• Toxicological Research
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• v.29 no.1
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• pp.35-42
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• 2013

Quantum dots (QDs) have received considerable attention due to their potential role in photosensitization during photodynamic therapy. Although QDS are attractive nanomaterials due to their novel and unique physicochemical properties, concerns about their toxicity remain. We suggest a combination strategy, CdSe/ZnS QDs together with curcumin, a natural yellow pigment from turmeric, to reduce QD-induced cytotoxicity. The aim of this study was to explore a potentially effective cancer treatment: co-exposure of HL-60 cells and human normal lymphocytes to CdSe/ZnS QDs and curcumin. Cell viability, apoptosis, reactive oxygen species (ROS) generation, and DNA damage induced by QDs and/or curcumin with or without ultraviolet A (UVA) irradiation were evaluated in both HL-60 cells and normal lymphocytes. In HL-60 cells, cell death, apoptosis, ROS generation, and single/double DNA strand breaks induced by QDs were enhanced by treatment with curcumin and UVA irradiation. The protective effects of curcumin on cell viability, apoptosis, and ROS generation were observed in normal lymphocytes, but not leukemia cells. These results demonstrated that treatment with QD combined with curcumin increased cell death in HL-60 cells, which was mediated by ROS generation. However, curcumin acted as an antioxidant in cultured human normal lymphocytes.

• Nuclear Engineering and Technology
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• v.27 no.5
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• pp.743-752
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• 1995

This paper investigates the effects of fuel groupings in the coupled internals and core model on the internals and fuel responses due to pipe breaks. The 177 fuel assemblies for Korean standard nuclear power plant are grouped into several stick models and the responses of internals components are calculated. The analysis results show that the fuel model groupings in the coupled internals and core model have no significant effects on the internals and fuel responses for pipe break excitation. Also, in order to determine the feasibility of constructing a single equivalent stick representation of In or more adjacent fuel bundles, the reduced models, each of which employs a different stiffness lumping rule, are constructed. It is shown that the equivalent stiffness calculated to get the first natural frequency of the original model while preserving net gap between grouping centers gives the minimum modelling error.

• The Korean Journal of Physiology and Pharmacology
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• v.13 no.5
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• pp.343-348
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• 2009

53BP1 is an important genome stability regulator, which protects cells against double-strand breaks. Following DNA damage, 53BP1 is rapidly recruited to sites of DNA breakage, along with other DNA damage response proteins, including ${\gamma}$-H2AX, MDC1, and BRCA1. The recruitment of 53BP1 requires a tandem Tudor fold which associates with methylated histones H3 and H4. It has already been determined that the majority of DNA damage response proteins are phosphorylated by ATM and/or ATR after DNA damage, and then recruited to the break sites. 53BP1 is also phosphorylated at several sites, like other proteins after DNA damage, but this phosphorylation is not critically relevant to recruitment or repair processes. In this study, we evaluated the functions of phosphor-53BP1 and the role of the BRCT domain of 53BP1 in DNA repair. From our data, we were able to detect differences in the phosphorylation patterns in Ser25 and Ser1778 of 53BP1 after neocarzinostatin-induced DNA damage. Furthermore, the foci formation patterns in both phosphorylation sites of 53BP1 also evidenced sizeable differences following DNA damage. From our results, we concluded that each phosphoryaltion site of 53BP1 performs different roles, and Ser1778 is more important than Ser25 in the process of DNA repair.

• The Journal of the Acoustical Society of Korea
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• v.16 no.1
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• pp.24-32
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• 1997

This study aims to describe, at one aspect, the relativity between syntactic structure and prosodic phrasing, and at the other, to establish a suitable phrasing pattern to produce more natural synthetic speech. To get meaningful results, all the word boundaries in the prosodic database were statistically analyzed, and assigned by the proper boundary type. The resulting 10 types of prosodic boundaries were classified into 3 types according to the strength of the breaks, which are zero, minor, and major break respectively. We have found out that the durational information was a main cue to determine the major prosodic boundary. Using the bigram and trigram of syntactic information, we predicted major and minor classification of boundary types. With brigram model, we obtained the correct major break prediction rates of 4.60%, 38.2%, the insertion error rates of 22.8%, 8.4% on each Test-I and Test-II text database respectively. With trigram mode, we also obtained the correct major break prediction rates of 58.3%, 42.8%, the insertion error rates of 30.8%, 42.8%, the insertion error rates of 30.8%, 11.8% on Test-I and Test-II text database respectively.

