• Journal of Microbiology and Biotechnology
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• v.28 no.2
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• pp.323-329
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• 2018

In Escherichia coli, the transcription of genes related to metal homeostasis is activated by the presence of target metals. The promoter regions of those genes can be fused with reporter genes to generate whole-cell bioreporters (WCBs); these organisms sense the presence of target metals through reporter gene expression. However, the limited number of available promoters for sensing domains restricts the number of WCB targets. In this study, we have demonstrated an alternative method to generate novel WCBs, based on the notion that since the sensing mechanisms of WCBs are related to metal transportation systems, their properties can be modulated by disrupting metal homeostasis. Mutant E. coli strains were generated by deleting the znt-operon genes zntA, which encodes a zinc-export protein, and zntR, which encodes a znt-operon regulatory protein, to investigate the effects on the metal-sensing properties of WCBs. Deletion of zntA increased the sensitivity but abolished the selectivity of cadmium-sensing WCBs, whereas arsenic-sensing WCBs gained sensitivity toward cadmium. When zntR was deleted, cadmium-sensing WCBs lost the ability to detect cadmium, and this was recovered by introducing exogenous zntR. In addition, the metal-binding site of ZntR was genetically engineered to modulate metal selectivity. This study provides a valuable platform for the development of novel E. coli-based WCBs.

• Journal of Korean Society for Atmospheric Environment
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• v.24 no.4
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• pp.393-403
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• 2008

In an effort to estimate secondary $PM_{10}$ concentrations and their diurnal variations at different photochemical activities, $PM_{10}$, CO, and $O_3$ concentrations obtained from the ambient air quality network located in Seoul are analyzed for the period from 2000 to 2005. In order to classify the photochemical activities on a daily basis, measured ${\Delta}O_{3,\;max-min}$ (maximum $O_3$-minimum $O_3$) and ${\int}(hv)dt$ which represents accumulated daily insolation, were used to classify each day into three regimes: 1) low photochemical reactivity; ${\Delta}O_{3,\;max-min}\;{\leq}\;40\;ppb$, and ${\int}(hv)dt\;{\leq}\;4000\;W/m^2$, 2) moderate photochemical reactivity; $40\;ppb\;<\;{\Delta}O_{3,\;max-min}\;{\leq}\;60\;ppb$, and $4000\;{\leq}\;{\int}(hv)de\;{\leq}\;6000\;W/m^2$, and 3) high photochemical reactivity; ${\Delta}O_{3,\;max-min}\;>\;60\;ppb$, and ${\int}(hv)dt\;{\geq}\;6000\;W/m^2$. The ratio of ($PM_{10}$/CO) obtained at low photochemical activity regime was used as an index of tracer for the estimation of secondary $PM_{10}$ at higher photochemical activity regimes. The results show that the estimated secondary $PM_{10}$ concentrations for moderate and high photochemical regimes are found to be 18.8% ($10.9\;{\mu}g/m^3$), and 35.0% ($26.2\;{\mu}g/m^3$), respectively. Diurnal variation of secondary $PM_{10}$ for the moderate photochemical regime shows weak but noticeable patterns. However, the highly activated photochemical regime shows strong diurnal variations of secondary $PM_{10}$ concentrations with the maximum value of $35.1\;{\mu}g/m^3$ at 1300LST.

• Journal of Korea Water Resources Association
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• v.44 no.9
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• pp.741-751
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• 2011

In this study, a methodology was devised to overcome that difficulty for thermal stratification modeling using EFDC. For the increase of reproductability for thermal stratification analysis, the effect of parameter such as distribution of solar radiation, depth of active bed temperature layer, heat transfer coefficients were analyzed. The simulation period was from June to December in 2005 and statistical index is used to analyze the model results. The results showed that distribution of solar radiation is zero and depth of active bed temperature layer is 10 m are suitable for simulation of thermal stratification in Yongdam Dam reservoir. This study results can be used for guideline to analyze the thermal stratification of large dam reservoir in Korea.

