• Parasites, Hosts and Diseases
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• 제55권1호
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• pp.47-54
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• 2017

The prevalence of cercarial infection in freshwater snails and their evolutionary trends were studied in Nakhon Nayok province, Thailand. A total of 2,869 individual snails were examined for parasitic infections. The results showed that 12 snail species were found to host larval stages of trematodes with an overall prevalence of 4.7%. The infected specimens included 7 types at the cercarial stage; cercariae, megalurous cercariae, echinostome cercariae, furcocercous cercariae, parapleurolophocercous cercariae, virgulate cercariae, and xiphidiocercariae. Regarding molecular identification, ITS2 sequence data of each larval trematode were analyzed, and a dendrogram was constructed using the neighbor-joining method with 10,000 replicates. The dendrogram was separated into 6 clades (order/family), including Echinostomatida/Echinostomatidae, Echinostomatida/Philophthalmidae, Opisthorchiida/Heterophyidae, Plagiorchiida/Prosthogonimidae, Plagiorchiida/Lecithodendriidae, and Strigeatida/Cyathocotylidae. These findings were used to confirm morphological characteristics and evolutionary trends of each type of cercariae discovered in Nakhon Nayok province. Furthermore, this investigation confirmed that the ITS2 data of cercariae could be used to study on phylogenetic relationships or to determine classification of this species at order and/or family level when possible.

• Journal of Applied Biological Chemistry
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• 제60권1호
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• pp.41-47
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• 2017

Genetic differentiation and antioxidant activities in ethanolic extracts from leaves of Bouea macrophylla Griffith were determined. The result revealed genetic differentiation among sour ma-praang, ma-yong and sweet ma-praang of B. macrophylla Griffith (${\phi}_{PT}=0.772$, p-value=0.000). In addition, high genetic diversities were found in sour ma-praang and sweet ma-praang populations (P: 51.4 and 57.1 %; He: 0.1900.035 and 0.2240.036, respectively), but low genetic diversity was found in ma-yong population (P: 8.6 %; He: 0.0350.021). Total phenolic contents of sour ma-praang, ma-yong and sweet ma-praang were estimated as $680.51{\pm}89.81$, $701.03{\pm}59.89$ and $530.85{\pm}41.23mg$ gallic acid/g extract, respectively. Free radical scavenging activities of sour mapraang, ma-yong and sweet ma-praang were found (1/EC50=4.17, 1.43 and 1.37, respectively) corresponding with metalchelating activities (1/EC50=0.83, 0.65 and 0.17, respectively). Therefore, the obtained data may be applied to cultivation and utilization of their leaves as source of natural phenolics and antioxidants.