• Korean Journal of Medicinal Crop Science
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• v.16 no.2
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• pp.86-93
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• 2008

The chemical composition of the volatile constituents from the leaves and stems of endemic Acanthopanax koreanum and A. chiisanensis collected from Mt. Deokyu was determined by GC and GC/MS spectrometric analysis with the aid of NBS, Wiley Library and RI indice searches. The major constituents identified were ${\delta}-3-carene$ (31.34%), l-limonene (17.01 %), ${\beta}-elemene$(4.53%), trans-p-menth-2-ene-1,8-diol(3.13%), 1,8-cineole (4.73%), 1-dodecen-3-yne (2.64%) and (Z)-3,7-dimethyl-1,3,6-octatriene (3.21%) in A. koreanum, and ${\delta}-3-carene$ (14.78%), limonene (7.24%), t-ocimene (7.22%), ${\alpha}-terpinolene$ (8.76%), ${\gamma}-elemene$ (4.32%), ${\beta}-selinene$ (7.72%), veridifloral (3.25%) and dodecane (2.44%) in A. chiisanensis.

• The Journal of Korean Medical History
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• v.17 no.1
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• pp.31-55
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• 2004

For the sake of the Restoration(to the original state) of "Sanbeon-bang(刪繁方)", in this study observed carefully the bibliographical results of "Oedaebiyo-bang", "Cheongeumyo-bang", "Cheongeumik-bang" and "Uisim-bang", which directly quoted "Sanbeon-bang". "Sanbeon-bang" is mostly remained in "Oedaebiyo-bang", and the other provisions of "Sanbeon-bang" mostly lie scattered in "Cheongeumyo-bang" and "Cheongeumik-bang", established 100years before "Oedaebiyo-bang" and "Uisim-bang", established 200years after "Oedaebiyo-bang". And there are few other documents which quoted the "Sanbeon-bang". "Cheongeumyo-bang", a synthetic medical work was compiled in 652, and which refered large quantity of medical documents before Dang-dynasty(唐代) by Son Sa-mak(孫思邈). And he linked the technical opinion of himself. The comments of medicine, as well as the prescriptions are refleting the outcome before Dang-dynasty systematically. For the most part of the medical arguments in "Sanbeon-bang" lie scattered in "Cheongeumyo-bang". "Cheongeumik-bang", also a synthetic medical work which is for the sake of supplement of "Cheongeumyo-bang" was complied in 682 by Son Sa-mak. As well as "CheongeumYo-bang" it doesn't make clear the sources of quotation from "Sanbeon-bang". So it used as the data for confirmation. "Oedaebiyo-bang" was compiled in 752 of Dang-Dynasty by Wang Do(王燾) the a governor-general of Eop-gun. Wang Do was descended from a noble family, and usually visited the palace and dealed with large number of books in Hongmunguan(弘文館), the national library. He programmed the book on the base of wide scopic collection of medical documents. And he took charge of gverment post at the same time as Wang Bing(王氷) who compiled "Chaju-Hwangje-Naegyeong-Somun(次注黃帝內經素問)" in 762. So we can guess they might have seen the same documents and holded the medical knowledge in common. The 40 volume "Oedaebiyo-bang" was a great medical complete book in those days. In particular, it became the model of medical complete books of after ages. The description of the book is mostly the qutation of medical documents of before ages. The character is recording of quotation documents and the order of volume. On the base of the recording, we can reconstruct the table of contents of Sanbeon-bang. By way of the contens, we can understand the purpose of "Sanbeon-bang". Besides, I can see a lot of qouotations of "Sanbeon-bang" in "Cheongeum-bang" and "Uisim-bang" are repeatedly quotated in "Oedaebiyo-bang". For that reason, I observed the bibliographical results of "Oedaebiyo-bang" in detail. "Uisim-bang" was compiled in 982 by the Japanese Niwayasyori(丹波綱賴). It followed the example of the structure of "Oedaebiyo-bang", refered to the contents of "Cheongeumyo-bang", collected chinese medical classic books spreaded in Japan. It contains medical documents of before Su-Dang(隋唐)-era, indicated the source of quotation. The importance of the book is summarization and preservation of large quantity of chinese medicine before 10th century. In this study, a lot of the quotation in "Uisim-bang" from "Sanbeon-bang" were not in "Oedaebiyo-bang".