• Journal of agricultural medicine and community health
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• v.7 no.1
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• pp.80-89
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• 1982

In order to observe the endemic status of Clonrchis sinensis infection in Samrangjin eup, Milyang gun, Kyongsang nam do, a total number of 294 stool specimens were collected from the inhabitants who lived in the basin of the Nakdong river. The specimens were examined by cellophane thick smear technique and Stoll's egg dilution technique during the period from May to June 1983. The epidemiological status was analysed statistically by the regression equation and catalytic curv with the results obtained from this area. The results are as follows : 1) The infestation rate of Clonorchis sinensis in 294 was 49.0%. 2) No remarkable difference was observed between male (52.5%) and female (44.8%). 3) Average E.P.G. by Stoll's egg dilution technique in this area was 9,597. 4) The degree of the intensity of Clonorchis infection by E.P.G. count was distributed as 10.5% in very heavy infection, 14.7% in heavy infection, 47.4% in moderate infection, and 29.3% in light infection. 5) The intensity of endemicity in this area was represented with the regression equation calculated with the cumulative percentages of E.P.G. counts. Regression equation was y=3.40+1.23 log x and Cs. $D._{50}$ was 19.99. 6) The two stage catalytic model was applied and the calculation lead to the equation $y=1296(e^{-0.008t}-e^{-0.035t})$ ; a=0.035 > b=0.008 in this surveyed area. 7) Other helminthic infection rate in this area was 7.1% in A. lumbricoides, 9.5% in T. trichiura and 4.1% in E. vermicularis respectively.

• Journal of Environmental Science International
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• v.29 no.8
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• pp.809-818
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• 2020

To conserve the population of a hesperiid butterfly, Leptalina unicolor, inhabiting the protected areas of Jaeyaksan, we provide ecological information on their behavior and propose habitat conservation measures, such as the creation of alternative habitats based on comprehensive information. The behavioral study used a method of re-capture after releasing the butterfly with enamel marks on the wings. Adult behaviors were shown in four patterns: flying, settling on a plant, nectar absorption, and water absorption. Both males and females had the highest proportion of flight movement overall; however, males had a slightly higher proportion of flight movement. As for duration, females spent more time settling on plants to select spawning sites, and males seemed to take a longer time for water absorption activities, to absorb minerals needed to form the spermatophore. The average travel distance of butterflies was 27.5 m for females and 46.7 m for males, with daily activity ranges from 11.2 m to 43.8 m, and 21.4 to 57.6 m, respectively. The most important condition to preserve the habitat of Leptalina unicolor is to maintain the community of Miscanthus sinensis, a food plant. Additionally, because this butterfly has a high rate of water absorption activities, wetlands should be maintained.

• Journal of the Korean Data and Information Science Society
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• v.28 no.6
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• pp.1447-1456
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• 2017

Accurate inference of parameters of a stochastic rainfall generation model is essential to improve the applicability of the rainfall generation model which modeled the rainfall process and the structure of rainfall events. In this study, the model parameters of a stochastic rainfall generation model, NSRPM (Neyman-Scott rectangular pulse model), were estimated using DFP (Davidon-Fletcher-Powell), GA (genetic algorithm), Nelder-Mead, and DE (differential evolution) methods. Summer season hourly rainfall data of 20 rainfall observation sites within the Nakdong river basin from 1973 to 2017 were used to estimate parameters and the regional applicability of inference methods were analyzed. Overall results demonstrated that DE and Nelder-Mead methods generate better results than that of DFP and GA methods.

• Journal of the Korean Geotechnical Society
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• v.21 no.6
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• pp.5-18
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• 2005

In this study, the influence of progressive consolidation from the drainage boundary on the subsequent process of consolidation was investigated. Analytical theory and numerical program f3r consolidation of clayey soil were developed based on finite difference method, in which spatial variation of permeability and volume compressibility were implemented. And model ground with normally consolidated clayey soils and a vertical drain at its center were simulated. Various types of soils with different relations between coefficient of volume compressibility and permeability and void ratio were applied. Also numerical simulations based on the properties of the normally consolidated clay at Nakdong River basin and reconstituted kaolinite soil were performed to recognize its practical impact. Consequently, it is found out that retardation of consolidation induced by progressive consolidation is very important to understand consolidation behavior on field conditions and its effect is remarkable at the initial state of consolidation, and increases with plasticity index and applied load.

