• Journal of Life Science
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• v.24 no.9
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• pp.927-934
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• 2014

Sanguinarine, a benzophenanthridine alkaloid originally derived from the root of Sanguinaria canadensis, has been shown to possess antimicrobial, antioxidant, and anti-cancer properties. Although tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is known to induce apoptosis in cancer cells, but not most normal cells and has shown efficacy in a phase 2 clinical trial, development of resistance to TRAIL by tumor cells is a major roadblock. Our previous study indicated that treatment with TRAIL in combination with subtoxic concentrations of sanguinarine sensitized TRAIL-mediated apoptosis in TRAIL-resistant human gastric carcinoma AGS cells; however, the detailed mechanisms are not fully understood. In this study, we show that sanguinarine sensitizes AGS cells to TRAIL-mediated apoptosis as detected by MTT assay, agarose gel electrophoresis, chromatin condensation and flow cytometry analysis. Combined treatment with sanguinarine and TRAIL effectively induced expression of death receptor (DR) 5 but did not affect expression of DR4 and mitogen activated protein kinases signaling molecules. Moreover, the combined treatment with sanguinarine and TRAIL increased the generation of reactive oxygen species (ROS); however, N-acetylcysteine, ROS scavenger, significantly recovered growth inhibition induced by the combined treatment. Taken together, our results indicate that sanguinarine can potentiate TRAIL-mediated apoptosis through upregulation of DR5 expression and ROS generation.

• Journal of Korean Society of Forest Science
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• v.83 no.3
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• pp.286-298
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• 1994

The object of this study was to examine the user's impacts on the environmental deteriorations of trail at Ticket Office - Paekryunsa (Temple) Hyangch$\hat{o}$kpong - Dongyupryung - Chilyun Fall area in T$\hat{o}$kyusan National Park. Four trails were sampled in the study area according to the amount of users. Then the user's impacts on trail were measured at each trail. The Ticket Office-Paekryunsa trail was the most used district and followed at Paekryunsa-Hyangch$\hat{o}$kpong trail, Hyangch$\hat{o}$kpong-Dongyupryung trail in descending order. Dongyupryung-Chilyun Fall trail is not used by people because of rest rotation system. The entire width of trail was greater at the more heavily used trail. Maximum depth, cross-sectional area loss, and surface texture and roughness of trail were the highest at Paekryunsa-Hyangch$\hat{o}$kpong trail. Soil hardness, soil acidity, soil moisture content, organic matter content, and exchange canon were influenced by trampling. Soil hardness, soil acidity and exchange canon increased in tramples soil, but content of soil moisture and organic matter decreased therein. Environmental deteriorations of trail were significantly influenced by the amount of users and the slope of trail. Bared lands about $2.000m^2$ were appeared by trampling and camping around Hyangch$\hat{o}$kpong. Effects of carrying of rest rotation system for National Park were partly recognized at Dongyupryung-Chilyun Fall trail.

• Korean Journal of Environment and Ecology
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• v.12 no.4
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• pp.398-405
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• 1999

This study was carried out to investigate exercise load of visitors and physical condition of stairway in the trail of Keumsan located in Hallyo-Haesang National Park. Total trail length of Keumsan is 2,126m, and the number of step-trail section are 15, and the total length of step-trail section is 1,000.4m. Average gradient of step-trail was 20~40%. But the gradient and length were 45.7% and 268.5m in the section 11, and 58.4% and 188.6m in the section 12. The greatest gradient was 67.8% in the section 13. During the climbing in trail, heart rate were 104~184beats/min.. And in the step-trail section 11, 12, and 13, IHR, ELI, and %HRmax were 166.5%, 89.1%, 92.9%, and 167.4%, 89.6%, 93.2%, and 157.8%, 84.5%, 89.9%, respectively.