• Applied Biological Chemistry
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• v.38 no.5
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• pp.382-386
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• 1995

The potato proteinase inhibitor II (PI-II) protein contains chymotrypsin and trypsin inhibitory site. Among several PI-II genes isolated from genomic library, amino acid sequence deduced from PI-IIT gene has 84% identity with that of the polypeptide chymotrypsin inhibitor (PCI). Therefore a gene fragment having homology with the PCI was cloned into a vector using polymerase chain reaction(PCR) from the potato proteinase inhibitor IIT gene. Two different primers were utilized for cloning; primer A contains NdeI restriction site and 30 nucleotides, which has AUG N-terminal methionine codon, primer B contains BclI restriction site and 28 nucleotides, which has TAG translation stop codon. After PCR, about 160 bp-long DNA fragment was cloned into pRT146, derivative of pUC118, and sequenced. The sequenced NdeI/BclI fragment was moved to pET3a, containing bacteriophage T7 promoter and terminator. The expressed proteins in E. coli BL2l(DE3) were determined on a polyacrylamide gel containing sodium dodecyl sulfate. The expected size of protein deduced from the sequenced gene fragment is about 6,500 dalton whose size was similar to the IPTG-induced protein (6,000 dalton) on a gel. However the expression level was much lower than expected.

• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.85-91
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• 2006

Flavanone 3$\beta$-hydroxylase (FHT) is an enzyme acting in the central part of the flavonoid biosynthesis pathway. FHT catalyses the hydroxylation of flavanone to dihydroflavonols in the anthocyanin pathway. In this paper we describe the cloning and expression of the genes encoding the flavonoid-biosynthetic enzyme FHT in Gypsophila paniculata L. A heterologous cDHA probe from Dianthus cavophyllus was used to isolate FHT-encoding cDHA clones from Gypsophila paniculata L.. Inspection of the 1471 bp long sequence revealed an open reading frame 1047 bp, including a 190 bp 5' leader region and 288 bp 3' untranslated region. Comparison of the coding region of this FHT cDHA sequence including the sequences of Arabidopsis thaliana, Citrus sinensis, Dianthus caryophyllus, Ipomoea batatas, Matthiola incana, Nierembergia sp, Petunia hybrida, Solanum tuberosum, Vitis vinifera reveals a identity higher than 69% at the nucleotide level. The function of this nucleotide sequences were verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by northern blotting with mRHA from wild type and mutant plants, by in vitro expression yielding and enzymatically active hydroxylase, as indicated by the small dihydrokaempferol peak. Genomic southern blot analysis showed the presence of only one gene for FHT in Gypsophila paniculata.

• The Journal of Korean Academy of Sensory Integration
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• v.15 no.2
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• pp.93-106
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• 2017

Objective : The purpose of this study is to provide an evidence for clinical practice by systemic analysis on occupational therapy interventions for improvement of children's play. Methods : Articles used in this study were collected from database of Pubmed, ProQuest, EBSCOhost, Scopus, RISS, KISS, and National Library of Korea. The key words used in the search were "Play AND Occupational Therapy AND (Intervention OR Treatment)" and the publication period was limited from January 2007 to April 2017. 11 articles in total were selected for the systematic review analysis. Results : The results revealed that the majority of studies was one-group non-randomized study and the majority of intervention used was play-based intervention in case of that play itself was the intervention goal. The most frequently used environment for the intervention was parallel setting of home and treatment room. The majority of studies was for children with ADHD and the most commonly used assessment tool was Test of Playfulness (ToP). Dependent variables measured along with play were language skills, social interactive skills, communication and pragmatic skills, problem-solving skills, caregiver responsiveness, and parent-child interaction. Conclusion : This study help to understand the current state of occupational therapy intervention for improvement of children's play. Starting with the understanding, it is expected that various studies on play of children will be done in the future.

• Journal of the Korean Geographical Society
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• v.46 no.6
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• pp.707-723
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• 2011

The purpose of this study was to quantitatively evaluate the effectiveness of learning management systems (LMS) in the physical geography class. The study adopted the experimental design and three classes participated in this study. The first class was controlled using only classroom lectures, the second class used PPT slides along with the classroom lectures, and the third class used online video clips along with the lectures. The experiments were conducted from the Spring Semester 2007 to the Spring Semester 2008 for the introductory physical geography course. The study results showed that online learning tools help students improve academic performance and their attitudes towards the class and the instructor. Compared to simple PowerPoint slides, voice recording attached to the visual lecture slide materials enhanced students' motivation. Class lectures with lecture slides did not improve students' scores. However, when the visual materials were combined with voice recording, the number of internet access to online class materials increased, and class attendance and students' final grades were improved. Based on the results, the instructional design model that combines classroom and online learning was suggested.