• Preventive Nutrition and Food Science
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• v.15 no.2
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• pp.113-119
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• 2010

The aim of this study was to investigate changes in 7 nutritional components (fatty acid, protein, fat, ash, total dietary fiber (TDF), $\gamma$-aminobutyric acid (GABA), and $\gamma$-oryzanol) of paddy rice (PR) and brown rice (BR) throughout the germination process, as measured at different shoot lengths (10 mm, 20 mm, and 30 mm). With the increase of shoot length, the nutritional components' concentrations increased, as compared to the concentrations measured before germination. Moreover, BR exhibited higher GABA, $\gamma$-oryzanol, and protein than PR. Among the components, TDF, GABA, and $\gamma$-oryzanol showed significant concentration differences throughout germination, while the others exhibited only slight variations. In particular, GABA and $\gamma$-oryzanol were predominantly increased in grains of 10 mm shoot length. These compounds might prove to be important factors from germinated rice. Additionally, the germinated cultivar 'Keunnun' might also prove to be a very important food source, owing to its high GABA and $\gamma$-oryzanol contents. These results suggest that variations in nutritional components related to the increase of shoot length may prove to be important when considering the beneficial aspects of rice on human health.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.453-453
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• 2011

21세기에 들어 홍수의 규모가 대형화 되었고, 그 발생빈도 및 강도도 증가하고 있다. 최근에는 지구온난화가 지속화되면서 전 세계적으로 높은 강도의 기상이변들이 속출하고 있고, 이러한 이상기후에 따른 태풍, 집중호우 등의 대규모 호우로 인해 댐 및 제방 등의 수공구조물 붕괴와 같은 비상상황이 초래 될 수 있다. 이와 같은 피해들을 통해 홍수 침수 범위의 예측, 분석을 통한 홍수위험 및 다양한 홍수위험지도 작성의 필요성이 대두되었고, 실제로 국가 차원의 홍수위 험지도가 제작되고 있다. 특히, 홍수 위험도 분석에 있어서 홍수에 노출된 지역의 인구수, 홍수에 노출된 지역에서의 경제적 활동의 형태, 홍수가 발생했을 때 2차적 피해를 불러올 수 있는 설비 등을 나타내는 홍수 취약도(Flood Vulnerability)에 대한 정량적 평가는 홍수위험지표 및 홍수위험강도 등에 의한 Flood Risk 개념을 기반으로 한 홍수위험지도 제작을 위해 매우 중요한 사항이라 할 수 있다. 그러나 현재까지의 홍수취약도 산정방법은 방법론적인 면에 있어 다소 단순하고, 직관에 의한 위험도의 분류가 이루어지고 있는 실정이다. 또한 취약도 지표의 산정과정이 전문가의 의견에 의존하는 경우가 많아 홍수 취약도 선정과정과 가중치 결정과정에 전문가들의 주관이 개입되는 등 홍수위험지표의 정량화에 어려움을 겪는 경우가 많다. 본 연구에서는 위와 같은 문제를 극복하기 위해 Flood Risk Mapping 기술의 적용에 있어 중요한 요소인 홍수취약도를 다기준의사결정법에 의해 산정하고, 국내 낙동강 유역에 대해 행정구역별로 세분화된 홍수위험지도 제작을 위한 취약도 지표를 산정하고자 하였다. 이를 위해 다기준의사결정법중의 하나 인 PROMEETEE와 ELECTRE를 이용하여 민감도, 노출도, 저감성 지표를 낙동강 유역에 대해 정량화하여 도시하였다. 본 연구결과를 통해 홍수위험지표 및 지수들의 결합에 대한새로운 방법론을 제시하고, 그에 따른 지도화 기법을 확립할 수 있을 것으로 기대된다.

• Journal of the Korean Association of Geographic Information Studies
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• v.10 no.4
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• pp.22-34
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• 2007

The purpose of this study is to evaluate landscape characteristics of watersheds in the Nakdong River Basin for identifying the groups of watershed with similar landcover patterns by using Geographic Information System and statistical technique. According to the results based on the cluster analysis using cluster analysis tool in the ArcGIS 8.3 program, 22 sub-watersheds were classified into three types; "Forest watershed", "Agriculture watershed", and "Urban watershed". In the forest watershed that has the least potential of ecological disturbances by human, a forest management approach based on geographic conditions and coverage types, etc., should be developed to sustain the ecological and environmental functions of forest. For the agriculture watershed, environmental-friendly agricultural techniques should be performed in the particular enhancement of riparian buffer zone to the prevent direct inflow of soils, fertilizers, and other chemicals into the stream network. Finally, in the urban watershed, an environmental-friendly plan that may increase the ratio of pervious surface and amount of green-space to should be reserved.