• 미생물학회지
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• 제17권1호
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• pp.25-41
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• 1979

A strain able to grow up m-toluate minimal medium has been isolated after selective enrichment and given the name T81X, which was later identified as pseudomonase putida according to its morphological and biochemical characteristics. After treatment with plasmied specific curing agent, mitomycin C, followed by replica plating on m-toluate and xylene minimal agar plate, T81Xstrain has been shown to harbour a curable plasmid relating to the m-toluate and xylene metabolism. Spontaneous curing frquency of this plasmid was also greatly enhanced by growing on benzoate minimal medium. After then, it was also xylene metabogrowing on benzoate minimal medium. After then, it was found to be conjugally nontransmissible. From the comparative investigation of catechol 1,2-oxygenase and catechol 2,3-oxygenase activities in wild type and cured strain on various growth substrate, it appeared that T81X strain has both of these two enzymes while cured strain has catechol 1,2-oxygenase only. Growing on m-toluate minimal medium T81X strain should carry the genetic information necessary for coding the catechol 1,2-oxygense induced by m-toluate or benzoate, on that curable plasmid.

• 한국수산과학회지
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• 제54권5호
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• pp.782-786
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• 2021

A single specimen (244.2 mm in standard length) of Satyrichthys welchi was collected from Pohang in the southern East Sea of Korea in March 2021. This species is characterized by the following morphological combination: 4 lip barbels, 3 chin barbels, antrose spines on upper lateral low of posterior bony plate and equal parietal bones. S. welchi was morphologically similar to its congeneric species, S. laticeps, but is clearly distinguished in presence of dusky black spots on dorsal fin (present in S. welchi vs. absent in S. laticeps). We propose a new Korean name "Gi-JeomByeol-Seong-Dae" for the species S. welchi.

• 식물분류학회지
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• 제32권3호
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• pp.363-371
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• 2002

선만실용임업편람(鮮滿實用林業便覽) 및 Mori의 한선식물명휘(韓鮮植物名彙)에 실린 Nakai의 많은 나명들이 비합법적으로 발표된 이름임에도 불구하고 여러 식물도감에서 지속적으로 잘못 사용하고 있다. 지난 30여년간 일부 국내학자들에 의한 새로운 조합명도 기본명이 발표된 문헌과 저자, 발표년도 등에 대한 기재가 없어 비합법적으로 발표된 학명으로 확인되었다. 또한, 신종기재시 요구되는 기재와 표징뿐 아니라 기준표본에 대한 언급을 명확하게 밝히지 않거나 혹은 문헌상으로만 기준표본이 존재하고 실질적으로 기준 표본을 보관하지 않은 식물명은 모두 나명이거나 혹은 비합법적으로 발표된 학명들이다. 이러한 학명들은 더 이상 사용되어서는 안되는 이름들이다. 목본식물 중 이전의 문헌에서 간과하기 쉬운 비합명을 정리한 본 연구가 다른 연구자들에게 도움이 되길 바라며 초본에서도 이와 같은 연구가 시도될 수 있기를 바란다.

• 대한치과교정학회지
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• 제25권6호
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• pp.715-722
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• 1995

Orthodontic tooth movement in response to orthodontic force results from actions of osteoclasts and osteeoblasts in the cell level. Convincing evidence has now been provided to support the view that osteoclasts are derived from mononuclear cells that originate in the bone marrow or other hematopoietic organs and they migrate to the bones via vascular routes. Nitric oxide(NO), which accounts for the biological properties of endothelium-derived relaxing factor(EDRF), is the endogenous stimulator of soluble guanylate cylase. The discovery of the formation of nitric oxide(NO) from L-arginine in mammalian tissues and its biological roles has, in the last 7 years, thrown new light onto many areas of research. Data from experiments in vitro showed that N-metyl-L-arginine(L-NMA) and L-nitro-L- arginine(L-NAME) are competitive inhibitors of nitric oxide synthase. This study suggest that the multinucleated cells in our culture have characteristics of osteoclasts and that the potential bone cell activity of nitric oxide in vitro may be mediated in part by stimulation of marrow mononuclear cells to form osteoclast-like cells. Bone marrow cells were obtaineed from tibia of 19-days old chick embryo. After sacrifice, tibia was quickly dissected and the bone were then split to expose the medullary bone. The cells were attached for 4 hours and the nonadherent cells were collected. Marrow cells weere cultured in 96-well plate in medium 199. To examine the number of TRAP-positive multinucleated cells(MNCs), $10^{-8}\;M\;Vit=D_3$ and various concentration of L-NMA and L-NAME weere added at the beginning of cultures and with each medium change. After 7 days of culture. tartrate-resistant acid phosphatase(TRAP) staining was performed for microscopic evaluation. Cells haying more than three nuclei per cell were counted as MNCs. The obsrved results were as follows;1. 1,25-dihydroxyvitamine $D_3$ stimulated the osteoclast-like multinucleated cells in cultures of chick embryo bone marrow. 2. Nitric oxide synthase inhibitors(NOSI ; N-NMA, N-NAME) stimulated the osteoclast-like cells in cultures of chick embry bone marrow. 3. 1,25-dihydroxyvitamine$D_3$ and nitric oxide synthase inhibitors did not appear to have additive effect on the generation of TRAP-positive MNCs. These results suggest that nitric oxide synthase inhibitors may stimulate the osteoclast-like multinucleated cell formation and fusion in cultures of chick bone marrow.