• Journal of Industrial Convergence
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• v.18 no.5
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• pp.42-53
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• 2020

This study approached the elderly in Korea with a systematic review to find out the effect of virtual reality program arbitration on balance, which the evidence for the virtual reality program is provided. Total of 94 papers were searched through the database Nuri Media (DBpia), Scholarship (earticle), Korean Studies Information (KISS), National Digital Science Library (NDSL), the Korea Educational Research and Information Service (RISS), Kyobo Book Scholar (RISS), and Hakjisa New Thesis on Literature Selection using PRISMA flow-chart from January 2005 to May 2020 based on the final literature selection process and analysis. The quality level of the literature was found to be three volumes (50.0%) of the base level I, one (16.7%) of the II, and two of the III (33.3%). The most common type of virtual reality program was Wii-fit balance of 4 (66.7%), and the effect of virtual reality program arbitration was significant overall through evaluation tools for balance and walking ability. This is expected to effectively apply the virtual reality program to the elderly. In addition, since clinical application basis has been provided, further studies applying various virtual reality program interventions need to be addressed.

• Journal of Korean Medicine for Obesity Research
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• v.20 no.1
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• pp.52-68
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• 2020

Objectives: The purpose of this study is to investigate the weight loss effects of auricular acupuncture in obese patients. Methods: Six databases (Research Information Sharing Service [RISS], Korean studies Information Service System [KISS], Oriental Medicine Advanced Searching Integrated System [OASIS], PubMed, The Cochrane Library, China National Knowledge Infrastructure [CNKI]) were searched up to May 20, 2020. Eight eligible randomized controlled trials were included the present study. The quality of included studies was assessed by the Cochrane risk of bias tool and a meta-analysis was performed by Review Manager software. A meta-analysis was conducted using a random-effects model and a subgroup analysis was performed to detect the sources of heterogeneity, identify the selection of acupuncture methods and explore its contributions to the weight loss effects. Results: Among 8 trials, 5 trials used auricular acupuncture and 2 trials used auricular acupressure, 1 trial used both types of intervention. Most commonly selected acupoints were Shenmen (TF4) and stomach (CO4). Treatment duration was six to twelve weeks, and total treatment session was six to twelve. Compared to the control groups, auricular acupuncture significantly decreased body weight, body mass index (BMI), high density lipoprotein-cholesterol (HDL) and ghrelin. For the selection of acupuncture methods, both methods performed similarly in most outcome except waist circumference (WC), body fat percentage (BFP), and triglycerides (TG). Conclusions: We found that auricular acupuncture can be effective for weight loss and controlling appetite. However, the findings should be interpreted with caution due to heterogeneity. So further vigorous and well-designed studies should be conducted to strengthen the evidence of the use of auricular acupuncture for obesity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7394-7402
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• 2015

The purpose of this study was to investigate the status of studies on swallowing rehabilitation interventions for stroke patients that had been conducted in the past 5 years (2010-2014) in Korea and to examine their findings. A literature search was conducted through RISS, National Assembly Library, and KISS, and 12 papers were analyzed. Four articles were from nursing science, and 8 from other disciplines. Qualitative evaluation of the articles was conducted by using an evaluation framework that was designed by the researcher, which took into account the checklist presented by the Scottish Intercollegiate Guideline Network (2013). In the results of the qualitative evaluation on the papers, 4 studies scored 7 out of 10 points, 6 studies scored between 5 and 6 points, and 2 studies scored 4 points. Intervention sessions were conducted for 25 minutes, on average, each at a frequency of 4.1 sessions per week, for a total of 33.7 sessions. This study found that direct swallowing training and comprehensive intervention of respiratory and oral, including pharyngeal were effective in relieving difficulties in swallowing. In the future, such findings are expected to be used in nursing interventions for establishment of the basis for evidence-based nursing practice.

• Journal of Information Management
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• v.43 no.2
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• pp.67-83
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• 2012

Patents are the sources reflecting technology development by research and development(R&D) as well as the tools to secure economic benefits in the market, so using patent information is crucial for decision making processes in formulating technology development strategies. Intellectual property(IP) portfolios including a set of patents related to products and individual technologies are the basic unit that has the economic meaning in making national policies and technology strategies. Therefore, this research develops a total of 69 measures to identify the collective characteristics for IP portfolios("characteristics index"), by incorporating the patent indexes that have been widely used and the patent indexes that developed recently, and applying the concepts to patent analysis that have been used in interdisciplinary studies including economics and library and information science. The results of this research produced a characteristics index manual which helps experts to identify characteristics of technological innovation systems from various dimensions. We expect that the characteristics indexes can be used as a supportive tool for comparative analysis among IP portfolios in the technology policy making process.