• Journal of Microbiology
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• v.37 no.4
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• pp.248-255
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• 1999

We have analyzed the MRE3/REC114 gene of Saccharomyces cerevisiae, previously detected in isolation of mutants defective in meiotic recombination. We cloned the MRE3/REC114 gene by complementation of the meiotic recombination defect and it has been mapped to chormosome XIII. The DNA sequence analysis revealed that the MRE3 gene is identical to the REC114 gene. The upstream region of the MRE3/REC114 gene contains a T_4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. Coincidentally, northern blot analysis indicated that the three sizes of MRE3/REC114 transcripts, 3.4, 1.4 and 1.2 kb, are induced in meiosis. A less abundant transcript of 1.4 kb is detected in both mitotic and meiotic cells, suggesting that it is needed in mitosis as well as meiosis. To examine the role of the MRE3/REC114 gene, we constructed mre3 disruption mutants. Strains carrying an insertion or null deletion of the MRE3/REC114 gene showed slow growth in nutrient medium and the doubling time of these cells increased approximately by 2-fond compared to the wild-type strain. Moreover, the deletion mutant (${\delta}$mre3) displayed no meiotically induced recombination and no viable spores. The mre3/rec114 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass reductional division. The double-stranded breaks (DSBs) which are involved in initiation of meiotic recombination were not detected in the analysis of meiotic chromosomal DNA from the mre3/rec114 disruptant. From these results we suggest that the MRE3/REC114 gene product is essential in normal growth and in early meiotic stages involved in meiotic recombination.

• International Journal of Internet, Broadcasting and Communication
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• v.15 no.1
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• pp.126-139
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• 2023

At present, naked-eye 3D appears more and more commonly on the facades of urban buildings. It brings an incredible visual experience to the audience by simulating the natural 3D 3D space effect. At the same time, it also creates enormous commercial value for city publicity and commercial advertisements. There is much research on naked-eye 3D visual effects, but for right-angle LED screens. Right-angle LED screen's brand-new expression method that has only become popular in recent years, how to convey a realistic naked-eye 3D effect through two LED screens combined at right angles has become a problem worth exploring. To explore the whole design ideas and production process of the naked-eye 3D impact of the right-angle LED screen, this paper is a preliminary study aimed at understanding the performance principle and expression features. Before the case analysis, first, understand the standard virtual 3D space construction techniques. Combining it with the optical illusion phenomenon, according to the expression principle of the naked-eye 3D effect of the right-angle LED screen, it can be summarized into seven expressions: Shadow, Color contrast, Background structure line, Magnify object, Object out of bounds, Object floating, Fusion of picture and background. By analyzing the optical illusion phenomenon used in the case, we summarized the main performance characteristics of the naked eye 3D effect. The emergence of right-angle LED screens breaks the limitation of a single plane of optical illusion art, perfectly combines building facades with naked-eye 3D visual effects, and provides designers with a brand-new creative platform. Understanding its production principles and main expressive features can help designers enter this innovative platform better.

• Journal of The Korean Society of Agricultural Engineers
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• v.64 no.3
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• pp.63-73
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• 2022