• Journal of Plant Biotechnology
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• 제6권1호
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• pp.9-13
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• 2004

Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

• 한국어류학회지
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• 제19권1호
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• pp.70-72
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• 2007

우리나라의 동해 연안에서 출현하는 날개줄고기과 어류 1개체를 동정한 결과 지금까지 우리나라에서는 기록되지 않은 Hypsagonus corniger Taranetz로 확인되었다. 본종은 형태적으로 뿔줄고기와 유사하지만, 제1등지느러미 가시는 7개이고, 후두부 가시가 길고, 체측 상부 골질판에는 가시가 없고, 체측 가시 끝이 예리하며, 제1등지느러미와 제2등지느러미 사이는 제2등지느러미 기저 길이보다 크고, 제1등지느러미의 제1가지와 제2가시의 길이 차이가 큰 점 등이 특징적이다. 본 종의 신한국명은 몸에 예리한 가시가 많아서 "가시줄고기"라고 명명하였다.

• 전력전자학회논문지
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• 제9권1호
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• pp.42-49
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• 2004

본 논문은 데드비트 외란 관측기를 사용한 외부 부하 외란 보상과 파라미터 추정기에 의한 보상 이득의 조정을 나타내고 있다. 결론적으로 PMSM의 응답은 지표 시스템을 따른다. 부하 토크 보상 방법은 데드비트 관측기로 구성된다. 노이즈 영향을 감소시키기 위해 MA 처리에 의해 구현된 후단 필터를 적용하였고, RLSM 파라미터 추정기를 가진 파라미터 보상기가 주어진 실제 시스템의 이득 계산시 사용된 파라미터로 가상 동작하여 이득이 오차가 없는 것처럼 동작하게 한다. 제안된 추정기는 문제를 풀기 위해 고성능 외란 관측기와 조합하여 사용한다. 제안된 제어 시스템은 부하토크와 파라미터 변화에 대해 강인하고 정밀한 시스템이 된다. 이상의 제안된 시스템의 안정성과 유용함이 컴퓨터 시뮬레이션과 실험을 통하여 확인되었다.

• 한국가구학회지
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• 제27권2호
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• pp.104-110
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• 2016

The aim of this study is to develop and suggest the handle for lockers according to the design process which are used for keeping of student's personal stuffs such as books, writing implements and gym uniform in elementary, middle and high school and university as well. This includes the design development for ILGUMTUR which is specialized for office furniture, but does not own the intellectual property right yet. The design concept based on the domestic locker market survey is recessed handle at low- to intermediate price and it's material should be plastic what is solid and light. The lock system should be constructed by molding process and the name plate can be exchanged every time the owner of locker changes. Contrary to the right angle of it's external appearance, the handle of the locker is designed to be softly round and the simplicity is pursued for an easy production. On the basis of this design concept the sketch has been drawn and upgraded in full by 3D-rendering. Then the mock-up has been completed by 3D printer and ILGUMTUR has obtained a patent for the locking system based on the rotation of 2 circles.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2015년도 춘계 학술논문 발표대회
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• pp.183-184
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• 2015

Recent structural approach of the House of Commons has been an increase in demand for dry wall light due to a change in load bearing composite structure of a flat plate type structure under the name of long-life housing, attach the attachment to the wall as the needs of the residents of reserved space increases things that are on the rise. However, depending on the strength of collapse accidents is shown wall and recent wall deposits are required, a load resistance evaluation method of the deposit of the need is the situation. Therefore, in this study, for the evaluation of the evaluation of dry lightweight wall as widely used gypsum board and ALC lightweight wall of two types of laboratory the wall through the previous studies to be installed in direct co-housing to the target and field experiments I want to evaluate the different points.

• Structural Engineering and Mechanics
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• 제31권2호
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• pp.181-210
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• 2009

This paper presents the theoretical developments of an exact finite strip for the buckling and initial post-buckling analyses of isotropic flat plates. The so-called exact finite strip is assumed to be simply supported out-of-plane at the loaded ends. The strip is developed based on the concept that it is effectively a plate. The present method, which is designated by the name Full-analytical Finite Strip Method in this paper, provides an efficient and extremely accurate buckling solution. In the development process, the Von-Karman's equilibrium equation is solved exactly to obtain the buckling loads and the corresponding form of out-of-plane buckling deflection modes. The investigation of thin flat plate buckling behavior is then extended to an initial post-buckling study with the assumption that the deflected form immediately after the buckling is the same as that obtained for the buckling. It is noted that in the present method, only one of the calculated out-of-plane buckling deflection modes, corresponding to the lowest buckling load, i.e., the first mode is used for the initial post-buckling study. Thus, the postbuckling study is effectively a single-term analysis, which is attempted by utilizing the so-called semi-energy method. In this method, the Von-Karman's compatibility equation governing the behavior of isotropic flat plates is used together with a consideration of the total strain energy of the plate. Through the solution of the compatibility equation, the in-plane displacement functions which are themselves related to the Airy stress function are developed in terms of the unknown coefficient in the assumed out-of-plane deflection function. These in-plane and out-of-plane deflected functions are then substituted in the total strain energy expressions and the theorem of minimum total potential energy is applied to solve for the unknown coefficient. The developed method is subsequently applied to analyze the initial postbuckling behavior of some representative thin flat plates for which the results are also obtained through the application of a semi-analytical finite strip method. Through the comparison of the results and the appropriate discussion, the knowledge of the level of capability of the developed method is significantly promoted.