• Korean Journal of Veterinary Research
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• v.49 no.1
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• pp.1-8
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• 2009

The ubiquitin-proteasome mediated protein degradation pathway plays an important role in regulating both cell proliferation and cell death. Proteasome inhibitors are well known to induce apoptosis in various human cancer cell lines. We investigated the effect of combined treatment with proteasome inhibitor and TRAIL, and a possible mechanism of the enhancing apoptosis by the both treatment, on TRAIL-resistant non-small cell lung cancer. A549 cells were exposed to the N-Acetyl-Leu-Leu-Norleu-al (ALLN) as a proteasome inhibitor and then treated with recombinant TRAIL protein. In A549 cells under proteasome inhibition conditions by pretreatment with ALLN, TRAIL treatment significantly decreased cell viability compared to that ALLN and TRAIL alone treatment. Also, the both treatment induced cell damage through DNA fragmentation and p53 expression. In addition, the combined treatment of both markedly increased caspase-8 activation, especially the exposure for 2 h, and Bax expression and induced the dissipation of mitochondrial transmembrane potential in A549 cells. Taken together, these findings showed that proteasome inhibition by ALLN enhanced TRAIL-induced apoptosis via DNA degradation by activated P53 and mitochondrial transmembrane potential loss by caspase-8 activation and bax expression. Therefore, our results suggest that proteasome inhibitor may be used a very effectively chemotherapeutic agent for the tumor treatment, especially TRAIL-resistant tumor cell.

• Journal of Korean Society of Rural Planning
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• v.22 no.3
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• pp.21-31
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• 2016

This is a comparative study focused on the scenic landscape preferences derived from visitors's perceptions at the Dulle-gil and the uphill trail in Bukhansan National Park. Unlike previous landscape assessment methods, this study contains more direct and on-site analysis with methods of understanding the visitors' perceptions by using 'visitor employed photography(VEP)' which requires each visitor to take preferable scenic landscape with one's own smart phone camera. The scenic landscape types at two different trails were categorized and then the types favoring most of participants were verified. There are total number of 16 visual landscape types preferred by participants at both the Dulle-gil and the uphill trail in Bukhansan National Park. As in terms of consensus photography(CP) on the Dulle-gil was found to have more diverse elements including both natural and artificial elements compare to the uphill trail. Furthermore, the perceptually excited node(PEN) at the Dulle-gill were found to be more scattered evenly whereas PEN at the uphill trail were concentrated on the entrance and the top of the mountain. It is necessary to consider that the scenic landscape management at the Dulle-gil and the uphill trail require different approaches. Facilities were set based on careful consideration of distinctive geographical features and vegetation in the Dulle-gil. And the management need to be focus on the 'forested area' maintaining natural state in the uphill trail. Futhermore, the result of the CP and PEN in the trails would be helpful to proceed the next step of landscape perception study depending on characteristics of groups.

• Korean Journal of Environment and Ecology
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• v.15 no.3
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• pp.257-266
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• 2001

Trail might be the primary recreational space to appear the use impact by visitors on ecological space in National park. On the basis of this concept, the carrying capacity was estimated on the trail. Impact rating class and pattern of passersby were surveyed on the 22 valid trails in Bukhansan National Park. Using two variables, the width of trail and amount of passersby, the correlation coefficient was analysed and the regression model was derived by raising x to a higher power. And carrying capacity estimation equation was devised by considering the pay fee visitors and average passersby in a trail section. With carrying capacity estimation equation to apply Bukansan National Park, it is desired that maximum carrying capacity is about 3 million persons a year under conditions of trail width 2.2m when National Park Authority wants to keep the existing management level. If they strengthen the management goal far resource conservation like that they want to keep the trail width 2.0m, the number of visitors might be decreased to about 2 million persons a year.

• Journal of Life Science
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• v.30 no.8
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• pp.661-671
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• 2020