A more accurate understanding of the irrigation water supply is necessary for efficient agricultural water management. Although we measure water levels in an irrigation canal using ultrasonic water level gauges, some errors occur due to malfunctions or the surrounding environment. This study aims to apply CNN (Convolutional Neural Network) Deep-learning-based image classification and segmentation models to the irrigation canal's CCTV (Closed-Circuit Television) images. The CCTV images were acquired from the irrigation canal of the agricultural reservoir in Cheorwon-gun, Gangwon-do. We used the ResNet-50 model for the image classification model and the U-Net model for the image segmentation model. Using the Natural Breaks algorithm, we divided water level data into 2, 4, and 8 groups for image classification models. The classification models of 2, 4, and 8 groups showed the accuracy of 1.000, 0.987, and 0.634, respectively. The image segmentation model showed a Dice score of 0.998 and predicted water levels showed R² of 0.97 and MAE (Mean Absolute Error) of 0.02 m. The image classification models can be applied to the automatic gate-controller at four divisions of water levels. Also, the image segmentation model results can be applied to the alternative measurement for ultrasonic water gauges. We expect that the results of this study can provide a more scientific and efficient approach for agricultural water management.

• The Journal of the Convergence on Culture Technology
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• v.8 no.3
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• pp.493-499
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• 2022

This paper reports the results of the eruption of a volcano on Jeju Island at a certain rate, and the tumulus formed after the eruption and the basalt that erupted from the middle of Mt. Halla washed up to the sea. We analyzed the speed when basalt underground magma breaks through the neutral zone on the ground with an absolute temperature of about 1000K and explodes at an absolute temperature of 1200K at an altitude of 1950m. The density of combustion gas becomes smaller than the surrounding air due to the plume volcanic eruption, which is the heat flow of the flame column due to buoyancy, and buoyancy is generated and an updraft is formed. Flame pillars are classified as continuous, intermittent, and buoyant flame zones. As the speed of the flame pillar of Mt. Halla (1950m) falls from the highest point it has risen, potential energy is converted into kinetic energy and is caused by the flow of fluid, solving these two equations equal, the volcanic eruption velocity is 87.5 m/s. At this time, the density of magma is inversely proportional to the temperature. Geomunoreum (456m) had an explosion speed of 42.6m/s.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.5A
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• pp.551-555
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• 2009

This study is performed to apply a standard to evaluate fire protection assessment for tunnel structures when a fire breaks out in the road tunnel. Recently, a number of road tunnels have been rapidly increased and fire risk also multiplyed according to extend tunnel length, due to natural features and environmentally-friendly road construction in Korea. But we have not yet been prescribed appropriate time-temperature curve for tunnel fire. Therefore, we presented fire design model and investigated time-temperature curve proposed by a foreign country considering traffic, a kinds of vehicles which are a basis of heat rate. At the end, Hydrocarbon modified curve applied as design fire model by using numerical analysis and presented design fire model and examined the effects of tunnel structures.

• ALGAE
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• v.38 no.4
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• pp.295-306
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• 2023

Human coronavirus diseases, particularly severe acute respiratory syndrome coronavirus 2, still remain a persistent public health issue, and many recent studies are focusing on the quest for new leads against coronaviruses. To contribute to this growing pool of knowledge and explore the available marine natural products against coronaviruses, this study investigated the antiviral effects of fucoxanthin isolated from Sargassum siliquastrum-a brown alga found on Jeju Island, South Korea. The antiviral effects of fucoxanthin were confirmed in severe acute respiratory syndrome coronavirus 2-infected Vero cells, and its structural characteristics were verified in silico using molecular docking and molecular dynamic simulations and in vitro colorimetric method. Fucoxanthin inhibited the infection in a concentration-dependent manner, without showing cytotoxicity. Molecular docking simulations revealed that fucoxanthin binds to the angiotensinconverting enzyme 2-spike protein (binding energy -318.306 kcal mol^-1) and main protease (binding energy -205.118 kcal mol^-1). Moreover, molecular dynamic simulations showed that fucoxanthin remains docked to angiotensin-converting enzyme 2-spike protein for 20 ns, whereas it breaks away from main protease after 3 ns. Also, the in silico prediction of the fucoxanthin was verified through the in vitro colorimetric method by inhibiting the binding between angiotensinconverting enzyme 2 and spike protein in a concentration-dependent manner. These results indicate that fucoxanthin exhibits antiviral effects against severe acute respiratory syndrome coronavirus 2 by blocking the entry of the virus. Therefore, fucoxanthin from S. siliquastrum can be a potential candidate for treating coronavirus infection.