• Restorative Dentistry and Endodontics
• /
• 제24권1호
• /
• pp.40-48
• /
• 1999

This study was designed to examine the specificities of the designed primers for F. nucleatum and F. necrophorum and to compare the PCR results using clinical samples with those of the anaerobic culture method. F. nucleatum and F. necrophorum spp. are frequently isolated in infected root canals, and they are related to periapical diseases. F. nucleatum(VPI 10197) and F. necrophorum(ATCC 25286) were used as references for PCR reaction, and thirty five teeth with one canal and periapical lesion were used. The samples were cultured anaerobically and identified using Rapid ID 32A(BioMerieux Vitek, Inc., France) as biochemical battery. In the GenBank database, species-specific PCR primers(nuc1/nuc2 primers for F. nucleatum and nec1/nec2 primers for F. necrophorum) were designed from the 16S ribosomal DNA(rDNA) sequences of F. nucleatum(accession number M58683) and F. necrophorum(accession number AF044948). PCR procedures of F. nucleatum(VPI 10197) and F. necrophorum (ATCC 25286) were simulated on a computer software. Amplify(v.1.2${\beta}$ for Macintosh). 820 bps and 817 bps of nucleotides were expected, respectively. Using extracted DNAs with QiaAmp tissue kit(Qiagen co., Germany), PCR was done. The results were as follows : 1. The nuc1/nuc2 primers produced an amplicon of 820 bps and the nec1/nec2 primers produced an amplicon of 817 bps. 2. The nuc1/nuc2 primers and the nec1/nec2 primers were specific and did not react with species other than the designated ones(i.e. nuc1/nuc2 primers did not produce amplicons for F. necrophorum, and vice versa.). And the PCR products of Porphyromonas endodontalis(ATCC 35406), Porphyromonas gingivalis(ATCC 33277), Prevotella intermedia(ATCC 25611), and Prevotella nigrescens(ATCC 33563), frequently isolated in infected root canals and periapical lesions, were not amplified by the primers specific for Fusobacterium nucleatum and Fusobacterium necrophorum. 3. This method utilizing PCR could detect F. nucleatum and F. necrophorum in clinical samples, while anaerobic culture method could detect neither.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
• /
• pp.586-588
• /
• 2005

The MSC (Multi Spectral Camera) system is a remote sensing payload to obtain high resolution ground image. This system uses lossy image compression method for &Direct mission& that transmit whole image during one contact. But some image degradation occurred especially at high compression ratio. To reduce this degradation, the MSC uses NUC (Non-uniformity Correction) Unit. This unit correct CCD (Charge Coupled Device)'s high-frequency non-uniformity. So high frequency contents of image can be minimized and whole system SNR can be maximized. But NUC has some disadvantage either. It decreases entire system reliability by adding one electronic system. Adding NUC also led to difficulty of electronic design, assembly and testability. In this paper, the comparison is performed between on-orbit non-uniform correction and on ground correction. by evaluating NUC advantage for the point of view of image quality. Using real MSC parameter and proper model, considerable reference point for the system design came to possible.

• Bulletin of the Korean Chemical Society
• /
• 제30권12호
• /
• pp.2909-2912
• /
• 2009

The amines studied in this study are less reactive toward ethyl propiolate (3) in MeCN than in H$_2$O although they are 7 to 9 pK$_a$ units more basic in the aprotic solvent. The reactivity of morpholine and deuterated morpholine toward 3 is found to be identical, indicating that proton transfer occurs after rate-determining step (RDS). The fact that kinetic isotope effect is absent excludes a stepwise mechanism in which proton transfer occurs in RDS as well as a concerted mechanism in which nucleophilic attack and proton transfer occur concertedly through a 4-membered cyclic transition state (TS). Thus, the reactions have been concluded to proceed through a stepwise mechanism in which proton transfer occurs after RDS. Brønsted-type plots are linear with small ${\beta}_{nuc}$ values, i.e., ${\beta}_{nuc}$ = 0.29 in H$_2$O and ${\beta}_{nuc}$ = 0.51 in MeCN, indicating that bond formation is not advanced significantly in RDS. The small ${\beta}_{nuc}$ value also supports the conclusion drawn from the study of kinetic isotope effect.

• 한국식품과학회지
• /
• 제41권4호
• /
• pp.423-427
• /
• 2009

본 연구에서는 탈지대두 grits(DSG)와 B. subtilis NUC1균주로 발효시킨 탈지대두 발효물(FD)을 제조한 다음 열풍건조한 시료를 물 추출하여 이들 DSG 물추출물(DW)과 발효된 DSG 물추출물(FDW)의 항혈전 활성과 콜레스테롤 저하 효과를 알아보았다. 그 결과, FDW는 DW보다 항혈전 활성 및 혈소판의 응고 억제 효과가 더 우수하였으며, 유의적으로 높은 HMG-CoA reductase 저해활성을 가졌다. 그러나 DW와 FDW 처리군 둘다 HepG2 세포를 이용한 콜레스테롤 생합성 억제능을 보였고, 대조군에 비해 세포 내의 콜레스테롤 함량을 유의적으로 감소시켰다. 그리고 콜레스테롤 흡착능에서는 FDW만 약 90% 정도의 높은 흡착능을 보였고, DW는 콜레스테롤 흡착능을 보이지 않았다. 따라서 발효된 DSG는 원료인 DSG 보다 항혈전 및 콜레스테롤 개선 효과가 더 우수한 것으로 생각되었다.

