• 제목/요약/키워드: National development

검색결과 50,195건 처리시간 0.068초

Development of EST-SSRs and Assessment of Genetic Diversity in Germplasm of the Finger Millet, Eleusine coracana (L.) Gaertn.

  • Wang, Xiaohan;Lee, Myung Chul;Choi, Yu-Mi;Kim, Seong-Hoon;Han, Seahee;Desta, Kebede Taye;Yoon, Hye-myeong;Lee, Yoonjung;Oh, Miae;Yi, Jung Yoon;Shin, Myoung-Jae;Kim, Kyung-Min
    • 한국작물학회지
    • /
    • 제66권4호
    • /
    • pp.443-451
    • /
    • 2021
  • Finger millet (Eleusine coracana) is widely cultivated in tropical regions worldwide owing to its high nutritional value. Finger millet is more tolerant against biotic and abiotic stresses such as pests, drought, and salt than other millet crops; therefore, it was proposed as a candidate crop to adapt to climate change in Korea. In 2019, we used expressed sequence tag simple sequence repeat (EST-SSR) markers to evaluate the genetic diversity and structure of 102 finger millet accessions from two geographical regions (Africa and South Asia) to identify appropriate accessions and enhance crop diversity in Korea. In total, 40 primers produced 116 alleles, ranging in size from 135 to 457 bp, with a mean polymorphism information content (PIC) of 0.18225. Polymorphism was detected among the 40 primers, and 13 primers were found to have PIC values > 0.3. Principal coordinate and phylogenetic analyses, based on the combined data of both markers, grouped the finger millet accessions according to their respective collection areas.Therefore, the 102 accessions were classified into two groups, one from Asia and the other from Africa. We have conducted an in-depth study on the finger millet landrace pedigree. By sorting out and using the molecular characteristics of each pedigree, it will be useful for the management and accession identification of the plant resource. The novel SSR markers developed in this study will aid in future genetic analyses of E. coracana.

Snapshot View of Expressed Sequences from Brassica rapa

  • Lim, Myung-Ho;Kim, Jin-A;Lee, Ji-Young;Park, Yang-Sun;Lee, Soo-In;Kim, Jung-Sun;Kim, Seog-Hyung;Kwon, Soo-Jin;Lim, Ki-Byung;Yang, Tae-Jin;Jin, Min-A;Park, Kie-Jung;Chung, Yong-Yoon;Lim, Chae-Oh;Hur, Yoon-Kang;Kim, Ho-Il;Park, Beom-Seok
    • 한국식물생명공학회:학술대회논문집
    • /
    • 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
    • /
    • pp.199-199
    • /
    • 2005
  • PDF

Assessment of the resistance of bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum KACC 21701 in Kimchi cabbage genetic resources

  • Parthiban Subramanian;Ho Chul Ko;Seong-Hoon Kim;Jae Eun Lee;Aejin Hwang;Bichsaem Kim;Yoon-Jung Lee;Awraris Derbie Assefa;Onsook Hur;Nayoung Ro;Jung Sook Sung;Ju Hee Rhee;Ho-Sun Lee;Bum-Soo Hahn
    • 환경생물
    • /
    • 제40권4호
    • /
    • pp.433-441
    • /
    • 2022
  • Bacterial phytopathogen Pectobacterium causes soft rot disease in several vegetable crops globally, resulting in heavy agricultural losses at both the pre and postharvest stages. The present work was carried out to screen Kimchi cabbage genetic resources conserved at the National Agrobiodiversity Center, Rural Development Administration, Korea, for resistance against the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum KACC 21701 over a period of three years (from 2020 to 2022). Infection of the phytopathogen was carried out at four-leaf stage and for each accession, twenty-five plants per germplasm were infected with KACC 21701. Kimchi cabbage cultivars Wangmatbaechu, Seoulbaechu, and CR Kiyoshi were used as control. Seven-days post-infection, the Disease Index (DI) values were manually recorded from zero to four, zero matched perfectly heathy plants and four completely dead plants. The 682 accessions of Kimchi cabbage exhibited varying degrees of disease resistance to KACC 21701 and thirty accessions, exhibiting a DI≤2, were considered for replication studies. During the replication studies, four landrace germplasms (IT102883, IT120036, IT120044, and IT120048) and one cultivar(IT187919) were confirmed to be moderately susceptible to KACC 21701. Results of the preliminary screening as well as replication studies were documented for the all the 682 germplasms. Addition of such information to the passport data of stored germplasms might serve as potential bio-resource for future breeders and researchers to develop resistant varieties or study the mechanisms involved in resistance of plants to such phytopathogen.

Risk of Recrudescence of Lymphatic Filariasis after Post-MDA Surveillance in Brugia malayi Endemic Belitung District, Indonesia

  • Santoso, Santoso;Yahya, Yahya;Supranelfy, Yanelza;Suryaningtyas, Nungki Hapsari;Taviv, Yulian;Yenni, Aprioza;Arisanti, Maya;Mayasari, Rika;Mahdalena, Vivin;Nurmaliani, Rizki;Marini, Marini;Krishnamoorthy, K.;Pangaribuan, Helena Ullyartha
    • Parasites, Hosts and Diseases
    • /
    • 제58권6호
    • /
    • pp.627-634
    • /
    • 2020
  • Belitung district in Bangka-Belitung Province, Indonesia with a population of 0.27 million is endemic for Brugia malayi and 5 rounds of mass drug administration (MDA) were completed by 2010. Based on the results of 3 transmission assessment surveys (TAS), the district is declared as achieving elimination of lymphatic filariasis (LF) in 2017. The findings of an independent survey conducted by the National Institute of Health Research and Development (NIHRD) in the same year showed microfilaria (Mf) prevalence of 1.3% in this district. In 2019, NIHRD conducted microfilaria survey in 2 villages in Belitung district. Screening of 311 and 360 individuals in Lasar and Suak Gual villages showed Mf prevalence of 5.1% and 2.2% with mean Mf density of 120 and 354 mf/ml in the respective villages. Mf prevalence was significantly higher among farmers and fishermen compared to others and the gender specific difference was not significant. The results of a questionnaire based interview showed that 62.4% of the respondents reported to have participated in MDA in Lasar while it was 57.7% in Suak Gual village. About 42% of the Mf positive cases did not participate in MDA. Environmental surveys identified many swampy areas supporting the breeding of Mansonia vector species. Persistence of infection is evident and in the event of successful TAS3 it is necessary to monitor the situation and plan for focal MDA. Appropriate surveillance strategies including xenomonitoring in post-MDA situations need to be developed to prevent resurgence of infection. Possible role of animal reservoirs is discussed.