The resistance of cancer cells to anti-cancer drugs is the leading cause of chemotherapy failure. The clinical use of nonsteroidal anti-inflammatory drugs (NSAIDs) has been gradually extended to cancer treatment through combination with anti-cancer drugs. In the current study, we investigated whether NSAIDs including celecoxib (CCB), 2,5-dimethyl celecoxib (DMC), and ibuprofen (IBU) could enhance the cytotoxic effects of imatinib and TNF-related apoptosis inducing ligand (TRAIL) on human cancer cells. We found that the NSAIDs potentiated TRAIL and imatinib cytotoxicity against human hepatocellular carcinoma (HCC) cell lines SNU-354, SNU-423, SNU-449, and SNU-475/TR and against leukemic K562 cells with high level of CD44 (CD44^highK562), respectively. More specifically, CCB induced endoplasmic reticulum stress via up-regulation of ATF4/CHOP which is associated with the induction of autophagy against HCC and CD44^high K562 cells. NSAID-induced autophagic activity accelerated TRAIL cytotoxicity of HCC cells through up- and down-regulation of DR5 and c-FLIP, respectively. The NSAIDs also potentiated imatinib-induced cytotoxicity and apoptosis through down-regulation of markers in CD44^highK562 cells that express a stemness phenotype. Our results suggest that the ability of NSAIDs to induce autophagy could enhance the cytotoxicity of TRAIL and imatinib, leading to a reverse resistance to these drugs in the cancer cells. In conclusion, NSAIDs in combination with low-dose TRAIL or imatinib may constitute a novel clinical strategy that maximizes therapeutic efficacy of each drug and effectively reduces the toxic side effects.

• Korean Journal of Environment and Ecology
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• v.4 no.1
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• pp.63-68
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• 1990

User's impacts on trail deteriorations and edge vegetation were studied in Sokri Mountain National Park in 1990. The entire width, bare width and maximum depth of trail as the trail condition were significantly greater on the more heavily used trail. Deteriorations of trail which were surveyed at the total of 52 were significantly different from those of non-deteriorated points. The dominant trees in the crown layer of trail edge are as follows. According as the altitude rises, the changes happen from Quercus serrata to Quercus variabilis, Pinus densiflora, and to Quercus mongolica at Joongsajaam course, and from Pinus densiflora, Quercus variabilis to Quercus mongolica at Birosanjang course. And Stephanandra incisa, Symplocos chinesis for. pilosa show high relative dominant value at Joongsajaam course, Lindera obtusiloba, Fraxinus sieboldiana show one at Birosanjang course.

• Journal of Life Science
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• v.21 no.11
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• pp.1549-1557
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• 2011

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in many types of transformed cells; however, some human hepatocellular carcinoma cells are particularly resistant to the effects of TRAIL. Although genistein, a natural isoflavonoid phytoestrogen, has been shown to have pro-apoptotic activity against human cancer cell lines, little is known about the mechanism of genistein in terms of TRAIL-induced apoptosis. In the present study, it was investigated whether or not combined treatment with genistein and TRAIL synergistically induced apoptosis in Hep3B hepatocarcinoma cells. Results indicate that treatment with TRAIL in combination with nontoxic concentrations of genistein sensitized TRAIL-resistant Hep3B cells to TRAIL-induced apoptosis, which was associated with mitochondrial dysfunction. Further, the inhibition of p38 mitogen-activated protein kinase (MAPK) activation markedly decreased genistein and TRAIL-induced cell viability and apoptosis by enhanced truncation of Bid, increase of pro-apoptotic Bax, decrease of anti-apoptotic Bcl-2, and release of cytochrome c from mitochondria to cytoplasm. Activation of caspases and degradation of poly (ADP-ribose) polymerase induced by the combined treatment was also markedly increased by the inhibition of p38 MAPK, through the mitochondrial amplification step. In conclusion, our data suggest that genistein sensitizes TRAIL-induced-apoptosis via p38 MAPK-dependent pathway.

• Korean Journal of Environment and Ecology
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• v.19 no.2
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• pp.130-138
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• 2005

The purpose of this study is to analyze the trail deterioration conditions and to obtain the informations for the desirable maintenance and restoration of the trails in Woraksan National Park. To accomplish the purpose, not only trail condition such as altitude, entire width, bare width, maximum depth and slope of trail, but also deterioration types of trail were surveyed at the total 111 points for 13.5km in length on the four access trails to Yeongbong which is the main peak of Woraksan. Furthermore, impact rating class, which could be effective means to check the step and extent of deterioration process, was judged on each points of trail. Results informs us that the deterioration condition of the trail in Woraksan National Park is not reached the lev- el of grave concern yet, but the active counterplan to change over from the safety-centric facility station to the integrated and organized restoration aiming to natural environment conservation is urgently needed.