• Journal of Korean Society of Forest Science
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• v.98 no.2
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• pp.142-147
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• 2009

Hyoscyamus species is sources of the hypnotic and sedative drugs hyoscyamine and scopolamine. Single cells of Hyoscyamus niger were dissociated from suspension cultures and adventitious roots obtained from single-cell clones which were cultured on B5 medium containing 3% (w/v) sucrose, 0.1 mg/L IBA and 0.4% (w/v) gelrite. H. niger adventitious root lines showed wide variation in tropane alkaloids production and growth. An effective selection of 200 root lines was made possible by the application of the 'Dragendorff's reagent' for qualitative detection of the alkaloids from root. A high correlation coefficient (r=0.9390) was observed between the values obtained with the two methods based on HPLC and Dragendorff's reagent analysis. Among the selected roots, the highest scopolamine content was 16.64 mg/g DW (Hn-59), which was 8.82-fold more productive than the lowest alkaloid producing line (Hn-25). Here, we established an efficient selection method on tropane alkaloids production and suggest that the Dragendorff's reagent is of great practical value in selection of invisible compounds.

• Journal of Forest and Environmental Science
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• v.39 no.4
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• pp.246-253
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• 2023

Salix gracilistyla is widely distributed along riversides in Korea and very good for biomass production by SRC because of its excellent germination ability, but it is necessary to measure drying tolerance for cultivation. The drought tolerance of S. gracilistyla was tested using cuttings, and growth and physiological analysis were performed after irrigation was stopped. The growth inhibition of S. gracilistyla was observed from the day irrigation was stopped, and the soil moisture content decreased to less than 10% on the 25th day after irrigation was stopped. Over 50% of the seedlings turned brown 25 days after watering was stopped. The chlorophyll content of S. gracilistyla decreased dramatically after 25 days of stopping of irrigation. RWC values were unchanged until day 12 after irrigation was stopped but decreased rapidly until day 21, but there was a slightly decreasing trend after that. RWL levels increased slightly during irrigation stops. The proline content of plants subjected to drought stress was 0.91-2.63 mg/0.05 g, 2.75 times higher than that of the control treatment. The sugar content of the drought stress treatment group was 29.77 to 350.66 mg/0.05 g, which increased 12.24 times that of the control treatment. As a result of this study, S. gracilistyla was found to have a drought tolerance almost comparable to that of evergreen broad-leaved trees growing on the land. This study is expected to contribute to the resource utilization S. gracilistyla, a native willow tree of Korea, and the mass production of biomass by SRC.

• Journal of the Korean Wood Science and Technology
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• v.45 no.4
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• pp.419-431
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• 2017

The forest slopes cause substantial local changes in soil properties and an increase in soil erosion after extreme rainstorms. The high soil erosion rates on forest slopes need the effective use of growing media to control the soil runoff. Therefore, we prepared six different lignocellulosic growing media such as peat, perlite, and wood meal as the base materials and carboxymethyl cellulose (CMC), glucomannan, starch, old corrugated containerboard, and computer printout as the additional materials for the prevention of simulated rainfall-induced runoff. The growing media containing old corrugated containerboard efficiently reduced the percentage of soil runoff; however, it could not completely cushion the influence of crust. The best results for plant growth, except in the leaf area, were also obtained with the growing media containing old corrugated containerboard, suggesting an interesting way of paper recycling and an economic benefit for plant or crop growth in forest slope.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.285-294
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• 2021

In general, mycoviruses remain latent and rarely cause visible symptoms in fungal hosts; however, some viral infections have demonstrated abnormal mycelial growth and fruiting body development in commercial macrofungi, including Lentinula edodes. Compared to other cultivated mushrooms, L. edodes is more vulnerable to viral infections as it is still widely cultivated under near-natural conditions. In this study, we investigated whether Korean wild strains of L. edodes were infected by RNA mycoviruses that have previously been reported in other parts of the world (LeSV, LePV1, LeV-HKB, LeNSRV1, and LeNSRV2). Using specific primer sets that target the RNA-dependent RNA polymerase genes of each of the RNA mycovirus, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect viral infection. Viral infection was detected in about 90% of the 112 wild strains that were collected in Korea between 1983 and 2020. Moreover, multiple infections with RNA mycoviruses were detected in strains that had normal fruiting bodies. This work contributes to our understanding of the distribution of RNA mycoviruses in Korea and the impact of multiple viral infections in a single strain of L. edodes.

