• Toxicological Research
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• 제29권1호
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• pp.35-42
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• 2013

Quantum dots (QDs) have received considerable attention due to their potential role in photosensitization during photodynamic therapy. Although QDS are attractive nanomaterials due to their novel and unique physicochemical properties, concerns about their toxicity remain. We suggest a combination strategy, CdSe/ZnS QDs together with curcumin, a natural yellow pigment from turmeric, to reduce QD-induced cytotoxicity. The aim of this study was to explore a potentially effective cancer treatment: co-exposure of HL-60 cells and human normal lymphocytes to CdSe/ZnS QDs and curcumin. Cell viability, apoptosis, reactive oxygen species (ROS) generation, and DNA damage induced by QDs and/or curcumin with or without ultraviolet A (UVA) irradiation were evaluated in both HL-60 cells and normal lymphocytes. In HL-60 cells, cell death, apoptosis, ROS generation, and single/double DNA strand breaks induced by QDs were enhanced by treatment with curcumin and UVA irradiation. The protective effects of curcumin on cell viability, apoptosis, and ROS generation were observed in normal lymphocytes, but not leukemia cells. These results demonstrated that treatment with QD combined with curcumin increased cell death in HL-60 cells, which was mediated by ROS generation. However, curcumin acted as an antioxidant in cultured human normal lymphocytes.

• Nuclear Engineering and Technology
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• 제27권5호
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• pp.743-752
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• 1995

본 논문은 배관파단에 대한 원자로 내부구조물의 해석시 사용되는 원자로 내부구조물과 노심의 커플(couple)된 모델에서 핵연료집합체의 grouping수에 따른 동적 응답의 영향을 조사한 것이다. 177개의 핵연료집합체를 1, 3, 5 그리고 7개의 그룹으로 나누어 모델링 하였고 그 각각에 대한 응답을 구하였다. 해석결과 원자로 내부구조물과 핵연료집합체의 배관파단에 대한 응답은 핵연료집합체의 grouping수에 거의 영향을 받지 않음을 알 수 있었다. 또한 핵연료집합체의 해석시 사용되는 상세모델에서 2개 이상의 이웃하는 핵 연료다발을 하나의 등가모델로 나타내는 방법을 연구한 결과 집합체의 1차모드 주파수와 일치하는 등가스프링을 사용하고 각 핵연료다발사이의 간격을 그대로 유지했을 때의 모델이 원래의 응답과 가장 잘 일치함을 보였다.

• The Korean Journal of Physiology and Pharmacology
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• 제13권5호
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• pp.343-348
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• 2009

53BP1 is an important genome stability regulator, which protects cells against double-strand breaks. Following DNA damage, 53BP1 is rapidly recruited to sites of DNA breakage, along with other DNA damage response proteins, including ${\gamma}$-H2AX, MDC1, and BRCA1. The recruitment of 53BP1 requires a tandem Tudor fold which associates with methylated histones H3 and H4. It has already been determined that the majority of DNA damage response proteins are phosphorylated by ATM and/or ATR after DNA damage, and then recruited to the break sites. 53BP1 is also phosphorylated at several sites, like other proteins after DNA damage, but this phosphorylation is not critically relevant to recruitment or repair processes. In this study, we evaluated the functions of phosphor-53BP1 and the role of the BRCT domain of 53BP1 in DNA repair. From our data, we were able to detect differences in the phosphorylation patterns in Ser25 and Ser1778 of 53BP1 after neocarzinostatin-induced DNA damage. Furthermore, the foci formation patterns in both phosphorylation sites of 53BP1 also evidenced sizeable differences following DNA damage. From our results, we concluded that each phosphoryaltion site of 53BP1 performs different roles, and Ser1778 is more important than Ser25 in the process of DNA repair.

• 한국음향학회지
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• 제16권1호
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• pp.24-32
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• 1997

