• 2001

보석이 포함된 장신구는 보석을 고정시키고 최대한 보석의 심미적 기능을 살리기 위해 후면부로부터 금속 난발(prongs)을 사용한다. 최근의 주얼리 산업은 빠른 유행주기 변화에 따른 신속한 개발을 위해 귀금속이 가능한한 적게 들어간 단발난집형의 목걸이 귀걸이류가 필요하다. 본 연구는 단발난집에 보석을 세팅하여 가볍고 안정적인 신개념의 목걸이를 개발하기 위하여 (주)아메스 개발부와 함께 단발난집 공정을 연구하였다. 보석의 측면부분에서 상측으로 중력방향에 대하여 직교하도록 트렌치를 가공하여 금속틀의 프롱을 접촉시키고 저융점을 갖는 소정의 Sn계솔더를 채용하여 트렌치부에 흡침된 솔더를 응고하여 접합을 완성하였다. 실시예로서 완성된 천연 자수정 스톤과 18K Au님의 단발난집에 적용한 결과 기존 제품에 비해 40% 이상의 Au 무게감소에 따른 비용절감과 우수한 착용감의 새로운 펜던트형 장신구의 개발이 가능하였다.

• Applied Biological Chemistry
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• 제34권4호
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• pp.379-385
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• 1991

12품종의 쌀로 부터 분리 정제한 전분의 2.5% (w/v) 현탁액을 각각 $65^{\circ}C$에서 $95^{\circ}C$까지 $5^{\circ}C$ 간격으로 30분간 가열 조리한 후, 전분 입자들의 형태학적 변화를 주사현미경으로 관찰 하였다. 품종에 관계없이 쌀 전분 입자들은 온도가 증가함에 따라 점진적인 형태학적 변화를 보여주었다. 이들 형태학적 변화는 전분의 팽윤도, 용해도 및 아밀로그라프점성 특성등과 밀접한 연관성을 보였다. 전분 입자로 부터 가용성 물질이 용출되는 것과 팽윤된 전분 입자가 그 자체의 독특한 형태를 잃고 서로 망상구조로 연결되면서 이들 리올로지 특성들의 급격한 증가를 가져왔다. 초기 팽윤 단계에서 전분 입자들의 독특한 구조손실 시기는 각각의 호화온도에 영향을 받았다. 호화 온도 이상의 높은 온도에서 전분 입자들이 삼차원적 망상구조를 형성하는 정도는 아밀로오스 함량이 감소할 수록 망상구조가 더 세밀해지며, 증가하는 경향을 보였다.

• 한국농공학회논문집
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• 제63권3호
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• pp.87-95
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• 2021

The Doam Lake Watershed is one of Gangwon-do's non-point source management areas. This area has a lot of snowfall in winter, and it is expected that there will be a lot of soil erosion in early spring due to snow melting. In this study, snow melting was monitored in the Doam Lake watershed from February to 3, 2020. It was conducted to analyze the water quality changes by calculating the concentration of non-point source pollution caused by snowmelt, and to compare the concentration of water quality during snowmelt event with rainfall and non-rainfall event. As a result of water quality analysis, Event Mean Concentration (EMC) at the first monitoring was SS 33.9 mg/L, TP 0.13 mg/L, TN 4.33 mg/L, BOD 1.35 mg/L, TOC 1.84 mg/L. At the second monitoring, EMC were SS 81.3 mg/L, TP 0.15 mg/L, TN 3.12 mg/L, BOD 1.32 mg/L, TOC 3.46 mg/L. In parameter except SS, it showed good water quality. It is necessary to establish management measures through continuous monitoring.

• Bulletin of the Korean Chemical Society
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• 제33권7호
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• pp.2213-2218
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• 2012

Efficient synthetic routes were developed to prepare a sizable amount (4-15 grams) of the chiral epoxides 4-6 as versatile intermediates for the synthesis of aspartyl protease inhibitors of therapeutic interest such as HIV protease and ${\beta}$-secretase. Oxidative cleavage of the C(2)-C(3) double bond of L-ascorbic acid followed by functional group manipulation led to the preparation of the epoxide 10, which was opened with an azide to yield a common aziridine intermediate 12. Through opening of the aziridine ring of 12 with either a carbon or a sulfur nucleophile, chiral epoxide precursors 4-6 could be prepared for various HIV protease inhibitors. Except for the final low melting epoxides 5 and 6, all intermediates were obtained as crystalline solids, thus the synthetic pathway can be easily applied to a large-scale synthesis of the chiral epoxides.

• Korean Chemical Engineering Research
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• 제47권3호
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• pp.303-309
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• 2009

폐 농업용 비닐을 이용한 연료유 생산 공정을 위한 저밀도폴리에틸렌(LDPE)과 에틸렌비닐아세테이트(EVA) 수지에 대한 열분해 반응 실험을 하였다. 질소 분위기에서 상온에서 $650^{\circ}C$까지의 비등온 조건에서의 열분석기(열중량분석기, 시차주사열량계)와 $420^{\circ}C$의 배치형 반응기에서 무촉매 반응과 천연제올라이트, FCC 촉매, 폐 FCC 촉매, 중국 촉매(촉매 A) 등의 제올라이트계 촉매를 사용한 열분해가 행하여졌다. TGA 실험에서 무적제가 첨가된 EVA 수지는 열분해 개시 온도가 $250^{\circ}C$ 근처로 매우 낮아졌으나, 황토와 장수제 첨가는 열분해를 다소 지연시켰다. LDPE에서 제올라이트 계열 촉매는 촉매 A>폐 FCC 촉매>천연제올라이트>LDPE의 순으로 열분해 반응속도를 높이는데 유효하였다. EVA에서 제올라이트 계열 촉매 첨가 시는 폐 FCC 촉매>천연제올라이트>촉매 A>EVA 수지의 순으로 열분해 반응을 촉진시켰다. DSC 실험에서 제올라이트 계열 촉매 첨가 시 촉매 A>폐 FCC 촉매>천연제올라이트>LDPE의 순으로 융해개시 온도와 열분해열이 낮아졌다. 회분식 실험에서 천연제올라이트 첨가 시 시료 중 가장 높은 액상의 연료유 생성수율을 얻었다.