• Korean Journal of Plant Resources
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• v.35 no.6
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• pp.778-784
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• 2022

Wild vegetables, such as Cirsium setidens, and Aster scaber, are commonly distributed as dried materials because the wild vegetables lose their freshness quickly after harvest and distribution. Dried wild vegetables require rehydration to use as a food ingredient, and the quality of rehydrated wild vegetables is affected by pre-drying and drying methods. Here, we introduce the newly developed pre-drying and drying method, termed "micro oil sprayed thermal air (MOTA) technique". The three wild vegetables processed by the MOTA technique showed improved rehydration rate and reduced time to achieve maximum rehydration rate. Color characteristics were also improved in C. setidens. These results indicate that the MOTA technique improves the overall quality of rehydrated wild vegetables. It is expected that our findings could enhance the marketability of dried wild vegetables by improving overall quality and reducing preparation time.

• Journal of Forest and Environmental Science
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• v.36 no.1
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• pp.62-66
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• 2020

Ethanol fermentation of the enzymatic hydrolysates from the products pretreated using 1-ethyl-3-methyl-imidazolium acetate ([EMIM]Ac) and its co-solvents with dimethylformamide (DMF) was conducted using Saccharomyces cerevisiae (D452-2). The optical density change due to the yeast cell growth, the consumption amount of monosugars (glucose, xylose), the concentration of acetate, and ethanol production yield were investigated. The co-solvent system lowered inhibition of the growth of the cells. The highest concentration of glucose (7.8 g/L) and xylose (3.6 g/L) was obtained from the enzymatic hydrolysates of the pretreated product by pure [EMIM]Ac. The initial concentration of both monosugars in the enzymatic hydrolysates was decreased with increasing fermentation time. Ethanol of Approximately 3 g/L was produced from the enzymatic hydrolysates by pure [EMIM]Ac and co-solvent with less than 50% DMF.

• The Korean Journal of Mycology
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• v.49 no.4
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• pp.525-530
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• 2021

The cultivation in sawdust media, mushroom productivity, and fruiting body characteristics of Lentinula edodes strains NIFoS 2778 and NIFoS 3363 were compared according to the inoculation conditions. The cultivation period was 5% shorter when liquid spawn was used. Fruiting bodies were induced after 113 days of incubation on media inoculated with liquid spawn, and the cultivation period was 119 days on media inoculated with solid spawn. Mushroom productivity of NIFoS 2778 was the highest (661.4 g) when 36 mL of liquid spawn was used. For NIFoS 3363, mushroom production was higher under liquid inoculation conditions when the same amount of liquid and solid spawns were used. The mushroom characteristics of the two strains were not significantly different, except for gill width and stipe diameter.

• Journal of Forest and Environmental Science
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• v.36 no.2
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• pp.69-77
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• 2020

Lignocellulosic biomass has recalcitrant characteristics against chemical and biological conversion due to its structural heterogeneity and complexity. The pretreatment process to overcome these recalcitrant properties is essential, especially for the biochemical conversion of lignocellulosic biomass. In recent years, pretreatment methods using ionic liquids (ILs) and deep eutectic solvents (DESs) as the green solvent has attracted great attention because of their advantages such as easy recovery, chemical stability, temperature stability, nonflammability, low vapor pressure, and wide liquids range. However, there are some limitations such as high viscosity, poor economical feasibility, etc. to be solved for practical use. This paper reviewed the research activities on the pretreatment effect of various ILs including DESs and their co-solvents with organic solvents on the enzymatic saccharification efficiency of lignocellulosic biomass and the nanocellulose preparation from the pretreated products.

• Korean journal of applied entomology
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• v.59 no.4
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• pp.409-410
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• 2020

This report describes the damage caused by gypsy moths (Lymantria dispar) and stick bugs (Ramulus mikado) to forests and host plants in 2020. Severe tree-crown defoliation was found in central parts of Korea, especially Gangwon (1,638 ha), Gyeonggi (1,134 ha), Chungbuk (726 ha) and Seoul (476 ha). Stick bug outbreaks occurred in hilly areas, such as Mt. Bongsan, located between Seoul and Goyang.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.383-395
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• 2016

This study investigated the effect of exogenous hydrogen peroxide ($H_2O_2$) on the antioxidant responses and growth of warm-season turfgrass (Zoysia japonica Steud.) and cool-season turfgrass (Poa pratensis L.) subjected to drought stress. Compared with control plants that were not pretreated with $H_2O_2$, plants pretreated with $H_2O_2$ had significantly greater fresh and dry weights of shoots and roots, and increased water content. $H_2O_2$ pretreatments before drought stress significantly decreased the concentrations of malondialdehyde and $H_2O_2$. DPPH radical scavenging and glutathione activities were significantly increased. The responsive activities of the antioxidant enzymes superoxide dismutase, ascorbate peroxidase, catalase, and peroxidase were also significantly enhanced. Our results suggest that exogenous $H_2O_2$ could improve the growth of warm-season and cool-season turfgrass under drought stress by increasing the activity of their antioxidant enzymes, while decreasing lipid peroxidation.