이 논문에서는 첫 번째로 운율구 경계를 자동으로 추출하고자 할 때 인간의 지각과 관련하여 어떠한 자질을 이용하는 것이 좋은가 하는 점을 밝혀 보았다. 운율구 경계의 유형은 크게 경계가 없는 강도(zero), 약한 경계 강도(minor break strength), 강한 경계 강도(major break strength) 3 단계로 정하는 것이 통계적으로 의의가 있으며 지속시간의 정보가 강한 경계 유형의 결정에 중요하게 작용하는 자질이었음을 알 수 있었다. 두 번째로는 운율구 경계현상 분석결과를 바탕으로 운율구 경계의 경계 강도를 약한 경계 강도(zero를 포함)와 강한 경계 강도로 나누고, 2단계의 경계 강도를 텍스트상에서 문장성분의 bigram, trigram을 정보를 이용하여 자동으로 할당하였다. Bigram의 경우 Test-I, Test-II 텍스트 DB에 대해 각각 46.0%, 38.2%의 강한 경계 강도 예측정확률과 22.8%, 8.4%의 삽입오류율의 성능을 내었으며, Trigram인 경우 Test-I, Test-II 텍스트 DB 각각에 대해 58.3%, 42.8%의 강한 경계 강도 예측정확률과 30.0%, 11.8%의 삽입오류율를 나타냈다.

• Journal of Microbiology
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• 제37권4호
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• pp.248-255
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• 1999

We have analyzed the MRE3/REC114 gene of Saccharomyces cerevisiae, previously detected in isolation of mutants defective in meiotic recombination. We cloned the MRE3/REC114 gene by complementation of the meiotic recombination defect and it has been mapped to chormosome XIII. The DNA sequence analysis revealed that the MRE3 gene is identical to the REC114 gene. The upstream region of the MRE3/REC114 gene contains a T_4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. Coincidentally, northern blot analysis indicated that the three sizes of MRE3/REC114 transcripts, 3.4, 1.4 and 1.2 kb, are induced in meiosis. A less abundant transcript of 1.4 kb is detected in both mitotic and meiotic cells, suggesting that it is needed in mitosis as well as meiosis. To examine the role of the MRE3/REC114 gene, we constructed mre3 disruption mutants. Strains carrying an insertion or null deletion of the MRE3/REC114 gene showed slow growth in nutrient medium and the doubling time of these cells increased approximately by 2-fond compared to the wild-type strain. Moreover, the deletion mutant (${\delta}$mre3) displayed no meiotically induced recombination and no viable spores. The mre3/rec114 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass reductional division. The double-stranded breaks (DSBs) which are involved in initiation of meiotic recombination were not detected in the analysis of meiotic chromosomal DNA from the mre3/rec114 disruptant. From these results we suggest that the MRE3/REC114 gene product is essential in normal growth and in early meiotic stages involved in meiotic recombination.

• International Journal of Internet, Broadcasting and Communication
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• 제15권1호
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• pp.126-139
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• 2023

At present, naked-eye 3D appears more and more commonly on the facades of urban buildings. It brings an incredible visual experience to the audience by simulating the natural 3D 3D space effect. At the same time, it also creates enormous commercial value for city publicity and commercial advertisements. There is much research on naked-eye 3D visual effects, but for right-angle LED screens. Right-angle LED screen's brand-new expression method that has only become popular in recent years, how to convey a realistic naked-eye 3D effect through two LED screens combined at right angles has become a problem worth exploring. To explore the whole design ideas and production process of the naked-eye 3D impact of the right-angle LED screen, this paper is a preliminary study aimed at understanding the performance principle and expression features. Before the case analysis, first, understand the standard virtual 3D space construction techniques. Combining it with the optical illusion phenomenon, according to the expression principle of the naked-eye 3D effect of the right-angle LED screen, it can be summarized into seven expressions: Shadow, Color contrast, Background structure line, Magnify object, Object out of bounds, Object floating, Fusion of picture and background. By analyzing the optical illusion phenomenon used in the case, we summarized the main performance characteristics of the naked eye 3D effect. The emergence of right-angle LED screens breaks the limitation of a single plane of optical illusion art, perfectly combines building facades with naked-eye 3D visual effects, and provides designers with a brand-new creative platform. Understanding its production principles and main expressive features can help designers enter this innovative platform better.

• 한국농공학회논문집
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• 제64권3호
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• pp.63-73
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• 2022

A more accurate understanding of the irrigation water supply is necessary for efficient agricultural water management. Although we measure water levels in an irrigation canal using ultrasonic water level gauges, some errors occur due to malfunctions or the surrounding environment. This study aims to apply CNN (Convolutional Neural Network) Deep-learning-based image classification and segmentation models to the irrigation canal's CCTV (Closed-Circuit Television) images. The CCTV images were acquired from the irrigation canal of the agricultural reservoir in Cheorwon-gun, Gangwon-do. We used the ResNet-50 model for the image classification model and the U-Net model for the image segmentation model. Using the Natural Breaks algorithm, we divided water level data into 2, 4, and 8 groups for image classification models. The classification models of 2, 4, and 8 groups showed the accuracy of 1.000, 0.987, and 0.634, respectively. The image segmentation model showed a Dice score of 0.998 and predicted water levels showed R² of 0.97 and MAE (Mean Absolute Error) of 0.02 m. The image classification models can be applied to the automatic gate-controller at four divisions of water levels. Also, the image segmentation model results can be applied to the alternative measurement for ultrasonic water gauges. We expect that the results of this study can provide a more scientific and efficient approach for agricultural water management.

