• Bulletin of the Korean Chemical Society
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• v.35 no.10
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• pp.3015-3020
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• 2014

The behavior of benzo[a]pyrene-7,8-dione (BPQ) in aqueous solution and its interaction with native DNA was investigated using conventional absorption and linear dichroism (LD) spectroscopy. The appearance of a broad absorption maximum at long wavelengths and its proportional relationship to solvent polarizability suggested that BPQ adopts a aggregated state for all solutions examined. Disappearance of this absorption band at higher temperatures in aqueous solution also supported BPQ aggregation. When associated with DNA absorption spectral properties were essentially the same as that in aqueous solution. However, two isosbestic wavelengths were found in the concentration-dependent absorption spectrum of the BPQ-DNA complex, suggesting the presence of at least two or more DNA-bound BPQ species. Both species produced $LD^r$ spectra whose magnitude in BPQ absorption region is larger or comparable to that in the DNA absorption region, suggesting that the molecular BPQ plane is near perpendicular relative to the local DNA helical axis. Therefore, BPQ molecules are aligned along the DNA stem in both DNA-aggregated BPQ species.

• Korean Journal of Plant Resources
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• v.10 no.4
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• pp.334-345
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• 1997

This research was conducted to determine contents of saponins and alkloids for plants distributed in the sourthern region of Korea. The extracts from each plants were purified by solvent fractionation, column chromatography, TLC and analyzed GC and HPLC. As a result, contents crude gingseng saponins and saikosaponins were the highest in Oenothera odorata and Metaplexis japonica, respectively. and contents crude alkaloids was the highest in Metaplexis japonica among the all plants xamined. HPLC was conducted to detect of saponins. As a result, ginseng saponin-like substances was detected in the extracts of Euphobia splendens, Taraxacum mongolicum and Metaplexis japonica, gingsen-like substances and its of saikosaponin c-like substances was detected in the extracts of Camellia japonica and Aleurites fordii. GC was conducted to detect of alkaloid. As a result, nicotine-like substances was detected in only the extracts of Fatsia japonica.

• Korean Journal of Plant Resources
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• v.10 no.4
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• pp.324-333
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• 1997

This research was conducted to determine contents of lipids and hydrocarbons for plants distributed in the sourthern region of Korea. The lipid extracts from each plants were purified by solvent fractionation, column chromatography. TLC and analyzed by gas chromatography. As a result, contents netural lipids and phospholipids were the highest in Aleurites fordii and Camellia japonica among the all plants examined. Most of the fatty acids in lipids was palmitic acid, oleic acid and linoleic acid. Content of hydrocarbon was the highest in Fatsia japonica among the all plants examined. Hydrocarbons contained in the plant was mainly more than 16 number of carbons.

• BMB Reports
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• v.33 no.5
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• pp.366-369
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• 2000

The nucleotide effects of Hafnia alvei aspartase were investigated. Purine nucleosides, such as adenosine and guanosine, increased the aspartase activities; whereas, purine nucleotides, such as AMP, ATP, GTP and IMP, caused little change in the aspartase activities. However, pyrimidine derivatives, such as cytidine and CTP, decreased the aspartase activity. The nucleotide and nucleoside effects by the limited trypsin-treated aspartase were similar to those of a native enzyme. These results indicate that the COOH-terminal region and an allosteric site might be located away from each other. The initial velocity study in the presence of adenosine showed that $K_m$ for aspartate was decreased to one-sixth of that in the absence of adenosine, but $V_{max}$ was unchanged. The significance of the distinct allosteric effect for the enzyme-nucleotide interaction is discussed.

• Journal of Microbiology and Biotechnology
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• v.8 no.2
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• pp.141-145
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• 1998

Overproduction of intracellular ${\beta}$-glucosidase was attempted by modifying the promoter region of a ${\beta}$-glucosidase gene cloned from Cellulomonas fimi and expressing it in Bacillus subtilis DB 104. A strong engineered promoter, BJ27UΔ88, was fused to the ${\beta}$-glucosidase gene after removing its native promoter. An effective Shine-Dalgamo sequence (genel0 of phage T7) was inserted between the promoter and the ${\beta}$-glucosidase structural gene. The modified gene was overexpressed in B. subtilis and produced 1121.5 units of ${\beta}$-glucosidase per mg protein which is about $12\%$ of total intracellular protein. Secretion of overproduced intracellular ${\beta}$-glucosidase was attempted by using the signal sequence of the Bacillus endoglucanase gene as well as an in-frame hybrid protein of endoglucanase. The hybrid protein was normally secreted into the culture medium and still retained ${\beta}$-glucosidase activity.