• 센서학회지
• /
• 제17권2호
• /
• pp.151-155
• /
• 2008

In this paper, it was investigated that the pattern recognition of multiplication environment of lactic acid bacteria in the process of curd yogurt preparation using household fermentation system, which was manufactured by combining incubator with sensor module, data processing circuit and computer. It will be sufficiently applicable to determine the maximum ratio of the amount of air to mixed milk for preparation of high quality yogurt.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 2004년도 Proceedings of ISRS 2004
• /
• pp.471-474
• /
• 2004

Not all CCD pixels generate uniform value for the uniform radiance due to the different process of manufacture and each pixel characteristics. And the image data compression is essential in the real time image transmission because of the high line rate and the limited RF bandwidth. This pixel's nonuniformity and the loss compression make CCD pixel correction necessary in on-orbit condition. In the MSC system, the NUC unit, which is a part of MSC PMU, is charge of the correction for CCD each pixel. The correction is performed with the gain and the offset table for the each pixel and the each TDI mode. These correction tables are generated and programmed in the PMU Flash memory through the various image data tests at the ground test. Besides, they can be uploaded from ground station after onorbit calibration. This paper describes the principle of the table generation and the test way of the non-uniformity after NUC

• Journal of Microbiology
• /
• 제45권4호
• /
• pp.297-304
• /
• 2007

The detection of virulence factors of Aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. In this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and Aeromonas species were isolated and identified. For rapid detection of six virulence factors of isolated Aeromonas, a hexaplex-polymerase chain reaction (hexaplex-PCR) assay was used. The detected virulence factors include aerolysin (aer), GCAT (gcat), serine protease (ser), nuclease (nuc) lipase (lip) and lateral flagella (laf). The dominant strain found in our isolates was Aeromonas sobria, and the dominant virulence factors were aer and nuc for all seasons. We confirmed that A. sobria and two of the virulence genes (aer and nuc) are related. We proposed a method by which one can identify the major strains of Aeromonas: A. hydrophila, A. sobria, A. caviae, and A. veronii, using hexaplex-PCR.

• 한국컴퓨터정보학회논문지
• /
• 제23권11호
• /
• pp.1-7
• /
• 2018

In this paper, we propose soft dead pixels detection method by analysing different response at hot and cold images. Abnormal pixels are able to effect detecting a small target. It also makes confusing real target or not cause of changing target size. Almost exist abnormal pixels after image signal processing even if dead pixels are removed by dead pixel compensation are called soft dead pixels. They are showed defect in final image. So removing or compensating dead pixels are very important for detecting object. The key idea of this proposed method, detecting dead pixels, is that most of soft deads have different response characteristics between hot image and cold image. General infrared cameras do NUC to remove FPN. Working 2-reference NUC must be needed getting data, hot & cold images. The way which is proposed dead pixel detection is that we compare response, NUC gain, at each pixel about two different temperature images and find out dead pixels if the pixels exceed threshold about average gain of around pixels.

• 한국균학회지
• /
• 제42권4호
• /
• pp.341-343
• /
• 2014

Camarops ustulinoides (Henn.) Nannf., belonging to the family Boliniaceae (Order Boliniales), is reported as new to Korea. The morphological characteristics and molecular phylogenetic analysis based on the nucLSU region sequence confirm the recognition of this taxon. Illustrated descriptions of C. ustulinoides are provided.

• 항공우주기술
• /
• 제11권1호
• /
• pp.98-102
• /
• 2012

비냉각형 열상 시스템에 대한 관심이 국방 및 항공우주 분야에서 증가하고 있다. 특히, 국방분야 무기체계에서는 무인화 및 주야간 적군 탐지를 위한 요소기술로 활용되고 있다. 비냉각형 열상 시스템의 연구 분야 중 저비용, 저전력, 소형화를 위한 비냉각형 TEC(Thermal Electric Cooler)-less 열상 시스템에 대한 연구가 활발히 진행되고 있다. 그러나 TEC-less로 운영하기 위해서는 최적화된 불균일보정 계수의 추출 및 적용이 요구된다. 본 논문에서는 TEC-less로 최적화 된 보정 계수 획득 방법으로 선형 보간법을 이용한 보정 계수 추정 방법인 DCCE-LI(Dynamic Calibration Coefficient Estimation with Linear Interpolation)을 제안하고, 실험을 통해 제안 기법이 기존의 정적 보정 계수를 적용한 것에 비해 IR 영상 품질이 우수하고, 실시간 보정 계수 추정이 가능함을 보인다.