• 문화기술의 융합
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• 제8권3호
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• pp.493-499
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• 2022

본 논문은 제주도의 화산이 폭발 시에 얼마만한 속도로 폭발했으며, 폭발 후에 생기는 튜물러스와 한라산 중턱에서 폭발된 현무암이 바다까지 밀려온 결과를 보고한다. 현무암 지하 마그마가 약 절대온도 1000K로 지상의 중성대를 뚫고 1950m 상공에서 절대온도 1200K로 폭발하면서 나올 때의 속도에 대해 분석했다. 부력(浮力)에 의한 화염 기둥의 열기류인 Plume 화산 폭발로 인한 주변의 공기보다 연소가스의 밀도가 작아져 부력이 발생하고 상승 기류가 형성된다. 화염 기둥은 연속, 간헐, 부력 화염 영역으로 분류된다. 한라산(1950m) 화염 기둥의 속도는 상승한 최고점에서 낙하함으로 위치에너지가 운동에너지로 변환되어 유체흐름에 의해 이 두 식을 같게 놓아 풀면 화산 폭발 속도87.5m/s를 얻었다. 이때 마그마의 밀도는 온도에 반비례한다. 거문오름(456m)은 폭발 속도는 42.6m/s로 계산했다.

• 대한토목학회논문집
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• 제29권5A호
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• pp.551-555
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• 2009

본 연구는 도로터널 내 화재시 콘크리트 구조체의 내화성능을 평가하기 위한 기준을 제시하고자 실시하였다. 현재 국내에서는 지형특성 및 환경친화적인 도로건설로 도로 터널의 수가 빠른 속도로 증가하며, 터널연장이 길어짐에 따라 터널 내 화재사고가 갈수록 높아지고 있는 상황이다. 하지만 우리나라에서는 터널 화재에 대한 적합한 시간-온도 곡선을 규정하지 못하고 있는 실정이다. 따라서 본 연구에서는 국내 도로의 통행량, 차량 종류등을 고려한 열방출율을 기초로 외국에서 제시된 시간-온도 곡선을 검토해 보았으며 적합한 설계화재 모델을 적용하였다. 또한 적용된 도로터널의 설계화재모델에 따른 수치해석을 통하여 터널 구조체의 화재시 성능을 검토하였다.

• ALGAE
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• 제38권4호
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• pp.295-306
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• 2023

Human coronavirus diseases, particularly severe acute respiratory syndrome coronavirus 2, still remain a persistent public health issue, and many recent studies are focusing on the quest for new leads against coronaviruses. To contribute to this growing pool of knowledge and explore the available marine natural products against coronaviruses, this study investigated the antiviral effects of fucoxanthin isolated from Sargassum siliquastrum-a brown alga found on Jeju Island, South Korea. The antiviral effects of fucoxanthin were confirmed in severe acute respiratory syndrome coronavirus 2-infected Vero cells, and its structural characteristics were verified in silico using molecular docking and molecular dynamic simulations and in vitro colorimetric method. Fucoxanthin inhibited the infection in a concentration-dependent manner, without showing cytotoxicity. Molecular docking simulations revealed that fucoxanthin binds to the angiotensinconverting enzyme 2-spike protein (binding energy -318.306 kcal mol^-1) and main protease (binding energy -205.118 kcal mol^-1). Moreover, molecular dynamic simulations showed that fucoxanthin remains docked to angiotensin-converting enzyme 2-spike protein for 20 ns, whereas it breaks away from main protease after 3 ns. Also, the in silico prediction of the fucoxanthin was verified through the in vitro colorimetric method by inhibiting the binding between angiotensinconverting enzyme 2 and spike protein in a concentration-dependent manner. These results indicate that fucoxanthin exhibits antiviral effects against severe acute respiratory syndrome coronavirus 2 by blocking the entry of the virus. Therefore, fucoxanthin from S. siliquastrum can be a potential candidate for treating coronavirus infection.