• Journal of Korean Society of Rural Planning
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• v.15 no.2
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• pp.59-68
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• 2009

Nowadays, local governments have been developing an image to represent their regions' identity. These characters contain unlimited capabilities of utilization and advertising effect that adds to their high value. However, we must consider how effectively the image helps in representing its region because some characters have been produced upon timely basis without consistent long-term planning. This study is to investigate the image and motif of the local government characters from the consumer's perspective. The results are as follows: 1)The images employed by the local governments are not highly preferred and most regions select motifs from their local agricultural products and native plants. 2)The variables that affect the character of the local governments are such as beauty, identity, etc. In conclusion, in order to maximize the local government image, one must improve the design concepts, encourage advertisement of regional festivities and travel commercialization, and advocate the identity and beauty of the local area to the residents and other regional people.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.28A no.10
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• pp.814-820
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• 1991

TaSi$_2$ was formed by rapid thermal annealing(RTA) on BF$_2$ doped single crystalline silicon substrates. The formation and various properties of TaSi$_2$ have been investigated by using 4-point probe, HP414, XRD, and SEM. And the redistribution of boron with RTA has been observed by SIMS. Implanted boron was diffused out into the TaSi$_2$ for RTA temperature but did not significantly affect the formation temperature of TaSi$_2$. Also, the contact resistance for TaSi$_2$/p$^{+}$ region had a low value 22$\Omega$, at contact size of 0.9$\mu$m, and the native oxide formed on Si-substrates by BF$_2$ implantation retarded the formation of TaSi$_2$.

• Korean Journal of Environment and Ecology
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• v.15 no.2
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• pp.159-172
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• 2001

본 연구는 수도권 지역의 아까시나무림을 중심으로 식물군집의 식생구조적 특성을 분석함으로써 향후, 도시녹지의 자연성 복원을 위한 기초 자료로 제시하고자 하였다. 조사지역은 서울도심지역으로 중구 남산과 서대문구 안산, 서울외곽지역으로 은평구 봉산과 부천시, 성주산, 비도시지역으로 경기도 천마산을 선정하였다. 주요 연구분야는 생태적 특성과 복원모델로 구분하였가. 생태적 특성평가는 천이단계, 자연성 및 다층적 식생구조와 종다양성을 실시하였으며, 복원모델은 적정수종, 개체수, 흉고단면적, 수목간 최단거리를 선정하였다. 조사결과, 복원모델은 비도시지역 중 자연성이 높으며 다층구조를 이루고 있는 자생식물군집을 선정하였으며 적정식물은 교목성장 3종, 아교목성정 7종, 관목성장 16종, 주연부 수종 4종 초본식물 27종이 적절한 것으로 판단되었다. 향후, 아끼시나무림읜 자연성 복원을 위해서 자연림인 참나무류로의 천이를 유도할 수 있도록 자생종 중심의 생태적 복원방안의 모색이 이루어져할 것이다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.10a
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• pp.41-48
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• 1995

To increase the folding efficiency of proinsulin, we have designed a series of mini-proinsulins having the central C-peptide region replaced with sequences forming reverse turns. These proteins were produced as fusion proteins in E. coli in the form of inclusion bodies. After isolation process of the sulfonated mini-proinsulins, the subsequent refolding experiments indicate that the mini-proinsulins, with non-native penta-peptide sequences inserted between two of the enzyme processing sites, show substantially increased folding yields compared with the proinsulin. The correct disulfide connections were verified by fingerprint analysis using Glu-C endoproteinase. These novel mini-proinsulins could be used for the study of folding mechanism of proinsulin.

• Journal of Fashion Business
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• v.15 no.6
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• pp.23-39
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• 2011

Scythian Earrings in the Black Sea region had completely degenerated, stifled by motifs and shapes of Greek origin, retaining its representational realism and its full emotional vitality. The purpose of this study is to review and research the symbolic meaning and classifying the types of the Scythian Earrings style through the tombs bequests. The Scythian Earrings were divided into the styles according to the shape, Earring with Ends Shaped like animal's Heads, Boat-Shaped Earrings, Bird-Shaped Pendants earrings, Earrings with a Disc and a Pendant, Earrings Shaped like the Figures, Earring with the drop Pendant, Spiral Earrings. Earrings based on the boat shape enjoyed a long popularity among the Scythians. As that form became elaborated and combined with the disk-pendent, it reflected native rather than Asian or Hellenic tastes. Although Scythian earrings were produced based on the shapes of Greece earrings, they recreated these as Scythian unique style. In particular, the animal motive and the decoration have various changes. The exquisite earrings attest to the elegant taste and splendid wealth of the upper classes.